Research Article


DOI :10.26650/experimed.2021.874159   IUP :10.26650/experimed.2021.874159    Full Text (PDF)

A Brief Reconnoitre about Effects of MMP9 on Aortic Dissection

Burcu Salman YaylazMelda SerimanAhmet EkmekçiEmel ErgülMahmut UluganyanFulya CoşanÖzgün Melike Gedar TotukNeslihan Abacı

Objective: Matrix metalloproteinases (MMPs) are the extracellular matrix regulators that frequently investigate cardiovascular diseases and cancer metastasis. Our study aimed to examine specific polymorphisms in the MMP9 gene in our patients with aortic dissection and compare the effect of MMP9 on aortic dissection with expression datasets. Materials and Methods: Q279R and P574R polymorphisms were analyzed in 44 aortic dissection patients and 40 healthy donors via polymerase chain reaction-restriction fragment length polymorphism. (PCR-RFLP) methods. Q279R and P574R prevalence was statistically compared with the medical data of the patients. Additionally, we collected datasets of aortic dissection from NCBI GEO to reanalyze GEO2R and RStudio to see metalloproteinase activity on samples. Later, enrichment analysis was processed on widely used databases. Results: Genotypic distribution of alleles was similar in the two study groups. In addition to this, female CG carriers had a higher risk of developing aortic dissection than those of males. As the results of the protein-protein interaction analysis of MMP9 and patients’ clinical data, hypertension was found to be the significant outcome of P574R variation in the patients. In array analysis, MMP9 expression did not change critically, but TIMPs had been downregulated in many samples. Also, MMP9 targeted miRNA expression levels were detected as low in aortic tissue and blood. Conclusion: Q279R and P574R are two polymorphisms that do not directly affect MMP9 protein structure. Consequently, studied polymorphisms and performed meta-analysis show that MMP9 does not spark off the phenotype but sets the stage for aortic dissection development as seen in the statistical results. Furthermore, enrichment analysis on datasets shows MMP9 was not a primary reason for vascular remodeling.

DOI :10.26650/experimed.2021.874159   IUP :10.26650/experimed.2021.874159    Full Text (PDF)

MMP9 Geninin Aort Diseksiyonundaki Olası Etkilerinin Araştırılması

Burcu Salman YaylazMelda SerimanAhmet EkmekçiEmel ErgülMahmut UluganyanFulya CoşanÖzgün Melike Gedar TotukNeslihan Abacı

Amaç: Kardiyovasküler hastalıklar ve kanser metastazında sıklıkla araştırılan matriks metalloproteinazlar (MMPs) ekstraselüler matriks düzenleyicileridir. Çalışmamız, aort diseksiyonu olan hastalarda MMP9 genindeki spesifik polimorfizmleri incelemeyi ve MMP9'un aort diseksiyonu üzerindeki etkisini ekspresyon veri setleri ile karşılaştırmayı amaçlamaktadır. Gereç ve Yöntem: Q279R ve P574R polimorfizmleri 44 aort diseksiyon tanısı almış ve 40 sağlıklı bireyde polimeraz zincir reaksiyonu - restriksiyon parça uzunluğu polimorfizmi (PCR-RFLP) yöntemiyle çalışıldı. Q279R ve P574R prevalansı istatistiksel olarak hastaların tıbbi verileriyle karşılaştırıldı. Buna ek olarak, NCBI GEO veri tabanından aort diseksiyon veri setleri toplandı ve MMP9 ifadesindeki farklılıkları görmek amacıyla bu veri setleri GEO2R ve RStudio ile yeniden analiz edildi. Elde edilen sonuçların informatik analizi için çevrimiçi veri tabanları kullanıldı. Bulgular: CG alleli taşıyıcısı kadınların aort diseksiyonu geliştirme riski erkeklerden daha yüksek bulunmasına rağmen her iki çalışma grubunda da allellerin genotipik dağılımı benzer bulunmuştur. MMP9'un protein-protein etkileşim analizinin ve hastaların tıbbi verilerinin incelenmesinin sonucu olarak, P574R hipertansiyonu olan hastalarda önemli bir bulgu olarak değerlendirilmiştir. Array verisi analizinde ise MMP9 ifadesinde kritik bir değişim gözlemlenmemiş olup, birçok örnekte TIMP ifade seviyelerinde azalma tespit edilmiştir. Ayrıca MMP9’u hedefleyen miRNA ekspresyon seviyelerinin aort dokusu ve kanda düşük olduğu saptanmıştır. Sonuç: Q279R ve P574R, MMP9 protein yapısını doğrudan etkilemeyen iki polimorfizmdir. İncelenen polimorfizmler ve gerçekleştirilen meta-analizler, MMP9'un fenotipi doğrudan etkilemediği, ancak istatistiksel sonuçlarda görüldüğü gibi aort diseksiyonu gelişimi için zemin hazırladığını göstermektedir.


