Meeting Abstract


DOI :10.26650/JARHS2021-1135358   IUP :10.26650/JARHS2021-1135358    Full Text (PDF)

miRNA-REGULATED PATHWAYS OF CD8+ T CELLS IN TNBC MOUSE MODEL

Müge Öçal DemirtaşBala Gür Dedeoğlu

Objective: Triple negative breast cancer, which constitutes 20% of all breast cancer is the most aggressive breast cancer subtype. Although breast cancer is known as immune cold, recent studies have revealed that there is an immune response on the basis of subtypes. miRNAs are involved in regulating many biological processes of CD8+ T lymphocytes, which are adaptive immune system cells that effectively destroy tumor cells. Our aim is to examine the regulative roles of miRNAs in CD8+ T lymphocytes during the formation of triple negative breast tumors in mice. Materials and Methods:4T1 cells and Balb/c mice were used for the allograft breast cancer model. After 2 weeks, tumor-bearing and control mice were sacrificed, and spleens were harvested for CD8+ T cell isolation. Following RNA isolation, miRNA microarray analysis was performed. Differentially expressed miRNAs between the groups were detected. DIANA-mirPath web tool was used to investigate the target genes of miRNAs and in which pathways these genes are enriched. Results: Microarray analysis indicated that 41 miRNAs are differentially expressed in CD8+ T cells from tumor-bearing mice compared to the control group. The KEGG analysis results showed that the targets of differentially expressed miRNAs were significantly enriched in immune response-related pathways like ECM-receptor interaction, PI3K-Akt signaling, and tryptophan metabolism pathways. Conclusion: The pathways in which miRNA-target genes are enriched are associated with T cell metabolism and cell survival. It may be concluded that there are changes in the systemic adaptive immune response during TNBC formation and that miRNAs regulate these changes.


PDF View

References

    Citations

    Copy and paste a formatted citation or use one of the options to export in your chosen format


    EXPORT



    APA

    Öçal Demirtaş, M., & Gür Dedeoğlu, B. (2022). miRNA-REGULATED PATHWAYS OF CD8+ T CELLS IN TNBC MOUSE MODEL. Journal of Advanced Research in Health Sciences, 5(1), 58-58. https://doi.org/10.26650/JARHS2021-1135358


    AMA

    Öçal Demirtaş M, Gür Dedeoğlu B. miRNA-REGULATED PATHWAYS OF CD8+ T CELLS IN TNBC MOUSE MODEL. Journal of Advanced Research in Health Sciences. 2022;5(1):58-58. https://doi.org/10.26650/JARHS2021-1135358


    ABNT

    Öçal Demirtaş, M.; Gür Dedeoğlu, B. miRNA-REGULATED PATHWAYS OF CD8+ T CELLS IN TNBC MOUSE MODEL. Journal of Advanced Research in Health Sciences, [Publisher Location], v. 5, n. 1, p. 58-58, 2022.


    Chicago: Author-Date Style

    Öçal Demirtaş, Müge, and Bala Gür Dedeoğlu. 2022. “miRNA-REGULATED PATHWAYS OF CD8+ T CELLS IN TNBC MOUSE MODEL.” Journal of Advanced Research in Health Sciences 5, no. 1: 58-58. https://doi.org/10.26650/JARHS2021-1135358


    Chicago: Humanities Style

    Öçal Demirtaş, Müge, and Bala Gür Dedeoğlu. miRNA-REGULATED PATHWAYS OF CD8+ T CELLS IN TNBC MOUSE MODEL.” Journal of Advanced Research in Health Sciences 5, no. 1 (Feb. 2024): 58-58. https://doi.org/10.26650/JARHS2021-1135358


    Harvard: Australian Style

    Öçal Demirtaş, M & Gür Dedeoğlu, B 2022, 'miRNA-REGULATED PATHWAYS OF CD8+ T CELLS IN TNBC MOUSE MODEL', Journal of Advanced Research in Health Sciences, vol. 5, no. 1, pp. 58-58, viewed 24 Feb. 2024, https://doi.org/10.26650/JARHS2021-1135358


    Harvard: Author-Date Style

    Öçal Demirtaş, M. and Gür Dedeoğlu, B. (2022) ‘miRNA-REGULATED PATHWAYS OF CD8+ T CELLS IN TNBC MOUSE MODEL’, Journal of Advanced Research in Health Sciences, 5(1), pp. 58-58. https://doi.org/10.26650/JARHS2021-1135358 (24 Feb. 2024).


    MLA

    Öçal Demirtaş, Müge, and Bala Gür Dedeoğlu. miRNA-REGULATED PATHWAYS OF CD8+ T CELLS IN TNBC MOUSE MODEL.” Journal of Advanced Research in Health Sciences, vol. 5, no. 1, 2022, pp. 58-58. [Database Container], https://doi.org/10.26650/JARHS2021-1135358


    Vancouver

    Öçal Demirtaş M, Gür Dedeoğlu B. miRNA-REGULATED PATHWAYS OF CD8+ T CELLS IN TNBC MOUSE MODEL. Journal of Advanced Research in Health Sciences [Internet]. 24 Feb. 2024 [cited 24 Feb. 2024];5(1):58-58. Available from: https://doi.org/10.26650/JARHS2021-1135358 doi: 10.26650/JARHS2021-1135358


    ISNAD

    Öçal Demirtaş, Müge - Gür Dedeoğlu, Bala. miRNA-REGULATED PATHWAYS OF CD8+ T CELLS IN TNBC MOUSE MODEL”. Journal of Advanced Research in Health Sciences 5/1 (Feb. 2024): 58-58. https://doi.org/10.26650/JARHS2021-1135358



    TIMELINE


    Published Online09.08.2022

    LICENCE


    Attribution-NonCommercial (CC BY-NC)

    This license lets others remix, tweak, and build upon your work non-commercially, and although their new works must also acknowledge you and be non-commercial, they don’t have to license their derivative works on the same terms.


    SHARE




    Istanbul University Press aims to contribute to the dissemination of ever growing scientific knowledge through publication of high quality scientific journals and books in accordance with the international publishing standards and ethics. Istanbul University Press follows an open access, non-commercial, scholarly publishing.