Journal of Istanbul Faculty of Medicine

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Research Article


DOI :10.18017/iuitfd.373670   IUP :10.18017/iuitfd.373670    Full Text (PDF)

TİTANYUM DİOKSİT NANOPARÇACIKLARININ YAC-1 LENFOMA HÜCRE SOYU CANLILIĞI VE İNCE YAPISI ÜZERİNE ETKİLERİ

Tuğba Kotil, Aslı Erdoğan, Hasan Serdar Mutlu, Sibel Doğan, Seyhun Solakoğlu

Amaç: Yaygın kullanım alanı olan nanoparçacıkların kanser terapisinde etkili oldukları bilinmektedir. Çalışmamızda geniş kullanım alanına sahip titanyum dioksit nanoparçacıklarının (TiO2-NP) YAC-1 lenfoma hücre soyu üzerine etkilerini incelemeyi amaçladık. 

Gereç ve Yöntem: 24’lü kültür kuyucuklarına ekilen YAC-1 lenfoma hücreleri üzerine iki (0,1mg/mL, 0,5mg/mL) farklı konsantrasyonda TiO2-NP’si uygulandı. 24 ve 48 saat sonra hücreler toplanarak canlılık testi için sayma kamarasında sayım yapıldı ve nicel farklılık Tukey-Kramer çoklu karşılaştırma testi ile belirlendi. Elektron mikroskop incelemesi için rutin takip sonrası Epon-812 ortamına gömülen hücrelerden alınan ince kesitler geçirimli elektron mikroskobu ile incelendi. 

Bulgular: YAC-1 hücrelerinin çoğalma indekslerinde TiO2-NP’lerin dozuna ve uygulama süresine bağlı olarak istatistiksel olarak anlamlı düzeyde azalma izlendi. Elektron mikroskobik incelemede, 24. saatte 0,5mg uygulanmış grupta ve 48. saatte 0,1 ve 0,5 mg uygulanmış grupta mitokondri bozulması, otofajik vakuol birikimi ve hem nekrotik hem de apoptotik hücre morfolojilerine rastlandı. 

Sonuç: TiO2-NP’lerinin farklı kanser hücre soyları üzerinde hücre ölümünü tetiklediği çeşitli çalışmalarla gösterilmiştir. Çalışmamızda hücre çoğalma indeksi açısından doza ve süreye bağlı azalmaya ek olarak elektron mikroskobik olarak nanoparçacığa maruz kalan hücrelerde TiO2-NP içeren otofajik vakuol oluşumları, organellerin yapısında bozulmalar ve apoptotik ve nekrotik hücre ölümünü gösteren morfolojik değişimleri izledik. Çevremizde sürekli etkileşim halinde olduğumuz bu TiO2-NP’sinin hücre üzerinde gösterdiği bu sitotoksik etkinin mekanizmaları, kapsamlı araştırmaların konusu olarak değerlendirilmelidir. 
Keywords: Nanoparçacık, titanyum dioksit, YAC-1, otofaji, apoptoz
DOI :10.18017/iuitfd.373670   IUP :10.18017/iuitfd.373670    Full Text (PDF)

THE EFFECTS OF TITANIUM DIOXIDE NANOPARTICLES ON VITALITY AND ULTRASTRUCTURE OF YAC-1 LYMPHOMA CELL LINE

Tuğba Kotil, Aslı Erdoğan, Hasan Serdar Mutlu, Sibel Doğan, Seyhun Solakoğlu

Objective: Nanoparticles, which are widely used, are known to be effective in cancer therapy. The aim of this study is to investigate the effects of titanium dioxide nanoparticles (TiO2-NP) on the YAC-1 lymphoma cell line. 

Materials and Methods: TiO2-NP were applied at two different concentrations (0.1mg/mL, 0.5mg/ mL) on cultured YAC1 lymphoma cells in 24 well plates. After 24 and 48 hours of treatment the cells were collected and counted for viability test and the quantitative difference was determined by the Tukey-Kramer multiple comparison test. Cells were embedded in Epon-812 after routine process for the electron microscope examination. Thin sections of the cells were examined with Jem Jeol transmission electron microscope.

