Research Article


DOI :10.26650/IstanbulJPharm.2021.881724   IUP :10.26650/IstanbulJPharm.2021.881724    Full Text (PDF)

Effects of chlorpyrifos-methyl, chlormequat, deltamethrin, glyphosate, pirimiphos-methyl, tebuconazole and their mixture on oxidative stress and toxicity in HUVEC cell line

Çiğdem SevimAli TaghizadehghalehjoughiMehtap Kara

Background and Aims: Humans and animals have daily contact with various chemicals, including food additives, pesticides, antibiotics, other veterinary drugs, and other xenobiotics. Pesticide exposure causes many health disorders. Mixed exposure to pesticides is an important issue for human and environmental health. Methods: In this study, we have determined the cytotoxicity of chlormequat pirimiphos-methyl, glyphosate, tebuconazole, chlorpyrifos-methyl, deltamethrin, and the mixture of these six pesticides. We further investigated the role of oxidative stress, total oxidant status (TOS), lactate dehydrogenase (LDH) and antioxidant defense mechanism TOS, total glutathione (GSH) levels with the observed cytotoxicity. Results: In this study, the mixtures of pesticides reduced total antioxidant status (TAS) and GSH level one by one and increased the reactive oxygen species (ROS) generation in HUVECs, respectively. The results also showed a significant contribution of oxidative stress on cytotoxicity during pesticide mixture exposure. Conclusion: The findings are that pesticide mixture exposure might have an impact on human health risk at contaminated sites and under occupational exposure conditions.


