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DOI :10.26650/eor.20210119   IUP :10.26650/eor.20210119    Full Text (PDF)

The antibacterial effects of vitamin D3 against mutans streptococci: an in vitro study

Manal Mohamed Mansour Almoudi, Alaa Sabah Hussein, Mohamed Ibrahim Abu Hassan, Hassanain Al Talib, Hasnah Begum Said Gulam Khan, Siti Arisya Binti Nazli, Nur Aina Efira Binti Effandy

Purpose This study aims to evaluate the antimicrobial effects of the cholecalciferol vitamin D3 against Streptococcus sobrinus (Strep. sobrinus) and Streptococcus mutans (Strep. mutans) bacteria in vitro that is considered the main causative bacteria in dental caries development. Materials and Methods The antimicrobial effects of vitamin D3 were evaluated against Strep. sobrinus and Strep mutans using the agar disc diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of vitamin D3 were determined using a microdilution method following the guidelines by the Clinical Laboratory Standards Institute (CLSI). Scanning electron microscope (SEM) was used to evaluate the morphological changes of bacterial cells following exposure to vitamin D3. Results Strep. sobrinus was more sensitive to vitamin D3 compared to Strep. mutans bacteria. The MIC values of vitamin D3 against Strep. sobrinus and Strep. mutans were 60 µg/ mL and 250 µg/mL respectively whereas the MBC values were 120 µg/mL and 500 µg/mL, respectively. Moreover, significant changes in the bacterial morphology were observed in treated bacterial cells with vitamin D3 as compared to the untreated control bacteria using SEM. Conclusion These findings suggested that vitamin D3 has excellent antimicrobial effects against Strep. sobrinus and Strep. mutans and may be considered as a promising compound in the prevention of dental caries in the future. Further research is recommended to elucidate the mechanism of vitamin D3 on these bacteria.

Keywords: Vitamin D3, Cholecalciferol, Streptococcus sobrinus, Streptococcus mutans, antibacterial effect

