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DOI :10.26650/IstanbulJPharm.2019.19020   IUP :10.26650/IstanbulJPharm.2019.19020    Tam Metin (PDF)

Bismuth oxide nanoparticles induced oxidative stress-related inflammation in SH-SY5Y cell line

Ezgi ÖztaşMahmoud AbudayyakBeyza AykanatZübeyde CanEnes BaramGül Özhan

Bismuth (III) oxide nanoparticles’ (Bi2 O3 -NPs) unique physicochemical properties have attracted attention in biological, industrial, technological and medical fields. Concurrently, increasing numbers of studies revealing their potential toxic effects and possible toxicity mechanisms are ongoing. In this study, we assessed the toxic potentials of Bi2 O3 -NPs in human SH-SY5Y neuroblastoma cell line. After Bi2 O3 -NPs characterization using TEM, the cytotoxic potentials were evaluated by MTT and LDH assays. The induction of reactive oxygen species production was evaluated by H2 DCFDA. In order to evaluate the oxidative damages, the changes in antioxidant catalase and superoxide dismutase and glutathione levels were determined. The cellular death pathway and the role of immune response were studied by measuring the mRNA expression levels of related genes. Our results showed that Bi2 O3 -NPs decreased the cell viability through disruption on mitochondrial activity (IC50:77.57 µg/mL) and membrane integrity (LDH%50:16.97 µg/mL). At 50 µg/mL Bi2 O3 -NPs, the production of reactive oxygen species (ROS) was induced significantly as well as the catalase and superoxide dismutase levels. In immune response, the mRNA expression levels of interleukin (IL)-6 increased more than 1.5-fold in all doses; whereas, TNF-α, NF-ĸB and MAPK8 expressions remained unchanged. Consequently, Bi2 O3 -NPs induced oxidative stress-related inflammation via activation of proinflammatory cytokine, IL-6. 


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APA

Öztaş, E., Abudayyak, M., Aykanat, B., Can, Z., Baram, E., & Özhan, G. (2019). Bismuth oxide nanoparticles induced oxidative stress-related inflammation in SH-SY5Y cell line. İstanbul Journal of Pharmacy, 49(3), 173-179. https://doi.org/10.26650/IstanbulJPharm.2019.19020


AMA

Öztaş E, Abudayyak M, Aykanat B, Can Z, Baram E, Özhan G. Bismuth oxide nanoparticles induced oxidative stress-related inflammation in SH-SY5Y cell line. İstanbul Journal of Pharmacy. 2019;49(3):173-179. https://doi.org/10.26650/IstanbulJPharm.2019.19020


ABNT

Öztaş, E.; Abudayyak, M.; Aykanat, B.; Can, Z.; Baram, E.; Özhan, G. Bismuth oxide nanoparticles induced oxidative stress-related inflammation in SH-SY5Y cell line. İstanbul Journal of Pharmacy, [Publisher Location], v. 49, n. 3, p. 173-179, 2019.


Chicago: Author-Date Style

Öztaş, Ezgi, and Mahmoud Abudayyak and Beyza Aykanat and Zübeyde Can and Enes Baram and Gül Özhan. 2019. “Bismuth oxide nanoparticles induced oxidative stress-related inflammation in SH-SY5Y cell line.” İstanbul Journal of Pharmacy 49, no. 3: 173-179. https://doi.org/10.26650/IstanbulJPharm.2019.19020


Chicago: Humanities Style

Öztaş, Ezgi, and Mahmoud Abudayyak and Beyza Aykanat and Zübeyde Can and Enes Baram and Gül Özhan. Bismuth oxide nanoparticles induced oxidative stress-related inflammation in SH-SY5Y cell line.” İstanbul Journal of Pharmacy 49, no. 3 (Feb. 2023): 173-179. https://doi.org/10.26650/IstanbulJPharm.2019.19020


Harvard: Australian Style

Öztaş, E & Abudayyak, M & Aykanat, B & Can, Z & Baram, E & Özhan, G 2019, 'Bismuth oxide nanoparticles induced oxidative stress-related inflammation in SH-SY5Y cell line', İstanbul Journal of Pharmacy, vol. 49, no. 3, pp. 173-179, viewed 4 Feb. 2023, https://doi.org/10.26650/IstanbulJPharm.2019.19020


Harvard: Author-Date Style

Öztaş, E. and Abudayyak, M. and Aykanat, B. and Can, Z. and Baram, E. and Özhan, G. (2019) ‘Bismuth oxide nanoparticles induced oxidative stress-related inflammation in SH-SY5Y cell line’, İstanbul Journal of Pharmacy, 49(3), pp. 173-179. https://doi.org/10.26650/IstanbulJPharm.2019.19020 (4 Feb. 2023).


MLA

Öztaş, Ezgi, and Mahmoud Abudayyak and Beyza Aykanat and Zübeyde Can and Enes Baram and Gül Özhan. Bismuth oxide nanoparticles induced oxidative stress-related inflammation in SH-SY5Y cell line.” İstanbul Journal of Pharmacy, vol. 49, no. 3, 2019, pp. 173-179. [Database Container], https://doi.org/10.26650/IstanbulJPharm.2019.19020


Vancouver

Öztaş E, Abudayyak M, Aykanat B, Can Z, Baram E, Özhan G. Bismuth oxide nanoparticles induced oxidative stress-related inflammation in SH-SY5Y cell line. İstanbul Journal of Pharmacy [Internet]. 4 Feb. 2023 [cited 4 Feb. 2023];49(3):173-179. Available from: https://doi.org/10.26650/IstanbulJPharm.2019.19020 doi: 10.26650/IstanbulJPharm.2019.19020


ISNAD

Öztaş, Ezgi - Abudayyak, Mahmoud - Aykanat, Beyza - Can, Zübeyde - Baram, Enes - Özhan, Gül. Bismuth oxide nanoparticles induced oxidative stress-related inflammation in SH-SY5Y cell line”. İstanbul Journal of Pharmacy 49/3 (Feb. 2023): 173-179. https://doi.org/10.26650/IstanbulJPharm.2019.19020



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Kabul20.09.2019

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