Research Article

DOI :10.26650/EurJBiol.2023.1239283   IUP :10.26650/EurJBiol.2023.1239283    Full Text (PDF)

Anti-Bacterial, Anti-Mycobacterial and Anti-Fungal Properties of Punica granatum as Natural Dye

Pınar GünerTülin Aşkun

Objective: This study aimed to determine the anti-mycobacterial, anti-bacterial, and anti-fungal effects of dry/fresh pomegranate peel ethanol/methanol extracts, and the dyeing performance and antimicrobial effects of dyed fabric samples with pomegranate peel ethanol extract.

Materials and Methods: Anti-mycobacterial activity against Mycobacterium tuberculosis H37Ra/H37Rv and two-clinical M. tuberculosis strains, and anti-bacterial activity against eight bacteria (Bacillus cereus, Staphylococcus aureus, Salmonella typhimurium, Pseudomonas aeruginosa, Klebsiella pneumonia, Proteus vulgaris, Methicillin-resistant Staphylococcus aureus and Escherichia coli) and anti-fungal activity against five fungal pathogens (Candida albicans, Aspergillus flavus, Aspergillus ochraceus, Aspergillus niger, Fusarium proliferatum) were determined by microplate assay. The anti-microbial activity of dyed fabric samples (30/ 1 Rib and single jersey 100% cotton) as well as their coloring properties, were investigated using the parallel streak method (AATCC 147).

Results: Extracts showed the anti-mycobacterial efficacy between MIC 7.81-31.25 𝜇g/ml and MBC 31.25-250 𝜇g/ml, respectively against four strains of M. tuberculosis. Also, each extract showed anti-bacterial activity between MIC 0.97-62.50 𝜇g/ml and MBC 7.81-250 𝜇g/ml and anti-fungal activity between MIC 31.25-125 𝜇g/ml and MBC 125-250 𝜇g/ml. While control and mordanting of fabric samples did not show any inhibition zones, significant anti-microbial activity against S. aureus was obtained after dyeing using dry peel on fabric samples without mordant.

Conclusion: These findings provide valuable information for future applications of natural dyes in textiles. The anti-mycobacterial, anti-fungal, and anti-bacterial properties of pomegranate could be significant in developing a model for drug design. 

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Güner, P., & Aşkun, T. (2023). Anti-Bacterial, Anti-Mycobacterial and Anti-Fungal Properties of Punica granatum as Natural Dye. European Journal of Biology, 82(1), 38-48.


Güner P, Aşkun T. Anti-Bacterial, Anti-Mycobacterial and Anti-Fungal Properties of Punica granatum as Natural Dye. European Journal of Biology. 2023;82(1):38-48.


Güner, P.; Aşkun, T. Anti-Bacterial, Anti-Mycobacterial and Anti-Fungal Properties of Punica granatum as Natural Dye. European Journal of Biology, [Publisher Location], v. 82, n. 1, p. 38-48, 2023.

Chicago: Author-Date Style

Güner, Pınar, and Tülin Aşkun. 2023. “Anti-Bacterial, Anti-Mycobacterial and Anti-Fungal Properties of Punica granatum as Natural Dye.” European Journal of Biology 82, no. 1: 38-48.

Chicago: Humanities Style

Güner, Pınar, and Tülin Aşkun. Anti-Bacterial, Anti-Mycobacterial and Anti-Fungal Properties of Punica granatum as Natural Dye.” European Journal of Biology 82, no. 1 (Mar. 2024): 38-48.

Harvard: Australian Style

Güner, P & Aşkun, T 2023, 'Anti-Bacterial, Anti-Mycobacterial and Anti-Fungal Properties of Punica granatum as Natural Dye', European Journal of Biology, vol. 82, no. 1, pp. 38-48, viewed 3 Mar. 2024,

Harvard: Author-Date Style

Güner, P. and Aşkun, T. (2023) ‘Anti-Bacterial, Anti-Mycobacterial and Anti-Fungal Properties of Punica granatum as Natural Dye’, European Journal of Biology, 82(1), pp. 38-48. (3 Mar. 2024).


Güner, Pınar, and Tülin Aşkun. Anti-Bacterial, Anti-Mycobacterial and Anti-Fungal Properties of Punica granatum as Natural Dye.” European Journal of Biology, vol. 82, no. 1, 2023, pp. 38-48. [Database Container],


Güner P, Aşkun T. Anti-Bacterial, Anti-Mycobacterial and Anti-Fungal Properties of Punica granatum as Natural Dye. European Journal of Biology [Internet]. 3 Mar. 2024 [cited 3 Mar. 2024];82(1):38-48. Available from: doi: 10.26650/EurJBiol.2023.1239283


Güner, Pınar - Aşkun, Tülin. Anti-Bacterial, Anti-Mycobacterial and Anti-Fungal Properties of Punica granatum as Natural Dye”. European Journal of Biology 82/1 (Mar. 2024): 38-48.


Published Online26.06.2023


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