Research Article


DOI :10.26650/experimed.2021.899430   IUP :10.26650/experimed.2021.899430    Full Text (PDF)

Effects of Exercise and Calorie Restriction on Brain and Testis in Natural Aging Model

Umay HakgüderÜnsal Veli ÜstündağBurçin Alev TüzünerNevin GençHazal İpekçiReyhan ÖzçelikTuğba Tunalı AkbayGöksel ŞenerAyşen YaratEbru Emekli Alturfan

The aim of our study was to examine the effects of exercise and calorie restriction on various tissue damage and antioxidant parameters in the brain and testis of rats in a natural aging model. For this purpose, male Sprague-Dawley rats were the control group (C), the elderly (A), the elderly with calorie restriction (ACR), the elderly who were exercised (AE) and the elderly who were exercised with calorie restriction (ACRE), they were divided into 5 groups. The control group was composed of three-month-old animals. The other group consisted of 15-month-old rats. Exercise and calorie restriction were applied for 6 weeks. At the end of the experiment, lipid peroxidation (LPO), nitric oxide (NO), glutathione (GSH) levels and superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST) and tissue factor (TF) were determined in brain and testicular tissues homogenates. As a result of the study, the A group’s brain and testis LPO, NO levels and TF activity increased, GSH levels and SOD, CAT and GST activities decreased, when compared to the C group. As a result of our study, an increase in oxidant damage was observed with TF activity in the brain and testis in the natural aging model, and positive effects of exercise and calorie restriction on the antioxidant levels in the brain were determined, especially in aging.

DOI :10.26650/experimed.2021.899430   IUP :10.26650/experimed.2021.899430    Full Text (PDF)

Doğal Yaşlanma Modelinde Egzersiz ve Kalori Kısıtlamasının Beyin ve Testiste Etkileri

Umay HakgüderÜnsal Veli ÜstündağBurçin Alev TüzünerNevin GençHazal İpekçiReyhan ÖzçelikTuğba Tunalı AkbayGöksel ŞenerAyşen YaratEbru Emekli Alturfan

Amacımız doğal yaşlanma modelinde egzersiz ve kalori kısıtlamasının sıçanlarda beyin ve testiste çeşitli doku hasarı ve antioksidan parametreler üzerine etkilerinin incelenmesidir. Bu amaçla Sprague-Dawley sıçanlar kontrol (K), yaşlı (Y), kalori kısıtlaması uygulanan yaşlı (YKK), egzersiz uygulanan yaşlı (YE) ve kalori kısıtlaması ile egzersiz uygulanan yaşlı (YKKE) olmak üzere 5 gruba ayrıldı. Kontrol grubu üç aylık genç hayvanlardan oluşturuldu. Diğer gruplar 15 aylık sıçanlardan oluşturuldu. Egzersiz ve kalori kısıtlaması 6 hafta boyunca uygulandı. Deney sonunda beyin ve testis dokuları homojenatlarında lipit peroksidasyon (LPO), nitrik oksit (NO), glutatyon (GSH) düzeyleri ile süperoksit dismutaz (SOD), katalaz (CAT), glutatyon-S-transferaz (GST) ve doku faktörü (DF) aktiviteleri tayin edildi. Çalışma sonucunda K grubu ile kıyaslandığında Y grubunda, beyin ve testis LPO, NO düzeyleri ve DF aktivitelerinin arttığı, GSH düzeyleri ile SOD, CAT ve GST aktivitelerinin ise azaldığı gözlendi. Doğal yaşlanma modelinde beyin ve testiste DF aktivitesi ile beraber oksidan hasarda artış gözlenmiş olup, özellikle yaşlanmada egzersiz ve kalori kısıtlamasının beyinde antioksidan düzeyleri üzerinde olumlu etkileri belirlenmiştir.


