Research Article


DOI :10.26650/IUITFD.2021.896230   IUP :10.26650/IUITFD.2021.896230    Full Text (PDF)

THE RELATIONSHIP BETWEEN THE EXPRESSION LEVELS OF TISSUE INHIBITOR OF METALLOPROTEINASES-3 (TIMP3) AND SEVERITY OF ATHEROSCLEROSIS

Gizem ÇelebiFiliz Güçlü GeyikEmine Dilek YılmazbayhanSadiye Deniz ÖzsoyCenk Eray YıldızMustafa YıldızDoğaç ÖksenMehmet CavlakAyşe Evrim Bayrak

Objective: TIMP3 is one of tissue inhibitors of metalloproteinases (TIMPs), which binds to the components of the extracellular matrix, and has crucial roles in atherosclerogenesis and adipose tissue differentiation. In this study, it was aimed to determine the effects of TIMP3 gene expression levels on severity of atherosclerosis in different tissues. Material and Methods: The first group of the study (evaluated for coronary artery disease) were cases classified as high and low plaque scores according to the degree and location of atherosclerotic lesions. In the second group (post-mortem cases) were male cadavers who died due to coronary heart disease (CHD, n=26) and non-cardiac trauma (T-nonP, n=4). The TIMP3 expression levels were examined in leukocyte and peri-coronary epicardial adipose tissues (EAT) samples (n=69 and n=34, respectively) of the first group and EAT and coronary artery samples (n=12 and n=30, respectively) of the second group using quantitative RT-PCR. In addition, the protein expressions of TIMP3 were analysed on artery sections by immunofluorestaining. Results: In the post-mortem study group, the TIMP3 expression levels were found to increase in no plaque segments of arteries of cases with CHD (CHD-nonP) compared to advanced atherosclerotic arteries (CHD-P) and normal arteries of T-nonP cases (p=0.01 and p=0.05, respectively). The TIMP3 expressions in EATs and leukocytes were not statistically significant between the study groups. In addition, TIMP3 protein was detected in normal arteries, peri-coronary EATs and mostly in macrophages-rich areas in advanced atherosclerotic arteries. Conclusion: TIMP3 expression levels increase in normal coronary arterial segments of cases with CHD, which depict a protective role of TIMP3 in development of atherosclerosis.

DOI :10.26650/IUITFD.2021.896230   IUP :10.26650/IUITFD.2021.896230    Full Text (PDF)

METALLOPROTEİNAZ-3 DOKU İNHİBİTÖRÜNÜN (TIMP3) İFADE DÜZEYLERİ İLE ATEROSKLEROZUN ŞİDDETİ ARASINDAKİ İLİŞKİ

Gizem ÇelebiFiliz Güçlü GeyikEmine Dilek YılmazbayhanSadiye Deniz ÖzsoyCenk Eray YıldızMustafa YıldızDoğaç ÖksenMehmet CavlakAyşe Evrim Bayrak