Results: The proliferation index of YAC-1 cells showed a statistically significant decrease with the dose and time dependent of TiO2-NP treatment. Mitochondrial degeneration, autophagic vacuole accumulation and both necrotic and apoptotic cell morphologies were observed in both TiO2-NP treatment groups via electron microscopic evaluation. 

Conclusion: Several studies have shown that TiO2-NPs trigger cell death on different cancer cell lines. In our study, we observed autophagic vacuole filled with NPs, organelle degeneration, and morphological changes showing apoptotic and necrotic cell death in groups exposed to nanoparticles. In addition significant decrease of proliferation index were detected with dose- and time-dependent. We are constantly interacting with TiO2-NP in our daily day life. The mechanisms of these cytotoxic effects of TiO2-NP should be elucidated by further studies. 

Keywords: Nanoparticles, titanium dioxide; YAC-1; autophagy, apoptosis

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References

  • 1- Bajaj VK, Goyal A, Sharma G, Sharma KB, Gupta RS. Synthesis of CdS Nanoparticle and Reveal Its Effect on Reproductive System of Male Albino Rats. BioNanoSci 2013; 3: 58-66. google scholar
  • 2- Zhang X, Li W, Yang Z, Toxicology of nanosized titanium dioxide: an update. Arch. Toxicol 2015; 89 (12): 2207–2217. google scholar
  • 3- Shi H, Magaye R, Castranova V, Zhao J, Titanium dioxide nanoparticles: a review of current toxicological data. Part. Fibre Toxicol 2013;10:15 google scholar
  • 4- IARC Working Group on the Evaluation of Carcinogenic Risk to Humans. Cobalt in Hard Metals and Cobalt Sulfate, Gallium Arsenide, Indium Phosphide and Vanadium Pentoxide. Lyon (FR): International Agency for Research on Cancer; 2006. (IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, No. 86.). google scholar
  • 5- Bharali DJ, Khalil M, Gurbuz M, Simone TM, Mousa SA. Nanoparticles and cancer therapy: A concise review with emphasis on dendrimers. International Journal of Nanomedicine 2009; 4: 1–7. google scholar
  • 6- Vinardell MP, Mitjans M. Antitumor activities of metal oxide nanoparticles. Nanomaterials 2015; 5: 1004-1021. google scholar
  • 7- Xue C, Wu J, Lan F, Liu W, Yang X, Zeng F, et al. Nano titanium dioxide induces the generation of ROS and potential damage in HaCaT cells under UVA irradiation. J Nanosci Nanotechnol. 2010; 10(12):8500-7. google scholar
  • 8- Thevenot P, Cho J, Wavhal D, Timmons R.B, Tang L. Surface chemistry influence cancer killing effect of TiO2 nanoparticles. Nanomedicine, 2008;4(3): 226-236. google scholar
  • 9- Vamanu CI, Cimpan MR, Hol PJ, Sornes S, Lie SA, Gjerdet NR. Induction of cell death by TiO2 nanoparticles: Studies on a human monoblastoid cell line. Toxicology in Vitro 2008; 22: 1689-1896. google scholar
  • 10- Zhu Y, Eaton JW, Li C. Titanium dioxide (TiO2) nanoparticles preferentially induce cell death in transformed cell in a Bak/Bax- independent fashion. PLoSONE 2012; 7(11): 1-11. google scholar
  • 11- Luo YH, Chang LW, Lin P. Metal-Based Nanoparticles and the Immune System: Activation, Inflammation, and Potential Applications. BioMed Research International 2015;1-12. google scholar
  • 12- Thurn KT, Arora H, Paunesku T, Wu A, Brown EMB, Doty C, Kremer J, Woloschak G. Endocytosis of Titanium Dioxide Nanoparticles in Prostate Cancer PC-3M Cells. Nanomedicine 2011;7(2): 123-130. google scholar
  • 13- Sarhan OMM, Hussein RM. Effects of intraperitoneally injected silver nanoparticles on histological structures and blood parameters in the albino rat. International Journal of Nanomedicine 2014; 9: 1505-1517. google scholar
  • 14- Lopes VR, Vesa Loitto V, Audinot JN, Bayat N, Gutleb AC, Cristobal S. Dose dependent autophagic effect of titanium dioxide nanoparticles in human HaCaT cells at non cytotoxic levels. J Nanobiotechnol 2016; 14: 22. google scholar
  • 15- Halamoda KB, Chapuıs BC, Guney-Ayra S, Juillerat-Jeanneret L. Induction of oxidative stress, lysosome activation and autophagy by nanoparticles in human brain-derived endothelial cells. Biochem 2012; 441: 813-821. google scholar
  • 16- Chen Y, Azad MB, Gibson SB. Superoxide is the major reactive oxygen species regulating autophagy. Cell Death and Differentiation 2009; 16: 1040–1052. google scholar
  • 17- Levine B, Yuan J. Autophagy in cell death: an innocent convict? J Clin Invest 2005; 115:2679–2688 google scholar
  • 18- Glick D, Barth S, Macleod KF. Autophagy: cellular and molecular mechanisms. The Journal of pathology. 2010; 221(1):3-12. google scholar
  • 19- Krysko DV, Kaczmarek A, Vandenabeele P. Molecular Pathways of Different Types of Cell Death: Many Roads to Death. Phagocytosis of Dying Cells: From Molecular Mechanisms to Human Diseases; 2009.p.3-31. google scholar
  • 20- Azad MB, Chen Y, Gibson SB. Regulaton of autophagy by reactive oxygen species (ROS): implications for cancer pregression and treatment. Antioxid Redox Signal 2009; 11(4): 777-790. google scholar
  • 21-Wang Y, Cui H, Zhou J, Li F, Wang J, Chen M, et al. Cytotoxicity, DNA damage, and apoptosis induced by titanium dioxide nanoparticles in human non-small cell lung cancer A549 cells. Environ Sci Pollut Res Int. 2015; 22(7): 5519-30. google scholar
  • 22- Parka EJ, Yib J, Chungc KH, Ryud DY, Choie J, Park K. Oxidative stress and apoptosis induced by titanium dioxide nanoparticles in cultured BEAS-2B cells Toxicology Letters 2008;180: 222–229. google scholar
  • 23- Li XB, Xu SQ, Zhang ZR, Schluesener HJ. Apoptosis induced by titanium dioxide nanoparticles in cultured murine microglia N9 cells. Chinese Sci Bull 2009; 54: 3830―3836. google scholar
  • 24- Zhao J, Bowman L, Zhang X, Vallyathan V, Young SH, Castranova V, et al. Titanium dioxide (TiO2) nanoparticles induce JB6 cell apoptosis through activation of the caspase-8/Bid and mitochondrial pathways. J Toxicol Environ Health A. 2009;72(19):1141-9. google scholar

