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DOI :10.26650/experimed.2020.823916   IUP :10.26650/experimed.2020.823916    Full Text (PDF)

Cost Evaluation of Fetal Rhesus D Genotyping by Real-time PCR from cell-free Fetal DNA

Büşra Yaşa, Orhan Şahin, Selçuk Sözer Tokdemir

The detection of cell-free fetal DNA (cffDNA) from maternal plasma has enabled the development of essential prenatal diagnostic techniques supporting the non-invasive screening and diagnostic tests in recent years. We performed a non-invasive real-time polymerase chain reaction (PCR) using cell-free DNA isolated from the peripheral blood of pregnant women to determine fetal Rhesus D (RhD) and sex. Consistent with the findings of similar studies, our results revealed high accuracy rates of PCR in determining fetal RhD, making it suitable for diagnostic use, thereby indicating its effectiveness as a guide in treating and especially in minimizing the procedures applied to pregnant women who are RhD negative. This study compared the cost incurred between follow-up testing and treatment of RhD (-) pregnant women and the fetal RHD genotyping based on Health Implementation Notification (SUT) data of the Social Security Administration of the Republic of Turkey. Additionally, the role of PCR to the diagnostic process was evaluated. Our results showed that fetal RHD genotyping costs 523.19 TL (3.5 times) less for each RhD (-) pregnant women compared with the current additional tests and treatments. PCR with cffDNA is an innovative method that minimizes workload, hospital costs, and unnecessary tests and treatment. In addition, this method allows an early initiation of treatment and avoidance of unnecessary intervention and cost.

Keywords: Fetal cell-free DNA, RHD, genotyping, cost analysis
DOI :10.26650/experimed.2020.823916   IUP :10.26650/experimed.2020.823916    Full Text (PDF)

Hücre-dışı Fetal DNA‘dan Gerçek-zamanlı PZR ile Fetal Rhesus D Tespitinin Maliyet Değerlendirmesi

Büşra Yaşa, Orhan Şahin, Selçuk Sözer Tokdemir

Maternal plazmadan hücre-dışı fetal DNA'nın (hdfDNA) tespitinde son yıllarda elde edilen başarılar, hamilelik süresince uygulanabilir, invazif olmayan tarama ve tanı testlerini destekleyen temel tekniklerin gelişmesine olanak sağlamıştır. Yakın zamanda yaptığımız bir çalışmada, invazif işlem gerektirmeden gebelerden alınan periferik kandan hücre-dışı DNA izole edilerek, gerçek zamanlı polimeraz zincir reaksiyonu (PZR) yöntemiyle fetal Rhesus D (RhD) ve cinsiyet belirlenmesi gerçekleştirilmiştir. Bu ve benzeri çalışmalar neticesinde, kullanılan tekniğin fetal RhD tayininde yüksek doğruluk oranlarına sahip olduğu ve tanı amaçlı kullanıma uygun olduğu gösterilmiştir. Tanıda elde edilen bu başarı, özellikle RhD negatif (-) gebelere uygulanan işlemleri en aza indirilerek tedavide yol göstericidir. Bu bağlamda, sunduğumuz bu çalışmada Türkiye Cumhuriyeti, Sosyal Güvenlik Kurumu, Sağlık Uygulama Tebliği (SUT) verileri referans alınarak, günümüzde RhD (-) gebelere uygulanan ek izlem testlerin ve tedavinin oluşturduğu maliyet ile fetal RHD genotiplemesi sonucu ortaya çıkan maliyetin kıyaslaması gerçekleştirilmiştir. Ayrıca, böyle bir yaklaşımın hastalık tanı sürecine yapmış olduğu destek değerlendirilmiştir. Sonuç olarak, her RhD (-) gebe için, gerçek zamanlı PZR uygulanarak fetal RHD genotiplesi ile hali hazırda kullanılan ek tetkik ve tedaviler kıyaslandığında, 523,19 TL (3,5 kat) daha az maliyetli olduğu gösterilmiştir. Bu testin, iş yoğunluğu, hastane maliyetleri ve hastaya uygulanan gereksiz tedavilerin azalması yönünde yenilikçi bir yöntem olduğu görülmektedir. Ayrıca, bu ve benzeri yaklaşımlar, erken tedavinin başlatılması ve gereksiz müdahale ve maliyetten kaçınılmasına olanak sağlayacaktır.

