Research Article


DOI :10.26650/experimed.2021.898858   IUP :10.26650/experimed.2021.898858    Full Text (PDF)

Choice of Implant Surface-Feature May Affect the Viability of the Adherent Cells

Gül İpek GündoğanTayfun CıvakCenk Kığ

Objective: In vitro evaluation of implant materials' effects on cell adhesion and viability can provide useful information for predicting implant biocompatibility. Therefore by using a simple and inexpensive method, it was aimed to investigate whether different implant surface-features might have distinct effects on the viability and adherence of the cells. Material and Methods: Different dental implant surfaces (anodized (AN), blasted wrinkled (BW), grit/acid etched (GA), and hydroxylapatite sprayed (HB)) were tested for their possible effects on adhesion and viability of the adherent human osteoblast cells by using an agar-based in vitro technique. Viability of the cells was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and trypan blue staining. Results: The rate of cell adhesion did not seem to be significantly affected by the differences in surface features of dental implants (AN:78.21±0.52; BW:78.22±0.48; GA:78.44±0.85; HB:77.26±0.96). The surface features of the dental implants had an impact on the viability of the attached cells on the implants. Viability of the attached cells was significantly higher on AN, BW, GA surfaces when compared to the HB surface (AN: 72.28±6.04, BW: 67.02±3.47, GA: 85.82±5.05, and HB: 27.98±10.47). Conclusions: In vitro findings suggests that AN, BW, GA surfaces may provide a better platform than HB surfaces to maintain the viability of bound cells.

DOI :10.26650/experimed.2021.898858   IUP :10.26650/experimed.2021.898858    Full Text (PDF)

İmplant Yüzey Özelliği Seçimi Adherent Hücrelerin Canlılığını Etkileyebilir

Gül İpek GündoğanTayfun CıvakCenk Kığ

Amaç: İmplant materyallerinin hücre canlılığı ve hücre adezyonu üzerindeki in vitro etkilerinin değerlendirilmesi, implantların biyo-uyumluluklarının tahmin edilmesi yönünde değerli bilgiler sağlayabilir. Bu nedenle, basit ve maliyetli olmayan bir yöntem kullanılarak, farklı yüzey özelliklerine sahip implantların hücre canlılığı ve adezyonunu etkileyip etkilemeyeceğinin araştırılması amaçlanmıştır. Gereç ve Yöntemler: Farklı diş implant yüzeylerinin (anotlanmış (AN), patlatılmış buruşuk (BW), kum/asitle aşındırılmış (GA), püskürtülmüş hidroksi apatit yüzey (HB)) yapışma ve canlılık üzerindeki etkileri, adherent insan osteoblast hücre soyunda agar bazlı bir in vitro teknik kullanılarak karşılaştırılmıştır. Hücre canlılıklarının belirlenmesinde, 3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolium bromid (MTT) ve tripan mavisi boyama teknikleri uygulanmıştır. Bulgular: Hücre adezyonunun, dental implantların yüzey özelliklerindeki farklılıklardan önemli ölçüde etkilenmediği görülmüştür (AN: 78,21±0,52; BW: 78,22±0,48; GA: 78,44±0,85; HB: 77,26±0,96). Ancak, diş implantlarının yüzey özelliklerinin, implantlara tutunan hücrelerin canlılığı üzerinde etkisi olduğu tespit edilmiştir. Tutunan hücrelerin canlılığının, HB yüzeyine kıyasla AN, BW, GA yüzeylerinde anlamlı olarak daha yüksek olduğu bulunmuştur (AN: 72,28±6,04, BW: 67,02±3,47, GA: 85,82±5,05 ve HB: 27,98±10,47). Sonuçlar: Çalışma kapsamında yapılan in vitro deneylerden elde edilen bulgular; AN, BW, GA yüzeylerinin adherent hücrelerin canlılığını korumaları için HB yüzeylerinden daha iyi bir platform sağlayabileceğini göstermektedir.


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APA

Gündoğan, G.İ., Cıvak, T., & Kığ, C. (2021). Choice of Implant Surface-Feature May Affect the Viability of the Adherent Cells. Experimed, 11(1), 38-44. https://doi.org/10.26650/experimed.2021.898858


AMA

Gündoğan G İ, Cıvak T, Kığ C. Choice of Implant Surface-Feature May Affect the Viability of the Adherent Cells. Experimed. 2021;11(1):38-44. https://doi.org/10.26650/experimed.2021.898858


ABNT

Gündoğan, G.İ.; Cıvak, T.; Kığ, C. Choice of Implant Surface-Feature May Affect the Viability of the Adherent Cells. Experimed, [Publisher Location], v. 11, n. 1, p. 38-44, 2021.


Chicago: Author-Date Style

Gündoğan, Gül İpek, and Tayfun Cıvak and Cenk Kığ. 2021. “Choice of Implant Surface-Feature May Affect the Viability of the Adherent Cells.” Experimed 11, no. 1: 38-44. https://doi.org/10.26650/experimed.2021.898858


Chicago: Humanities Style

Gündoğan, Gül İpek, and Tayfun Cıvak and Cenk Kığ. Choice of Implant Surface-Feature May Affect the Viability of the Adherent Cells.” Experimed 11, no. 1 (Jul. 2021): 38-44. https://doi.org/10.26650/experimed.2021.898858


Harvard: Australian Style

Gündoğan, Gİ & Cıvak, T & Kığ, C 2021, 'Choice of Implant Surface-Feature May Affect the Viability of the Adherent Cells', Experimed, vol. 11, no. 1, pp. 38-44, viewed 25 Jul. 2021, https://doi.org/10.26650/experimed.2021.898858


Harvard: Author-Date Style

Gündoğan, G.İ. and Cıvak, T. and Kığ, C. (2021) ‘Choice of Implant Surface-Feature May Affect the Viability of the Adherent Cells’, Experimed, 11(1), pp. 38-44. https://doi.org/10.26650/experimed.2021.898858 (25 Jul. 2021).


MLA

Gündoğan, Gül İpek, and Tayfun Cıvak and Cenk Kığ. Choice of Implant Surface-Feature May Affect the Viability of the Adherent Cells.” Experimed, vol. 11, no. 1, 2021, pp. 38-44. [Database Container], https://doi.org/10.26650/experimed.2021.898858


Vancouver

Gündoğan Gİ, Cıvak T, Kığ C. Choice of Implant Surface-Feature May Affect the Viability of the Adherent Cells. Experimed [Internet]. 25 Jul. 2021 [cited 25 Jul. 2021];11(1):38-44. Available from: https://doi.org/10.26650/experimed.2021.898858 doi: 10.26650/experimed.2021.898858


ISNAD

Gündoğan, Gülİpek - Cıvak, Tayfun - Kığ, Cenk. Choice of Implant Surface-Feature May Affect the Viability of the Adherent Cells”. Experimed 11/1 (Jul. 2021): 38-44. https://doi.org/10.26650/experimed.2021.898858



TIMELINE


Submitted17.03.2021
Accepted02.04.2021
Published Online27.04.2021

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