Original Research


DOI :10.26650/eor.20210119   IUP :10.26650/eor.20210119    Full Text (PDF)

The antibacterial effects of vitamin D3 against mutans streptococci: an in vitro study

Manal Mohamed Mansour AlmoudiAlaa Sabah HusseinMohamed Ibrahim Abu HassanHassanain Al TalibHasnah Begum Said Gulam KhanSiti Arisya Binti NazliNur Aina Efira Binti Effandy

Purpose This study aims to evaluate the antimicrobial effects of the cholecalciferol vitamin D3 against Streptococcus sobrinus (Strep. sobrinus) and Streptococcus mutans (Strep. mutans) bacteria in vitro that is considered the main causative bacteria in dental caries development. Materials and Methods The antimicrobial effects of vitamin D3 were evaluated against Strep. sobrinus and Strep mutans using the agar disc diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of vitamin D3 were determined using a microdilution method following the guidelines by the Clinical Laboratory Standards Institute (CLSI). Scanning electron microscope (SEM) was used to evaluate the morphological changes of bacterial cells following exposure to vitamin D3. Results Strep. sobrinus was more sensitive to vitamin D3 compared to Strep. mutans bacteria. The MIC values of vitamin D3 against Strep. sobrinus and Strep. mutans were 60 µg/ mL and 250 µg/mL respectively whereas the MBC values were 120 µg/mL and 500 µg/mL, respectively. Moreover, significant changes in the bacterial morphology were observed in treated bacterial cells with vitamin D3 as compared to the untreated control bacteria using SEM. Conclusion These findings suggested that vitamin D3 has excellent antimicrobial effects against Strep. sobrinus and Strep. mutans and may be considered as a promising compound in the prevention of dental caries in the future. Further research is recommended to elucidate the mechanism of vitamin D3 on these bacteria.


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APA

Almoudi, M.M., Hussein, A.S., Abu Hassan, M.I., Al Talib, H., Khan, H.B., Nazli, S.A., & Effandy, N.A. (2021). The antibacterial effects of vitamin D3 against mutans streptococci: an in vitro study. European Oral Research, 55(1), 8-15. https://doi.org/10.26650/eor.20210119


AMA

Almoudi M M, Hussein A S, Abu Hassan M I, Al Talib H, Khan H B, Nazli S A, Effandy N A. The antibacterial effects of vitamin D3 against mutans streptococci: an in vitro study. European Oral Research. 2021;55(1):8-15. https://doi.org/10.26650/eor.20210119


ABNT

Almoudi, M.M.; Hussein, A.S.; Abu Hassan, M.I.; Al Talib, H.; Khan, H.B.; Nazli, S.A.; Effandy, N.A. The antibacterial effects of vitamin D3 against mutans streptococci: an in vitro study. European Oral Research, [Publisher Location], v. 55, n. 1, p. 8-15, 2021.


Chicago: Author-Date Style

Almoudi, Manal Mohamed Mansour, and Alaa Sabah Hussein and Mohamed Ibrahim Abu Hassan and Hassanain Al Talib and Hasnah Begum Said Gulam Khan and Siti Arisya Binti Nazli and Nur Aina Efira Binti Effandy. 2021. “The antibacterial effects of vitamin D3 against mutans streptococci: an in vitro study.” European Oral Research 55, no. 1: 8-15. https://doi.org/10.26650/eor.20210119


Chicago: Humanities Style

Almoudi, Manal Mohamed Mansour, and Alaa Sabah Hussein and Mohamed Ibrahim Abu Hassan and Hassanain Al Talib and Hasnah Begum Said Gulam Khan and Siti Arisya Binti Nazli and Nur Aina Efira Binti Effandy. The antibacterial effects of vitamin D3 against mutans streptococci: an in vitro study.” European Oral Research 55, no. 1 (Feb. 2021): 8-15. https://doi.org/10.26650/eor.20210119


Harvard: Australian Style

Almoudi, MM & Hussein, AS & Abu Hassan, MI & Al Talib, H & Khan, HB & Nazli, SA & Effandy, NA 2021, 'The antibacterial effects of vitamin D3 against mutans streptococci: an in vitro study', European Oral Research, vol. 55, no. 1, pp. 8-15, viewed 27 Feb. 2021, https://doi.org/10.26650/eor.20210119


Harvard: Author-Date Style

Almoudi, M.M. and Hussein, A.S. and Abu Hassan, M.I. and Al Talib, H. and Khan, H.B. and Nazli, S.A. and Effandy, N.A. (2021) ‘The antibacterial effects of vitamin D3 against mutans streptococci: an in vitro study’, European Oral Research, 55(1), pp. 8-15. https://doi.org/10.26650/eor.20210119 (27 Feb. 2021).


MLA

Almoudi, Manal Mohamed Mansour, and Alaa Sabah Hussein and Mohamed Ibrahim Abu Hassan and Hassanain Al Talib and Hasnah Begum Said Gulam Khan and Siti Arisya Binti Nazli and Nur Aina Efira Binti Effandy. The antibacterial effects of vitamin D3 against mutans streptococci: an in vitro study.” European Oral Research, vol. 55, no. 1, 2021, pp. 8-15. [Database Container], https://doi.org/10.26650/eor.20210119


Vancouver

Almoudi MM, Hussein AS, Abu Hassan MI, Al Talib H, Khan HB, Nazli SA, Effandy NA. The antibacterial effects of vitamin D3 against mutans streptococci: an in vitro study. European Oral Research [Internet]. 27 Feb. 2021 [cited 27 Feb. 2021];55(1):8-15. Available from: https://doi.org/10.26650/eor.20210119 doi: 10.26650/eor.20210119


ISNAD

Almoudi, ManalMohamed Mansour - Hussein, AlaaSabah - Abu Hassan, MohamedIbrahim - Al Talib, Hassanain - Khan, HasnahBegum Said Gulam - Nazli, SitiArisya Binti - Effandy, NurAina Efira Binti. The antibacterial effects of vitamin D3 against mutans streptococci: an in vitro study”. European Oral Research 55/1 (Feb. 2021): 8-15. https://doi.org/10.26650/eor.20210119



TIMELINE


Submitted15.10.2019
Accepted17.07.2020
Published Online31.01.2021

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