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APA

Kotil, T., Erdoğan, A., Mutlu, H., Doğan, S., & Solakoğlu, S. (2018). TİTANYUM DİOKSİT NANOPARÇACIKLARININ YAC-1 LENFOMA HÜCRE SOYU CANLILIĞI VE İNCE YAPISI ÜZERİNE ETKİLERİ. Journal of Istanbul Faculty of Medicine, 81(1), 17-24. https://doi.org/10.18017/iuitfd.373670


AMA

Kotil T, Erdoğan A, Mutlu H, Doğan S, Solakoğlu S. TİTANYUM DİOKSİT NANOPARÇACIKLARININ YAC-1 LENFOMA HÜCRE SOYU CANLILIĞI VE İNCE YAPISI ÜZERİNE ETKİLERİ. Journal of Istanbul Faculty of Medicine. 2018;81(1):17-24. https://doi.org/10.18017/iuitfd.373670


ABNT

Kotil, T.; Erdoğan, A.; Mutlu, H.; Doğan, S.; Solakoğlu, S. TİTANYUM DİOKSİT NANOPARÇACIKLARININ YAC-1 LENFOMA HÜCRE SOYU CANLILIĞI VE İNCE YAPISI ÜZERİNE ETKİLERİ. Journal of Istanbul Faculty of Medicine, [Publisher Location], v. 81, n. 1, p. 17-24, 2018.