PDF View

References

  • 1. Ergün M. Yaşlılık ve Egzersiz. TJSM 2013; 48:131-8. google scholar
  • 2. Hawkins SA, Wiswell RA, Marcell TJ. Exercise and the master ath lete-a model of successful aging J Gerontol A Biol Sci Med Sci 2003; 58: 1009-11. [CrossRef] google scholar
  • 3. Wilmore J, Knuttgen H. Aerobic exercise and endurance improv ing fitness for health benefits. Phys Sportsmed 2003; 31(5): 45. [CrossRef] google scholar
  • 4. Blair SN, Kohl HW 3rd, Paffenbarger RS Jr, Clark DG, Cooper KH, Gibbons LW. Physical fitness and all-cause mortality. A prospective study of healthy men and women. JAMA 1989; 262: 2395-401. [CrossRef] google scholar
  • 5. Sohal RJ, Forster MJ. Caloric restriction and the aging process: a critique. Free Radic Biol Med 2014; 73: 366-82. [CrossRef] google scholar
  • 6. Segura B, Jurado MA. Metabolic syndrome and ageing: cognitive impairment and structural alterations of the central nervous system. Rev Neurol 2009; 49(8): 417-24. [CrossRef] google scholar
  • 7. Polat F, Kumral E. Normal ve patolojik beyin yaşlanması. Ege J Med 2010; 49(3): 3-10. google scholar
  • 8. Holmes RP, Goodman HO, Shihabi ZK, Jarow JP. The taurine and hypotaurine content of human semen. J Androl 1992; 13(3): 289- 92. google scholar
  • 9. Aitken RJ. Free radicals lipid peroxidation and sperm function. Reprod Fertil 1995; 7(4): 659-68. [CrossRef] google scholar
  • 10. Aitken RJ, DeIuliis GN. On the possible origins of DNA damage in human spermatozoa. Mol Hum Reprod 2010; 16(1): 3-13. [CrossRef] google scholar
  • 11. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measure ment with folin phenol reagent. J Biol Chem 1951; 193: 265-75. [CrossRef] google scholar
  • 12. Ledwozyw A, Michalak D, Stepien A, Kadziolka A. The relationship between plasma triglycerides, cholesterol, total lipids and lipid peroxidation products during human atherosclerosis. Clin Chim Acta 1986; 155: 275-83. [CrossRef] google scholar
  • 13. Ingram GIC, Hills M. Referance method for the one stage prothrombin time test on human blood. Thromb-Haemostas. 1976; 36: 237-8. [CrossRef] google scholar
  • 14. Miranda KM, Espey MG, Wink DA. A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide 2001; 5(1): 62-71. [CrossRef] google scholar
  • 15. Beutler E. Glutathione in red cell metabolism. In: A manuel of biochemical methods. 2nd ed, Grune and Stratton, NY,1975. p. 112-4. google scholar
  • 16. Mylorie AA, Collins H, Umbles C, Kyle J. Erythrocyte superoxide dismutase activity and other parameters of cupper status in rats ingesting lead acetate. Toxicol Appl Pharmacol 1986; 82: 512-20. [CrossRef] google scholar
  • 17. Aebi H. Catalase In vitro. In: Methods of Enzymatic Analysis. Ed: Bergmeyer HU, 2nd ed, FL,1974, p.121-6. google scholar
  • 18. Habig WH, Jacoby WB. Assays for differentation of glutathione-stransferases. Methods Enzymol 1981; 77: 398-405. [CrossRef] google scholar
  • 19. Gemma C, Vila J, Bachstetter A, Bickford PC. Oxidative stress and the aging brain: from theory to prevention. In: Riddle DR, editor. Brain Aging: Models, Methods, and Mechanisms. Boca Raton (FL): CRC Press/Taylor & Francis; 2007. Chapter 15. [CrossRef] google scholar
  • 20. Devi SA, Kiran TR. Regional responses in antioxidant system to exercise training and dietary vitamin E in aging rat brain. Neurobiol Aging 2004; 25(4): 501-8. [CrossRef] google scholar
  • 21. Sacher GA. Life table modification and life prolongation. In: Handbook of the biology of aging. Ed. Finch, CE., Hayflick, L. New York, Van Nostrand, 1977.p. 582-638. google scholar
  • 22. Demirsoy A. Yaşlanmanın ve ölümün evrimsel öyküsü. Geriatri 1998; 1(1):1-12. google scholar
  • 23. Tolmasoff JM, Ono T, Cutler RG. Superoxide dismutase: correlation with life span and specific metabolic rate in primate species. PNAS. 1980; 77: 2777-81. [CrossRef] google scholar
  • 24. Mendis-Handagama SM, Gelber SJ. Signs of aging are apparent in the testis interstitium of Sprague Dawley rats at 6 months of age. Tissue Cell 1995; 27(6): 689-99. [CrossRef] google scholar
  • 25. Peltola V, Huhtaniemi I, Ahotupa M. Antioxidant enzyme activity in the maturing rat testis. J Androl 1992; 13(5): 450-5. google scholar
  • 26. Mruk DD, Silvestrini B, Mo MY, Cheng CY. Antioxidant superoxide dismutase- A review: Its function, regulation in the testis, and role in male fertility. Contraception. 2002; 65: 305-11. [CrossRef] google scholar
  • 27. Kaur P, Bansal MP. Influence of selenium induced oxidative stress on spermatogenesis and lactate dehydrogenase-X in mice testis. Asian J Androl 2004; 6: 227-32. google scholar
  • 28. Somani SM, Husain K. Exercise training alters kinetics of antioxi dant enzymes in rat tissues. Biochem Mol Biol Int 1996; 38: 587-95. google scholar
  • 29. Manna I, Jana K, Samanta PK. Intensive swimming exercise-induced oxidative stress and reproductive dysfunction in male wistar rats: Protective role of alpha-tocopherol succinate. Can J Appl Physiol 2004; 29: 172-85. [CrossRef] google scholar
  • 30. Masoro EJ. Caloric restriction and aging: an update. Exp Gerontol 2000; 35: 299. [CrossRef] google scholar
  • 31. Cho Y, Cao X, Shen D, Tuo J, Parver L M., Rickles F R, et al. Evidence for enhanced tissue factor expression in age-related macular de generation. Lab Invest 2011; 91(4): 519-26. [CrossRef] google scholar
  • 32. Kazachkova N. Aging and Thrombosis: The Role of Genetic Factors. J Blood Disord 2016; 3(1): 1040 google scholar