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References

  • Abdel-Daim, M., El-Bialy, B. E., Rahman, H. G., Radi, A. M., Hefny, H. A., & Hassan, A. M. (2016). Antagonistic effects of Spirulina platen-sis against sub-acute deltamethrin toxicity in mice: Biochemical and histopathological studies. Biomedicine & Pharmacotherapy= Biomedecine & Pharmacotherapie, 77, 79-85. google scholar
  • Abdollahi, M., Ranjbar, A., Shadnia, S., Nikfar, S., & Rezaie, A. (2004). Pesticides and oxidative stress: a review. Medical Science Moni-tor: International Medical Journal of Experimental and Clinical Re-search, 10(6), RA141-RA147. google scholar
  • Agrawal, A., & Sharma, B., (2010). Pesticides induced oxidative stress in mammalian systems. International Journal of Biological and Medical Research, 1(3), 90-104. google scholar
  • Anogwih, J. A. (2014). Toxicity of pirimiphos methyl (Actellic 25EC) on Anopheles gambiae s.s., Culex quinquefasciatus (Diptera: Cu-licidae), and potential biocontrol agent, Poecilia reticulata (Pisces: Poeciliidae). Journal of Economic Entomology, 107(4), 1440-1446. google scholar
  • Bagherpour Shamloo, H., Golkari, S., Faghfoori, Z., Movassagh-pour, A., Lotfi, H., Barzegari, A., & Yari Khosroushahi, A. (2016). Lactobacillus Casei Decreases Organophosphorus Pesticide Di-azinon Cytotoxicity in Human HUVEC Cell Line. Advanced Phar-maceutical Bulletin, 6(2), 201-210. google scholar
  • Banaee, M., Akhlaghi, M., Soltanian, S., Gholamhosseini, A., Heidar-ieh, H., & Fereidouni, M. S. (2019). “Acute exposure to chlorpyrifos and glyphosate induces changes in hemolymph biochemical parameters in the crayfish, Astacus leptodactylus (Eschscholtz, 1823)”. Comparative Biochemistry and Physiology. Toxicology & Pharmacology, 222, 145-155. google scholar
  • Ben Othmene, Y., Hamdi, H., Annabi, E., Amara, I., Ben Salem, I., Neffati, F., Najjar, M. F., Abid-Essefi, S. (2020). Tebuconazole in-duced cardiotoxicity in male adult rat. Food and chemical toxi-cology An International Journal Published for the British Industrial Biological Research Association, 137, 111134. google scholar
  • Benachour, N., & Seralini, G. E. (2009). Glyphosate formulations induce apoptosis and necrosis in human umbilical, embryonic, and placental cells. Chemical Research in Toxicology, 22(1), 97-105. google scholar
  • Berrouague, S., Rouag, M., Khaldi, T., Boumendjel, A., Boumendjel, M., Taibi, F., & Messarah, M. (2019). Efficacy of Allium sativum oil to allevi-ate tebuconazol-induced oxidative stress in the liver of adult rats. Cellular and Molecular Biology (Noisy-le-Grand, France), 65(8), 23-31. google scholar
  • Bhattacharjee, P., Borah, A., & Das, S. (2020). Quercetin-induced amelioration of Deltamethrin stress in freshwater teleost, Channa punctata: Multiple biomarker analysis. Comparative Biochemistry and Physiology. Toxicology & Pharmacology, 227, 108626. google scholar
  • Cai, W., Yang, X., Li, X., Li, H., Wang, S., Wu, Z., Yu, M., Ma, S., & Tang, S. (2020). Low-dose Roundup induces developmental toxicity in bovine preimplantation embryos in vitro. Environmental Science and Pollution Research International, 27(14), 16451-16459. google scholar
  • Cao, Y., Gong, Y., Liu, L., Zhou, Y., Fang, X., Zhang, C., Li, Y., & Li, J. (2017). The use of human umbilical vein endothelial cells (HUVECs) as an in vitro model to assess the toxicity of nanoparticles to endo-thelium: a review. Journal of Applied Toxicology, 37(12), 1359-1369. google scholar
  • Deng, Y., Zhang, Y., Lu, Y., Zhao, Y., & Ren, H. (2016). Hepatotoxicity and nephrotoxicity induced by the chlorpyrifos and chlorpyrifos-methyl metabolite, 3,5,6-trichloro-2-pyridinol, in orally exposed mice”. The Science of the Total Environment, 544, 507-514. google scholar
  • Dokuyucu, R., Bilgili, A., Hanedan, B., Dogan, H., Dokuyucu, A., & Celik, M. M. (2016). Attenuating effects of caffeic acid phenethyl ester with intralipid on hepatotoxicity of chlorpyrifos in the case of rats. Medicina, 67(6), 743-749. google scholar
  • Ferreira, D., da Motta, A. C., Kreutz, L. C., Toni, C., Loro, V. L., & Barcel-los, L. J. (2010). Assessment of oxidative stress in Rhamdia quelen exposed to agrichemicals. Chemosphere, 79(9), 914-921. google scholar
