Research Article


DOI :10.26650/JARHS2022-1130823   IUP :10.26650/JARHS2022-1130823    Full Text (PDF)

CHARACTERIZATION OF MINOR HISTOCOMPATIBILITY ANTIGENS AND THEIR ROLE IN ALLOGENEIC HEMATOPOIETIC STEM CELL TRANSPLANTATION: SINGLE CENTER EXPERIENCE IN TURKIYE

Fatma Savran OğuzÇiğdem Kekik ÇınarSüleyman Rüştü OğuzDemet KıvançFatma Deniz SargınSevgi Beşışık Kalayoğlu

Objective: The minor histocompatibility antigens (mHAgs) are the epitopes composed of polymorphic essential peptides, and they create T cell response limited to a variety of class I and II HLA alleles. In recent years, there has been extensive research on the distribution of polymorphic regions in different populations. The incompatibility between recipient and donor, may initiate a strong cellular immune response despite HLA full-matched transplantation. We determined the frequency of minor antigens among hematopoietic stem cell transplantation (HSCT) recipients who underwent transplantation for various hematological diseases. Material and Methods: The study population included 200 healthy individuals, 150 HLA-typed patients who were candidates for allogeneic HSCT, and 20 recipients/donors with allogeneic transplants. Minor HAgs identified by using polymerase chain reaction (PCR) and sequence specific primer (SSP) methods. Results: When the allele frequencies and genotypes of the patients were compared with those of the healthy group, the difference was not significant regarding to immunogenic or non-immunogenic allele frequencies. The individuals with immunogenic homozygous H allele (HH genotype) were a few more in the healthy group, and this difference proved to be statistically significant. In fact, our study population Insufficient though the number was, based on the data received from 20 transplant patients and donors, GvHD was observed in 5 of 10 patients who had minor incompatibility. Conclusion: We assume that determining the mHAg frequencies in the healthy Turkish population and in patients with malignant hematological diseases will likely contribute to the understanding of the immune response.

DOI :10.26650/JARHS2022-1130823   IUP :10.26650/JARHS2022-1130823    Full Text (PDF)

MİNÖR DOKU UYGUNLUK ANTİJENLERİNİN KARAKTERİZASYONU VE ALLOJENİK HEMATOPOETİK KÖK HÜCRE NAKLİNDEKİ ROLÜ: TÜRKİYE’DE TEK MERKEZ DENEYİMİ

Fatma Savran OğuzÇiğdem Kekik ÇınarSüleyman Rüştü OğuzDemet KıvançFatma Deniz SargınSevgi Beşışık Kalayoğlu

Amaç: Minör doku uygunluk antijenleri (mHAg), polimorfik esansiyel peptitlerden oluşan epitoplar olup sınıf I ve II HLA allele çeşitleri ile sınırlı olan bir T hücresi yanıtı ortaya çıkarırlar. Son yıllarda, farklı popülasyonlarda polimorfik bölgelerin dağılımı hakkında kapsamlı araştırmalar yapılmıştır. Alıcı ve verici arasındaki mHAg uyumsuzluğu, HLA tam uyumlu transplantasyona rağmen güçlü bir hücresel immün yanıtı başlatabilir. Çalışmamızda çeşitli hematolojik hastalıklar nedeniyle hematopoietik kök hücre transplantasyonu (AKHN) yapılan hastalarda mHAg sıklığının belirlenmesi amaçlanmıştır. Gereç ve Yöntem: Çalışma popülasyonu 200 sağlıklı bireyi, allojenik AKHN adayları olarak HLA’sı tiplendirilmiş olan 150 hastayı ve 20 allojenik AKHN alıcısı/donörünü içermektedir. mHAg’lar, polimeraz zincir reaksiyonu (PZR) ve diziye özgü primer (SSP) yöntemleri kullanılarak belirlendi. Bulgular: Hastaların alel frekansları ve genotipleri sağlıklı grup ile karşılaştırıldığında, immünojenik veya immünojenik olmayan alel frekansları açısından fark anlamlı değildi. Sağlıklı grupta immünojenik homozigot H aleli (HH genotipi) olan bireyler daha azdı ve bu fark istatistiksel olarak anlamlıydı. Çalışma popülasyonumuz küçük olmasına rağmen, 20 transplant hastasından ve donöründen alınan verilere göre, 10 hastanın %50’sinin minör uyumsuzluk kaynaklı Graft versus host hastalığı (GVHH) olduğu gözlendi. Sonuç: Sağlıklı Türk popülasyonunda ve malign hematolojik hastalığı olan hastalarda mHAg frekanslarının belirlenmesinin immün yanıtın anlaşılmasına katkı sağlayacağını düşünmekteyiz.


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APA

Savran Oğuz, F., Kekik Çınar, Ç., Oğuz, S.R., Kıvanç, D., Sargın, F.D., & Beşışık Kalayoğlu, S. (2022). CHARACTERIZATION OF MINOR HISTOCOMPATIBILITY ANTIGENS AND THEIR ROLE IN ALLOGENEIC HEMATOPOIETIC STEM CELL TRANSPLANTATION: SINGLE CENTER EXPERIENCE IN TURKIYE. Journal of Advanced Research in Health Sciences, 5(3), 130-134. https://doi.org/10.26650/JARHS2022-1130823


AMA

Savran Oğuz F, Kekik Çınar Ç, Oğuz S R, Kıvanç D, Sargın F D, Beşışık Kalayoğlu S. CHARACTERIZATION OF MINOR HISTOCOMPATIBILITY ANTIGENS AND THEIR ROLE IN ALLOGENEIC HEMATOPOIETIC STEM CELL TRANSPLANTATION: SINGLE CENTER EXPERIENCE IN TURKIYE. Journal of Advanced Research in Health Sciences. 2022;5(3):130-134. https://doi.org/10.26650/JARHS2022-1130823


ABNT

Savran Oğuz, F.; Kekik Çınar, Ç.; Oğuz, S.R.; Kıvanç, D.; Sargın, F.D.; Beşışık Kalayoğlu, S. CHARACTERIZATION OF MINOR HISTOCOMPATIBILITY ANTIGENS AND THEIR ROLE IN ALLOGENEIC HEMATOPOIETIC STEM CELL TRANSPLANTATION: SINGLE CENTER EXPERIENCE IN TURKIYE. Journal of Advanced Research in Health Sciences, [Publisher Location], v. 5, n. 3, p. 130-134, 2022.


