Research Article


DOI :10.26650/experimed.2021.928330   IUP :10.26650/experimed.2021.928330    Full Text (PDF)

Investigation of IBC-1, a Rare Plasmid-Mediated Class A Beta-lactamase in Members of Enterobacterales

Gülşen Altınkanat GelmezMünevver Ufuk Hasdemir GökboğaGüner Söyletir

Objective: The aim of the study was to investigate the presence of extended -spectrum beta-lactamases (ESBL) TEM, SHV, and CTX-M which are frequently observed all over the world, and the rare IBC-1 beta-lactamase enzyme. Material and Method: Thirty strains that isolated from various clinical samples from inpatient at Marmara University Hospital, which were phenotypically positive for ESBL, and IBC-1were included in the study. Antimicrobial susceptibility tests were performed both by disk diffusion and agar dilution tests. E-test and double-disc synergy method (DDS) were used for phenotypic detection of ESBLs. The presence of ESBL genes was detected by polymerase chain reaction (PCR). Results: All strains were susceptible to imipenem while ampicillin, amoxicillin-clavulanic acid, piperacillin, ceftazidime, and trimethoprim-sulfamethoxazole were resistant. While 4 strains were unidentified, 26 strains were detected as ESBL positive by E-test. Two strains were phenotypically positive for IBC-1 with DDS, while 5 strains were identified as doubtful. The rate of carrying the blaTEM, blaSHV, and blaCTX-M genes of strains was 73.3%, 60%, and 56.6%, respectively. The blaIBC gene was not detected in any of the strains. Conclusion: While the IBC-1 enzyme, which was first detected in Greece, have not caused a threat to our country yet, the rate of TEM, SHV, and CTX-M enzymes were found to be quite high.

DOI :10.26650/experimed.2021.928330   IUP :10.26650/experimed.2021.928330    Full Text (PDF)

Enterobacterales Üyelerinde Nadir Bir Plazmid Aracılı A Sınıfı Beta Laktamaz Olan IBC-1'in Araştırılması

Gülşen Altınkanat GelmezMünevver Ufuk Hasdemir GökboğaGüner Söyletir

Amaç: Bu çalışmanın amacı, tüm dünyada sıklıkla gözlemlenen geniş spektrumlu beta laktamazları (GSBL) TEM, SHV ve CTX-M ve nadir görülen IBC-1 beta laktamaz enziminin varlığını araştırmaktır. Gereç ve Yöntem: Marmara Üniversitesi Hastanesinde yatan hastaların klinik örneklerinden izole edilen, fenotipik olarak GSBL üreten ve IBC-1 fenotipi gösteren 30 köken çalışmaya dahil edilmiştir. Antibiyotik duyarlılık testleri disk difüzyon ve agarda dilüsyon yöntemi ile gerçekleştirilmiştir. GSBL enzimlerinin fenotipik olarak saptanmasında E-test ve çift disk sinerji yöntemi kullanılmıştır. GSBL üretiminden sorumlu olan genlerinin varlığını saptamak amacıyla polimeraz zincir reaksiyonu (PZR) yapılmıştır. Bulgular: Kökenlerin tamamı, imipeneme duyarlı bulunurken, ampisilin, amoksisilin klavulanik asit, piperasilin, seftazidim ve trimetoprim sülfametaksazole dirençli olarak; saptanmıştır. E-test yöntemiyle 4 köken tanımsız, 26 köken ise GSBL pozitif saptanmıştır. Çift disk sinerji yöntemi ile 2 köken fenotipik olarak IBC-1 pozitif iken, 5 köken şüpheli pozitif olarak belirlenmiştir. Kökenlerimizin blaTEM, blaSHV ve blaCTX-M genlerini taşıma oranı sırasıyla %73,3, %60 ve %56,6 olarak belirlenmiştir. blaIBC geni ise hiçbir kökende saptanmamıştır. Sonuç: İlk olarak Yunanistan’da saptanan IBC-1 enzimi ülkemiz için henüz bir tehlike oluşturmazken GSBL pozitif kökenlerde TEM, SHV ve CTX-M enzimlerinin oranı oldukça yüksek olarak tespit edilmiştir.


