Large-Scale Proteomic Analysis of Patients with Type 2 Diabetes Mellitus and Atherosclerosis Using a Label-Free LC-MS/MS Approach
Mustafa Gani Sürmen, Tijen Alkan Bozkaya, Mehmet Şanser Ateş, Saime Sürmen, Çağrı Çakıcı, Sadrettin Pençe, Nesrin EmekliObjective: Type 2 diabetes mellitus (T2D) is a metabolic disease whose molecular events have not yet been fully clarified. However, nextgeneration powerful molecular approaches such as mass spectrometry (MS)-based proteomics holds promise. In this study, we aimed to reveal the protein profile of serum samples obtained from patients with T2D and atherosclerotic cardiovascular disease using the highresolution liquid chromatography (LC)-MS/MS system. Materials and Methods: Immune depletion was performed for the top 12 abundant proteins in 10 µl serum samples taken from individuals. Then, tryptic peptides were obtained from total proteins by applying a digestion protocol. Accordingly, reduction, alkylation, and digestion with trypsin enzyme were carried out, respectively. Tryptic peptides were analyzed in an ultra-high-pressure LC-MS/MS system with a label-free proteomic approach. The raw data were processed using the software program. Results: LC-MS/MS analyses revealed 120 proteins with significant expression changes. Some of these proteins were associated with inflammation, lipid transport, and oxidative stress, which are known to play an important role in T2D and its complications. Conclusion: As a result, LC-MS/MS analyses highlighted the proteins that will provide predictions in the treatment and course of T2D. We believe that validation of these proteins with targeted proteomic approaches in a larger sample in further studies will contribute to the development of clinically usable panels.
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References
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APA
Sürmen, M., Alkan Bozkaya, T., Ateş, M.Ş., Sürmen, S., Çakıcı, Ç., Pençe, S., & Emekli, N. (2023). Large-Scale Proteomic Analysis of Patients with Type 2 Diabetes Mellitus and Atherosclerosis Using a Label-Free LC-MS/MS Approach. Experimed, 13(1), 26-38. https://doi.org/10.26650/experimed.1219362
AMA
Sürmen M, Alkan Bozkaya T, Ateş M Ş, Sürmen S, Çakıcı Ç, Pençe S, Emekli N. Large-Scale Proteomic Analysis of Patients with Type 2 Diabetes Mellitus and Atherosclerosis Using a Label-Free LC-MS/MS Approach. Experimed. 2023;13(1):26-38. https://doi.org/10.26650/experimed.1219362
ABNT
Sürmen, M.; Alkan Bozkaya, T.; Ateş, M.Ş.; Sürmen, S.; Çakıcı, Ç.; Pençe, S.; Emekli, N. Large-Scale Proteomic Analysis of Patients with Type 2 Diabetes Mellitus and Atherosclerosis Using a Label-Free LC-MS/MS Approach. Experimed, [Publisher Location], v. 13, n. 1, p. 26-38, 2023.
Chicago: Author-Date Style
Sürmen, Mustafa Gani, and Tijen Alkan Bozkaya and Mehmet Şanser Ateş and Saime Sürmen and Çağrı Çakıcı and Sadrettin Pençe and Nesrin Emekli. 2023. “Large-Scale Proteomic Analysis of Patients with Type 2 Diabetes Mellitus and Atherosclerosis Using a Label-Free LC-MS/MS Approach.” Experimed 13, no. 1: 26-38. https://doi.org/10.26650/experimed.1219362
Chicago: Humanities Style
Sürmen, Mustafa Gani, and Tijen Alkan Bozkaya and Mehmet Şanser Ateş and Saime Sürmen and Çağrı Çakıcı and Sadrettin Pençe and Nesrin Emekli. “Large-Scale Proteomic Analysis of Patients with Type 2 Diabetes Mellitus and Atherosclerosis Using a Label-Free LC-MS/MS Approach.” Experimed 13, no. 1 (Dec. 2024): 26-38. https://doi.org/10.26650/experimed.1219362
Harvard: Australian Style
Sürmen, M & Alkan Bozkaya, T & Ateş, MŞ & Sürmen, S & Çakıcı, Ç & Pençe, S & Emekli, N 2023, 'Large-Scale Proteomic Analysis of Patients with Type 2 Diabetes Mellitus and Atherosclerosis Using a Label-Free LC-MS/MS Approach', Experimed, vol. 13, no. 1, pp. 26-38, viewed 4 Dec. 2024, https://doi.org/10.26650/experimed.1219362
Harvard: Author-Date Style
Sürmen, M. and Alkan Bozkaya, T. and Ateş, M.Ş. and Sürmen, S. and Çakıcı, Ç. and Pençe, S. and Emekli, N. (2023) ‘Large-Scale Proteomic Analysis of Patients with Type 2 Diabetes Mellitus and Atherosclerosis Using a Label-Free LC-MS/MS Approach’, Experimed, 13(1), pp. 26-38. https://doi.org/10.26650/experimed.1219362 (4 Dec. 2024).
MLA
Sürmen, Mustafa Gani, and Tijen Alkan Bozkaya and Mehmet Şanser Ateş and Saime Sürmen and Çağrı Çakıcı and Sadrettin Pençe and Nesrin Emekli. “Large-Scale Proteomic Analysis of Patients with Type 2 Diabetes Mellitus and Atherosclerosis Using a Label-Free LC-MS/MS Approach.” Experimed, vol. 13, no. 1, 2023, pp. 26-38. [Database Container], https://doi.org/10.26650/experimed.1219362
Vancouver
Sürmen M, Alkan Bozkaya T, Ateş MŞ, Sürmen S, Çakıcı Ç, Pençe S, Emekli N. Large-Scale Proteomic Analysis of Patients with Type 2 Diabetes Mellitus and Atherosclerosis Using a Label-Free LC-MS/MS Approach. Experimed [Internet]. 4 Dec. 2024 [cited 4 Dec. 2024];13(1):26-38. Available from: https://doi.org/10.26650/experimed.1219362 doi: 10.26650/experimed.1219362
ISNAD
Sürmen, Mustafa Gani - Alkan Bozkaya, Tijen - Ateş, MehmetŞanser - Sürmen, Saime - Çakıcı, Çağrı - Pençe, Sadrettin - Emekli, Nesrin. “Large-Scale Proteomic Analysis of Patients with Type 2 Diabetes Mellitus and Atherosclerosis Using a Label-Free LC-MS/MS Approach”. Experimed 13/1 (Dec. 2024): 26-38. https://doi.org/10.26650/experimed.1219362