İstanbul Journal of Pharmacy

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DOI :10.26650/IstanbulJPharm.2022.1094112   IUP :10.26650/IstanbulJPharm.2022.1094112    Tam Metin (PDF)

Effect of flurbiprofen derivative (SGK597) on cell proliferation and apoptosis of breast cancer cell lines

İrem Atlıhan, Sevgi Koçyiğit Sevinç, Oya Orun, Özgür Yılmaz, Şükriye Güniz Küçükgüzel, Pınar Mega Tiber

Background and Aims: The incidence of breast cancer is increasing day by day, especially in women. The search for new drugs against breast cancer is the focus of attention in research. Breast cancer and prostate cancer have remarkable biological similarities. Therefore, the 4-(4-chlorophenyl)-3-(1-(2-fluoro-[1,1'-biphenyl]-4-yl)ethyl)-5-((4-fluorobenzyl)thio)-4H-1,2,4-triazole (SGK597) compound that is suppressing cell proliferation in prostate cancer, was studied in MCF-7 breast cancer and MCF-10A mammary epithelial cell lines. Methods: The WST-8 method was used to determine cell viability and cytotoxicity of SGK597 in MCF-7 and MCF10-A cell lines. The JC-1 test was applied to determine changes in mitochondrial membrane potential. The protein expression levels of Bax, Bcl-2, and c-PARP associated with apoptosis were determined using Western blot analysis. Results: After 24 and 48 hours of incubation of SGK597, the IC50 values were 28.74 μM and 17.28 μM for MCF-7; 65.9 μM and 50.5 μM for MCF-10A, respectively. Mitochondrial membrane potential showed a tendency toward depolarization in MCF-7 cells as a result of increasing concentration of SGK597, while the same tendency was not seen for MCF-10A. As a result of western blot experiments, no increase in the Bax/Bcl-2 ratio and c-PARP expression level was observed, indicating no apoptosis. Conclusion: It was observed that the compound SGK597 suppressed MCF-7 cell proliferation. These results indicate that SGK597 may be a candidate compound for use as an anticancer agent.