PDF View

References

  • 1. Paterson DL, Bonomo RA. Extended-spectrum beta-lactamases: a clinical update. Clin Microbiol Rev 2005; 18(4): 657-86. [CrossRef] google scholar
  • 2. Akova M. Dikkat Genişlemiş Spektrumlu Beta Laktamaz (GSBL) Var! ANKEM Dergisi 2004; 18(2): 98-103. google scholar
  • 3. Stürenburg E, Mack D. Extended Spectrum Beta Lactamases Imp-lications for The Clinical Microbiology Laboratory, Therapy and Infection Control. J Infect 2003; 47: 273-95. [CrossRef] google scholar
  • 4. Giakkoupi P, Tzouvelekis SL, Tsakris A, Loukova V, Sofianou D, Tze-lepi E. IBC-1 a Novel Integron Associated Class A Beta Lactamases with Extended Spectrum Properties Produced by an Enterobacter cloacae Clinical Strain. Antimicrob Agents Chemother 2000; 44(9): 2247-53. [CrossRef] google scholar
  • 5. Vourli S, Tzouvelekis SL, Tzelepi E, Lebessi NJ, Miriagou V. Chara-cterization of in In111 a Class 1 Integron That Carries the Exten-ded-Spectrum Beta-Lactamase Gene bla IBC-1. FEMS Microbiol Lett 2003; 225: 149-53. [CrossRef] google scholar
  • 6. Vourli S, Tzouvelekis SL, Tzelepi E. Characterization of Klebsiella pneumoniae Clinical Strain Producing a Rare Extended Spectrum Beta Lactamase (IBC-1). Int J Antimicrob Agents 2003; 21(5): 4957. [CrossRef] google scholar
  • 7. Tzelepi E, Managa C, Platsouka E, Sofianou D, Paniara O, Legakis NJ, et al. Extended Spectrum Beta Lactamase Types in Klebsiella pneumoniae and E. coli in Two Greek Hospitals. Int J Antimicrob Agents 2003; 21: 285-8. google scholar
  • 8. Lebessi E, Stamos G, Foustoukou M, Vourli S, Legakis NJ, Tzouvele-kis LS. Performance of Methods for Detection Of Extended- Spe-ctrum Beta-Lactamases Applied to Clinical Enterobacterial Strains Producing IBC-Type of Beta-Lactamases. J Clin Microbiol 2003; 41(2): 912. [CrossRef] google scholar
  • 9. Clinical and Laboratory Standards Institute. Performance Standar-ds for Antimicrobial Susceptibility Testing. 22th ed. CLSI Supple-ment M100. Wayne, PA: CLSI, 2004. google scholar
  • 10. Öksüz L, Gürler N. Escherichia coli ve Klebsiella spp. Suşlarında Genişlemiş Spektrumlu Beta Laktamazların Tiplendirilmesi ve Plaz-mid Profil Analizi. Mikrobiyoloji Bülteni 2009; 43: 183-94. google scholar
  • 11. Bradford PA. Extended Spectrum Beta Lactamases in the 21st Century: Characterization, Epidemiology, and Detection of This Important Resistance Threat. Clin Microbiol Rev 2001; 14(4): 93351. [CrossRef] google scholar
  • 12. Shaikh S, Fatima J, Shakil S, Rizvi SM, Kamal MA. Antibiotic resis-tance and extended spectrum beta-lactamases: Types, epidemio-logy and treatment. Saudi J Biol Sci 2015; 22(1): 90-101. [CrossRef] google scholar
  • 13. Naas T, Poirel L, Nordmann P. Minor extended-spectrum beta-lac-tamases. Clin Microbiol Infect 2008; Suppl 1: 42-52. google scholar
  • 14. Kartali G, Tzelepi E, Pournaras S, Kontopoulou C, Kontos F, Sofia-nou D, et al. Outbreak of Infections Caused by Enterobacter cloa-cae Producing the Integron-Associated Beta Lactamase IBC-1 in a Neonatal Intensive Care Unit of a Greek Hospital. Antimicrob Agents Chemother 2002; 46(5): 1577-80. [CrossRef] google scholar
  • 15. Bonnin RA, Jousset AB, Emeraud C, Oueslati S, Dortet L, Naas T. Genetic Diversity, Biochemical Properties, and Detection Methods of Minor Carbapenemases in Enterobacterales. Front Med (Lau-sanne) 2021; 20(7): 616490. [CrossRef] google scholar
  • 16. Cicek AC, Saral A, Iraz M, Ceylan A, Duzgun AO, Peleg AY, et al. OXA-and GES-type-lactamases predominate in extensively drug-resis-tant Acinetobacter baumannii isolates from a Turkish University Hospital. Clin Microbiol Infect 2014; 20: 410-5. [CrossRef] google scholar
  • 17. Naas T, Dortet L, Iorga BI. Structural and Functional Aspects of Class A Carbapenemases. Curr Drug Targets 2016; 17(9): 1006-28. [CrossRef] google scholar
  • 18. Bektaş A, Güdücüoğlu H, Gürsoy NC, Berktaş M, Gültepe BS, Parlak M, et al. Genişlemiş Spektrumlu Beta-Laktamaz (GSBL) Üreten Esc-herichia coli ve Klebsiella pneumoniae Suşlarının GSBL Genlerinin Araştırılması. FLORA Dergisi 2018; 23(3): 116-23. [CrossRef] google scholar
  • 19. Copur Cicek A, Saral A, Ozad Duzgun A, Yasar E, Cizmeci Z, Ozlem Balci P,et al. Nationwide study of Escherichia coli producing extended-spectrum β-lactamases TEM, SHV and CTX-M in Turkey. J Antibiot (Tokyo) 2013; 66(11): 647-50. [CrossRef] google scholar
  • 20. Dağlar D, Ongüt G, Oğünç D, Ozhak Baysan B, Demirbakan H, et al. Toplum kaynaklı üriner sistem enfeksiyonu etkeni Escherichia coli suşlarında GSBL enzim tiplerinin izoelektrik odaklama ve poli-meraz zincir reaksiyonu yöntemleri ile araştırılması. Mikrobiyol Bul 2010; 44(3): 367-74. google scholar

