Research Article


DOI :10.26650/EurJBiol.2020.0021   IUP :10.26650/EurJBiol.2020.0021    Full Text (PDF)

Expression of blaA and blaB and Susceptibility to Penicillins and Cephalosporins in Yersinia enterocolitica from Different Foods

Fatma ÖzdemirSeza ArslanHafize Gizem Ertürk

Objective: Yersinia enterocolitica which is an important foodborne pathogen causing illness in humans is an extremely heterogeneous species consisting of different subtypes. It has the intrinsic resistance to β-lactam antibiotics because of the production of β-lactamases, BlaA and BlaB. Y. enterocolitica exhibits variable susceptibilities to β-lactams. Materials and Methods: The expression of the blaA and blaB genes by polymerase chain reaction, and the susceptibility to some β-lactams including penicillins and cephalosporins using the broth microdilution and disk diffusion methods were determined. A total of 18 Y. enterocolitica isolates were examined. Results: Overall, 33.3% of these isolates carried the blaA and blaB genes, all of which were recovered from chicken meat. The wide range of MIC for ampicillin (≤2-128 µg/mL) and ceftazidime (≤0.0625-2 µg/mL) was also observed. Of the Y. enterocolitica isolates, 55.6% were resistant to ampicillin (≥32 µg/mL) while the remaining isolates (44.4%) were susceptible to ampicillin (≤ 8 µg/mL). All isolates were susceptible to ceftazidime at the concentration tested. According to the disk diffusion test, 55.6% and 33.3% of the isolates were resistant to ticarcillin and cefoxitin, respectively. No resistance to piperacillin and ceftriaxone was found. Conclusion: The results showed that the presence of the blaA and blaB genes and intrinsic resistance against penicillins and cephalosporins were variable among Y. enterocolitica food isolates. Furthermore, the blaA and blaB genes were expressed in most of the resistant isolates to β-lactams, which may indicate the contribution of the genes to the drug resistance.