Anahtar Kelimeler: Apoptosis, breast cancer, flurbiprofen, thioether, triazole

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Referanslar

  • Ali, R., Mirza, Z., Ashraf, G. M., Kamal, M. A., Ansari, S. A., Daman-houri, G. A., ... Sheikh, I. A. (2012). New anticancer agents: recent developments in tumor therapy. Anticancer Research, 32(7), 2999-3005. google scholar
  • Aydın, S., Kaushik-Basu, N., Arora, P., Basu, A., Nichols, D. B., Talele, T. T., . Küçükgüzel, Ş. G. (2013). Microwave assisted synthesis of some novel Flurbiprofen hydrazidehydrazones as anti-HCV NS5B and anticancer agents. Marmara Pharmaceutical Journal, 17, 2634. google scholar
  • Bakır, E., Çal, T., Aydın Dilsiz, S., Canpınar, H., Eken, A., & Ündeğer Bucurgat, Ü. (2021). Assessment of the cytotoxic, genotoxic, and apoptotic potential of flurbiprofen in HeLa and HepG2 cell lines. Journal of biochemical and molecular toxicology, 35(6), 1-11. https://doi.org/10.1002/jbt.22770 google scholar
  • Becker S. (2015). A historic and scientific review of breast cancer: The next global healthcare challenge. International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics, 131 Suppl 1, S36-S39. https:// doi.org/10.1016/j.ijgo.2015.03.015 google scholar
  • Birgül, K., Yıldırım, Y., Karasulu, H. Y., Karasulu, E., Uba, A. I., Yelekçi, K., . Küçükgüzel, Ş. G. (2020). Synthesis, molecular modeling, in vivo study and anticancer activity against prostate cancer of (+) (S)-naproxen derivatives. European journal of medicinal chemistry, 208, 112841. https://doi.org/10.1016/j.ejmech.2020.112841 google scholar
  • Bradke, T. M., Hall, C., Carper, S. W., & Plopper, G. E. (2008). Phenyl-boronic acid selectively inhibits human prostate and breast cancer cell migration and decreases viability. Cell adhesion & migration, 2(3), 153-160. https://doi.org/10.4161/cam.2.3.6484 google scholar
  • Çıkla, P., Tatar, E., Küçükgüzel, İ., Şahin, F., Yurdakul, D., Basu, A., . Küçükgüzel, Ş. G. (2013). Synthesis and characterization of flurbiprofen hydrazide derivatives as potential anti-HCV, anticancer and antimicrobial agents. Medicinal Chemistry Research, 22(12), 5685-5999. https://doi.org/10.1007/s00044-013-0550-3 google scholar
  • Çıkla-Süzgün, P., & Küçükgüzel, Ş. G. (2021). Recent Progress on Apoptotic Activity of Triazoles. Current drug targets, 22(16), 18441900. https://doi.org/10.2174/1389450122666210208181128 google scholar
  • Çoruh, I., Çevik, Ö., Yelekçi, K., Djikic, T., & Küçükgüzel, Ş. G. (2018). Synthesis, anticancer activity, and molecular modeling of etodol-ac-thioether derivatives as potent methionine aminopeptidase (type II) inhibitors. Archiv der Pharmazie, 351(3-4), e1700195. https://doi.org/10.1002/ardp.201700195 google scholar
  • Fröhlich, T., Mai, C., Bogautdinov, R. P., Morozkina, S. N., Shavva, A. G., Friedrich, O., . Tsogoeva, S. B. (2020). Synthesis of Tamox-ifen-Artemisinin and Estrogen-Artemisinin Hybrids Highly Potent Against Breast and Prostate Cancer. ChemMedChem, 15(15), 1473-1479. https://doi.org/10.1002/cmdc.202000174 google scholar
  • Han, M. İ., & Küçükgüzel, Ş. G. (2022). Thioethers: An Overview. Current drug targets, 23(2), 170-219. https://doi.org/10.2174/138 9450122666210614121237 google scholar
  • Imai, M., Yokoe, H., Tsubuki, M., & Takahashi, N. (2019). Growth Inhibition of Human Breast and Prostate Cancer Cells by Cinnamic Acid Derivatives and Their Mechanism of Action. Biological & pharmaceutical bulletin, 42(7), 1134-1139. https://doi. org/10.1248/bpb.b18-01002 google scholar
  • King, Jr, J., & Khalili, K. (2001). Inhibition of human brain tumor cell growth by the anti-inflammatory drug, flurbiprofen. Oncogene, 20(47), 6864-6870. https://doi.org/10.1038/sj.onc.1204907 google scholar
  • Kujukguzel, J. G., & Qkla-Suzgun, P (2015). Recent advances bioactive 1,2,4-triazole-3-thiones. European journal of medicinal chemistry, 97, 830-870. https://doi.org/10.1016/j.ej-mech.2014.11.033 google scholar
  • Liao, S., Umekita, Y., Guo, J., Kokontis, J. M., & Hiipakka, R. A. (1995). Growth inhibition and regression of human prostate and breast tumors in athymic mice by tea epigallocatechin gallate. Cancer letters, 96(2), 239-243. https://doi.org/10.1016/0304-3835(95)03948-v Ly, J. D., Grubb, D. R., & Lawen, A. (2003). The mitochondrial membrane potential (deltapsi(m)) in apoptosis; an update. Apoptosis: an international journal on programmed cell death, 8(2), 115-128. https://doi.org/10.1023/a:1022945107762 google scholar
  • McCormick, D. L., & Moon, R. C. (1983). Inhibition of mammary carcinogenesis by flurbiprofen, a non-steroidal antiinflammatory agent. British Journal of Cancer 48(6), 859-861. https://doi. org/10.1038/bjc.1983.278 google scholar
  • Meegan, M. J., & O’Boyle, N. M. (2019). Special Issue “Anticancer Drugs”. Pharmaceuticals (Basel, Switzerland), 12(3), 134. https://doi. org/10.3390/ph12030134 google scholar
  • Mega Tiber, P., Kocyigit Sevinc, S., Kilinc, O., & Orun, O. (2019). Biological effects of whole Z. officinale extract on chronic myeloid leukemia cell line K562. Gene, 692, 217-222. https://doi. org/10.1016/j.gene.2019.01.015 google scholar
  • Powles, T. J., Alexander, P., & Millar, J. L. (1978). Enhancement of anti-cancer activity of cytotoxic chemotherapy with protection of normal tissues by inhibition of P.G. synthesis. Biochemical Pharmacology, 27(9), 1389-1392. https://doi.org/10.1016/0006-2952(78)90127-2 google scholar
  • Ren, Z.-J., Cao, D.-H., Zhang, Q., Ren, P.-W, Liu, L-R., Wei, Q..... google scholar
  • Dong, Q. (2019). First-degree family history of breast cancer is associated with prostate cancer risk: a systematic review and meta-analysis. BMC Cancer, 19(1), 871. https://doi.org/10.1186/ s12885-019-6055-9 google scholar
  • Risbridger, G. P., Davis, I. D., Birrell, S. N., & Tilley, W. D. (2010). Breast and prostate cancer: more similar than different. Nature reviews. Cancer, 10(3), 205-212. https://doi.org/10.1038/nrc2795 google scholar
  • Selvakumar, P., Lakshmikuttyamma, A., Das, U., Pati, H. N., Dim-mock, J. R., & Sharma, R. K. (2009). NC2213: a novel methionine aminopeptidase 2 inhibitor in human colon cancer HT29 cells. Molecular cancer, 8(65). https://doi.org/10.1186/1476-4598-8-65 Selvakumar, P., Lakshmikuttyamma, A., Dimmock, J. R., & Sharma, R. K. (2006). Methionine aminopeptidase 2 and cancer. Biochimica et biophysica acta, 1765(2), 148-154. https://doi.org/10.1016/j. bbcan.2005.11.001 google scholar
  • Sun, Y. S., Zhao, Z., Yang, Z. N., Xu, F., Lu, H. J., Zhu, Z. Y.,... Zhu, H. P. (2017). Risk Factors and Preventions of Breast Cancer. International journal of biological sciences, 13(11), 1387-1397. https://doi. org/10.7150/ijbs.21635 google scholar
  • Yamaoka, M., Yamamoto, T., Ikeyama, S., Sudo, K., & Fujita, T. (1993). Angiogenesis inhibitor TNP-470 (AGM-1470) potently inhibits the tumor growth of hormone-independent human breast and prostate carcinoma cell lines. Cancer research, 53(21), 5233-5236. Yılmaz, Ö., Bayer, B., Bekçi, H., Uba, A. I., Cumaoğlu, A., Yelekçi, K., & Küçükgüzel, Ş. G. (2020). Synthesis, Anticancer Activity on Prostate Cancer Cell Lines and Molecular Modeling Studies of Flurbi-profen-Thioether Derivatives as Potential Target of MetAP (Type II). Medicinal chemistry, 16(6), 735-749. https://doi.org/10.2174/1 573406415666190613162322 google scholar

