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DOI :10.26650/experimed.1284215   IUP :10.26650/experimed.1284215    Full Text (PDF)

Comparison of Gene Expression Levels in the p53 Pathway in Blood and Bone Marrow of Healthy Individuals

Aynur Dağlar Aday, Gözde Öztan, İlknur Suer

Objective: A bone marrow (BM) sample is largely used in the diagnosis and prognosis follow-up of many hematological malignancies. BM aspiration is a more risky and laborious technique compared to blood collection. Together with publications in which the expression levels in BM and peripheral blood (PB) are correlated for many genes, there are also conflicting publications. This may also be due to the physiological and disease state. In this study, we aimed to compare the BM and PB expression levels of genes in the p53 pathway in healthy individuals.

Materials and Methods: The study comprised 23 healthy individuals. The expressions of 22 genes in the p53 pathway were analyzed using the RT2-profiler polymerase chain reaction (PCR) array. The expression levels were normalized to the reference gene β-actin. Then the mRNA expression levels between PB and BM sample groups were compared.

Results: The expression levels of the 20 genes studied were similar between the two groups. Only GADD45 and PTX3 genes were differentially expressed between PB and BM sample groups (p=0.003 and p=0.033, respectively) and those two gene expression levels were strongly correlated (r=0.886, p<0.0001).

Conclusion: When the expressions of 20 genes other than the GADD45 and PTX3 in our panel were evaluated, we suggest that PB largely reflects the p53 pathway gene expression levels in the BM. Therefore, PB may be preferred as an alternative to invasive BM in the analysis of these 20 genes in patients with hematological malignancies.

Keywords: p53 pathway, bone marrow, peripheral blood

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APA

Dağlar Aday, A., Öztan, G., & Suer, İ. (2023). Comparison of Gene Expression Levels in the p53 Pathway in Blood and Bone Marrow of Healthy Individuals. Experimed, 13(2), 103-108. https://doi.org/10.26650/experimed.1284215


AMA

Dağlar Aday A, Öztan G, Suer İ. Comparison of Gene Expression Levels in the p53 Pathway in Blood and Bone Marrow of Healthy Individuals. Experimed. 2023;13(2):103-108. https://doi.org/10.26650/experimed.1284215


ABNT

Dağlar Aday, A.; Öztan, G.; Suer, İ. Comparison of Gene Expression Levels in the p53 Pathway in Blood and Bone Marrow of Healthy Individuals. Experimed, [Publisher Location], v. 13, n. 2, p. 103-108, 2023.


Chicago: Author-Date Style

Dağlar Aday, Aynur, and Gözde Öztan and İlknur Suer. 2023. “Comparison of Gene Expression Levels in the p53 Pathway in Blood and Bone Marrow of Healthy Individuals.” Experimed 13, no. 2: 103-108. https://doi.org/10.26650/experimed.1284215


Chicago: Humanities Style

Dağlar Aday, Aynur, and Gözde Öztan and İlknur Suer. “Comparison of Gene Expression Levels in the p53 Pathway in Blood and Bone Marrow of Healthy Individuals.” Experimed 13, no. 2 (Sep. 2023): 103-108. https://doi.org/10.26650/experimed.1284215


Harvard: Australian Style

Dağlar Aday, A & Öztan, G & Suer, İ 2023, 'Comparison of Gene Expression Levels in the p53 Pathway in Blood and Bone Marrow of Healthy Individuals', Experimed, vol. 13, no. 2, pp. 103-108, viewed 30 Sep. 2023, https://doi.org/10.26650/experimed.1284215


Harvard: Author-Date Style

Dağlar Aday, A. and Öztan, G. and Suer, İ. (2023) ‘Comparison of Gene Expression Levels in the p53 Pathway in Blood and Bone Marrow of Healthy Individuals’, Experimed, 13(2), pp. 103-108. https://doi.org/10.26650/experimed.1284215 (30 Sep. 2023).


MLA

Dağlar Aday, Aynur, and Gözde Öztan and İlknur Suer. “Comparison of Gene Expression Levels in the p53 Pathway in Blood and Bone Marrow of Healthy Individuals.” Experimed, vol. 13, no. 2, 2023, pp. 103-108. [Database Container], https://doi.org/10.26650/experimed.1284215


Vancouver

Dağlar Aday A, Öztan G, Suer İ. Comparison of Gene Expression Levels in the p53 Pathway in Blood and Bone Marrow of Healthy Individuals. Experimed [Internet]. 30 Sep. 2023 [cited 30 Sep. 2023];13(2):103-108. Available from: https://doi.org/10.26650/experimed.1284215 doi: 10.26650/experimed.1284215


ISNAD

Dağlar Aday, Aynur - Öztan, Gözde - Suer, İlknur. “Comparison of Gene Expression Levels in the p53 Pathway in Blood and Bone Marrow of Healthy Individuals”. Experimed 13/2 (Sep. 2023): 103-108. https://doi.org/10.26650/experimed.1284215



TIMELINE


Submitted16.04.2023
Accepted16.06.2023
Published Online07.08.2023

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