Research Article


DOI :10.26650/experimed.2021.953435   IUP :10.26650/experimed.2021.953435    Full Text (PDF)

The Biocompatibility of Sodium Lauryl Sulphate on Developing Zebrafish Embryos

Simge MeşeliGül KaplanDerya CansızÜnsal Veli Üstündağİsmail ÜnalEbru Emekli AlturfanFunda YanıkoğluDilek Tağtekin

Objective: As an anionic surfactant, sodium lauryl sulphate (SLS), is used as an emulsifying agent in toothpastes as well as many different household cleaning products. Many toothpastes contain SLS and it is responsible for the formation of foam when brushing. However SLS may also irritate sensitive teeth and gums. Lipid peroxidation (LPO) is defined as an autoxidation process that is induced through the attack of free oxygen radicals leading to oxidative stress in the pathophysiology of various diseases. LPO causes the formation of highly reactive products including different aldehydes, ketones, and alkanes serving as biomarkers of LPO. In our study we aimed to expose zebrafish embryos to SLS and determine LPO in SLS exposed zebrafish embryos. Material and Method: Zebrafish embryos that were dividing normally and which had spherical shapes were chosen and they were exposed to SLS both in low and high concentrations in well plates for 72 hours. Rates of mortality and hatching were determined. The levels of malondialdehyde were evaluated using the Yagi's method as the end products of LPO in the form of thiobarbituric acid reactive substances. Results: Our findings showed LPO increased significantly in both low SLS (p<0.05) and high dose SLS (p<0.05) exposed zebrafish embryos when they were compared to the control group. Conclusion: It may be suggested that LPO is an early indicator of exposure to SLS during embryogenesis and further studies are required to confirm this finding.

DOI :10.26650/experimed.2021.953435   IUP :10.26650/experimed.2021.953435    Full Text (PDF)

Sodyum Lauril Sülfatın Gelişmekte Olan Zebra Balığı Embriyolarında Biyouyumluluğu

Simge MeşeliGül KaplanDerya CansızÜnsal Veli Üstündağİsmail ÜnalEbru Emekli AlturfanFunda YanıkoğluDilek Tağtekin

Amaç: Sodyum lauril sülfat (SLS), ev temizlik ürünleri ve diş macunlarında emülsifiye edici temizlik maddesi olarak kullanılan anyonik bir yüzey aktif maddedir. Birçok diş macunu fırçalarken köpük oluşumuna yol açan SLS içerir. Ancak SLS hassas diş ve diş etlerini tahriş edebilir. Lipid peroksidasyonu (LPO), çeşitli hastalıkların patofizyolojisinde oksidatif strese yol açan serbest radikallerin saldırısı ile başlatılan bir otooksidasyon sürecidir. LPO, LPO'nun biyobelirteçleri olarak görev yapan farklı aldehitler, ketonlar, alkanlar dahil olmak üzere reaktif ürünlerin oluşumuna neden olur. Çalışmamızın amacı, SLS'ye maruz kalan zebra balığı embriyolarında oluşan LPO'yu değerlendirmektir. Gereç ve Yöntem: Normal olarak bölünen küresel embriyolar, 72 saat boyunca plaka kuyucuklarında düşük ve yüksek dozda SLS'ye maruz bırakılmıştır. Mortalite ve kuluçkadan çıkma oranları belirlenmiştir. Tiyobarbitürik asit reaktif maddeler olarak LPO'nun son ürünü olan malondialdehit (MDA) düzeyini belirlemek için Yagi yöntemi kullanılmıştır. Bulgular: Bulgularımız, hem düşük SLS (p<0,05) hem de yüksek doz SLS’ye (p<0,05) maruz kalan zebra balığı embriyolarında kontrol grubu ile karşılaştırıldığında LPO'nun önemli ölçüde arttığını göstermiştir. Sonuç: LPO'nun embriyogenez sırasında SLS'ye maruz kalmanın erken bir göstergesi olduğu öne sürülebilir ve bu bulguyu doğrulamak için farklı çalışmaların yapılması gerektiği düşünülmektedir


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APA

Meşeli, S., Kaplan, G., Cansız, D., Üstündağ, Ü.V., Ünal, İ., Emekli Alturfan, E., Yanıkoğlu, F., & Tağtekin, D. (2021). The Biocompatibility of Sodium Lauryl Sulphate on Developing Zebrafish Embryos. Experimed, 11(2), 67-72. https://doi.org/10.26650/experimed.2021.953435


