Research Article


DOI :10.26650/IstanbulJPharm.2019.19058   IUP :10.26650/IstanbulJPharm.2019.19058    Full Text (PDF)

The subcronic effects of acetamipride on the global DNA methylation levels in Sprague-Dawley rat brain and liver

Sibel ÖzdenYağmur Emre ArıcanEcem Fatma Karaman

Acetamiprid, which is a neonicotinoid insecticide, is used to control leafy vegetables, fruiting vegetables, fir seeds, citrus fruits, pome fruits, grapes, cotton and ornamental plants and absorbent insects on flowers. The present study aim to evaluate global DNA methylation and gene expression of DNA methylation related enzymes in liver and brain tissues of male Sprague-Dawley rats after a 90-day subchronic exposure to acetamiprid at low doses of 12.5, 25 and 35 mg/kg body weight (b.w.). Global DNA methylation resulted in a significant decrease in the levels of 5-methylcytosine (5-mC%) at the doses of 25 and 35 mg/kg b.w. in the liver and 35 mg/kg b.w. in the brain compared to the vehicle control group. Consistently, expression of DNA methyltransferase enzymes decreased at doses of 12.5, 25 and 35 mg/kg b.w. in liver and 35 mg/kg b.w. in brain. It has been suggested that non-genotoxic (epigenetic) mechanisms may be involved in the toxicity of acetamiprid and further investigations are needed to elucidate the epigenetic effects of neonicotinoid insecticides.


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APA

Özden, S., Arıcan, Y., & Karaman, E. (2019). The subcronic effects of acetamipride on the global DNA methylation levels in Sprague-Dawley rat brain and liver. İstanbul Journal of Pharmacy, 49(3), 167-172. https://doi.org/10.26650/IstanbulJPharm.2019.19058


AMA

Özden S, Arıcan Y, Karaman E. The subcronic effects of acetamipride on the global DNA methylation levels in Sprague-Dawley rat brain and liver. İstanbul Journal of Pharmacy. 2019;49(3):167-172. https://doi.org/10.26650/IstanbulJPharm.2019.19058


ABNT

Özden, S.; Arıcan, Y.; Karaman, E. The subcronic effects of acetamipride on the global DNA methylation levels in Sprague-Dawley rat brain and liver. İstanbul Journal of Pharmacy, [Publisher Location], v. 49, n. 3, p. 167-172, 2019.


Chicago: Author-Date Style

Özden, Sibel, and Yağmur Emre Arıcan and Ecem Fatma Karaman. 2019. “The subcronic effects of acetamipride on the global DNA methylation levels in Sprague-Dawley rat brain and liver.” İstanbul Journal of Pharmacy 49, no. 3: 167-172. https://doi.org/10.26650/IstanbulJPharm.2019.19058


Chicago: Humanities Style

Özden, Sibel, and Yağmur Emre Arıcan and Ecem Fatma Karaman. The subcronic effects of acetamipride on the global DNA methylation levels in Sprague-Dawley rat brain and liver.” İstanbul Journal of Pharmacy 49, no. 3 (Sep. 2021): 167-172. https://doi.org/10.26650/IstanbulJPharm.2019.19058


Harvard: Australian Style

Özden, S & Arıcan, Y & Karaman, E 2019, 'The subcronic effects of acetamipride on the global DNA methylation levels in Sprague-Dawley rat brain and liver', İstanbul Journal of Pharmacy, vol. 49, no. 3, pp. 167-172, viewed 16 Sep. 2021, https://doi.org/10.26650/IstanbulJPharm.2019.19058


Harvard: Author-Date Style

Özden, S. and Arıcan, Y. and Karaman, E. (2019) ‘The subcronic effects of acetamipride on the global DNA methylation levels in Sprague-Dawley rat brain and liver’, İstanbul Journal of Pharmacy, 49(3), pp. 167-172. https://doi.org/10.26650/IstanbulJPharm.2019.19058 (16 Sep. 2021).


MLA

Özden, Sibel, and Yağmur Emre Arıcan and Ecem Fatma Karaman. The subcronic effects of acetamipride on the global DNA methylation levels in Sprague-Dawley rat brain and liver.” İstanbul Journal of Pharmacy, vol. 49, no. 3, 2019, pp. 167-172. [Database Container], https://doi.org/10.26650/IstanbulJPharm.2019.19058


Vancouver

Özden S, Arıcan Y, Karaman E. The subcronic effects of acetamipride on the global DNA methylation levels in Sprague-Dawley rat brain and liver. İstanbul Journal of Pharmacy [Internet]. 16 Sep. 2021 [cited 16 Sep. 2021];49(3):167-172. Available from: https://doi.org/10.26650/IstanbulJPharm.2019.19058 doi: 10.26650/IstanbulJPharm.2019.19058


ISNAD

Özden, Sibel - Arıcan, Yağmur Emre - Karaman, Ecem Fatma. The subcronic effects of acetamipride on the global DNA methylation levels in Sprague-Dawley rat brain and liver”. İstanbul Journal of Pharmacy 49/3 (Sep. 2021): 167-172. https://doi.org/10.26650/IstanbulJPharm.2019.19058



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Accepted07.11.2019

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