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References

  • Carniel E, Autenrieth I, Cornelis G, Fukushima H, Guinet F, Isberg R, et al. Y. enterocolitica and Y. pseudotuberculosis. Dworkin M, Falkow S, Rosenberg E, Schleifer, KH, Stackebrandt E, editors. The Prokary-otes. New York, NY: Springer; 2006.p.270-398. google scholar
  • Sharma S, Mittal S, Mallik S, Virdi JS. Molecular characterization of 0-lactamase genes blaA and blaB of Yersinia enterocolitica biovar 1A. FEMS Microbiol Lett 2006; 257: 319-27. google scholar
  • Bhunia AK. Foodborne Microbial Pathogens: Mechanisms and Pathogenesis. New York, NY: Springer; 2008. google scholar
  • Logue CM, Sheridan JJ, Wauters G, McDowell DA, Blair IS. Yersinia spp. and numbers, with particular reference to Y. enterocolitica bio/ serotypes, occurring on Irish meat and meat products, and the in-fluence of alkali treatment on their isolation. Int J Food Microbiol 1996; 33: 257-74. google scholar
  • Capita R, Calleja CA, Prieto M, Fernandez MDCG, Moreno B. Inci-dence and pathogenicity of Yersinia spp. isolates from poultry in Spain. Food Microbiol 2002; 19: 295-301. google scholar
  • Soltan-Dallal MM, Tabarraie A, MoezArdalan K. Comparison of four methods for isolation of Yersinia enterocolitica from raw and pasteur-ized milk from northern Iran. Int J Food Microbiol 2004; 94: 87-91. google scholar
  • Van Damme I, Berkvens D, Botteldoorn N, Dierick K, Wits J, Pochet B, et al. Evaluation of the ISO 10273:2003 method for the isolation of human pathogenic Yersinia enterocolitica from pig carcasses and minced meat. Food Microbiol 2013; 36: 170-5. google scholar
  • Martins BTF, Botelho CV, Silva DAL, Lanna FGPA, Grossi JL, Cam-pos-Galvao MEM, et al. Yersinia enterocolitica in a Brazilian pork production chain: Tracking of contamination routes, virulence and antimicrobial resistance. Int J Food Microbiol 2018; 276: 5-9. google scholar
  • Ray B. Fundamental Food Microbiology. 3rd ed. Boca Raton, Flori-da: CRC Press; 2004. google scholar
  • Preston MA, Brown S, Borczyk AA, Riley G, Krishnan C. Antimicro-bial susceptibility of pathogenic Yersinia enterocolitica isolated in Canada from 1972 to 1990. Antimicrob Agents Chemother 1994; 38: 2121-4. google scholar
  • Crowe M, Ashford K, Ispahani P. Clinical features and antibiotic treatment of septic arthritis and osteomyelitis due to Yersinia en-terocolitica. J Med Microbiol 1996; 45: 302-9. google scholar
  • Stock I. Natural susceptibility of Yersinia enterocolitica-like species to 0-lactam antibiotics. Rev Med Microbiol 2004; 15: 81-92. google scholar
  • Fabrega A, Vila J. Yersinia enterocolitica: pathogenesis, virulence and antimicrobial resistance. Enferm Infecc Microbiol Clin 2012; 30: 24-32. google scholar
  • Cornelis G, Abraham EP. 0- lactamases from Yersinia enterocolitica. J Gen Microbiol 1975; 87: 273-84. google scholar
  • Pham JN, Bell SM, Lanzarone JYM. A study of the 0-lactamases of 100 clinical isolates of Yersinia enterocolitica. J Antimicrob Chemo-ther 1991; 28: 19-24. google scholar
  • Sharma S, Ramnani P, Virdi JS. Detection and assay of 0-lactamases in clinical and non-clinical strains of Yersinia enterocolitica biovar 1A. J Antimicrob Chemother 2004; 54: 401-5. google scholar
  • Livermore DM. 0- Lactamases in laboratory and clinical resistance. Clin Microbiol 1995; 8: 557-84. google scholar
  • Stock I, Heisig P, Wiedemann B. Expression of 0-lactamases in Yersinia enterocolitica strains of biovars 2, 4 and 5. J Med Microbiol 1999; 48: 1023-7. google scholar
  • Stock I, Heisig P, Wiedemann B. 0-lactamase expression in Yersinia enterocolitica biovars 1A, 1B, and 3. J Med Microbiol 2000; 49: 403-8. google scholar
  • Bent ZW, Young GM. Contribution of BlaA and BlaB 0-lactamases to antibiotic susceptibility of Yersinia enterocolitica Biovar 1B. Anti-microb Agents Chemother 2010; 54: 4000-2. google scholar
  • Singhal N, Srivastava A, Kumar M, Virdi JS. Structural variabilities in 0-lactamase (blaA) of different biovars of Yersinia enterocolitica: implications for 0-lactam antibiotic and 0-lactamase inhibitor sus-ceptibilities. PloS One 2015; 10(4): e0123564. google scholar
  • Neubauer H, Hensel A, Aleksic S, Meyer H. Identification of Yersinia enterocolitica within the genus Yersinia. Syst Appl Microbiol 2000; 23: 58-62. google scholar
  • Bottone EJ, Bercovier H, Mollaret HH. Genus XLI. Yersinia. Garrity GM, Brenner DJ, Krieg NR, Staley JT, editors. Bergey’s manual of systematic bacteriology. New York, NY: Springer; 2005. p. 838-48. google scholar
