Research Article


DOI :10.26650/JARHS2020-668235   IUP :10.26650/JARHS2020-668235    Full Text (PDF)

Efficiency of co-cultured peripheral blood mononuclear cells with primary parathyroid tissue cells

Sevde HasanoğluBeyza GöncüEmrah Yücesan

In organ failure, pre-transplant immunoassays are cost-effective and sensitive tests performed under the supervision of a specialist team. The aim of this study is to develop a more economical and easy to handle method that is relatively easy to perform in vitro with coculture models. Peripheral blood mononuclear cells from permenant hypoparathyroidism patients (n=4) were co-cultured with parathyroid cells of two different donors both with and without a barrier. Parathormone and cell counting were determined at 24 and 72 hours of cultivation. Parathormone levels were significantly increased in two separate parathyroid cell groups, whereas the cell number was significantly decreased in both groups. Peripheral blood mononuclear cells did not show a significant change against foreign parathyroid cells in both co-culture groups with barriers and without barriers when compared with the control group. In this study, the co-culture model, which is easier to manipulate than other sensitive tests, was performed. Basically, in order to determine the process of predicting the survival of the transplant which was carried out at a lower cost. However, the two separate co-culture models did not create a significant difference for parathyroid cells and peripheral blood mononuclear cells both in the presence of a barrier and without a barrier. In future studies, different methods should be used to experience prediction of survival by performing more sensitive tests. 

DOI :10.26650/JARHS2020-668235   IUP :10.26650/JARHS2020-668235    Full Text (PDF)

Periferik kan mononükleer hücrelerin primer paratiroit dokusu hücreleri ile ko-kültürünün verimliliği

Sevde HasanoğluBeyza GöncüEmrah Yücesan

Organ yetmezliğinde, nakil öncesi uygulanan immün testler ekonomik anlamda maliyetli ve uzman ekip gözetiminde uygulanan hassas testlerdir. Uygulaması görece daha kolay olan in vitro ko-kültür modelleri ile daha ekonomik ve uygulama kolaylığı olan bir yöntem geliştirilmesi hedeflenmiştir. Kalıcı hipoparatiroidi hastalarına ait periferik kan mononükleer hücreleri (n=4) ile iki farklı donöre ait paratiroit hücresinin ayırıcı bir bariyer olmadan ve bariyer kullanılarak ko-kültüre edilmesiyle bir model oluşturulmuştur. Parathormon ve hücre sayım takibi 24 ve 72. saatlerde gerçekleştirilmiştir. İki ayrı paratiroit hücre grubunda parathormon seviyesi anlamlı şekilde artarken, hücre sayısı anlamlı şekilde her iki grupta da azalmıştır. Kontrol grubu ile karşılaştırıldığında ise ayırıcı bariyer kullanıldığı ve kullanılmadığı her iki durumda da periferik kan mononükleer hücreleri yabancı paratiroit hücrelerine karşı anlamlı bir değişim göstermemiştir. Bu çalışmada uygulanan ko-kültür modeli, manipülasyonu diğer hassas testlere göre daha kolaydır. Temelde, nakil sağ kalımının öngörülmesi sürecinin daha az maliyetle belirlenebilmesi için gerçekleştirilmiştir. Fakat uygulanan iki ayrı ko-kültür modeli hem ayırıcı bir bariyer eşliğinde hem de ayırıcı bir bariyer olmadan paratiroit hücreleri ve PKMH için anlamlı bir farklılık oluşturmamıştır. İlerleyen çalışmalarda daha hassas testlerin uygulanarak sağ kalım öngörülmesi için farklı yöntemlerin denenmesi hedeflenmektedir. 


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APA

Hasanoğlu, S., Göncü, B., & Yücesan, E. (2020). Efficiency of co-cultured peripheral blood mononuclear cells with primary parathyroid tissue cells. Journal of Advanced Research in Health Sciences, 3(1), 5-12. https://doi.org/10.26650/JARHS2020-668235


AMA

Hasanoğlu S, Göncü B, Yücesan E. Efficiency of co-cultured peripheral blood mononuclear cells with primary parathyroid tissue cells. Journal of Advanced Research in Health Sciences. 2020;3(1):5-12. https://doi.org/10.26650/JARHS2020-668235


ABNT

Hasanoğlu, S.; Göncü, B.; Yücesan, E. Efficiency of co-cultured peripheral blood mononuclear cells with primary parathyroid tissue cells. Journal of Advanced Research in Health Sciences, [Publisher Location], v. 3, n. 1, p. 5-12, 2020.


Chicago: Author-Date Style

Hasanoğlu, Sevde, and Beyza Göncü and Emrah Yücesan. 2020. “Efficiency of co-cultured peripheral blood mononuclear cells with primary parathyroid tissue cells.” Journal of Advanced Research in Health Sciences 3, no. 1: 5-12. https://doi.org/10.26650/JARHS2020-668235


Chicago: Humanities Style

Hasanoğlu, Sevde, and Beyza Göncü and Emrah Yücesan. Efficiency of co-cultured peripheral blood mononuclear cells with primary parathyroid tissue cells.” Journal of Advanced Research in Health Sciences 3, no. 1 (Dec. 2021): 5-12. https://doi.org/10.26650/JARHS2020-668235


Harvard: Australian Style

Hasanoğlu, S & Göncü, B & Yücesan, E 2020, 'Efficiency of co-cultured peripheral blood mononuclear cells with primary parathyroid tissue cells', Journal of Advanced Research in Health Sciences, vol. 3, no. 1, pp. 5-12, viewed 9 Dec. 2021, https://doi.org/10.26650/JARHS2020-668235


Harvard: Author-Date Style

Hasanoğlu, S. and Göncü, B. and Yücesan, E. (2020) ‘Efficiency of co-cultured peripheral blood mononuclear cells with primary parathyroid tissue cells’, Journal of Advanced Research in Health Sciences, 3(1), pp. 5-12. https://doi.org/10.26650/JARHS2020-668235 (9 Dec. 2021).


MLA

Hasanoğlu, Sevde, and Beyza Göncü and Emrah Yücesan. Efficiency of co-cultured peripheral blood mononuclear cells with primary parathyroid tissue cells.” Journal of Advanced Research in Health Sciences, vol. 3, no. 1, 2020, pp. 5-12. [Database Container], https://doi.org/10.26650/JARHS2020-668235


Vancouver

Hasanoğlu S, Göncü B, Yücesan E. Efficiency of co-cultured peripheral blood mononuclear cells with primary parathyroid tissue cells. Journal of Advanced Research in Health Sciences [Internet]. 9 Dec. 2021 [cited 9 Dec. 2021];3(1):5-12. Available from: https://doi.org/10.26650/JARHS2020-668235 doi: 10.26650/JARHS2020-668235


ISNAD

Hasanoğlu, Sevde - Göncü, Beyza - Yücesan, Emrah. Efficiency of co-cultured peripheral blood mononuclear cells with primary parathyroid tissue cells”. Journal of Advanced Research in Health Sciences 3/1 (Dec. 2021): 5-12. https://doi.org/10.26650/JARHS2020-668235



TIMELINE


Submitted31.12.2019
Accepted10.02.2020
Published Online26.02.2020

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