Amaç: Hücre dışı matriksin bileşenlerine bağlanan TIMP3, metaloproteinazların doku inhibitörlerinden (TIMP’ler) biridir ve ateroskleroz gelişimi ile yağ dokusu farklılaşmasında rol oynamaktadır. Bu çalışmada, farklı dokulardaki TIMP3 gen ifade düzeylerinin ateroskleroz şiddeti üzerine olan etkisinin belirlenmesi amaçlandı. Gereç ve Yöntemler: Çalışmanın ilk grubu (koroner arter hastalığı için değerlendirilen), aterosklerotik lezyonların derecesine ve yerine göre yüksek ve düşük plak skoru olarak sınıflandırılan vakalardı. İkinci grup (post-mortem vakalar), koroner kalp hastalığı (CHD, n=26) ve kardiyak olmayan travma (T-nonP, n=4) nedeniyle ölen erkek kadavralardı. Birinci grubun lökosit ve peri-koroner epikardiyal yağ doku (EYD) örnekleri (sırasıyla, n=69 ve n=34) ile ikinci grubun EYD ve koroner arter örneklerinde (sırasıyla, n=12 ve n=30) TIPM3 ifade düzeyleri kantitatif RT-PCR ile incelendi. Ek olarak, TIMP3 protein lokalizasyonları, immunfluoresans tekniği ile belirlendi. Bulgular: Post-mortem çalışma grubunda, TIMP3 ifade düzeylerinin CHD’li vakaların plaksız arter segmentlerinde (CHD-nonP), ileri aterosklerotik arterlere (CHD-P) ve T-nonP vakaların normal arterlere kıyasla arttığı bulundu (sırasıyla, p=0.01 ve p=0.05). Lökosit ve EYD’lerdeki TIMP3 gen ifadeleri, çalışma grupları arasında istatistiksel olarak anlamlı fark göstermedi. Ek olarak, TIMP3 proteini normal arter, peri-koroner EYD ve ileri aterosklerotik arterde makrofajların yoğun olduğu alanlarda tespit edildi. Sonuç: CHD’li vakaların normal koroner arteriyel segmentlerinde TIMP3 ifadesindeki artışın ateroskleroz gelişimine karşı koruyucu bir etkisi olabileceğini göstermektedir.


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APA

Çelebi, G., Güçlü Geyik, F., Yılmazbayhan, E., Özsoy, S.D., Yıldız, C.E., Yıldız, M., Öksen, D., Cavlak, M., & Bayrak, A.E. (2021). THE RELATIONSHIP BETWEEN THE EXPRESSION LEVELS OF TISSUE INHIBITOR OF METALLOPROTEINASES-3 (TIMP3) AND SEVERITY OF ATHEROSCLEROSIS. Journal of Istanbul Faculty of Medicine, 84(4), 472-481. https://doi.org/10.26650/IUITFD.2021.896230


AMA

Çelebi G, Güçlü Geyik F, Yılmazbayhan E, Özsoy S D, Yıldız C E, Yıldız M, Öksen D, Cavlak M, Bayrak A E. THE RELATIONSHIP BETWEEN THE EXPRESSION LEVELS OF TISSUE INHIBITOR OF METALLOPROTEINASES-3 (TIMP3) AND SEVERITY OF ATHEROSCLEROSIS. Journal of Istanbul Faculty of Medicine. 2021;84(4):472-481. https://doi.org/10.26650/IUITFD.2021.896230


ABNT

Çelebi, G.; Güçlü Geyik, F.; Yılmazbayhan, E.; Özsoy, S.D.; Yıldız, C.E.; Yıldız, M.; Öksen, D.; Cavlak, M.; Bayrak, A.E. THE RELATIONSHIP BETWEEN THE EXPRESSION LEVELS OF TISSUE INHIBITOR OF METALLOPROTEINASES-3 (TIMP3) AND SEVERITY OF ATHEROSCLEROSIS. Journal of Istanbul Faculty of Medicine, [Publisher Location], v. 84, n. 4, p. 472-481, 2021.


Chicago: Author-Date Style

Çelebi, Gizem, and Filiz Güçlü Geyik and Emine Dilek Yılmazbayhan and Sadiye Deniz Özsoy and Cenk Eray Yıldız and Mustafa Yıldız and Doğaç Öksen and Mehmet Cavlak and Ayşe Evrim Bayrak. 2021. “THE RELATIONSHIP BETWEEN THE EXPRESSION LEVELS OF TISSUE INHIBITOR OF METALLOPROTEINASES-3 (TIMP3) AND SEVERITY OF ATHEROSCLEROSIS.” Journal of Istanbul Faculty of Medicine 84, no. 4: 472-481. https://doi.org/10.26650/IUITFD.2021.896230


