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DOI :10.26650/experimed.1260973   IUP :10.26650/experimed.1260973    Full Text (PDF)

Evaluation of DNA Microarray in Biomarker Detection in Cell-free DNA from Colorectal Cancer Cell Lines: A Proof-of-Concept Study

Hasan Hüseyin Kazan, Ceyhan Pırıl Karahan, Ekin Çelik, Ahmet Çağlar Özketen, Duygu Birgücü Çağıl, Mehmet Ali Ergün

Objective: DNA microarray is a powerful method to identify genomic anomalies including small insertions, duplications and/or deletions. This method is widely used in routine genetic screening for explaining the genetic background of certain phenotypes, for example, cancer. Cell-free DNA (cfDNA), which is an approach that may give information about the somatic tissues in peripheral blood, is another popular method used in routine genetic screening to understand the background of particular phenotypes, one of which is cancer. There is limited available research that investigates the involvement of these two approaches to decipher novel cancer biomarkers in the literature. However, detection of cancer biomarkers, especially non-invasive types, has been of great interest to research groups.

Materials and Methods: In the present study, we used colorectal cancer as a model tumor to figure out whether we could determine definite biomarkers from cfDNA using DNA microarray methodology. We isolated cfDNA from the cell-free mediums of the cultures of colorectal cancer cell lines in the presence of the control group which was the healthy epithelial colon cell line.

Results: Our results underlined significant alterations that were deletions and/or duplications in some of the genomic regions in a cell line-specific manner.

Conclusion: We propose that DNA microarray be used to assess the sub-types of certain cancers in a non-invasive manner using cfDNA approaches

Keywords: cfDNA, carcinogenesis, colorectal cancer, DNA microarray, biomarker

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APA

Kazan, H.H., Pırıl Karahan, C., Çelik, E., Özketen, A.Ç., Birgücü Çağıl, D., & Ergün, M.A. (2023). Evaluation of DNA Microarray in Biomarker Detection in Cell-free DNA from Colorectal Cancer Cell Lines: A Proof-of-Concept Study. Experimed, 13(2), 86-92. https://doi.org/10.26650/experimed.1260973


AMA

Kazan H H, Pırıl Karahan C, Çelik E, Özketen A Ç, Birgücü Çağıl D, Ergün M A. Evaluation of DNA Microarray in Biomarker Detection in Cell-free DNA from Colorectal Cancer Cell Lines: A Proof-of-Concept Study. Experimed. 2023;13(2):86-92. https://doi.org/10.26650/experimed.1260973


ABNT

Kazan, H.H.; Pırıl Karahan, C.; Çelik, E.; Özketen, A.Ç.; Birgücü Çağıl, D.; Ergün, M.A. Evaluation of DNA Microarray in Biomarker Detection in Cell-free DNA from Colorectal Cancer Cell Lines: A Proof-of-Concept Study. Experimed, [Publisher Location], v. 13, n. 2, p. 86-92, 2023.


Chicago: Author-Date Style

Kazan, Hasan Hüseyin, and Ceyhan Pırıl Karahan and Ekin Çelik and Ahmet Çağlar Özketen and Duygu Birgücü Çağıl and Mehmet Ali Ergün. 2023. “Evaluation of DNA Microarray in Biomarker Detection in Cell-free DNA from Colorectal Cancer Cell Lines: A Proof-of-Concept Study.” Experimed 13, no. 2: 86-92. https://doi.org/10.26650/experimed.1260973


Chicago: Humanities Style

Kazan, Hasan Hüseyin, and Ceyhan Pırıl Karahan and Ekin Çelik and Ahmet Çağlar Özketen and Duygu Birgücü Çağıl and Mehmet Ali Ergün. “Evaluation of DNA Microarray in Biomarker Detection in Cell-free DNA from Colorectal Cancer Cell Lines: A Proof-of-Concept Study.” Experimed 13, no. 2 (Sep. 2023): 86-92. https://doi.org/10.26650/experimed.1260973


Harvard: Australian Style

Kazan, HH & Pırıl Karahan, C & Çelik, E & Özketen, AÇ & Birgücü Çağıl, D & Ergün, MA 2023, 'Evaluation of DNA Microarray in Biomarker Detection in Cell-free DNA from Colorectal Cancer Cell Lines: A Proof-of-Concept Study', Experimed, vol. 13, no. 2, pp. 86-92, viewed 30 Sep. 2023, https://doi.org/10.26650/experimed.1260973


Harvard: Author-Date Style

Kazan, H.H. and Pırıl Karahan, C. and Çelik, E. and Özketen, A.Ç. and Birgücü Çağıl, D. and Ergün, M.A. (2023) ‘Evaluation of DNA Microarray in Biomarker Detection in Cell-free DNA from Colorectal Cancer Cell Lines: A Proof-of-Concept Study’, Experimed, 13(2), pp. 86-92. https://doi.org/10.26650/experimed.1260973 (30 Sep. 2023).


MLA

Kazan, Hasan Hüseyin, and Ceyhan Pırıl Karahan and Ekin Çelik and Ahmet Çağlar Özketen and Duygu Birgücü Çağıl and Mehmet Ali Ergün. “Evaluation of DNA Microarray in Biomarker Detection in Cell-free DNA from Colorectal Cancer Cell Lines: A Proof-of-Concept Study.” Experimed, vol. 13, no. 2, 2023, pp. 86-92. [Database Container], https://doi.org/10.26650/experimed.1260973


Vancouver

Kazan HH, Pırıl Karahan C, Çelik E, Özketen AÇ, Birgücü Çağıl D, Ergün MA. Evaluation of DNA Microarray in Biomarker Detection in Cell-free DNA from Colorectal Cancer Cell Lines: A Proof-of-Concept Study. Experimed [Internet]. 30 Sep. 2023 [cited 30 Sep. 2023];13(2):86-92. Available from: https://doi.org/10.26650/experimed.1260973 doi: 10.26650/experimed.1260973


ISNAD

Kazan, HasanHüseyin - Pırıl Karahan, Ceyhan - Çelik, Ekin - Özketen, AhmetÇağlar - Birgücü Çağıl, Duygu - Ergün, MehmetAli. “Evaluation of DNA Microarray in Biomarker Detection in Cell-free DNA from Colorectal Cancer Cell Lines: A Proof-of-Concept Study”. Experimed 13/2 (Sep. 2023): 86-92. https://doi.org/10.26650/experimed.1260973



TIMELINE


Submitted07.03.2023
Accepted05.06.2023
Published Online04.08.2023

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