European Journal of Biology

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DOI :10.26650/EurJBiol.2020.0013   IUP :10.26650/EurJBiol.2020.0013    Full Text (PDF)

Colchicine Quantification in Salt Stress Treated Culture of Colchicum luteum Baker by High Pressure Liquid Chromatography

Mehpara Maqsood, Mir Khusrau, Zahoor Ahmad Kaloo, Tareq Ahmad Wani, Abdul Mujib

Objective: The aim of the present work was to develop a protocol for season independent propagation of Colchicum luteum and enrichment of colchicine yield under salt stress. Materials and Methods: C. luteum was collected from Kashmir, India and was cultured in vitro. The callus was exposed to different NaCl (salt) treatments and the yield of colchicine was quantified by high pressure liquid chromatography (HPLC). Results: Different explants viz. seeds, leaves, anthers and corm were used and the callus was only induced from corm segments. The callus induction and proliferation were best achieved on Murashige and Skoog medium supplemented with 2.0 mg L⁻¹ 2, 4-D + 4.0 mg L⁻¹ BAP. Direct and indirect plant regeneration from corm was observed in 2.0 mg L⁻¹ BAP + 2.0 mg L⁻¹ NAA added medium. The addition of adjuvants like activated charcoal and citric acid was noted to be less efficient for improving callus growth. Induced callus was elicitated with different NaCl concentrations (T₀ = without NaCl, T₁  = 25, T₂ = 50, T ₃  = 75, and T4 =100 mM). The yield of colchicine was quantified by HPLC at periodic intervals (2, 4, and 6 weeks). All the used levels improved colchicine yield, but the content was maximum at T ₃  (871.4 ng mg-1 DWB). This is the first report of callus induction and plant regeneration from corm and the quantification of colchicine under salt stress in C. luteum. Conclusion: Sodium chloride is thus a potential inducer and elicitor in improving colchicine yield in C. luteum.

Keywords: Colchicum luteum, exudates, regeneration, colchicine, in vitro cultures, stress

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APA

Maqsood, M., Khusrau, M., Kaloo, Z.A., Wani, T.A., & Mujib, A. (2020). Colchicine Quantification in Salt Stress Treated Culture of Colchicum luteum Baker by High Pressure Liquid Chromatography. European Journal of Biology, 79(2), 67-74. https://doi.org/10.26650/EurJBiol.2020.0013


AMA

Maqsood M, Khusrau M, Kaloo Z A, Wani T A, Mujib A. Colchicine Quantification in Salt Stress Treated Culture of Colchicum luteum Baker by High Pressure Liquid Chromatography. European Journal of Biology. 2020;79(2):67-74. https://doi.org/10.26650/EurJBiol.2020.0013


ABNT

Maqsood, M.; Khusrau, M.; Kaloo, Z.A.; Wani, T.A.; Mujib, A. Colchicine Quantification in Salt Stress Treated Culture of Colchicum luteum Baker by High Pressure Liquid Chromatography. European Journal of Biology, [Publisher Location], v. 79, n. 2, p. 67-74, 2020.


Chicago: Author-Date Style

Maqsood, Mehpara, and Mir Khusrau and Zahoor Ahmad Kaloo and Tareq Ahmad Wani and Abdul Mujib. 2020. “Colchicine Quantification in Salt Stress Treated Culture of Colchicum luteum Baker by High Pressure Liquid Chromatography.” European Journal of Biology 79, no. 2: 67-74. https://doi.org/10.26650/EurJBiol.2020.0013


Chicago: Humanities Style

Maqsood, Mehpara, and Mir Khusrau and Zahoor Ahmad Kaloo and Tareq Ahmad Wani and Abdul Mujib. “Colchicine Quantification in Salt Stress Treated Culture of Colchicum luteum Baker by High Pressure Liquid Chromatography.” European Journal of Biology 79, no. 2 (Apr. 2024): 67-74. https://doi.org/10.26650/EurJBiol.2020.0013


Harvard: Australian Style

Maqsood, M & Khusrau, M & Kaloo, ZA & Wani, TA & Mujib, A 2020, 'Colchicine Quantification in Salt Stress Treated Culture of Colchicum luteum Baker by High Pressure Liquid Chromatography', European Journal of Biology, vol. 79, no. 2, pp. 67-74, viewed 23 Apr. 2024, https://doi.org/10.26650/EurJBiol.2020.0013


Harvard: Author-Date Style

Maqsood, M. and Khusrau, M. and Kaloo, Z.A. and Wani, T.A. and Mujib, A. (2020) ‘Colchicine Quantification in Salt Stress Treated Culture of Colchicum luteum Baker by High Pressure Liquid Chromatography’, European Journal of Biology, 79(2), pp. 67-74. https://doi.org/10.26650/EurJBiol.2020.0013 (23 Apr. 2024).


MLA

Maqsood, Mehpara, and Mir Khusrau and Zahoor Ahmad Kaloo and Tareq Ahmad Wani and Abdul Mujib. “Colchicine Quantification in Salt Stress Treated Culture of Colchicum luteum Baker by High Pressure Liquid Chromatography.” European Journal of Biology, vol. 79, no. 2, 2020, pp. 67-74. [Database Container], https://doi.org/10.26650/EurJBiol.2020.0013


Vancouver

Maqsood M, Khusrau M, Kaloo ZA, Wani TA, Mujib A. Colchicine Quantification in Salt Stress Treated Culture of Colchicum luteum Baker by High Pressure Liquid Chromatography. European Journal of Biology [Internet]. 23 Apr. 2024 [cited 23 Apr. 2024];79(2):67-74. Available from: https://doi.org/10.26650/EurJBiol.2020.0013 doi: 10.26650/EurJBiol.2020.0013


ISNAD

Maqsood, Mehpara - Khusrau, Mir - Kaloo, ZahoorAhmad - Wani, TareqAhmad - Mujib, Abdul. “Colchicine Quantification in Salt Stress Treated Culture of Colchicum luteum Baker by High Pressure Liquid Chromatography”. European Journal of Biology 79/2 (Apr. 2024): 67-74. https://doi.org/10.26650/EurJBiol.2020.0013



TIMELINE


Submitted08.04.2020
Accepted26.09.2020
Published Online13.12.2020

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