  • Gholami-Seyedkolaei, S. J., Mirvaghefi, A., Farahmand, H., & Kosari, A. A. (2013). Effect of a glyphosate-based herbicide in Cyprinus carpio: assessment of acetylcholinesterase activity, hematologi-cal responses and serum biochemical parameters. Ecotoxicology and Environmental Safety, 98, 135-141. google scholar
  • Gündüz, E., Ülger, B. V., İbiloğlu, İ., Ekinci, A., Dursun, R., Zengin, Y., İçer, M., Uslukaya, Ö., Ekinci, C., & Güloğlu, C. (2015). Glutamine provides effective protection against deltamethrin-induced acute hepatotoxicity in rats but not against nephrotoxicity. Medi-cal Science Monitor:International Medical Journal of Experimental and Clinical Research, 21, 1107-1114. google scholar
  • Hatami, M., Banaee, M., & Nematdoost Haghi, B. (2019). Sub-lethal toxicity of chlorpyrifos alone and in combination with polyethyl-ene glycol to common carp (Cyprinus carpio). Chemosphere, 219, 981-988. google scholar
  • Jovanovic, P., Zoric, L., Stefanovic, I., Dzunic, B., Djordjevic-Jocic, J., Radenkovic, M., & Jovanovic, M. (2010). Lactate dehydrogenase and oxidative stress activity in primary open-angle glaucoma aqueous humour. Bosnian Journal of Basic Medical Sciences, 10(1), 83-88. google scholar
  • Kim, K. H., Kabir, E., & Jahan, S. A. (2017). Exposure to pesticides and the associated human health effects. The Science of the Total Environment, 575, 525-535. google scholar
  • Kumar, A., Sharma, R., Rana, D., & Sharma, N. (2019). Protective Effect of Alpha-Tocopherol in Deltamethrin Induced Immunotoxicity, En-docrine, Metabolic & Immune Disorders - Drug Targets,19(2), 171-184. Laetz, C. A., Baldwin, D. H., Collier, T. K., Hebert, V., Stark, J. D., & Scholz, N. L. (2009). The synergistic toxicity of pesticide mixtures: implica-tions for risk assessment and the conservation of endangered Pa-cific salmon. Environmental Health Perspectives, 117(3), 348-353. google scholar
  • Li, M. H., Ruan, L. Y., Zhou, J. W., Fu, Y. H., Jiang, L., Zhao, H., & Wang, J. S. (2017). Metabolic profiling of goldfish (Carassius auratis) after long-term glyphosate-based herbicide exposure. Aquatic Toxicol-ogy (Amsterdam, Netherlands), 188, 159-169. google scholar
  • Li, S., Jiang, Y., Sun, Q., Coffin, S., Chen, L., Qiao, K., Gui, W., & Zhu, G. (2020). Tebuconazole induced oxidative stress related hepatotoxicity in adult and larval zebrafish (Danio rerio). Chemosphere, 241, 125129. Lu, Q., Sun, Y., Ares, I., Anadon, A., Martfnez, M., Martfnez-Larrana-ga, M. R., Yuan, Z., Wang, X., & Martfnez, M. A. (2019). Deltamethrin toxicity: A review of oxidative stress and metabolism. Environ-mental Research, 170, 260-281. google scholar
  • Mansour, S. A., & Mossa, A. T. (2011). Adverse effects of exposure to low doses of chlorpyrifos in lactating rats. Toxicology and Indus-trial Health, 27(3), 213-224. google scholar
  • Martfnez, M. A., Rodrfguez, J. L., Lopez-Torres, B., Martfnez, M., Martrnez-Larranaga, M. R., Maximiliano, J. E., Anadon, A., & Ares, I. (2020). Use of human neuroblastoma SH-SY5Y cells to evaluate glyphosate-induced effects on oxidative stress, neuronal devel-opment and cell death signaling pathways. Environment Interna-tional, 135, 105414. google scholar
  • Medina-Leyte, D. J., Dommguez-Perez, M., Mercado, I., Villarreal-Molina, M.T., & Jacobo-Albavera, L. (2020). Use of human umbilical vein endothelial cells (HUVEC) as a model to study cardiovascular disease: A review. Applied Sciences, 10(3), 938. google scholar
  • Nasr, H. M., El-Demerdash, F. M., & El-Nagar, W. A. (2016). Neuro and renal toxicity induced by chlorpyrifos and abamectin in rats: Toxicity of insecticide mixture. Environmental Science and Pollu-tion Research International, 23(2), 1852-1859. google scholar
  • Ncir, M., Ben Salah, G., Kamoun, H., Makni Ayadi, F., Khabir, A., El Feki, A., & Saoudi, M. (2016). Histopathological, oxidative damage, biochemical, and genotoxicity alterations in hepatic rats exposed to deltamethrin: modulatory effects of garlic (Allium sativum). Canadian journal of Physiology and Pharmacology, 94(6), 571-578. Ndonwi, E. N., Atogho-Tiedeu, B., Lontchi-Yimagou, E., Shinkafi, T. S., Nanfa, D., Balti, E. V., Indusmita, R., Mahmood, A., Katte, J. C., Mbanya, A., Matsha, T., Mbanya, J. C., Shakir, & A., Sobngwi, E. (2019). Gestational Exposure to Pesticides Induces Oxidative Stress and Lipid Peroxidation in Offspring that Persist at Adult Age in an Animal Model. Toxicological Research, 35(3), 241-248. google scholar
