İstanbul Journal of Pharmacy

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DOI :10.26650/IstanbulJPharm.2022.877867   IUP :10.26650/IstanbulJPharm.2022.877867    Full Text (PDF)

Determination of diazepam in human plasma by developed and validated a high-performance liquid chromatographic ultraviolet method

Emrah Dural, Beyza Nur Kaya

Background and Aims: Diazepam is accepted as a safer drug to medicate in many serious cases, acting as an anticonvulsant, an anxiolytic and a treatment for many types of poisoning. Monitoring it is important in achieving successful treatment and reducing the risk of toxic effects. In this study, it is aimed to develop and validate a sensitive, repeatable, and reliable method based on high-performance liquid chromatographic analysis for the determination in human plasma. Methods: Separation was carried out using a reverse-phase C18 column (4.0 mm x 150 mm, 3 μm) at 30 °C. The solution was prepared with a 10 mM phosphate buffer and acetonitrile (1:1, v/v) was employed as a mobile phase at the isocratic flow with 0.5 mL/min rate. Quantification was applied at 230 nm. A solid-phase extraction method was established and optimized, which was then used in the preparation of the plasma (0.5 mL) samples to the analysis. Results: The method was found to be linear (r2 = 0.9805) between 100 and 1200 ng/mL. The analysis run was ≤12 min. Intraday and inter-day accuracy were found between -5.78 and 5.93 and precision was ≤1.82%. The limit of detection and quantification were calculated as 20.42 and 61.86 ng/mL, respectively. Recovery was found between the range of 95.12% and 106.83%. The method was determined to be robust according to changes in UV, mobile phase organic solvent content, mobile phase pH, column temperature, and operator. Conclusion: This simple, sensitive and reliable method is suggested for accredited-reference laboratories working on the therapeutic drug monitorization and/or overdose-toxicological quantified analysis of diazepam in human plasma.

Keywords: Diazepam, Solid-phase extraction, Method validation, High-performance liquid chromatography-ultraviolet detection

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APA

Dural, E., & Kaya, B.N. (2022). Determination of diazepam in human plasma by developed and validated a high-performance liquid chromatographic ultraviolet method. İstanbul Journal of Pharmacy, 52(1), 37-46. https://doi.org/10.26650/IstanbulJPharm.2022.877867


AMA

Dural E, Kaya B N. Determination of diazepam in human plasma by developed and validated a high-performance liquid chromatographic ultraviolet method. İstanbul Journal of Pharmacy. 2022;52(1):37-46. https://doi.org/10.26650/IstanbulJPharm.2022.877867


ABNT

Dural, E.; Kaya, B.N. Determination of diazepam in human plasma by developed and validated a high-performance liquid chromatographic ultraviolet method. İstanbul Journal of Pharmacy, [Publisher Location], v. 52, n. 1, p. 37-46, 2022.


Chicago: Author-Date Style

Dural, Emrah, and Beyza Nur Kaya. 2022. “Determination of diazepam in human plasma by developed and validated a high-performance liquid chromatographic ultraviolet method.” İstanbul Journal of Pharmacy 52, no. 1: 37-46. https://doi.org/10.26650/IstanbulJPharm.2022.877867


Chicago: Humanities Style

Dural, Emrah, and Beyza Nur Kaya. “Determination of diazepam in human plasma by developed and validated a high-performance liquid chromatographic ultraviolet method.” İstanbul Journal of Pharmacy 52, no. 1 (Apr. 2024): 37-46. https://doi.org/10.26650/IstanbulJPharm.2022.877867


Harvard: Australian Style

Dural, E & Kaya, BN 2022, 'Determination of diazepam in human plasma by developed and validated a high-performance liquid chromatographic ultraviolet method', İstanbul Journal of Pharmacy, vol. 52, no. 1, pp. 37-46, viewed 25 Apr. 2024, https://doi.org/10.26650/IstanbulJPharm.2022.877867


Harvard: Author-Date Style

Dural, E. and Kaya, B.N. (2022) ‘Determination of diazepam in human plasma by developed and validated a high-performance liquid chromatographic ultraviolet method’, İstanbul Journal of Pharmacy, 52(1), pp. 37-46. https://doi.org/10.26650/IstanbulJPharm.2022.877867 (25 Apr. 2024).


MLA

Dural, Emrah, and Beyza Nur Kaya. “Determination of diazepam in human plasma by developed and validated a high-performance liquid chromatographic ultraviolet method.” İstanbul Journal of Pharmacy, vol. 52, no. 1, 2022, pp. 37-46. [Database Container], https://doi.org/10.26650/IstanbulJPharm.2022.877867


Vancouver

Dural E, Kaya BN. Determination of diazepam in human plasma by developed and validated a high-performance liquid chromatographic ultraviolet method. İstanbul Journal of Pharmacy [Internet]. 25 Apr. 2024 [cited 25 Apr. 2024];52(1):37-46. Available from: https://doi.org/10.26650/IstanbulJPharm.2022.877867 doi: 10.26650/IstanbulJPharm.2022.877867


ISNAD

Dural, Emrah - Kaya, BeyzaNur. “Determination of diazepam in human plasma by developed and validated a high-performance liquid chromatographic ultraviolet method”. İstanbul Journal of Pharmacy 52/1 (Apr. 2024): 37-46. https://doi.org/10.26650/IstanbulJPharm.2022.877867



TIMELINE


Submitted10.02.2021
Accepted17.02.2022
Published Online28.04.2022

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