Journal of Istanbul Faculty of Medicine

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Research Article


DOI :10.26650/IUITFD.2019.0023   IUP :10.26650/IUITFD.2019.0023    Full Text (PDF)

THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION

Sacide Pehlivan, Savaş Gürsoy, Ayşe Feyda Nursal, Mazlum Serdar Akaltun, Kürşat Özdilli, Mustafa Pehlivan

Objective: Ankylosing spondylitis (AS) is a chronic inflammatory disorder. MicroRNAs (miRNAs) can function as either oncogenes or tumor suppressor genes. Altered miRNA expression has been implicated in the pathogenesis of several diseases. Therefore, we aimed to explore the effects of miR-196a2T/C (rs11614913) variant profile on susceptibility to AS in a Turkish population.

Materials and Methods: Blood samples were collected from 78 AS patients and 79 healthy controls. miR-196a2T/C variant was genotyped by PCR-RFLP. Odds ratio (OR) with 95% confidence interval (95%CI) were calculated using the χ2 test.

Results: The frequency of T/C and T/T genotypes of the miR196a2T/C were higher in AS patients compared to healthy controls (p=0.034 and p=0.028, respectively). The subjects carrying the miR-196a2T/C variant T/T genotype showed a 2.542-fold increased AS risk than the control group. However, no difference was observed in the allele frequencies of miR-196a2T/C between AS patients and the controls. It was found that C/C genotype of miR-196a2T/C variant was more frequent in AS patients with enthesitis than AS patients without enthesitis (p=0.042).

Conclusion: The miR-196a2T/C variant represents a genetic risk factor for increased susceptibility to AS and is associated with enthesitis in the Turkish population.

Keywords: Ankylosing spondylitis, miR-196a2, variant, enthesis
DOI :10.26650/IUITFD.2019.0023   IUP :10.26650/IUITFD.2019.0023    Full Text (PDF)

TÜRK POPÜLASYONUNDA ANKİLOZAN SPONDİLİTE YATKINLIK SAĞLAYICI FAKTÖR OLARAK miR-196a2T/C VARYANTI

Sacide Pehlivan, Savaş Gürsoy, Ayşe Feyda Nursal, Mazlum Serdar Akaltun, Kürşat Özdilli, Mustafa Pehlivan

Amaç: Ankilozan spondilit (AS) kronik, enflamatuar bir hastalıktır. MikroRNA’lar (miRNA’,lar) onkogen veya tümör baskılayıcı genler olarak fonksiyon yapabilirler. Çeşitli hastalıkların patogenezinde miRNA ekspresyonu’nun değişmesi bulunmaktadır. Bu nedenle, Türk toplumunda AS yatkınlığında miR-196a2T/C (rs11614913) varyant profilinin etkilerini araştırmayı amaçladık.

Gereç ve Yöntem: 78 AS hastası ve 79 kontrolden kan örnekleri toplandı. miR-196a2T/C varyantı PCR-RFLP ile genotiplendi. χ2 testi kullanılarak %95 güven aralığı ile Odds oranı (OR) hesaplandı.

Bulgular: miR-196a2T/C T/C ve T/T genotipleri AS hastalarında sağlıklı kontrollere kıyasla daha yüksekti (sırasıyla, p=0.034 and p=0.028). miR-196a2T/C varyant T/T genotipini taşıyan kişiler kontrol grubundan 2,542 kat artmış AS riski gösterdiler. Ancak, miR-196a2T/C allele frekansları açısından AS hastaları ve kontroller arasında fark saptanmadı. miR-196a2T/C varyant C/C genotipinin entezitli AS hastalarında entezit bulunmayan AS hastalarından daha çok olduğu bulundu (p=0.042).

Sonuç: miR-196a2T/C varyantı Türk toplumunda AS ve hastalığın entezit ile ilişkisine yatkınlık sağlayan genetik risk faktörü rolü göstermektedir.


Keywords: Ankilozan spondilit, miR-196a2, varyant, entezit

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APA

Pehlivan, S., Gürsoy, S., Nursal, A., Akaltun, M., Özdilli, K., & Pehlivan, M. (2020). THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION. Journal of Istanbul Faculty of Medicine, 83(2), 81-85. https://doi.org/10.26650/IUITFD.2019.0023


AMA

Pehlivan S, Gürsoy S, Nursal A, Akaltun M, Özdilli K, Pehlivan M. THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION. Journal of Istanbul Faculty of Medicine. 2020;83(2):81-85. https://doi.org/10.26650/IUITFD.2019.0023


ABNT

Pehlivan, S.; Gürsoy, S.; Nursal, A.; Akaltun, M.; Özdilli, K.; Pehlivan, M. THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION. Journal of Istanbul Faculty of Medicine, [Publisher Location], v. 83, n. 2, p. 81-85, 2020.


Chicago: Author-Date Style

Pehlivan, Sacide, and Savaş Gürsoy and Ayşe Feyda Nursal and Mazlum Serdar Akaltun and Kürşat Özdilli and Mustafa Pehlivan. 2020. “THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION.” Journal of Istanbul Faculty of Medicine 83, no. 2: 81-85. https://doi.org/10.26650/IUITFD.2019.0023


Chicago: Humanities Style

Pehlivan, Sacide, and Savaş Gürsoy and Ayşe Feyda Nursal and Mazlum Serdar Akaltun and Kürşat Özdilli and Mustafa Pehlivan. “THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION.” Journal of Istanbul Faculty of Medicine 83, no. 2 (Apr. 2024): 81-85. https://doi.org/10.26650/IUITFD.2019.0023


Harvard: Australian Style

Pehlivan, S & Gürsoy, S & Nursal, A & Akaltun, M & Özdilli, K & Pehlivan, M 2020, 'THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION', Journal of Istanbul Faculty of Medicine, vol. 83, no. 2, pp. 81-85, viewed 18 Apr. 2024, https://doi.org/10.26650/IUITFD.2019.0023


Harvard: Author-Date Style

Pehlivan, S. and Gürsoy, S. and Nursal, A. and Akaltun, M. and Özdilli, K. and Pehlivan, M. (2020) ‘THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION’, Journal of Istanbul Faculty of Medicine, 83(2), pp. 81-85. https://doi.org/10.26650/IUITFD.2019.0023 (18 Apr. 2024).


MLA

Pehlivan, Sacide, and Savaş Gürsoy and Ayşe Feyda Nursal and Mazlum Serdar Akaltun and Kürşat Özdilli and Mustafa Pehlivan. “THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION.” Journal of Istanbul Faculty of Medicine, vol. 83, no. 2, 2020, pp. 81-85. [Database Container], https://doi.org/10.26650/IUITFD.2019.0023


Vancouver

Pehlivan S, Gürsoy S, Nursal A, Akaltun M, Özdilli K, Pehlivan M. THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION. Journal of Istanbul Faculty of Medicine [Internet]. 18 Apr. 2024 [cited 18 Apr. 2024];83(2):81-85. Available from: https://doi.org/10.26650/IUITFD.2019.0023 doi: 10.26650/IUITFD.2019.0023


ISNAD

Pehlivan, Sacide - Gürsoy, Savaş - Nursal, Ayşe Feyda - Akaltun, Mazlum Serdar - Özdilli, Kürşat - Pehlivan, Mustafa. “THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION”. Journal of Istanbul Faculty of Medicine 83/2 (Apr. 2024): 81-85. https://doi.org/10.26650/IUITFD.2019.0023



TIMELINE


Submitted12.03.2019
Accepted22.08.2019
Published Online24.03.2020

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