Meeting Abstract


DOI :10.26650/JARHS2021-1136760   IUP :10.26650/JARHS2021-1136760    Full Text (PDF)

EVALUATION OF EXOSOME-DERIVED CircRNA EXPRESSION PATTERNS IN LUNG CANCER RADIOTHERAPY

Saina RahimiMilad AsadiCan MüftüoğluEmin TavlayanÖzlem Özkaya AkagündüzUfuk MertAyşe Caner

Background: Radiotherapy is an effective method used in the treatment of lung cancer. However, it is very difficult to assess the clinical manifestations of radiotherapy at an early stage and the relevant criteria are very limited. Exosome-derived circRNAs (exo-circRNAs) have potential as biomarkers for early a cancer diagnosis and treatment assessment as exosomes are present in a variety of body fluids and circRNAs in exosomes exhibit high stability. Therefore, we aimed to investigate for potential biomarker candidates by evaluating changes in exo-circRNA expression with radiotherapy in lung cancer. Method: In this study, lung cancer epithelial (A549) and healthy lung fibroblast (WI38) cell lines were used. After colony assay, both cell lines were irradiated with 4 Gy and exosomes were isolated by ultracentrifugation method. Exosomes were characterized by flow cytometry and nanoparticle tracking analysis, and RNA isolation was performed by trizol protocol. Then, the expression levels of 40 exo-circRNAs in all groups were examined by RT-qPCR. Results: Of the exosomal circRNA investigated, 5 exo-circRNAs were found to be expressed in at least one of the groups, including A549, WI38, irradiated A549 and irradiated WI-38 cell lines. In particular, hsa_circ_0014235 and hsa_circ_0001900, which were found to be expressed in all groups, were more upregulated in cancer cell lines than healthy cell line. Significant changes were detected in the expression levels of these exo-circRNAs after irradiation. Conclusions: Differential expression of exo-circRNAs between cancer and healthy group and alterations by irradiation suggests that they may be potential diagnostic and monitoring biomarkers in cancer.


PDF View

References

    Citations

    Copy and paste a formatted citation or use one of the options to export in your chosen format


    EXPORT



    APA

    Rahimi, S., Asadi, M., Müftüoğlu, C., Tavlayan, E., Özkaya Akagündüz, Ö., Mert, U., & Caner, A. (2022). EVALUATION OF EXOSOME-DERIVED CircRNA EXPRESSION PATTERNS IN LUNG CANCER RADIOTHERAPY. Journal of Advanced Research in Health Sciences, 5(1), 51-51. https://doi.org/10.26650/JARHS2021-1136760


    AMA

    Rahimi S, Asadi M, Müftüoğlu C, Tavlayan E, Özkaya Akagündüz Ö, Mert U, Caner A. EVALUATION OF EXOSOME-DERIVED CircRNA EXPRESSION PATTERNS IN LUNG CANCER RADIOTHERAPY. Journal of Advanced Research in Health Sciences. 2022;5(1):51-51. https://doi.org/10.26650/JARHS2021-1136760


    ABNT

    Rahimi, S.; Asadi, M.; Müftüoğlu, C.; Tavlayan, E.; Özkaya Akagündüz, Ö.; Mert, U.; Caner, A. EVALUATION OF EXOSOME-DERIVED CircRNA EXPRESSION PATTERNS IN LUNG CANCER RADIOTHERAPY. Journal of Advanced Research in Health Sciences, [Publisher Location], v. 5, n. 1, p. 51-51, 2022.