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APA

Salman Yaylaz, B., Seriman, M., Ekmekçi, A., Ergül, E., Uluganyan, M., Coşan, F., Gedar Totuk, Ö.M., & Abacı, N. (2021). A Brief Reconnoitre about Effects of MMP9 on Aortic Dissection. Experimed, 11(1), 12-20. https://doi.org/10.26650/experimed.2021.874159


AMA

Salman Yaylaz B, Seriman M, Ekmekçi A, Ergül E, Uluganyan M, Coşan F, Gedar Totuk Ö M, Abacı N. A Brief Reconnoitre about Effects of MMP9 on Aortic Dissection. Experimed. 2021;11(1):12-20. https://doi.org/10.26650/experimed.2021.874159


ABNT

Salman Yaylaz, B.; Seriman, M.; Ekmekçi, A.; Ergül, E.; Uluganyan, M.; Coşan, F.; Gedar Totuk, Ö.M.; Abacı, N. A Brief Reconnoitre about Effects of MMP9 on Aortic Dissection. Experimed, [Publisher Location], v. 11, n. 1, p. 12-20, 2021.


Chicago: Author-Date Style

Salman Yaylaz, Burcu, and Melda Seriman and Ahmet Ekmekçi and Emel Ergül and Mahmut Uluganyan and Fulya Coşan and Özgün Melike Gedar Totuk and Neslihan Abacı. 2021. “A Brief Reconnoitre about Effects of MMP9 on Aortic Dissection.” Experimed 11, no. 1: 12-20. https://doi.org/10.26650/experimed.2021.874159


Chicago: Humanities Style

Salman Yaylaz, Burcu, and Melda Seriman and Ahmet Ekmekçi and Emel Ergül and Mahmut Uluganyan and Fulya Coşan and Özgün Melike Gedar Totuk and Neslihan Abacı. A Brief Reconnoitre about Effects of MMP9 on Aortic Dissection.” Experimed 11, no. 1 (Jul. 2021): 12-20. https://doi.org/10.26650/experimed.2021.874159


Harvard: Australian Style

Salman Yaylaz, B & Seriman, M & Ekmekçi, A & Ergül, E & Uluganyan, M & Coşan, F & Gedar Totuk, ÖM & Abacı, N 2021, 'A Brief Reconnoitre about Effects of MMP9 on Aortic Dissection', Experimed, vol. 11, no. 1, pp. 12-20, viewed 25 Jul. 2021, https://doi.org/10.26650/experimed.2021.874159


Harvard: Author-Date Style

Salman Yaylaz, B. and Seriman, M. and Ekmekçi, A. and Ergül, E. and Uluganyan, M. and Coşan, F. and Gedar Totuk, Ö.M. and Abacı, N. (2021) ‘A Brief Reconnoitre about Effects of MMP9 on Aortic Dissection’, Experimed, 11(1), pp. 12-20. https://doi.org/10.26650/experimed.2021.874159 (25 Jul. 2021).


MLA

Salman Yaylaz, Burcu, and Melda Seriman and Ahmet Ekmekçi and Emel Ergül and Mahmut Uluganyan and Fulya Coşan and Özgün Melike Gedar Totuk and Neslihan Abacı. A Brief Reconnoitre about Effects of MMP9 on Aortic Dissection.” Experimed, vol. 11, no. 1, 2021, pp. 12-20. [Database Container], https://doi.org/10.26650/experimed.2021.874159


Vancouver

Salman Yaylaz B, Seriman M, Ekmekçi A, Ergül E, Uluganyan M, Coşan F, Gedar Totuk ÖM, Abacı N. A Brief Reconnoitre about Effects of MMP9 on Aortic Dissection. Experimed [Internet]. 25 Jul. 2021 [cited 25 Jul. 2021];11(1):12-20. Available from: https://doi.org/10.26650/experimed.2021.874159 doi: 10.26650/experimed.2021.874159


ISNAD

Salman Yaylaz, Burcu - Seriman, Melda - Ekmekçi, Ahmet - Ergül, Emel - Uluganyan, Mahmut - Coşan, Fulya - Gedar Totuk, ÖzgünMelike - Abacı, Neslihan. A Brief Reconnoitre about Effects of MMP9 on Aortic Dissection”. Experimed 11/1 (Jul. 2021): 12-20. https://doi.org/10.26650/experimed.2021.874159



TIMELINE


Submitted09.02.2021
Accepted21.03.2021
Published Online27.04.2021

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