Chicago: Author-Date Style

Savran Oğuz, Fatma, and Çiğdem Kekik Çınar and Süleyman Rüştü Oğuz and Demet Kıvanç and Fatma Deniz Sargın and Sevgi Beşışık Kalayoğlu. 2022. “CHARACTERIZATION OF MINOR HISTOCOMPATIBILITY ANTIGENS AND THEIR ROLE IN ALLOGENEIC HEMATOPOIETIC STEM CELL TRANSPLANTATION: SINGLE CENTER EXPERIENCE IN TURKIYE.” Journal of Advanced Research in Health Sciences 5, no. 3: 130-134. https://doi.org/10.26650/JARHS2022-1130823


Chicago: Humanities Style

Savran Oğuz, Fatma, and Çiğdem Kekik Çınar and Süleyman Rüştü Oğuz and Demet Kıvanç and Fatma Deniz Sargın and Sevgi Beşışık Kalayoğlu. CHARACTERIZATION OF MINOR HISTOCOMPATIBILITY ANTIGENS AND THEIR ROLE IN ALLOGENEIC HEMATOPOIETIC STEM CELL TRANSPLANTATION: SINGLE CENTER EXPERIENCE IN TURKIYE.” Journal of Advanced Research in Health Sciences 5, no. 3 (Dec. 2022): 130-134. https://doi.org/10.26650/JARHS2022-1130823


Harvard: Australian Style

Savran Oğuz, F & Kekik Çınar, Ç & Oğuz, SR & Kıvanç, D & Sargın, FD & Beşışık Kalayoğlu, S 2022, 'CHARACTERIZATION OF MINOR HISTOCOMPATIBILITY ANTIGENS AND THEIR ROLE IN ALLOGENEIC HEMATOPOIETIC STEM CELL TRANSPLANTATION: SINGLE CENTER EXPERIENCE IN TURKIYE', Journal of Advanced Research in Health Sciences, vol. 5, no. 3, pp. 130-134, viewed 4 Dec. 2022, https://doi.org/10.26650/JARHS2022-1130823


Harvard: Author-Date Style

Savran Oğuz, F. and Kekik Çınar, Ç. and Oğuz, S.R. and Kıvanç, D. and Sargın, F.D. and Beşışık Kalayoğlu, S. (2022) ‘CHARACTERIZATION OF MINOR HISTOCOMPATIBILITY ANTIGENS AND THEIR ROLE IN ALLOGENEIC HEMATOPOIETIC STEM CELL TRANSPLANTATION: SINGLE CENTER EXPERIENCE IN TURKIYE’, Journal of Advanced Research in Health Sciences, 5(3), pp. 130-134. https://doi.org/10.26650/JARHS2022-1130823 (4 Dec. 2022).


MLA

Savran Oğuz, Fatma, and Çiğdem Kekik Çınar and Süleyman Rüştü Oğuz and Demet Kıvanç and Fatma Deniz Sargın and Sevgi Beşışık Kalayoğlu. CHARACTERIZATION OF MINOR HISTOCOMPATIBILITY ANTIGENS AND THEIR ROLE IN ALLOGENEIC HEMATOPOIETIC STEM CELL TRANSPLANTATION: SINGLE CENTER EXPERIENCE IN TURKIYE.” Journal of Advanced Research in Health Sciences, vol. 5, no. 3, 2022, pp. 130-134. [Database Container], https://doi.org/10.26650/JARHS2022-1130823


Vancouver

Savran Oğuz F, Kekik Çınar Ç, Oğuz SR, Kıvanç D, Sargın FD, Beşışık Kalayoğlu S. CHARACTERIZATION OF MINOR HISTOCOMPATIBILITY ANTIGENS AND THEIR ROLE IN ALLOGENEIC HEMATOPOIETIC STEM CELL TRANSPLANTATION: SINGLE CENTER EXPERIENCE IN TURKIYE. Journal of Advanced Research in Health Sciences [Internet]. 4 Dec. 2022 [cited 4 Dec. 2022];5(3):130-134. Available from: https://doi.org/10.26650/JARHS2022-1130823 doi: 10.26650/JARHS2022-1130823


ISNAD

Savran Oğuz, Fatma - Kekik Çınar, Çiğdem - Oğuz, SüleymanRüştü - Kıvanç, Demet - Sargın, FatmaDeniz - Beşışık Kalayoğlu, Sevgi. CHARACTERIZATION OF MINOR HISTOCOMPATIBILITY ANTIGENS AND THEIR ROLE IN ALLOGENEIC HEMATOPOIETIC STEM CELL TRANSPLANTATION: SINGLE CENTER EXPERIENCE IN TURKIYE”. Journal of Advanced Research in Health Sciences 5/3 (Dec. 2022): 130-134. https://doi.org/10.26650/JARHS2022-1130823



TIMELINE


Submitted16.06.2022
Accepted22.06.2022
Published Online29.07.2022

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