Chicago: Humanities Style

Çelebi, Gizem, and Filiz Güçlü Geyik and Emine Dilek Yılmazbayhan and Sadiye Deniz Özsoy and Cenk Eray Yıldız and Mustafa Yıldız and Doğaç Öksen and Mehmet Cavlak and Ayşe Evrim Bayrak. THE RELATIONSHIP BETWEEN THE EXPRESSION LEVELS OF TISSUE INHIBITOR OF METALLOPROTEINASES-3 (TIMP3) AND SEVERITY OF ATHEROSCLEROSIS.” Journal of Istanbul Faculty of Medicine 84, no. 4 (Oct. 2021): 472-481. https://doi.org/10.26650/IUITFD.2021.896230


Harvard: Australian Style

Çelebi, G & Güçlü Geyik, F & Yılmazbayhan, E & Özsoy, SD & Yıldız, CE & Yıldız, M & Öksen, D & Cavlak, M & Bayrak, AE 2021, 'THE RELATIONSHIP BETWEEN THE EXPRESSION LEVELS OF TISSUE INHIBITOR OF METALLOPROTEINASES-3 (TIMP3) AND SEVERITY OF ATHEROSCLEROSIS', Journal of Istanbul Faculty of Medicine, vol. 84, no. 4, pp. 472-481, viewed 20 Oct. 2021, https://doi.org/10.26650/IUITFD.2021.896230


Harvard: Author-Date Style

Çelebi, G. and Güçlü Geyik, F. and Yılmazbayhan, E. and Özsoy, S.D. and Yıldız, C.E. and Yıldız, M. and Öksen, D. and Cavlak, M. and Bayrak, A.E. (2021) ‘THE RELATIONSHIP BETWEEN THE EXPRESSION LEVELS OF TISSUE INHIBITOR OF METALLOPROTEINASES-3 (TIMP3) AND SEVERITY OF ATHEROSCLEROSIS’, Journal of Istanbul Faculty of Medicine, 84(4), pp. 472-481. https://doi.org/10.26650/IUITFD.2021.896230 (20 Oct. 2021).


MLA

Çelebi, Gizem, and Filiz Güçlü Geyik and Emine Dilek Yılmazbayhan and Sadiye Deniz Özsoy and Cenk Eray Yıldız and Mustafa Yıldız and Doğaç Öksen and Mehmet Cavlak and Ayşe Evrim Bayrak. THE RELATIONSHIP BETWEEN THE EXPRESSION LEVELS OF TISSUE INHIBITOR OF METALLOPROTEINASES-3 (TIMP3) AND SEVERITY OF ATHEROSCLEROSIS.” Journal of Istanbul Faculty of Medicine, vol. 84, no. 4, 2021, pp. 472-481. [Database Container], https://doi.org/10.26650/IUITFD.2021.896230


Vancouver

Çelebi G, Güçlü Geyik F, Yılmazbayhan E, Özsoy SD, Yıldız CE, Yıldız M, Öksen D, Cavlak M, Bayrak AE. THE RELATIONSHIP BETWEEN THE EXPRESSION LEVELS OF TISSUE INHIBITOR OF METALLOPROTEINASES-3 (TIMP3) AND SEVERITY OF ATHEROSCLEROSIS. Journal of Istanbul Faculty of Medicine [Internet]. 20 Oct. 2021 [cited 20 Oct. 2021];84(4):472-481. Available from: https://doi.org/10.26650/IUITFD.2021.896230 doi: 10.26650/IUITFD.2021.896230


ISNAD

Çelebi, Gizem - Güçlü Geyik, Filiz - Yılmazbayhan, Emine Dilek - Özsoy, SadiyeDeniz - Yıldız, CenkEray - Yıldız, Mustafa - Öksen, Doğaç - Cavlak, Mehmet - Bayrak, AyşeEvrim. THE RELATIONSHIP BETWEEN THE EXPRESSION LEVELS OF TISSUE INHIBITOR OF METALLOPROTEINASES-3 (TIMP3) AND SEVERITY OF ATHEROSCLEROSIS”. Journal of Istanbul Faculty of Medicine 84/4 (Oct. 2021): 472-481. https://doi.org/10.26650/IUITFD.2021.896230



TIMELINE


Submitted13.03.2021
Accepted03.05.2021
Published Online13.09.2021

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