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DOI :10.26650/EurJBiol.2022.1078448   IUP :10.26650/EurJBiol.2022.1078448    Tam Metin (PDF)

Identification of Fusarium graminearum and Fusarium culmorum Isolates via Conventional and Molecular Methods

Beliz YüksektepeÖzlem SeferGülin İnci VarolTuğba TekerMehmet ArslanBüşra Nur ÇetinFigen MertEmre YörükGülruh Albayrak

Objective: Fusarium spp. cause Fusarium head blight (FHB) and crown rot (CR) diseases. They also have harmful effects on animal and human health through their mycotoxins. Within the scope of this study, F. graminearum and F. culmorum isolates were purified from wheat ears and stalks contaminated with phytopathogens, which had been collected from various regions of Turkey, were identified and characterized by conventional and molecular methods. Materials and Methods: Sixty-eight Fusarium samples were isolated by single spore analysis and classified according to their macroconidia shape and size. Morphologically characterized samples were verified by amplification of SCAR markers. Their mating types (MAT) and chemotypes were also determined through polymerase chain reaction (PCR). Results: Thirty-eight F. graminearum and 30 F. culmorum isolates were identified via amplification of UBC85 and OPT18 SCAR markers, respectively. All isolates were determined as trichothecene producers by amplification of the tri5 gene. All F. graminarum isolates carry both MAT-1 and MAT-2 loci, whereas 7 of F. culmorum isolates were also determined as MAT-1 and 23 of them as MAT-2 mating types. Deoxynivalenol production capacity of all isolates was identified by tri13 amplification for chemotype determination. Conclusion: Routine monitoring of phytopathogens and their mycotoxin levels is a requirement since their annual levels may vary depending on environmental factors. This work provides knowledge about the distribution of Fusarium spp. leading to FHB and CR in different regions of Turkey between 2010 and 2020. Also, their chemotypes were demonstrated. Our studies will contribute to disease profiling and it is the first step in disease management.


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DIŞA AKTAR



APA

Yüksektepe, B., Sefer, Ö., Varol, G.İ., Teker, T., Arslan, M., Çetin, B.N., Mert, F., Yörük, E., & Albayrak, G. (2022). Identification of Fusarium graminearum and Fusarium culmorum Isolates via Conventional and Molecular Methods. European Journal of Biology, 81(1), 107-116. https://doi.org/10.26650/EurJBiol.2022.1078448


AMA

Yüksektepe B, Sefer Ö, Varol G İ, Teker T, Arslan M, Çetin B N, Mert F, Yörük E, Albayrak G. Identification of Fusarium graminearum and Fusarium culmorum Isolates via Conventional and Molecular Methods. European Journal of Biology. 2022;81(1):107-116. https://doi.org/10.26650/EurJBiol.2022.1078448


ABNT

Yüksektepe, B.; Sefer, Ö.; Varol, G.İ.; Teker, T.; Arslan, M.; Çetin, B.N.; Mert, F.; Yörük, E.; Albayrak, G. Identification of Fusarium graminearum and Fusarium culmorum Isolates via Conventional and Molecular Methods. European Journal of Biology, [Publisher Location], v. 81, n. 1, p. 107-116, 2022.


Chicago: Author-Date Style

Yüksektepe, Beliz, and Özlem Sefer and Gülin İnci Varol and Tuğba Teker and Mehmet Arslan and Büşra Nur Çetin and Figen Mert and Emre Yörük and Gülruh Albayrak. 2022. “Identification of Fusarium graminearum and Fusarium culmorum Isolates via Conventional and Molecular Methods.” European Journal of Biology 81, no. 1: 107-116. https://doi.org/10.26650/EurJBiol.2022.1078448


Chicago: Humanities Style

Yüksektepe, Beliz, and Özlem Sefer and Gülin İnci Varol and Tuğba Teker and Mehmet Arslan and Büşra Nur Çetin and Figen Mert and Emre Yörük and Gülruh Albayrak. Identification of Fusarium graminearum and Fusarium culmorum Isolates via Conventional and Molecular Methods.” European Journal of Biology 81, no. 1 (Dec. 2022): 107-116. https://doi.org/10.26650/EurJBiol.2022.1078448


Harvard: Australian Style

Yüksektepe, B & Sefer, Ö & Varol, Gİ & Teker, T & Arslan, M & Çetin, BN & Mert, F & Yörük, E & Albayrak, G 2022, 'Identification of Fusarium graminearum and Fusarium culmorum Isolates via Conventional and Molecular Methods', European Journal of Biology, vol. 81, no. 1, pp. 107-116, viewed 2 Dec. 2022, https://doi.org/10.26650/EurJBiol.2022.1078448


Harvard: Author-Date Style

Yüksektepe, B. and Sefer, Ö. and Varol, G.İ. and Teker, T. and Arslan, M. and Çetin, B.N. and Mert, F. and Yörük, E. and Albayrak, G. (2022) ‘Identification of Fusarium graminearum and Fusarium culmorum Isolates via Conventional and Molecular Methods’, European Journal of Biology, 81(1), pp. 107-116. https://doi.org/10.26650/EurJBiol.2022.1078448 (2 Dec. 2022).


MLA

Yüksektepe, Beliz, and Özlem Sefer and Gülin İnci Varol and Tuğba Teker and Mehmet Arslan and Büşra Nur Çetin and Figen Mert and Emre Yörük and Gülruh Albayrak. Identification of Fusarium graminearum and Fusarium culmorum Isolates via Conventional and Molecular Methods.” European Journal of Biology, vol. 81, no. 1, 2022, pp. 107-116. [Database Container], https://doi.org/10.26650/EurJBiol.2022.1078448


Vancouver

Yüksektepe B, Sefer Ö, Varol Gİ, Teker T, Arslan M, Çetin BN, Mert F, Yörük E, Albayrak G. Identification of Fusarium graminearum and Fusarium culmorum Isolates via Conventional and Molecular Methods. European Journal of Biology [Internet]. 2 Dec. 2022 [cited 2 Dec. 2022];81(1):107-116. Available from: https://doi.org/10.26650/EurJBiol.2022.1078448 doi: 10.26650/EurJBiol.2022.1078448


ISNAD

Yüksektepe, Beliz - Sefer, Özlem - Varol, Gülinİnci - Teker, Tuğba - Arslan, Mehmet - Çetin, BüşraNur - Mert, Figen - Yörük, Emre - Albayrak, Gülruh. Identification of Fusarium graminearum and Fusarium culmorum Isolates via Conventional and Molecular Methods”. European Journal of Biology 81/1 (Dec. 2022): 107-116. https://doi.org/10.26650/EurJBiol.2022.1078448



ZAMAN ÇİZELGESİ


Gönderim24.02.2022
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