    Chicago: Author-Date Style

    Rahimi, Saina, and Milad Asadi and Can Müftüoğlu and Emin Tavlayan and Özlem Özkaya Akagündüz and Ufuk Mert and Ayşe Caner. 2022. “EVALUATION OF EXOSOME-DERIVED CircRNA EXPRESSION PATTERNS IN LUNG CANCER RADIOTHERAPY.” Journal of Advanced Research in Health Sciences 5, no. 1: 51-51. https://doi.org/10.26650/JARHS2021-1136760


    Chicago: Humanities Style

    Rahimi, Saina, and Milad Asadi and Can Müftüoğlu and Emin Tavlayan and Özlem Özkaya Akagündüz and Ufuk Mert and Ayşe Caner. EVALUATION OF EXOSOME-DERIVED CircRNA EXPRESSION PATTERNS IN LUNG CANCER RADIOTHERAPY.” Journal of Advanced Research in Health Sciences 5, no. 1 (Feb. 2024): 51-51. https://doi.org/10.26650/JARHS2021-1136760


    Harvard: Australian Style

    Rahimi, S & Asadi, M & Müftüoğlu, C & Tavlayan, E & Özkaya Akagündüz, Ö & Mert, U & Caner, A 2022, 'EVALUATION OF EXOSOME-DERIVED CircRNA EXPRESSION PATTERNS IN LUNG CANCER RADIOTHERAPY', Journal of Advanced Research in Health Sciences, vol. 5, no. 1, pp. 51-51, viewed 24 Feb. 2024, https://doi.org/10.26650/JARHS2021-1136760


    Harvard: Author-Date Style

    Rahimi, S. and Asadi, M. and Müftüoğlu, C. and Tavlayan, E. and Özkaya Akagündüz, Ö. and Mert, U. and Caner, A. (2022) ‘EVALUATION OF EXOSOME-DERIVED CircRNA EXPRESSION PATTERNS IN LUNG CANCER RADIOTHERAPY’, Journal of Advanced Research in Health Sciences, 5(1), pp. 51-51. https://doi.org/10.26650/JARHS2021-1136760 (24 Feb. 2024).


    MLA

    Rahimi, Saina, and Milad Asadi and Can Müftüoğlu and Emin Tavlayan and Özlem Özkaya Akagündüz and Ufuk Mert and Ayşe Caner. EVALUATION OF EXOSOME-DERIVED CircRNA EXPRESSION PATTERNS IN LUNG CANCER RADIOTHERAPY.” Journal of Advanced Research in Health Sciences, vol. 5, no. 1, 2022, pp. 51-51. [Database Container], https://doi.org/10.26650/JARHS2021-1136760


    Vancouver

    Rahimi S, Asadi M, Müftüoğlu C, Tavlayan E, Özkaya Akagündüz Ö, Mert U, Caner A. EVALUATION OF EXOSOME-DERIVED CircRNA EXPRESSION PATTERNS IN LUNG CANCER RADIOTHERAPY. Journal of Advanced Research in Health Sciences [Internet]. 24 Feb. 2024 [cited 24 Feb. 2024];5(1):51-51. Available from: https://doi.org/10.26650/JARHS2021-1136760 doi: 10.26650/JARHS2021-1136760


    ISNAD

    Rahimi, Saina - Asadi, Milad - Müftüoğlu, Can - Tavlayan, Emin - Özkaya Akagündüz, Özlem - Mert, Ufuk - Caner, Ayşe. EVALUATION OF EXOSOME-DERIVED CircRNA EXPRESSION PATTERNS IN LUNG CANCER RADIOTHERAPY”. Journal of Advanced Research in Health Sciences 5/1 (Feb. 2024): 51-51. https://doi.org/10.26650/JARHS2021-1136760



    TIMELINE


    Published Online09.08.2022

    LICENCE


    Attribution-NonCommercial (CC BY-NC)

    This license lets others remix, tweak, and build upon your work non-commercially, and although their new works must also acknowledge you and be non-commercial, they don’t have to license their derivative works on the same terms.


    SHARE




    Istanbul University Press aims to contribute to the dissemination of ever growing scientific knowledge through publication of high quality scientific journals and books in accordance with the international publishing standards and ethics. Istanbul University Press follows an open access, non-commercial, scholarly publishing.