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DOI :10.26650/IstanbulJPharm.2022.1105443   IUP :10.26650/IstanbulJPharm.2022.1105443    Tam Metin (PDF)

Effects of thymoquinone and etoposide combination on cell viability and genotoxicity in human cervical cancer hela cells

Hediye Gamze Nur ÇelebioğluMerve BecitAydan ÇağlayanSevtap Aydın Dilsiz

Background and Aims: It is thought that thymoquinone might have a crucial role in preventing DNA damage, regulating DNA repair mechanisms, and inhibiting the formation of a cancer. Studies on the cytotoxic and genotoxic effects of thymoquinone together with etoposide in cervical carcinoma cells (HeLa) are not adequate. The objective of this study is to evaluate the effect of combinations with thymoquinone on etoposide cytotoxicity and genotoxicity in HeLa cells. Methods: Cytotoxicity was evaluated by MTT assay and genotoxicity was determined by Comet assay. Results: The IC50 values of thymoquinone were 233.6 μM and 145.5 μM, and the IC50 values of etoposide were 167.3 μM and 52.7 μM for 24 and 48 h, respectively. Thymoquinone significantly decreased the approximate IC50 value of etoposide in doses of 15.63 μM and above for 24 h and 31.5 μM and above for 48 h in a dose-dependent manner. 0.1-5 μM thymoquinone and 1 μM etoposide alone did not cause DNA damage, but at higher doses increased DNA damage significantly in a dose-dependent manner. Thymoquinone significantly reduced DNA damage induced by 10 μM etoposide at the doses of 0.1-10 μM. Conclusion: Our results show that thymoquinone might increase the cytotoxic and genotoxic effects of etoposide in HeLa cells at high doses and reduce DNA damage at low doses that are not cytotoxic, which suggests that etoposide may increase its anticancer effect at high doses, but comprehensive studies are needed on this subject. This study is a preliminary study and will contribute to the development of new treatment strategies.


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APA

Nur Çelebioğlu, H.G., Becit, M., Çağlayan, A., & Aydın Dilsiz, S. (2022). Effects of thymoquinone and etoposide combination on cell viability and genotoxicity in human cervical cancer hela cells. İstanbul Journal of Pharmacy, 52(3), 258-264. https://doi.org/10.26650/IstanbulJPharm.2022.1105443


AMA

Nur Çelebioğlu H G, Becit M, Çağlayan A, Aydın Dilsiz S. Effects of thymoquinone and etoposide combination on cell viability and genotoxicity in human cervical cancer hela cells. İstanbul Journal of Pharmacy. 2022;52(3):258-264. https://doi.org/10.26650/IstanbulJPharm.2022.1105443


ABNT

Nur Çelebioğlu, H.G.; Becit, M.; Çağlayan, A.; Aydın Dilsiz, S. Effects of thymoquinone and etoposide combination on cell viability and genotoxicity in human cervical cancer hela cells. İstanbul Journal of Pharmacy, [Publisher Location], v. 52, n. 3, p. 258-264, 2022.


Chicago: Author-Date Style

Nur Çelebioğlu, Hediye Gamze, and Merve Becit and Aydan Çağlayan and Sevtap Aydın Dilsiz. 2022. “Effects of thymoquinone and etoposide combination on cell viability and genotoxicity in human cervical cancer hela cells.” İstanbul Journal of Pharmacy 52, no. 3: 258-264. https://doi.org/10.26650/IstanbulJPharm.2022.1105443


Chicago: Humanities Style

Nur Çelebioğlu, Hediye Gamze, and Merve Becit and Aydan Çağlayan and Sevtap Aydın Dilsiz. Effects of thymoquinone and etoposide combination on cell viability and genotoxicity in human cervical cancer hela cells.” İstanbul Journal of Pharmacy 52, no. 3 (Jun. 2023): 258-264. https://doi.org/10.26650/IstanbulJPharm.2022.1105443


Harvard: Australian Style

Nur Çelebioğlu, HG & Becit, M & Çağlayan, A & Aydın Dilsiz, S 2022, 'Effects of thymoquinone and etoposide combination on cell viability and genotoxicity in human cervical cancer hela cells', İstanbul Journal of Pharmacy, vol. 52, no. 3, pp. 258-264, viewed 7 Jun. 2023, https://doi.org/10.26650/IstanbulJPharm.2022.1105443


Harvard: Author-Date Style

Nur Çelebioğlu, H.G. and Becit, M. and Çağlayan, A. and Aydın Dilsiz, S. (2022) ‘Effects of thymoquinone and etoposide combination on cell viability and genotoxicity in human cervical cancer hela cells’, İstanbul Journal of Pharmacy, 52(3), pp. 258-264. https://doi.org/10.26650/IstanbulJPharm.2022.1105443 (7 Jun. 2023).


MLA

Nur Çelebioğlu, Hediye Gamze, and Merve Becit and Aydan Çağlayan and Sevtap Aydın Dilsiz. Effects of thymoquinone and etoposide combination on cell viability and genotoxicity in human cervical cancer hela cells.” İstanbul Journal of Pharmacy, vol. 52, no. 3, 2022, pp. 258-264. [Database Container], https://doi.org/10.26650/IstanbulJPharm.2022.1105443


Vancouver

Nur Çelebioğlu HG, Becit M, Çağlayan A, Aydın Dilsiz S. Effects of thymoquinone and etoposide combination on cell viability and genotoxicity in human cervical cancer hela cells. İstanbul Journal of Pharmacy [Internet]. 7 Jun. 2023 [cited 7 Jun. 2023];52(3):258-264. Available from: https://doi.org/10.26650/IstanbulJPharm.2022.1105443 doi: 10.26650/IstanbulJPharm.2022.1105443


ISNAD

Nur Çelebioğlu, HediyeGamze - Becit, Merve - Çağlayan, Aydan - Aydın Dilsiz, Sevtap. Effects of thymoquinone and etoposide combination on cell viability and genotoxicity in human cervical cancer hela cells”. İstanbul Journal of Pharmacy 52/3 (Jun. 2023): 258-264. https://doi.org/10.26650/IstanbulJPharm.2022.1105443



ZAMAN ÇİZELGESİ


Gönderim20.04.2022
Kabul15.08.2022
Çevrimiçi Yayınlanma30.12.2022

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