  • Ausubel FM, Kingston RE, Brent R, Moore DD, Seidman J, Smith JA, et al. editors. Current protocols in molecular biology. New York, NY: Greene Publishing Associates & Wiley Interscience; 1991. google scholar
  • Meyer C, Stolle A, Fredriksson-Ahomaa M. Comparison of broth microdilution and disk diffusion test for antimicrobial resistance testing in Yersinia enterocolitica 4/O:3 strains. Microb Drug Resist 2011; 17: 479-84. google scholar
  • International Standard. Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices-Part 1: Broth micro-dilution reference method for testing the in vitro activity of antimicrobial agents against rapidly growing aerobic bacteria involved in infectious diseases. Geneva, Switzer-land; ISO 20776-1: 2019. google scholar
  • Clinical and Laboratory Standards Institute (CLSI), Performance standards for Antimicrobial Susceptibility Testing; Twenty-sixth Edition, in, Wayne, PA, USA, 2016. google scholar
  • Ye Q, Wu Q, Hu H, Zhang J, Huang H. Prevalence and characteri-zation of Yersinia enterocolitica isolated from retail foods in China. Food Control 2016; 61: 20-7. google scholar
  • Kot B, Piechota M, Jakubiak K. Virulence genes and antibiotic resis-tance of Yersinia enterocolitica strains isolated from children. EJBR 2017; 7: 366-73. google scholar
  • Singhal N, Kumar M, Virdi JS. Molecular analysis of 0-Lactamase genes to understand their differential expression in strains of Yersinia enterocolitica biotype 1A. Sci Rep 2014; 4:5270. google scholar
  • Pham JN, Bell SM, Martin L, Carniel E. The 0-lactamases and 0-lac-tam antibiotic susceptibility of Yersinia enterocolitica. J Antimicrob Chemother 2000; 46: 951-7. google scholar
  • Mittal S, Mallik S, Sharma S, Virdi JS. Characteristics of 0-lactamas-es and their genes (blaA and blaB) in Yersinia intermedia and Y. frederiksenii. BMC Microbiol 2007; 7:25. google scholar
  • Fabrega A, Balleste-Delpierre C, Vila J. Antimicrobial resistance in Yersinia enterocolitica. Chen C-Y, Yan X, Jackson CR, editors. Antimi-crobial Resistance and Food Safety; 2015.p.77-104. google scholar
  • Peng Z, Zou M, Li M, Liu D, Guan W, Hao Q, et al. Prevalence, anti-microbial resistance and phylogenetic characterization of Yersinia enterocolitica in retail poultry meat and swine feces in parts of Chi-na. Food Control 2018; 93: 121-8. google scholar
  • Kwaga J, Iversen JO. In vitro antimicrobial susceptibilities of Yersin-ia enterocolitica and related species isolated from slaughtered pigs and pork products. Antimicrob Agents Chemother 1990; 34: 2423-5. google scholar
  • Verbikova V, Borilova G, Babak V, Moravkova M. Prevalence, charac-terization and antimicrobial susceptibility of Yersinia enterocolitica and other Yersinia species found in fruits and vegetables from the European Union. Food Control 2018; 85: 161-7. google scholar
  • Bonke R, Wacheck S, Stuber E, Meyer C, Martlbauer E, Fredriks-son-Ahomaa M. Antimicrobial susceptibility and distribution of 0-lactamase A (blaA) and 0-lactamase B (blaB) genes in entero-pathogenic Yersinia species. Microb Drug Resist 2011; 17: 575-81. google scholar
  • Abdel-Haq NM, Papadopol R, Asmar BI, Brown WJ. Antibiotic sus-ceptibilities of Yersinia enterocolitica recovered from children over a 12-year period. Int J Antimicrob Agents 2006; 27: 449-52. google scholar
  • Weiner M. ERIC-based differentiation and antimicrobial suscepti-bility of Yersinia enterocolitica O:9 isolated from animals serolog-ically positive and negative for brucellosis. Bull Vet Inst Pulawy 2013; 57: 493-8. google scholar
  • Frazao MR, Andrade LN, Darini ALC, Falcao JP. Antimicrobial resis-tance and plasmid replicons in Yersinia enterocolitica strains isolat-ed in Brazil in 30 years. Braz J Infect Dis 2017; 21: 477-80. google scholar
  • Ye Q, Wu Q, Hu H, Zhang J, Huang H. Prevalence, antimicrobial re-sistance and genetic diversity of Yersinia enterocolitica isolated from retail frozen foods in China. FEMS Microbiol Lett 2015; 362: 1-7. google scholar
  • Baumgartner A, Kuffer M, Suter D, Jemni T, Rohner P. Antimicrobial resistance of Yersinia enterocolitica strains from human patients, pigs and retail pork in Switzerland. Int J Food Microbiol 2007; 115: 110-4. google scholar
  • Bonardi S, Bassi L, Brindani F, D’Incau M, Barco L, Carra E, et al. Prev-alence, characterization and antimicrobial susceptibility of Salmo-nella enterica and Yersinia enterocolitica in pigs at slaughter in Italy. Int J Food Microbiol 2013; 163: 248-57. google scholar