AMA

Meşeli S, Kaplan G, Cansız D, Üstündağ Ü V, Ünal İ, Emekli Alturfan E, Yanıkoğlu F, Tağtekin D. The Biocompatibility of Sodium Lauryl Sulphate on Developing Zebrafish Embryos. Experimed. 2021;11(2):67-72. https://doi.org/10.26650/experimed.2021.953435


ABNT

Meşeli, S.; Kaplan, G.; Cansız, D.; Üstündağ, Ü.V.; Ünal, İ.; Emekli Alturfan, E.; Yanıkoğlu, F.; Tağtekin, D. The Biocompatibility of Sodium Lauryl Sulphate on Developing Zebrafish Embryos. Experimed, [Publisher Location], v. 11, n. 2, p. 67-72, 2021.


Chicago: Author-Date Style

Meşeli, Simge, and Gül Kaplan and Derya Cansız and Ünsal Veli Üstündağ and İsmail Ünal and Ebru Emekli Alturfan and Funda Yanıkoğlu and Dilek Tağtekin. 2021. “The Biocompatibility of Sodium Lauryl Sulphate on Developing Zebrafish Embryos.” Experimed 11, no. 2: 67-72. https://doi.org/10.26650/experimed.2021.953435


Chicago: Humanities Style

Meşeli, Simge, and Gül Kaplan and Derya Cansız and Ünsal Veli Üstündağ and İsmail Ünal and Ebru Emekli Alturfan and Funda Yanıkoğlu and Dilek Tağtekin. The Biocompatibility of Sodium Lauryl Sulphate on Developing Zebrafish Embryos.” Experimed 11, no. 2 (Sep. 2021): 67-72. https://doi.org/10.26650/experimed.2021.953435


Harvard: Australian Style

Meşeli, S & Kaplan, G & Cansız, D & Üstündağ, ÜV & Ünal, İ & Emekli Alturfan, E & Yanıkoğlu, F & Tağtekin, D 2021, 'The Biocompatibility of Sodium Lauryl Sulphate on Developing Zebrafish Embryos', Experimed, vol. 11, no. 2, pp. 67-72, viewed 24 Sep. 2021, https://doi.org/10.26650/experimed.2021.953435


Harvard: Author-Date Style

Meşeli, S. and Kaplan, G. and Cansız, D. and Üstündağ, Ü.V. and Ünal, İ. and Emekli Alturfan, E. and Yanıkoğlu, F. and Tağtekin, D. (2021) ‘The Biocompatibility of Sodium Lauryl Sulphate on Developing Zebrafish Embryos’, Experimed, 11(2), pp. 67-72. https://doi.org/10.26650/experimed.2021.953435 (24 Sep. 2021).


MLA

Meşeli, Simge, and Gül Kaplan and Derya Cansız and Ünsal Veli Üstündağ and İsmail Ünal and Ebru Emekli Alturfan and Funda Yanıkoğlu and Dilek Tağtekin. The Biocompatibility of Sodium Lauryl Sulphate on Developing Zebrafish Embryos.” Experimed, vol. 11, no. 2, 2021, pp. 67-72. [Database Container], https://doi.org/10.26650/experimed.2021.953435


Vancouver

Meşeli S, Kaplan G, Cansız D, Üstündağ ÜV, Ünal İ, Emekli Alturfan E, Yanıkoğlu F, Tağtekin D. The Biocompatibility of Sodium Lauryl Sulphate on Developing Zebrafish Embryos. Experimed [Internet]. 24 Sep. 2021 [cited 24 Sep. 2021];11(2):67-72. Available from: https://doi.org/10.26650/experimed.2021.953435 doi: 10.26650/experimed.2021.953435


ISNAD

Meşeli, Simge - Kaplan, Gül - Cansız, Derya - Üstündağ, ÜnsalVeli - Ünal, İsmail - Emekli Alturfan, Ebru - Yanıkoğlu, Funda - Tağtekin, Dilek. The Biocompatibility of Sodium Lauryl Sulphate on Developing Zebrafish Embryos”. Experimed 11/2 (Sep. 2021): 67-72. https://doi.org/10.26650/experimed.2021.953435



TIMELINE


Submitted17.06.2021
Accepted30.07.2021
Published Online25.08.2021

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