  • Odetti, L. M., Lopez Gonzalez, E. C., Romito, M. L., Simoniello, M. F., & Poletta, G. L. (2020). Genotoxicity and oxidative stress in Caiman latirostris hatchlings exposed to pesticide formulations and their mixtures during incubation period. Ecotoxicology and Environ-mental Safety, 193, 110312. google scholar
  • Olsvik, P, A., Berntssen, M., & S0fteland, L. (2017). In vitro toxicity of pirimiphos-methyl in Atlantic salmon hepatocytes. Toxicology in Vitro, 39, 1-14. google scholar
  • Oyesola, T., Iranloye, B., & Adegoke, O. (2019). Implantation and pregnancy outcome of Sprague-Dawley rats exposed to pirimi-phos-methyl. Endocrine Regulations, 53(3), 139-145. google scholar
  • Piatti, E., Marabini, L., & Chiesara, E. (1994). Increase of micronu-cleus frequency in cultured rat hepatocytes treated in vitro with benomyl and pirimiphos-methyl separately and in mixture. Muta-tion Research, 324(1-2), 59-64. google scholar
  • Roelofs, M., Temming, A. R., Piersma, A. H., van den Berg, M., & van Duursen, M. (2014). Conazole fungicides inhibit Leydig cell testosterone secretion and androgen receptor activation in vitro. Toxicology Reports, 1, 271-283. google scholar
  • Sai, L., Li, X., Liu, Y., Guo, Q., Xie, L., Yu, G., Bo, C., Zhang, Z., & Li, L. (2014). Effects of chlorpyrifos on reproductive toxicology of male rats. Environmental Toxicology, 29(9), 1083-1088. google scholar
  • Staal, Y., Meijer, J., van der Kris, R., de Bruijn, A. C., Boersma, A. Y., Gremmer, E. R., Zwart, E. P., Beekhof, P. K., Slob, W., & van der Ven, L. (2018). Head skeleton malformations in zebrafish (Danio rerio) to assess adverse effects of mixtures of compounds. Archives of Toxicology, 92(12), 3549-3564 google scholar
  • Tilak, K. S., Veeraiah, K., & Rao, D. K. (2005). Biochemical changes induced by chlorpyrifos, an organophosphate compound in sub-lethal concentrations to the freshwater fish Catla catla, Labeo ro-hita and Cirrhinus mrigala. Journal of Environmental Biology, 26(2 Suppl), 341-347. google scholar
  • Velki, M., & Hackenberger, B. K. (2013). Different sensitivities of biomarker responses in two epigeic earthworm species after ex-posure to pyrethroid and organophosphate insecticides. Archives of Environmental Contamination and Toxicology, 65(3), 498-509. google scholar
  • Vijitharan, V., Warnasekare, J., Lokunarangoda, N. C., Farah, M. F., & Siribaddana, S. H. (2016). Fatal poisoning with plant growth regu-lator - chlormequat. The Ceylon Medical Journal, 61(2), 89-90. google scholar
  • Wang, X., Shen, M., Zhou, J., & Jin, Y. (2019). Chlorpyrifos disturbs hepatic metabolism associated with oxidative stress and gut mi-crobiota dysbiosis in adult zebrafish. Comparative Biochemistry and Physiology. Toxicology & Pharmacology, 216, 19-28. google scholar
  • Xiagedeer, B., Wu, S., Liu, Y., & Hao, W. (2016). Chlormequat chloride retards rat embryo growth in vitro. Toxicology in Vitro, 34, 274-282. google scholar
  • Yang, J., Gong, Y., Cai, J., Zheng, Y., Liu, H., & Zhang, Z. (2020). “Chlorpyrifos induces redox imbalance-dependent inflammation in common carp lymphocyte through dysfunction of T-cell re-ceptor y". Journal of Fish Diseases, 43(4), 423-430. google scholar
  • Yıldırım, E., Baydan, E., Kanbur, M., Kul, O., Cınar, M., Ekici, H., & Atmaca, N. (2013). “The effect of chlorpyrifos on isolated thoracic aorta in rats." BioMed Research International, 2013, 376051. google scholar
  • Zaki, S. M., Algaleel, W., Imam, R. A., Soliman, G. F., & Ghoneim, F. M. (2020). Nano-curcumin versus curcumin in amelioration of deltamethrin-induced hippocampal damage. Histochemistry and Cell Biology, 154(2), 157-175 google scholar
  • Zhang, H. C., Yang, Y. J., Ma, K. X., Shi, C. Y., Chen, G. W., & Liu, D. Z. (2020). “A novel sigma class glutathione S-transferase gene in freshwater planarian Dugesia japonica: cloning, characterization and protective effects in herbicide glyphosate stress". Ecotoxicol-ogy (London, England), 29(3), 295-304. google scholar