Chicago: Author-Date Style

Kotil, Tuğba, and Aslı Erdoğan and Hasan Serdar Mutlu and Sibel Doğan and Seyhun Solakoğlu. 2018. “TİTANYUM DİOKSİT NANOPARÇACIKLARININ YAC-1 LENFOMA HÜCRE SOYU CANLILIĞI VE İNCE YAPISI ÜZERİNE ETKİLERİ.” Journal of Istanbul Faculty of Medicine 81, no. 1: 17-24. https://doi.org/10.18017/iuitfd.373670


Chicago: Humanities Style

Kotil, Tuğba, and Aslı Erdoğan and Hasan Serdar Mutlu and Sibel Doğan and Seyhun Solakoğlu. “TİTANYUM DİOKSİT NANOPARÇACIKLARININ YAC-1 LENFOMA HÜCRE SOYU CANLILIĞI VE İNCE YAPISI ÜZERİNE ETKİLERİ.” Journal of Istanbul Faculty of Medicine 81, no. 1 (May. 2025): 17-24. https://doi.org/10.18017/iuitfd.373670


Harvard: Australian Style

Kotil, T & Erdoğan, A & Mutlu, H & Doğan, S & Solakoğlu, S 2018, 'TİTANYUM DİOKSİT NANOPARÇACIKLARININ YAC-1 LENFOMA HÜCRE SOYU CANLILIĞI VE İNCE YAPISI ÜZERİNE ETKİLERİ', Journal of Istanbul Faculty of Medicine, vol. 81, no. 1, pp. 17-24, viewed 15 May. 2025, https://doi.org/10.18017/iuitfd.373670


Harvard: Author-Date Style

Kotil, T. and Erdoğan, A. and Mutlu, H. and Doğan, S. and Solakoğlu, S. (2018) ‘TİTANYUM DİOKSİT NANOPARÇACIKLARININ YAC-1 LENFOMA HÜCRE SOYU CANLILIĞI VE İNCE YAPISI ÜZERİNE ETKİLERİ’, Journal of Istanbul Faculty of Medicine, 81(1), pp. 17-24. https://doi.org/10.18017/iuitfd.373670 (15 May. 2025).


MLA

Kotil, Tuğba, and Aslı Erdoğan and Hasan Serdar Mutlu and Sibel Doğan and Seyhun Solakoğlu. “TİTANYUM DİOKSİT NANOPARÇACIKLARININ YAC-1 LENFOMA HÜCRE SOYU CANLILIĞI VE İNCE YAPISI ÜZERİNE ETKİLERİ.” Journal of Istanbul Faculty of Medicine, vol. 81, no. 1, 2018, pp. 17-24. [Database Container], https://doi.org/10.18017/iuitfd.373670


Vancouver

Kotil T, Erdoğan A, Mutlu H, Doğan S, Solakoğlu S. TİTANYUM DİOKSİT NANOPARÇACIKLARININ YAC-1 LENFOMA HÜCRE SOYU CANLILIĞI VE İNCE YAPISI ÜZERİNE ETKİLERİ. Journal of Istanbul Faculty of Medicine [Internet]. 15 May. 2025 [cited 15 May. 2025];81(1):17-24. Available from: https://doi.org/10.18017/iuitfd.373670 doi: 10.18017/iuitfd.373670


ISNAD

Kotil, Tuğba - Erdoğan, Aslı - Mutlu, Hasan Serdar - Doğan, Sibel - Solakoğlu, Seyhun. “TİTANYUM DİOKSİT NANOPARÇACIKLARININ YAC-1 LENFOMA HÜCRE SOYU CANLILIĞI VE İNCE YAPISI ÜZERİNE ETKİLERİ”. Journal of Istanbul Faculty of Medicine 81/1 (May. 2025): 17-24. https://doi.org/10.18017/iuitfd.373670



TIMELINE


Submitted02.01.2018
Accepted07.02.2018
Published Online15.03.2018

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