Citations

Copy and paste a formatted citation or use one of the options to export in your chosen format


EXPORT



APA

Hakgüder, U., Üstündağ, Ü., Tüzüner, B.A., Genç, N., İpekçi, H., Özçelik, R., Tunalı Akbay, T., Şener, G., Yarat, A., & Emekli Alturfan, E. (2021). Effects of Exercise and Calorie Restriction on Brain and Testis in Natural Aging Model. Experimed, 11(1), 21-26. https://doi.org/10.26650/experimed.2021.899430


AMA

Hakgüder U, Üstündağ Ü, Tüzüner B A, Genç N, İpekçi H, Özçelik R, Tunalı Akbay T, Şener G, Yarat A, Emekli Alturfan E. Effects of Exercise and Calorie Restriction on Brain and Testis in Natural Aging Model. Experimed. 2021;11(1):21-26. https://doi.org/10.26650/experimed.2021.899430


ABNT

Hakgüder, U.; Üstündağ, Ü.; Tüzüner, B.A.; Genç, N.; İpekçi, H.; Özçelik, R.; Tunalı Akbay, T.; Şener, G.; Yarat, A.; Emekli Alturfan, E. Effects of Exercise and Calorie Restriction on Brain and Testis in Natural Aging Model. Experimed, [Publisher Location], v. 11, n. 1, p. 21-26, 2021.