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APA

Sevim, Ç., Taghizadehghalehjoughi, A., & Kara, M. (2021). Effects of chlorpyrifos-methyl, chlormequat, deltamethrin, glyphosate, pirimiphos-methyl, tebuconazole and their mixture on oxidative stress and toxicity in HUVEC cell line. İstanbul Journal of Pharmacy, 51(2), 183-190. https://doi.org/10.26650/IstanbulJPharm.2021.881724


AMA

Sevim Ç, Taghizadehghalehjoughi A, Kara M. Effects of chlorpyrifos-methyl, chlormequat, deltamethrin, glyphosate, pirimiphos-methyl, tebuconazole and their mixture on oxidative stress and toxicity in HUVEC cell line. İstanbul Journal of Pharmacy. 2021;51(2):183-190. https://doi.org/10.26650/IstanbulJPharm.2021.881724


ABNT

Sevim, Ç.; Taghizadehghalehjoughi, A.; Kara, M. Effects of chlorpyrifos-methyl, chlormequat, deltamethrin, glyphosate, pirimiphos-methyl, tebuconazole and their mixture on oxidative stress and toxicity in HUVEC cell line. İstanbul Journal of Pharmacy, [Publisher Location], v. 51, n. 2, p. 183-190, 2021.


Chicago: Author-Date Style

Sevim, Çiğdem, and Ali Taghizadehghalehjoughi and Mehtap Kara. 2021. “Effects of chlorpyrifos-methyl, chlormequat, deltamethrin, glyphosate, pirimiphos-methyl, tebuconazole and their mixture on oxidative stress and toxicity in HUVEC cell line.” İstanbul Journal of Pharmacy 51, no. 2: 183-190. https://doi.org/10.26650/IstanbulJPharm.2021.881724


Chicago: Humanities Style

Sevim, Çiğdem, and Ali Taghizadehghalehjoughi and Mehtap Kara. Effects of chlorpyrifos-methyl, chlormequat, deltamethrin, glyphosate, pirimiphos-methyl, tebuconazole and their mixture on oxidative stress and toxicity in HUVEC cell line.” İstanbul Journal of Pharmacy 51, no. 2 (Sep. 2021): 183-190. https://doi.org/10.26650/IstanbulJPharm.2021.881724


Harvard: Australian Style

Sevim, Ç & Taghizadehghalehjoughi, A & Kara, M 2021, 'Effects of chlorpyrifos-methyl, chlormequat, deltamethrin, glyphosate, pirimiphos-methyl, tebuconazole and their mixture on oxidative stress and toxicity in HUVEC cell line', İstanbul Journal of Pharmacy, vol. 51, no. 2, pp. 183-190, viewed 24 Sep. 2021, https://doi.org/10.26650/IstanbulJPharm.2021.881724


Harvard: Author-Date Style

Sevim, Ç. and Taghizadehghalehjoughi, A. and Kara, M. (2021) ‘Effects of chlorpyrifos-methyl, chlormequat, deltamethrin, glyphosate, pirimiphos-methyl, tebuconazole and their mixture on oxidative stress and toxicity in HUVEC cell line’, İstanbul Journal of Pharmacy, 51(2), pp. 183-190. https://doi.org/10.26650/IstanbulJPharm.2021.881724 (24 Sep. 2021).


MLA

Sevim, Çiğdem, and Ali Taghizadehghalehjoughi and Mehtap Kara. Effects of chlorpyrifos-methyl, chlormequat, deltamethrin, glyphosate, pirimiphos-methyl, tebuconazole and their mixture on oxidative stress and toxicity in HUVEC cell line.” İstanbul Journal of Pharmacy, vol. 51, no. 2, 2021, pp. 183-190. [Database Container], https://doi.org/10.26650/IstanbulJPharm.2021.881724


Vancouver

Sevim Ç, Taghizadehghalehjoughi A, Kara M. Effects of chlorpyrifos-methyl, chlormequat, deltamethrin, glyphosate, pirimiphos-methyl, tebuconazole and their mixture on oxidative stress and toxicity in HUVEC cell line. İstanbul Journal of Pharmacy [Internet]. 24 Sep. 2021 [cited 24 Sep. 2021];51(2):183-190. Available from: https://doi.org/10.26650/IstanbulJPharm.2021.881724 doi: 10.26650/IstanbulJPharm.2021.881724


ISNAD

Sevim, Çiğdem - Taghizadehghalehjoughi, Ali - Kara, Mehtap. Effects of chlorpyrifos-methyl, chlormequat, deltamethrin, glyphosate, pirimiphos-methyl, tebuconazole and their mixture on oxidative stress and toxicity in HUVEC cell line”. İstanbul Journal of Pharmacy 51/2 (Sep. 2021): 183-190. https://doi.org/10.26650/IstanbulJPharm.2021.881724



TIMELINE


Submitted16.02.2021
Accepted09.05.2021
Published Online31.08.2021

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