Research Article


DOI :10.26650/eor.20241459780   IUP :10.26650/eor.20241459780    Full Text (PDF)

The antimicrobial effect of R-limonene and its nano emulsion on Enterococcus faecalis - In vitro study

İlke Doğa ŞekerTayfun AlaçamGülçin AkçaAysel YılmazSevgi Takka

Purpose: This study aimed to evaluate the effectiveness of R-limonene and its nano emulsion formulation against Enterococcus faecalis (E. faecalis) in infected root canals.

Materials and Methods: A nano emulsion containing 20% R-limonene was prepared using the phase inversion method. E. faecalis was cultivated, and MIC/MBC values were determined through the macrodilution method. Standardized E. faecalis suspensions were used to infect the root canals ex vivo. Subsequently, R-limonene, nano emulsion containing 20% R-limonene, calcium hydroxide, and sterile saline were applied to the infected root canals for 7 days at the determined MIC/MBC dose. The root canals were sampled, and droplets were plated onto Mueller-Hinton agar plates, and viable bacteria (Colony Forming Units, CFU/mL) were counted. Statistical analyzes were performed based on the data.

Results: Statistically similar efficacy was observed between test materials on the root canal samples. All test materials were significantly more effective than the saline control (p<0.05), however, none of the test materials completely eliminated E. faecalis from root canals.

Conclusion: Although the effects of R-limonene on E. faecalis in infected root canals appeared to be like calcium hydroxide, with its broad antibacterial spectrum, gutta-percha softening and cleansing benefits, it was considered that it could considered among antimicrobials that could be used especially in endodontic retreatments alone or in synergistic combinations.


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APA

Şeker, İ.D., Alaçam, T., Akça, G., Yılmaz, A., & Takka, S. (2024). The antimicrobial effect of R-limonene and its nano emulsion on Enterococcus faecalis - In vitro study. European Oral Research, 0(0), -. https://doi.org/10.26650/eor.20241459780


AMA

Şeker İ D, Alaçam T, Akça G, Yılmaz A, Takka S. The antimicrobial effect of R-limonene and its nano emulsion on Enterococcus faecalis - In vitro study. European Oral Research. 2024;0(0):-. https://doi.org/10.26650/eor.20241459780


ABNT

Şeker, İ.D.; Alaçam, T.; Akça, G.; Yılmaz, A.; Takka, S. The antimicrobial effect of R-limonene and its nano emulsion on Enterococcus faecalis - In vitro study. European Oral Research, [Publisher Location], v. 0, n. 0, p. -, 2024.


Chicago: Author-Date Style

Şeker, İlke Doğa, and Tayfun Alaçam and Gülçin Akça and Aysel Yılmaz and Sevgi Takka. 2024. “The antimicrobial effect of R-limonene and its nano emulsion on Enterococcus faecalis - In vitro study.” European Oral Research 0, no. 0: -. https://doi.org/10.26650/eor.20241459780


Chicago: Humanities Style

Şeker, İlke Doğa, and Tayfun Alaçam and Gülçin Akça and Aysel Yılmaz and Sevgi Takka. The antimicrobial effect of R-limonene and its nano emulsion on Enterococcus faecalis - In vitro study.” European Oral Research 0, no. 0 (Oct. 2024): -. https://doi.org/10.26650/eor.20241459780


Harvard: Australian Style

Şeker, İD & Alaçam, T & Akça, G & Yılmaz, A & Takka, S 2024, 'The antimicrobial effect of R-limonene and its nano emulsion on Enterococcus faecalis - In vitro study', European Oral Research, vol. 0, no. 0, pp. -, viewed 11 Oct. 2024, https://doi.org/10.26650/eor.20241459780


Harvard: Author-Date Style

Şeker, İ.D. and Alaçam, T. and Akça, G. and Yılmaz, A. and Takka, S. (2024) ‘The antimicrobial effect of R-limonene and its nano emulsion on Enterococcus faecalis - In vitro study’, European Oral Research, 0(0), pp. -. https://doi.org/10.26650/eor.20241459780 (11 Oct. 2024).


MLA

Şeker, İlke Doğa, and Tayfun Alaçam and Gülçin Akça and Aysel Yılmaz and Sevgi Takka. The antimicrobial effect of R-limonene and its nano emulsion on Enterococcus faecalis - In vitro study.” European Oral Research, vol. 0, no. 0, 2024, pp. -. [Database Container], https://doi.org/10.26650/eor.20241459780


Vancouver

Şeker İD, Alaçam T, Akça G, Yılmaz A, Takka S. The antimicrobial effect of R-limonene and its nano emulsion on Enterococcus faecalis - In vitro study. European Oral Research [Internet]. 11 Oct. 2024 [cited 11 Oct. 2024];0(0):-. Available from: https://doi.org/10.26650/eor.20241459780 doi: 10.26650/eor.20241459780


ISNAD

Şeker, İlkeDoğa - Alaçam, Tayfun - Akça, Gülçin - Yılmaz, Aysel - Takka, Sevgi. The antimicrobial effect of R-limonene and its nano emulsion on Enterococcus faecalis - In vitro study”. European Oral Research 0/0 (Oct. 2024): -. https://doi.org/10.26650/eor.20241459780



TIMELINE


Submitted27.03.2024
Accepted16.05.2024
Published Online25.07.2024

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