Meeting Abstract


DOI :10.26650/JARHS2021-1138086   IUP :10.26650/JARHS2021-1138086    Full Text (PDF)

METHYLATION AND EXPRESSION VALIDATION RESULTS OF GPRC5C GENE OBTAINED FROM EPIGENOMICS AND TRANSCRIPTOMICS DATA IN ORAL CAVITY TUMORS

Gizem EsentürkCan DorukMurat UlusanVakur OlgaçSemra Demokan

Objective: Demokan et.al idetified G/Protein/Coupled/Receptor/Class-C/Group-5/Member-C (GPRC5C) as a candidate gene in OSCC via expression and methylation array results (logFC:0.59663; DeltaBeta: -0.130984561) from their previous unpublished data. In our study, the methylation and expression status of GPRC5C gene was investigated in a larger group of OSCC patients and healthy cohort for further validation. Its potential candidate biomarker was evaluated for early diagnosis of OSCC by non-invasive methods. Materials and Methods: DNA and RNA were isolated from tissues/body fluid samples of 30 patients OSCC and 15 healthy individuals. The methylation and expression levels were analyzed by using QMSP and QRT-PCR methods, respectively. The data were compared with clinical parameters and evaluated by statistical analysis. Results: Decreased expression levels were observed in 43% and increased expression levels were observed in 33% in the tumor tissues compared to corresponding-normal tissues of OSCC patients. Methylation-based expression loss was observed in 4 patients having the ratio between 3.4 and 12.2%. We also observed a decrease in expression in OSCC patients with observed methylation. There was statistical significance between differentiation degree and expression in OSCC. Conclusion: It was concluded that GPRC5C may play a role as a potential methylation biomarker candidate of specific subgroups of the oral cavity. Other mechanisms need to be explored to understand the differences in expression levels.


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    APA

    Esentürk, G., Doruk, C., Ulusan, M., Olgaç, V., & Demokan, S. (2022). METHYLATION AND EXPRESSION VALIDATION RESULTS OF GPRC5C GENE OBTAINED FROM EPIGENOMICS AND TRANSCRIPTOMICS DATA IN ORAL CAVITY TUMORS. Journal of Advanced Research in Health Sciences, 5(1), 46-46. https://doi.org/10.26650/JARHS2021-1138086


    AMA

    Esentürk G, Doruk C, Ulusan M, Olgaç V, Demokan S. METHYLATION AND EXPRESSION VALIDATION RESULTS OF GPRC5C GENE OBTAINED FROM EPIGENOMICS AND TRANSCRIPTOMICS DATA IN ORAL CAVITY TUMORS. Journal of Advanced Research in Health Sciences. 2022;5(1):46-46. https://doi.org/10.26650/JARHS2021-1138086


    ABNT

    Esentürk, G.; Doruk, C.; Ulusan, M.; Olgaç, V.; Demokan, S. METHYLATION AND EXPRESSION VALIDATION RESULTS OF GPRC5C GENE OBTAINED FROM EPIGENOMICS AND TRANSCRIPTOMICS DATA IN ORAL CAVITY TUMORS. Journal of Advanced Research in Health Sciences, [Publisher Location], v. 5, n. 1, p. 46-46, 2022.


    Chicago: Author-Date Style

    Esentürk, Gizem, and Can Doruk and Murat Ulusan and Vakur Olgaç and Semra Demokan. 2022. “METHYLATION AND EXPRESSION VALIDATION RESULTS OF GPRC5C GENE OBTAINED FROM EPIGENOMICS AND TRANSCRIPTOMICS DATA IN ORAL CAVITY TUMORS.” Journal of Advanced Research in Health Sciences 5, no. 1: 46-46. https://doi.org/10.26650/JARHS2021-1138086


    Chicago: Humanities Style

    Esentürk, Gizem, and Can Doruk and Murat Ulusan and Vakur Olgaç and Semra Demokan. METHYLATION AND EXPRESSION VALIDATION RESULTS OF GPRC5C GENE OBTAINED FROM EPIGENOMICS AND TRANSCRIPTOMICS DATA IN ORAL CAVITY TUMORS.” Journal of Advanced Research in Health Sciences 5, no. 1 (Feb. 2024): 46-46. https://doi.org/10.26650/JARHS2021-1138086


    Harvard: Australian Style

    Esentürk, G & Doruk, C & Ulusan, M & Olgaç, V & Demokan, S 2022, 'METHYLATION AND EXPRESSION VALIDATION RESULTS OF GPRC5C GENE OBTAINED FROM EPIGENOMICS AND TRANSCRIPTOMICS DATA IN ORAL CAVITY TUMORS', Journal of Advanced Research in Health Sciences, vol. 5, no. 1, pp. 46-46, viewed 24 Feb. 2024, https://doi.org/10.26650/JARHS2021-1138086


    Harvard: Author-Date Style

    Esentürk, G. and Doruk, C. and Ulusan, M. and Olgaç, V. and Demokan, S. (2022) ‘METHYLATION AND EXPRESSION VALIDATION RESULTS OF GPRC5C GENE OBTAINED FROM EPIGENOMICS AND TRANSCRIPTOMICS DATA IN ORAL CAVITY TUMORS’, Journal of Advanced Research in Health Sciences, 5(1), pp. 46-46. https://doi.org/10.26650/JARHS2021-1138086 (24 Feb. 2024).


    MLA

    Esentürk, Gizem, and Can Doruk and Murat Ulusan and Vakur Olgaç and Semra Demokan. METHYLATION AND EXPRESSION VALIDATION RESULTS OF GPRC5C GENE OBTAINED FROM EPIGENOMICS AND TRANSCRIPTOMICS DATA IN ORAL CAVITY TUMORS.” Journal of Advanced Research in Health Sciences, vol. 5, no. 1, 2022, pp. 46-46. [Database Container], https://doi.org/10.26650/JARHS2021-1138086


    Vancouver

    Esentürk G, Doruk C, Ulusan M, Olgaç V, Demokan S. METHYLATION AND EXPRESSION VALIDATION RESULTS OF GPRC5C GENE OBTAINED FROM EPIGENOMICS AND TRANSCRIPTOMICS DATA IN ORAL CAVITY TUMORS. Journal of Advanced Research in Health Sciences [Internet]. 24 Feb. 2024 [cited 24 Feb. 2024];5(1):46-46. Available from: https://doi.org/10.26650/JARHS2021-1138086 doi: 10.26650/JARHS2021-1138086


    ISNAD

    Esentürk, Gizem - Doruk, Can - Ulusan, Murat - Olgaç, Vakur - Demokan, Semra. METHYLATION AND EXPRESSION VALIDATION RESULTS OF GPRC5C GENE OBTAINED FROM EPIGENOMICS AND TRANSCRIPTOMICS DATA IN ORAL CAVITY TUMORS”. Journal of Advanced Research in Health Sciences 5/1 (Feb. 2024): 46-46. https://doi.org/10.26650/JARHS2021-1138086



    TIMELINE


    Published Online09.08.2022

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