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APA

Atlıhan, İ., Koçyiğit Sevinç, S., Orun, O., Yılmaz, Ö., Güniz Küçükgüzel, Ş., & Mega Tiber, P. (2022). Effect of flurbiprofen derivative (SGK597) on cell proliferation and apoptosis of breast cancer cell lines. İstanbul Journal of Pharmacy, 52(3), 265-270. https://doi.org/10.26650/IstanbulJPharm.2022.1094112


AMA

Atlıhan İ, Koçyiğit Sevinç S, Orun O, Yılmaz Ö, Güniz Küçükgüzel Ş, Mega Tiber P. Effect of flurbiprofen derivative (SGK597) on cell proliferation and apoptosis of breast cancer cell lines. İstanbul Journal of Pharmacy. 2022;52(3):265-270. https://doi.org/10.26650/IstanbulJPharm.2022.1094112


ABNT

Atlıhan, İ.; Koçyiğit Sevinç, S.; Orun, O.; Yılmaz, Ö.; Güniz Küçükgüzel, Ş.; Mega Tiber, P. Effect of flurbiprofen derivative (SGK597) on cell proliferation and apoptosis of breast cancer cell lines. İstanbul Journal of Pharmacy, [Publisher Location], v. 52, n. 3, p. 265-270, 2022.