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APA

Özdemir, F., Arslan, S., & Ertürk, H.G. (2020). Expression of blaA and blaB and Susceptibility to Penicillins and Cephalosporins in Yersinia enterocolitica from Different Foods. European Journal of Biology, 79(2), 83-88. https://doi.org/10.26650/EurJBiol.2020.0021


AMA

Özdemir F, Arslan S, Ertürk H G. Expression of blaA and blaB and Susceptibility to Penicillins and Cephalosporins in Yersinia enterocolitica from Different Foods. European Journal of Biology. 2020;79(2):83-88. https://doi.org/10.26650/EurJBiol.2020.0021


ABNT

Özdemir, F.; Arslan, S.; Ertürk, H.G. Expression of blaA and blaB and Susceptibility to Penicillins and Cephalosporins in Yersinia enterocolitica from Different Foods. European Journal of Biology, [Publisher Location], v. 79, n. 2, p. 83-88, 2020.


Chicago: Author-Date Style

Özdemir, Fatma, and Seza Arslan and Hafize Gizem Ertürk. 2020. “Expression of blaA and blaB and Susceptibility to Penicillins and Cephalosporins in Yersinia enterocolitica from Different Foods.” European Journal of Biology 79, no. 2: 83-88. https://doi.org/10.26650/EurJBiol.2020.0021


Chicago: Humanities Style

Özdemir, Fatma, and Seza Arslan and Hafize Gizem Ertürk. Expression of blaA and blaB and Susceptibility to Penicillins and Cephalosporins in Yersinia enterocolitica from Different Foods.” European Journal of Biology 79, no. 2 (Mar. 2024): 83-88. https://doi.org/10.26650/EurJBiol.2020.0021


Harvard: Australian Style

Özdemir, F & Arslan, S & Ertürk, HG 2020, 'Expression of blaA and blaB and Susceptibility to Penicillins and Cephalosporins in Yersinia enterocolitica from Different Foods', European Journal of Biology, vol. 79, no. 2, pp. 83-88, viewed 28 Mar. 2024, https://doi.org/10.26650/EurJBiol.2020.0021


Harvard: Author-Date Style

Özdemir, F. and Arslan, S. and Ertürk, H.G. (2020) ‘Expression of blaA and blaB and Susceptibility to Penicillins and Cephalosporins in Yersinia enterocolitica from Different Foods’, European Journal of Biology, 79(2), pp. 83-88. https://doi.org/10.26650/EurJBiol.2020.0021 (28 Mar. 2024).


MLA

Özdemir, Fatma, and Seza Arslan and Hafize Gizem Ertürk. Expression of blaA and blaB and Susceptibility to Penicillins and Cephalosporins in Yersinia enterocolitica from Different Foods.” European Journal of Biology, vol. 79, no. 2, 2020, pp. 83-88. [Database Container], https://doi.org/10.26650/EurJBiol.2020.0021


Vancouver

Özdemir F, Arslan S, Ertürk HG. Expression of blaA and blaB and Susceptibility to Penicillins and Cephalosporins in Yersinia enterocolitica from Different Foods. European Journal of Biology [Internet]. 28 Mar. 2024 [cited 28 Mar. 2024];79(2):83-88. Available from: https://doi.org/10.26650/EurJBiol.2020.0021 doi: 10.26650/EurJBiol.2020.0021


ISNAD

Özdemir, Fatma - Arslan, Seza - Ertürk, HafizeGizem. Expression of blaA and blaB and Susceptibility to Penicillins and Cephalosporins in Yersinia enterocolitica from Different Foods”. European Journal of Biology 79/2 (Mar. 2024): 83-88. https://doi.org/10.26650/EurJBiol.2020.0021



TIMELINE


Submitted08.05.2020
Accepted09.07.2020
Published Online13.12.2020

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