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References

  • 1. Wójcik D, Krzewska A, Szalewski L, Pietryka-Michałowska E, Szalewska M, Krzewski S, et al. Dental caries and vitamin D3 in children with growth hormone deficiency: A STROBE compliant study. Medicine. Medicine 2018;97. [CrossRef] google scholar
  • 2. Almoudi MM, Hussein AS, Abu Hassan MI, Schroth RJ. Dental caries and vitamin D status among children in Asia: A literature review. Pediatr Int 2019;61:327–38. [CrossRef] google scholar
  • 3. Schroth RJ, Levi JA, Sellers EA, FrielJ, Kliewer E, Moffatt ME. Vitamin D status of children with severe early childhood caries: A case-control study. Bmc Pediatr 2013;13:174. [CrossRef ] google scholar
  • 4. Sheiham A. Oral health, general health and quality of life. Bull World Health Organ 2005;83:644–5. google scholar
  • 5. Selwitz RH, Ismail AI, Pitts NB. Dental caries. Lancet 2007;6:51–9. [CrossRef] google scholar
  • 6. Song E-L, Song C-H, La S-A, Ock S-M, Ju S-Y. Associations of serum vitamin D level with dental caries in Korean adults. Korean J Fam Pract 2016;6:172–8. [CrossRef] google scholar
  • 7. Youssef DA, Miller CWT, El-Abbassi AM, Cutchins DC, Cutchins C, Grant WB, et al. Antimicrobial implications of vitamin D. Dermatoendocrinol 2011;3:220–9. [CrossRef] google scholar
  • 8. Greenstein RJ, Su L, Brown ST. Vitamins A & D inhibit the growth of mycobacteria in radiometric culture. Plos One 2012;7: P.E29631. [CrossRef] google scholar
  • 9. Hosoda K, Shimomura H, Wanibuchi K, Masui H, Amgalanbaatar A, Hayashi S, et al. Identification and characterization of a vitamin D3 decomposition product bactericidal against Helicobacter pylori. Sci Rep 2015;5:8860. [CrossRef] google scholar
  • 10. Grenier D, Morin M, Fournier‐Larente J, Chen H. Vitamin D inhibits the growth of and virulence factor gene expression by Porphyromonas gingivalis and blocks activation of the nuclear factor kappa B transcription factor in monocytes. J Periodontal Res 2016;51:359–65. [CrossRef] google scholar
  • 11. Saputo S, Faustoferri RC, Quivey RG. Vitamin D compounds are bactericidal against Streptococcus mutans and target the bacitracin-associated efflux system. Antimicrob Agents Chemother 2018;62:E01675-17. [CrossRef] google scholar
  • 12. Conrads G, de Soet J, Song L, Henne K, Sztajer H, WagnerDöbler I, et al. Comparing the cariogenic species Streptococcus sobrinus and S. mutans on whole genome level. J Oral Microbiol 2014;6:26189. [CrossRef] google scholar
  • 13. Forssten SD, Björklund M, Ouwehand AC. Streptococcus mutans, caries and simulation models. Nutrients 2010;2:290–8. [CrossRef] google scholar
  • 14. Almoudi MM, Hussein AS, Hassan MIA, Zain NM. A systematic review on antibacterial activity of zinc against Streptococcus mutans. Saudi Dent J 2018;30:283–91. [CrossRef] google scholar
  • 15. Zhu Y, Gasilova N, Jović M, Qiao L, Liu B, Lovey LT, et al. Detection of antimicrobial resistance-associated proteins by titanium dioxide-facilitated intact bacteria mass spectrometry. Chem Sci 2018;9:2212–21. [CrossRef] google scholar
  • 16. DePaola LG, Spolarich AE. Safety and efficacy of antimicrobial mouthrinses in clinical practice. J Dent Hyg. 2007;81:13-25. google scholar
  • 17. McDonnell G, Russell AD. Antiseptics and disinfectants: activity, action, and resistance. Clin Microbiol Rev 1999;12:147–79. [CrossRef] google scholar
  • 18. Fair RJ, Tor Y. Antibiotics and bacterial resistance in the 21st century. Perspect Medicin Chem 2014;6:25-64. [CrossRef] google scholar
  • 19. CLSI. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard—Ninth edition. CLSI document M07-A9. Wayne, PA: Clinical and Laboratory Standards Institute. 2012. google scholar
  • 20. Falcone P, Speranza B, Del Nobile MA, Corbo MR, Sinigaglia M. A study on the antimicrobial activity of thymol intended as a natural preservative. J Food Prot 2005;68:1664–70. [CrossRef ] google scholar
  • 21. AbdElRahman HF, Skaug N. In vitro antimicrobial effects of crude miswak extracts on oral pathogens. In Vitro 2002;10:15–21. google scholar
  • 22. Ramos-Martínez E, López-Vancell MR, de Córdova-Aguirre JCF, Rojas-Serrano J, Chavarría A, Velasco-Medina A, et al. Reduction of respiratory infections in asthma patients supplemented with vitamin D is related to increased serum IL-10 and IFNγ levels and cathelicidin expression. Cytokine 2018;108:239–46. [CrossRef] google scholar
  • 23. Bener A, Ehlayel MS, Bener HZ, Hamid Q. The impact of vitamin D deficiency on asthma, allergic rhinitis and wheezing in children: An emerging public health problem. J Family Community Med 2014;21:154–61. [CrossRef] google scholar
  • 24. Kostoglou-Athanassiou I, Athanassiou P, Lyraki A, Raftakis I, Antoniadis C. Vitamin D and rheumatoid arthritis. Ther Adv Endocrinol Metab 2012;3:181–7. [CrossRef] google scholar
  • 25. Hujoel PP. Vitamin D and dental caries in controlled clinical trials: systematic review and meta-analysis. Nutr Rev 2013;71:88–97. [CrossRef] google scholar
  • 26. Grant WB. A review of the role of solar ultraviolet-B irradiance and vitamin D in reducing risk of dental caries. Dermatoendocrinol 2011;3:193–8. [CrossRef] google scholar
  • 27. Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: A review. J Pharm Anal 2016;6:71–9. [CrossRef] google scholar
  • 28. Weiss EI, Shalhav M, Fuss Z. Assessment of antibacterial activity of endodontic sealers by a direct contact test. Endod Dent Traumatol 1996;12:179–84. [CrossRef] google scholar
  • 29. Rossi A De, Ferreira DCA, Silva RAB da, Queiroz AM de, Silva LAB da, Nelson-Filho P. Antimicrobial activity of toothpastes containing natural extracts, chlorhexidine or triclosan. Braz Dent J 2014;25:186–90. [CrossRef] google scholar
  • 30. Grönroos L, Mättö J, Saarela M, Luoma AR, Luoma H, JousimiesSomer H, et al. Chlorhexidine susceptibilities of mutans streptococcal serotypes and ribotypes. Antimicrob Agents Chemother 1995;39:894–8. [CrossRef] google scholar
  • 31. Azari F, Nyland L, Yu C, Radermacher M, Mintz KP, Ruiz T. Ultrastructural analysis of the rugose cell envelope of a member of the Pasteurellaceae family. J Bacteriol 2013;195:1680–8. [CrossRef] google scholar
  • 32. Martinez de Tejada G, Sánchez-Gómez S, Rázquin-Olazaran I, Kowalski I, Kaconis Y, Heinbockel L, et al. Bacterial cell wall compounds as promising targets of antimicrobial agents I. Antimicrobial peptides and lipopolyamines. Curr Drug Targets 2012;13:1121–30. [CrossRef] google scholar
  • 33. Sinha R, Karan R, Sinha A, Khare SK. Interaction and nanotoxic effect of ZnO and Ag nanoparticles on mesophilic and halophilic bacterial cells. Bioresour Technol 2011;102:1516–20. [CrossRef] google scholar
  • 34. Rahim ZHA, Thurairajah N. Scanning electron microscopic study of Piper betle L. leaves extract effect against Streptococcus mutans ATCC 25175. J Appl Oral Sci 2011;19:137–46. [CrossRef] google scholar
  • 35. Orasmo EAC, Miyakawa W, Otani C, Khouri S. In vitro AFM evaluation of Streptococcus mutans membrane exposed to two mouthwashes. J Appl Pharm Sci 2013;3:24-8. google scholar
  • 36. Ho E-M, Chang H-W, Kim S-I, Kahng H-Y, Oh K-H. Analysis of TNT (2, 4, 6-trinitrotoluene)-inducible cellular responses and stress shock proteome in Stenotrophomonas sp. OK-5. Curr Microbiol 2004;49:346–52. [CrossRef] google scholar
  • 37. Cho Y-S, Oh JJ, Oh K-H. Antimicrobial activity and biofilm formation inhibition of green tea polyphenols on human teeth. Biotechnol Bioprocess Eng 2010;15:359–64. [CrossRef] google scholar