Keywords: Hücre dışı fetal DNA, RHD, genotipleme, maliyet analizi

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APA

Yaşa, B., Şahin, O., & Tokdemir, S.S. (2020). Cost Evaluation of Fetal Rhesus D Genotyping by Real-time PCR from cell-free Fetal DNA. Experimed, 10(3), 124-127. https://doi.org/10.26650/experimed.2020.823916


AMA

Yaşa B, Şahin O, Tokdemir S S. Cost Evaluation of Fetal Rhesus D Genotyping by Real-time PCR from cell-free Fetal DNA. Experimed. 2020;10(3):124-127. https://doi.org/10.26650/experimed.2020.823916


ABNT

Yaşa, B.; Şahin, O.; Tokdemir, S.S. Cost Evaluation of Fetal Rhesus D Genotyping by Real-time PCR from cell-free Fetal DNA. Experimed, [Publisher Location], v. 10, n. 3, p. 124-127, 2020.


Chicago: Author-Date Style

Yaşa, Büşra, and Orhan Şahin and Selçuk Sözer Tokdemir. 2020. “Cost Evaluation of Fetal Rhesus D Genotyping by Real-time PCR from cell-free Fetal DNA.” Experimed 10, no. 3: 124-127. https://doi.org/10.26650/experimed.2020.823916


Chicago: Humanities Style

Yaşa, Büşra, and Orhan Şahin and Selçuk Sözer Tokdemir. “Cost Evaluation of Fetal Rhesus D Genotyping by Real-time PCR from cell-free Fetal DNA.” Experimed 10, no. 3 (Apr. 2024): 124-127. https://doi.org/10.26650/experimed.2020.823916


Harvard: Australian Style

Yaşa, B & Şahin, O & Tokdemir, SS 2020, 'Cost Evaluation of Fetal Rhesus D Genotyping by Real-time PCR from cell-free Fetal DNA', Experimed, vol. 10, no. 3, pp. 124-127, viewed 24 Apr. 2024, https://doi.org/10.26650/experimed.2020.823916


Harvard: Author-Date Style

Yaşa, B. and Şahin, O. and Tokdemir, S.S. (2020) ‘Cost Evaluation of Fetal Rhesus D Genotyping by Real-time PCR from cell-free Fetal DNA’, Experimed, 10(3), pp. 124-127. https://doi.org/10.26650/experimed.2020.823916 (24 Apr. 2024).


MLA

Yaşa, Büşra, and Orhan Şahin and Selçuk Sözer Tokdemir. “Cost Evaluation of Fetal Rhesus D Genotyping by Real-time PCR from cell-free Fetal DNA.” Experimed, vol. 10, no. 3, 2020, pp. 124-127. [Database Container], https://doi.org/10.26650/experimed.2020.823916


Vancouver

Yaşa B, Şahin O, Tokdemir SS. Cost Evaluation of Fetal Rhesus D Genotyping by Real-time PCR from cell-free Fetal DNA. Experimed [Internet]. 24 Apr. 2024 [cited 24 Apr. 2024];10(3):124-127. Available from: https://doi.org/10.26650/experimed.2020.823916 doi: 10.26650/experimed.2020.823916


ISNAD

Yaşa, Büşra - Şahin, Orhan - Tokdemir, SelçukSözer. “Cost Evaluation of Fetal Rhesus D Genotyping by Real-time PCR from cell-free Fetal DNA”. Experimed 10/3 (Apr. 2024): 124-127. https://doi.org/10.26650/experimed.2020.823916



TIMELINE


Submitted10.11.2020
Accepted23.11.2020
Published Online25.12.2020

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