The Effect of Rectal Ozone Use on Bacterial  Translocation and Oxidative Stress in Experimental  Colitis Model

Bostancı Meriç; Avcı Onur; Taş Ayça; Koç Tülay; Gürsoy Sinan; Siliğ Yavuz

doi:https://dx.doi.org/10.26650/experimed.1102002

Araştırma Makalesi

DOI :10.26650/experimed.1102002 IUP :10.26650/experimed.1102002 Tam Metin (PDF)

The Effect of Rectal Ozone Use on Bacterial Translocation and Oxidative Stress in Experimental Colitis Model

Meriç Emre Bostancı, Onur Avcı, Ayça Taş, Tülay Koç, Sinan Gürsoy, Yavuz Siliğ

Objective: The aim of this study was to examine the effect of rectal ozone on the histopathological healing of the colonic mucosa, tissue oxidative stress, and bacterial translocation in the experimental colitis model. Materials and Methods: Three groups of rats were randomly formed [Group 1: Sham group, group 2: Control group, group 3: Ozone treatment group]. Microscopic and macroscopic scoring were done histopathologically in all groups. Glutathione-s-transferase (GST), superoxide dismutase (SOD), glutathione (GSH), and malondialdehyde (MDA) levels were measured in the colon and liver tissue. Blood cultures were taken for the detection of bacterial translocation. Results: Microscopic damage scores were found as 0.0 (0.0-2.0) in the sham group, 3.0 (3.0-3.0) in the control group, 2.0 (0.0-2.0) in the ozone treatment group (p=0.001). Macroscopic damage scores were found as 0.0 (0.0-1.0) in the sham group, 3.0 (0.0-4.0) in the control group, 0.0 (0.0-1.0) in the ozone treatment group; the scores of ozone treatment and sham groups were found to be statistically different (p=0.004). Compared to the control group, the bacterial translocation in the liver, mesenteric lymph node, portal vein, spleen, and systemic blood was fewer in the ozone treatment group. Statistically significant differences were also observed between the groups’ SOD and GST levels in colon tissue and MDA, SOD, and GST levels in liver tissue. Regarding MDA values in the liver tissue of the groups, it was 1.95±0.43 in Group I, 3.63±0.81 in Group II, and 1.19±0.72 U/mg in Group III (p=0.017). When the liver tissue SOD levels of the groups were examined, it was 8.21±0.76 U/mg in Group I, 4.57±0.58 U/mg in Group II, and 8.62±1.13 U/mg in Group III (p=0.029). When GST values in liver tissue belonging to the groups were examined, 0.35±0.16 in Group 1, 0.23±0.03 in Group II, 0.49±0.13 U/mg in Group III (p=0.036). Conclusion: Rectal ozone application plays a role in increasing the organism’s antioxidant activity and has an effective role in increasing the enzyme activities of antioxidant defenses. In addition, rectal ozone application has a positive effect on wound healing at a histopathological level and reduces bacterial translocation in various tissues.

Anahtar Kelimeler: Experimental colitis, rectal ozone, oxidative stress

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DIŞA AKTAR

APA

Bostancı, M.E., Avcı, O., Taş, A., Koç, T., Gürsoy, S., & Siliğ, Y. (2022). The Effect of Rectal Ozone Use on Bacterial Translocation and Oxidative Stress in Experimental Colitis Model. Experimed, 12(2), 66-73. https://doi.org/10.26650/experimed.1102002

AMA

Bostancı M E, Avcı O, Taş A, Koç T, Gürsoy S, Siliğ Y. The Effect of Rectal Ozone Use on Bacterial Translocation and Oxidative Stress in Experimental Colitis Model. Experimed. 2022;12(2):66-73. https://doi.org/10.26650/experimed.1102002

ABNT

Bostancı, M.E.; Avcı, O.; Taş, A.; Koç, T.; Gürsoy, S.; Siliğ, Y. The Effect of Rectal Ozone Use on Bacterial Translocation and Oxidative Stress in Experimental Colitis Model. Experimed, [Publisher Location], v. 12, n. 2, p. 66-73, 2022.

Chicago: Author-Date Style

Bostancı, Meriç Emre, and Onur Avcı and Ayça Taş and Tülay Koç and Sinan Gürsoy and Yavuz Siliğ. 2022. “The Effect of Rectal Ozone Use on Bacterial Translocation and Oxidative Stress in Experimental Colitis Model.” Experimed 12, no. 2: 66-73. https://doi.org/10.26650/experimed.1102002

Chicago: Humanities Style

Bostancı, Meriç Emre, and Onur Avcı and Ayça Taş and Tülay Koç and Sinan Gürsoy and Yavuz Siliğ. “The Effect of Rectal Ozone Use on Bacterial Translocation and Oxidative Stress in Experimental Colitis Model.” Experimed 12, no. 2 (Apr. 2023): 66-73. https://doi.org/10.26650/experimed.1102002

Harvard: Australian Style

Bostancı, ME & Avcı, O & Taş, A & Koç, T & Gürsoy, S & Siliğ, Y 2022, 'The Effect of Rectal Ozone Use on Bacterial Translocation and Oxidative Stress in Experimental Colitis Model', Experimed, vol. 12, no. 2, pp. 66-73, viewed 1 Apr. 2023, https://doi.org/10.26650/experimed.1102002

Harvard: Author-Date Style

Bostancı, M.E. and Avcı, O. and Taş, A. and Koç, T. and Gürsoy, S. and Siliğ, Y. (2022) ‘The Effect of Rectal Ozone Use on Bacterial Translocation and Oxidative Stress in Experimental Colitis Model’, Experimed, 12(2), pp. 66-73. https://doi.org/10.26650/experimed.1102002 (1 Apr. 2023).

MLA

Bostancı, Meriç Emre, and Onur Avcı and Ayça Taş and Tülay Koç and Sinan Gürsoy and Yavuz Siliğ. “The Effect of Rectal Ozone Use on Bacterial Translocation and Oxidative Stress in Experimental Colitis Model.” Experimed, vol. 12, no. 2, 2022, pp. 66-73. [Database Container], https://doi.org/10.26650/experimed.1102002

Vancouver

Bostancı ME, Avcı O, Taş A, Koç T, Gürsoy S, Siliğ Y. The Effect of Rectal Ozone Use on Bacterial Translocation and Oxidative Stress in Experimental Colitis Model. Experimed [Internet]. 1 Apr. 2023 [cited 1 Apr. 2023];12(2):66-73. Available from: https://doi.org/10.26650/experimed.1102002 doi: 10.26650/experimed.1102002

ISNAD

Bostancı, MeriçEmre - Avcı, Onur - Taş, Ayça - Koç, Tülay - Gürsoy, Sinan - Siliğ, Yavuz. “The Effect of Rectal Ozone Use on Bacterial Translocation and Oxidative Stress in Experimental Colitis Model”. Experimed 12/2 (Apr. 2023): 66-73. https://doi.org/10.26650/experimed.1102002

Cilt 12, Sayı 22022, S. 66-73

ZAMAN ÇİZELGESİ

Gönderim	15.04.2022
Kabul	05.07.2022
Çevrimiçi Yayınlanma	11.08.2022

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