Citations

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APA

Almoudi, M.M., Hussein, A.S., Abu Hassan, M.I., Al Talib, H., Khan, H.B., Nazli, S.A., & Effandy, N.A. (2021). The antibacterial effects of vitamin D3 against mutans streptococci: an in vitro study. European Oral Research, 55(1), 8-15. https://doi.org/10.26650/eor.20210119


AMA

Almoudi M M, Hussein A S, Abu Hassan M I, Al Talib H, Khan H B, Nazli S A, Effandy N A. The antibacterial effects of vitamin D3 against mutans streptococci: an in vitro study. European Oral Research. 2021;55(1):8-15. https://doi.org/10.26650/eor.20210119


ABNT

Almoudi, M.M.; Hussein, A.S.; Abu Hassan, M.I.; Al Talib, H.; Khan, H.B.; Nazli, S.A.; Effandy, N.A. The antibacterial effects of vitamin D3 against mutans streptococci: an in vitro study. European Oral Research, [Publisher Location], v. 55, n. 1, p. 8-15, 2021.


Chicago: Author-Date Style

Almoudi, Manal Mohamed Mansour, and Alaa Sabah Hussein and Mohamed Ibrahim Abu Hassan and Hassanain Al Talib and Hasnah Begum Said Gulam Khan and Siti Arisya Binti Nazli and Nur Aina Efira Binti Effandy. 2021. “The antibacterial effects of vitamin D3 against mutans streptococci: an in vitro study.” European Oral Research 55, no. 1: 8-15. https://doi.org/10.26650/eor.20210119


Chicago: Humanities Style

Almoudi, Manal Mohamed Mansour, and Alaa Sabah Hussein and Mohamed Ibrahim Abu Hassan and Hassanain Al Talib and Hasnah Begum Said Gulam Khan and Siti Arisya Binti Nazli and Nur Aina Efira Binti Effandy. “The antibacterial effects of vitamin D3 against mutans streptococci: an in vitro study.” European Oral Research 55, no. 1 (Apr. 2024): 8-15. https://doi.org/10.26650/eor.20210119


Harvard: Australian Style

Almoudi, MM & Hussein, AS & Abu Hassan, MI & Al Talib, H & Khan, HB & Nazli, SA & Effandy, NA 2021, 'The antibacterial effects of vitamin D3 against mutans streptococci: an in vitro study', European Oral Research, vol. 55, no. 1, pp. 8-15, viewed 20 Apr. 2024, https://doi.org/10.26650/eor.20210119


Harvard: Author-Date Style

Almoudi, M.M. and Hussein, A.S. and Abu Hassan, M.I. and Al Talib, H. and Khan, H.B. and Nazli, S.A. and Effandy, N.A. (2021) ‘The antibacterial effects of vitamin D3 against mutans streptococci: an in vitro study’, European Oral Research, 55(1), pp. 8-15. https://doi.org/10.26650/eor.20210119 (20 Apr. 2024).


MLA

Almoudi, Manal Mohamed Mansour, and Alaa Sabah Hussein and Mohamed Ibrahim Abu Hassan and Hassanain Al Talib and Hasnah Begum Said Gulam Khan and Siti Arisya Binti Nazli and Nur Aina Efira Binti Effandy. “The antibacterial effects of vitamin D3 against mutans streptococci: an in vitro study.” European Oral Research, vol. 55, no. 1, 2021, pp. 8-15. [Database Container], https://doi.org/10.26650/eor.20210119


Vancouver

Almoudi MM, Hussein AS, Abu Hassan MI, Al Talib H, Khan HB, Nazli SA, Effandy NA. The antibacterial effects of vitamin D3 against mutans streptococci: an in vitro study. European Oral Research [Internet]. 20 Apr. 2024 [cited 20 Apr. 2024];55(1):8-15. Available from: https://doi.org/10.26650/eor.20210119 doi: 10.26650/eor.20210119


ISNAD

Almoudi, ManalMohamed Mansour - Hussein, AlaaSabah - Abu Hassan, MohamedIbrahim - Al Talib, Hassanain - Khan, HasnahBegum Said Gulam - Nazli, SitiArisya Binti - Effandy, NurAina Efira Binti. “The antibacterial effects of vitamin D3 against mutans streptococci: an in vitro study”. European Oral Research 55/1 (Apr. 2024): 8-15. https://doi.org/10.26650/eor.20210119



TIMELINE


Submitted15.10.2019
Accepted17.07.2020
Published Online31.01.2021

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