İstanbul Journal of Pharmacy

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DOI :10.26650/IstanbulJPharm.2019.0062   IUP :10.26650/IstanbulJPharm.2019.0062    Full Text (PDF)

Determination of chlorpheniramine enantiomers in pharmaceutical formulations by HPLC on chiral column with PDA detection

Gizem Erensoy, Duygu Taşkın, Gamze Özgül Artuç, Elif Özdemir, Sumru Özkırımlı

Background and Aims: An HPLC method with photodiode array detector on a chiral column was proposed for enantioselective determination of chlorpheniramine (CLP) enantiomers in dosage forms.

Methods: The enantioselective determination was achieved on amylose tris(3,5-dimethylphenylcarbamate) column, using n-hexane-(propan-2-ol)-diethylamine (97.5:2.5:0.025, v/v/v) mobile phase. The peaks were detected at 258 nm. Diphenhydramine was used as an internal standard (IS). A new sample preparation procedure was developed to avoid the interference of the other ingredients present in the formulations.

Results: Limit of quantification of the proposed method was 0.88 and 1.31 µg/mL for S-(+)-CLP and R-(-)-CLP, respectively.

Conclusion: The method is linear, sensitive, specific and can be used for the enantioselective assay of CLP enantiomers in pharmaceutical formulations.

Keywords: Chlorpheniramine, amylose tris(3, 5-dimethyl phenylcarbamate), enantioselective determination, HPLC-PDA, chirality

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References

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APA

Erensoy, G., Taşkın, D., Özgül Artuç, G., Özdemir, E., & Özkırımlı, S. (2020). Determination of chlorpheniramine enantiomers in pharmaceutical formulations by HPLC on chiral column with PDA detection. İstanbul Journal of Pharmacy, 50(2), 149-152. https://doi.org/10.26650/IstanbulJPharm.2019.0062


AMA

Erensoy G, Taşkın D, Özgül Artuç G, Özdemir E, Özkırımlı S. Determination of chlorpheniramine enantiomers in pharmaceutical formulations by HPLC on chiral column with PDA detection. İstanbul Journal of Pharmacy. 2020;50(2):149-152. https://doi.org/10.26650/IstanbulJPharm.2019.0062


ABNT

Erensoy, G.; Taşkın, D.; Özgül Artuç, G.; Özdemir, E.; Özkırımlı, S. Determination of chlorpheniramine enantiomers in pharmaceutical formulations by HPLC on chiral column with PDA detection. İstanbul Journal of Pharmacy, [Publisher Location], v. 50, n. 2, p. 149-152, 2020.


Chicago: Author-Date Style

Erensoy, Gizem, and Duygu Taşkın and Gamze Özgül Artuç and Elif Özdemir and Sumru Özkırımlı. 2020. “Determination of chlorpheniramine enantiomers in pharmaceutical formulations by HPLC on chiral column with PDA detection.” İstanbul Journal of Pharmacy 50, no. 2: 149-152. https://doi.org/10.26650/IstanbulJPharm.2019.0062


Chicago: Humanities Style

Erensoy, Gizem, and Duygu Taşkın and Gamze Özgül Artuç and Elif Özdemir and Sumru Özkırımlı. “Determination of chlorpheniramine enantiomers in pharmaceutical formulations by HPLC on chiral column with PDA detection.” İstanbul Journal of Pharmacy 50, no. 2 (May. 2025): 149-152. https://doi.org/10.26650/IstanbulJPharm.2019.0062


Harvard: Australian Style

Erensoy, G & Taşkın, D & Özgül Artuç, G & Özdemir, E & Özkırımlı, S 2020, 'Determination of chlorpheniramine enantiomers in pharmaceutical formulations by HPLC on chiral column with PDA detection', İstanbul Journal of Pharmacy, vol. 50, no. 2, pp. 149-152, viewed 6 May. 2025, https://doi.org/10.26650/IstanbulJPharm.2019.0062


Harvard: Author-Date Style

Erensoy, G. and Taşkın, D. and Özgül Artuç, G. and Özdemir, E. and Özkırımlı, S. (2020) ‘Determination of chlorpheniramine enantiomers in pharmaceutical formulations by HPLC on chiral column with PDA detection’, İstanbul Journal of Pharmacy, 50(2), pp. 149-152. https://doi.org/10.26650/IstanbulJPharm.2019.0062 (6 May. 2025).


MLA

Erensoy, Gizem, and Duygu Taşkın and Gamze Özgül Artuç and Elif Özdemir and Sumru Özkırımlı. “Determination of chlorpheniramine enantiomers in pharmaceutical formulations by HPLC on chiral column with PDA detection.” İstanbul Journal of Pharmacy, vol. 50, no. 2, 2020, pp. 149-152. [Database Container], https://doi.org/10.26650/IstanbulJPharm.2019.0062


Vancouver

Erensoy G, Taşkın D, Özgül Artuç G, Özdemir E, Özkırımlı S. Determination of chlorpheniramine enantiomers in pharmaceutical formulations by HPLC on chiral column with PDA detection. İstanbul Journal of Pharmacy [Internet]. 6 May. 2025 [cited 6 May. 2025];50(2):149-152. Available from: https://doi.org/10.26650/IstanbulJPharm.2019.0062 doi: 10.26650/IstanbulJPharm.2019.0062


ISNAD

Erensoy, Gizem - Taşkın, Duygu - Özgül Artuç, Gamze - Özdemir, Elif - Özkırımlı, Sumru. “Determination of chlorpheniramine enantiomers in pharmaceutical formulations by HPLC on chiral column with PDA detection”. İstanbul Journal of Pharmacy 50/2 (May. 2025): 149-152. https://doi.org/10.26650/IstanbulJPharm.2019.0062



TIMELINE


Submitted04.12.2019
Accepted17.04.2020
Published Online27.08.2020

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