Citations

Copy and paste a formatted citation or use one of the options to export in your chosen format


EXPORT



APA

Altınkanat Gelmez, G., Hasdemir Gökboğa, M.U., & Söyletir, G. (2021). Investigation of IBC-1, a Rare Plasmid-Mediated Class A Beta-lactamase in Members of Enterobacterales. Experimed, 11(2), 81-87. https://doi.org/10.26650/experimed.2021.928330


AMA

Altınkanat Gelmez G, Hasdemir Gökboğa M U, Söyletir G. Investigation of IBC-1, a Rare Plasmid-Mediated Class A Beta-lactamase in Members of Enterobacterales. Experimed. 2021;11(2):81-87. https://doi.org/10.26650/experimed.2021.928330


ABNT

Altınkanat Gelmez, G.; Hasdemir Gökboğa, M.U.; Söyletir, G. Investigation of IBC-1, a Rare Plasmid-Mediated Class A Beta-lactamase in Members of Enterobacterales. Experimed, [Publisher Location], v. 11, n. 2, p. 81-87, 2021.


Chicago: Author-Date Style

Altınkanat Gelmez, Gülşen, and Münevver Ufuk Hasdemir Gökboğa and Güner Söyletir. 2021. “Investigation of IBC-1, a Rare Plasmid-Mediated Class A Beta-lactamase in Members of Enterobacterales.” Experimed 11, no. 2: 81-87. https://doi.org/10.26650/experimed.2021.928330


Chicago: Humanities Style

Altınkanat Gelmez, Gülşen, and Münevver Ufuk Hasdemir Gökboğa and Güner Söyletir. Investigation of IBC-1, a Rare Plasmid-Mediated Class A Beta-lactamase in Members of Enterobacterales.” Experimed 11, no. 2 (Sep. 2021): 81-87. https://doi.org/10.26650/experimed.2021.928330


Harvard: Australian Style

Altınkanat Gelmez, G & Hasdemir Gökboğa, MU & Söyletir, G 2021, 'Investigation of IBC-1, a Rare Plasmid-Mediated Class A Beta-lactamase in Members of Enterobacterales', Experimed, vol. 11, no. 2, pp. 81-87, viewed 24 Sep. 2021, https://doi.org/10.26650/experimed.2021.928330


Harvard: Author-Date Style

Altınkanat Gelmez, G. and Hasdemir Gökboğa, M.U. and Söyletir, G. (2021) ‘Investigation of IBC-1, a Rare Plasmid-Mediated Class A Beta-lactamase in Members of Enterobacterales’, Experimed, 11(2), pp. 81-87. https://doi.org/10.26650/experimed.2021.928330 (24 Sep. 2021).


MLA

Altınkanat Gelmez, Gülşen, and Münevver Ufuk Hasdemir Gökboğa and Güner Söyletir. Investigation of IBC-1, a Rare Plasmid-Mediated Class A Beta-lactamase in Members of Enterobacterales.” Experimed, vol. 11, no. 2, 2021, pp. 81-87. [Database Container], https://doi.org/10.26650/experimed.2021.928330


Vancouver

Altınkanat Gelmez G, Hasdemir Gökboğa MU, Söyletir G. Investigation of IBC-1, a Rare Plasmid-Mediated Class A Beta-lactamase in Members of Enterobacterales. Experimed [Internet]. 24 Sep. 2021 [cited 24 Sep. 2021];11(2):81-87. Available from: https://doi.org/10.26650/experimed.2021.928330 doi: 10.26650/experimed.2021.928330


ISNAD

Altınkanat Gelmez, Gülşen - Hasdemir Gökboğa, MünevverUfuk - Söyletir, Güner. Investigation of IBC-1, a Rare Plasmid-Mediated Class A Beta-lactamase in Members of Enterobacterales”. Experimed 11/2 (Sep. 2021): 81-87. https://doi.org/10.26650/experimed.2021.928330



TIMELINE


Submitted27.04.2021
Accepted04.07.2021
Published Online25.08.2021

LICENCE


Attribution-NonCommercial (CC BY-NC)

This license lets others remix, tweak, and build upon your work non-commercially, and although their new works must also acknowledge you and be non-commercial, they don’t have to license their derivative works on the same terms.


SHARE




Istanbul University Press aims to contribute to the dissemination of ever growing scientific knowledge through publication of high quality scientific journals and books in accordance with the international publishing standards and ethics. Istanbul University Press follows an open access, non-commercial, scholarly publishing.