Chicago: Author-Date Style

Atlıhan, İrem, and Sevgi Koçyiğit Sevinç and Oya Orun and Özgür Yılmaz and Şükriye Güniz Küçükgüzel and Pınar Mega Tiber. 2022. “Effect of flurbiprofen derivative (SGK597) on cell proliferation and apoptosis of breast cancer cell lines.” İstanbul Journal of Pharmacy 52, no. 3: 265-270. https://doi.org/10.26650/IstanbulJPharm.2022.1094112


Chicago: Humanities Style

Atlıhan, İrem, and Sevgi Koçyiğit Sevinç and Oya Orun and Özgür Yılmaz and Şükriye Güniz Küçükgüzel and Pınar Mega Tiber. “Effect of flurbiprofen derivative (SGK597) on cell proliferation and apoptosis of breast cancer cell lines.” İstanbul Journal of Pharmacy 52, no. 3 (Jun. 2023): 265-270. https://doi.org/10.26650/IstanbulJPharm.2022.1094112


Harvard: Australian Style

Atlıhan, İ & Koçyiğit Sevinç, S & Orun, O & Yılmaz, Ö & Güniz Küçükgüzel, Ş & Mega Tiber, P 2022, 'Effect of flurbiprofen derivative (SGK597) on cell proliferation and apoptosis of breast cancer cell lines', İstanbul Journal of Pharmacy, vol. 52, no. 3, pp. 265-270, viewed 7 Jun. 2023, https://doi.org/10.26650/IstanbulJPharm.2022.1094112


Harvard: Author-Date Style

Atlıhan, İ. and Koçyiğit Sevinç, S. and Orun, O. and Yılmaz, Ö. and Güniz Küçükgüzel, Ş. and Mega Tiber, P. (2022) ‘Effect of flurbiprofen derivative (SGK597) on cell proliferation and apoptosis of breast cancer cell lines’, İstanbul Journal of Pharmacy, 52(3), pp. 265-270. https://doi.org/10.26650/IstanbulJPharm.2022.1094112 (7 Jun. 2023).


MLA

Atlıhan, İrem, and Sevgi Koçyiğit Sevinç and Oya Orun and Özgür Yılmaz and Şükriye Güniz Küçükgüzel and Pınar Mega Tiber. “Effect of flurbiprofen derivative (SGK597) on cell proliferation and apoptosis of breast cancer cell lines.” İstanbul Journal of Pharmacy, vol. 52, no. 3, 2022, pp. 265-270. [Database Container], https://doi.org/10.26650/IstanbulJPharm.2022.1094112


Vancouver

Atlıhan İ, Koçyiğit Sevinç S, Orun O, Yılmaz Ö, Güniz Küçükgüzel Ş, Mega Tiber P. Effect of flurbiprofen derivative (SGK597) on cell proliferation and apoptosis of breast cancer cell lines. İstanbul Journal of Pharmacy [Internet]. 7 Jun. 2023 [cited 7 Jun. 2023];52(3):265-270. Available from: https://doi.org/10.26650/IstanbulJPharm.2022.1094112 doi: 10.26650/IstanbulJPharm.2022.1094112


ISNAD

Atlıhan, İrem - Koçyiğit Sevinç, Sevgi - Orun, Oya - Yılmaz, Özgür - Güniz Küçükgüzel, Şükriye - Mega Tiber, Pınar. “Effect of flurbiprofen derivative (SGK597) on cell proliferation and apoptosis of breast cancer cell lines”. İstanbul Journal of Pharmacy 52/3 (Jun. 2023): 265-270. https://doi.org/10.26650/IstanbulJPharm.2022.1094112



ZAMAN ÇİZELGESİ


Gönderim30.03.2022
Kabul22.09.2022
Çevrimiçi Yayınlanma30.12.2022

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