Chicago: Author-Date Style

Hakgüder, Umay, and Ünsal Veli Üstündağ and Burçin Alev Tüzüner and Nevin Genç and Hazal İpekçi and Reyhan Özçelik and Tuğba Tunalı Akbay and Göksel Şener and Ayşen Yarat and Ebru Emekli Alturfan. 2021. “Effects of Exercise and Calorie Restriction on Brain and Testis in Natural Aging Model.” Experimed 11, no. 1: 21-26. https://doi.org/10.26650/experimed.2021.899430


Chicago: Humanities Style

Hakgüder, Umay, and Ünsal Veli Üstündağ and Burçin Alev Tüzüner and Nevin Genç and Hazal İpekçi and Reyhan Özçelik and Tuğba Tunalı Akbay and Göksel Şener and Ayşen Yarat and Ebru Emekli Alturfan. Effects of Exercise and Calorie Restriction on Brain and Testis in Natural Aging Model.” Experimed 11, no. 1 (Jul. 2021): 21-26. https://doi.org/10.26650/experimed.2021.899430


Harvard: Australian Style

Hakgüder, U & Üstündağ, Ü & Tüzüner, BA & Genç, N & İpekçi, H & Özçelik, R & Tunalı Akbay, T & Şener, G & Yarat, A & Emekli Alturfan, E 2021, 'Effects of Exercise and Calorie Restriction on Brain and Testis in Natural Aging Model', Experimed, vol. 11, no. 1, pp. 21-26, viewed 25 Jul. 2021, https://doi.org/10.26650/experimed.2021.899430


Harvard: Author-Date Style

Hakgüder, U. and Üstündağ, Ü. and Tüzüner, B.A. and Genç, N. and İpekçi, H. and Özçelik, R. and Tunalı Akbay, T. and Şener, G. and Yarat, A. and Emekli Alturfan, E. (2021) ‘Effects of Exercise and Calorie Restriction on Brain and Testis in Natural Aging Model’, Experimed, 11(1), pp. 21-26. https://doi.org/10.26650/experimed.2021.899430 (25 Jul. 2021).


MLA

Hakgüder, Umay, and Ünsal Veli Üstündağ and Burçin Alev Tüzüner and Nevin Genç and Hazal İpekçi and Reyhan Özçelik and Tuğba Tunalı Akbay and Göksel Şener and Ayşen Yarat and Ebru Emekli Alturfan. Effects of Exercise and Calorie Restriction on Brain and Testis in Natural Aging Model.” Experimed, vol. 11, no. 1, 2021, pp. 21-26. [Database Container], https://doi.org/10.26650/experimed.2021.899430


Vancouver

Hakgüder U, Üstündağ Ü, Tüzüner BA, Genç N, İpekçi H, Özçelik R, Tunalı Akbay T, Şener G, Yarat A, Emekli Alturfan E. Effects of Exercise and Calorie Restriction on Brain and Testis in Natural Aging Model. Experimed [Internet]. 25 Jul. 2021 [cited 25 Jul. 2021];11(1):21-26. Available from: https://doi.org/10.26650/experimed.2021.899430 doi: 10.26650/experimed.2021.899430


ISNAD

Hakgüder, Umay - Üstündağ, Ünsal Veli - Tüzüner, BurçinAlev - Genç, Nevin - İpekçi, Hazal - Özçelik, Reyhan - Tunalı Akbay, Tuğba - Şener, Göksel - Yarat, Ayşen - Emekli Alturfan, Ebru. Effects of Exercise and Calorie Restriction on Brain and Testis in Natural Aging Model”. Experimed 11/1 (Jul. 2021): 21-26. https://doi.org/10.26650/experimed.2021.899430



TIMELINE


Submitted18.03.2021
Accepted26.03.2021
Published Online27.04.2021

LICENCE


Attribution-NonCommercial (CC BY-NC)

This license lets others remix, tweak, and build upon your work non-commercially, and although their new works must also acknowledge you and be non-commercial, they don’t have to license their derivative works on the same terms.


SHARE




Istanbul University Press aims to contribute to the dissemination of ever growing scientific knowledge through publication of high quality scientific journals and books in accordance with the international publishing standards and ethics. Istanbul University Press follows an open access, non-commercial, scholarly publishing.