İstanbul Journal of Pharmacy

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DOI :10.26650/IstanbulJPharm.2022.1133531   IUP :10.26650/IstanbulJPharm.2022.1133531    Tam Metin (PDF)

Effects of vitamin D on proliferation, invasion and energy metabolism of MCF-7 breast cancer cell line

Emine Nedime Korucu, Ali Ünlü

Background and Aims: 1,25(OH)2D3 (vitamin D) is a pleiotropic hormone with anti-proliferative, pro-apoptotic, and prodifferentiation effects on various cell types, which suggest anti-cancer activity in addition to its classical regulatory action on calcium and phosphate metabolism. Methods: We aimed to put forward the effects of vitamin D in various concentrations and time intervals on cell proliferation and invasion of human estrogen receptor-positive breast cancer (MCF-7) cells by real-time cell electronic sensing system (xCELLigence). A determined dose of the IC50 was applied on samples taken from cell lysates and analyzed the levels of the energy. We also aimed to clarify how vitamin D effects the activity of the protease uPA and their relations with each other. Results: Vitamin D showed a cytotoxic effect on MCF-7 cells in a time and dose dependent manner, with dose of IC50 found to be 140 nM. ATP, ADP, and AMP levels, as well as uPA activities were respectively increased in vitamin D treatment group compared to the control group for the first 24 hours while decreasing at 48, 72, and 96 hours. We determined that 70 and 140 nM vitamin D were decreased in invasion of MCF-7 cells compared to control cells. Conclusion: We observed that proliferation and invasion of breast cancer cells were inhibited by vitamin D treatment on a dose and time dependent manner, and also vitamin D supplementation decreased uPA activity and energy levels. Further studies on the mechanisms of vitamin D and the formulation of none-hypercalcemic analogues in featured are needed.

Anahtar Kelimeler: Activity of uPA, Energy levels, Invasion, Proliferation, Vitamin D

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APA

Korucu, E.N., & Ünlü, A. (2022). Effects of vitamin D on proliferation, invasion and energy metabolism of MCF-7 breast cancer cell line. İstanbul Journal of Pharmacy, 52(3), 311-317. https://doi.org/10.26650/IstanbulJPharm.2022.1133531


AMA

Korucu E N, Ünlü A. Effects of vitamin D on proliferation, invasion and energy metabolism of MCF-7 breast cancer cell line. İstanbul Journal of Pharmacy. 2022;52(3):311-317. https://doi.org/10.26650/IstanbulJPharm.2022.1133531


ABNT

Korucu, E.N.; Ünlü, A. Effects of vitamin D on proliferation, invasion and energy metabolism of MCF-7 breast cancer cell line. İstanbul Journal of Pharmacy, [Publisher Location], v. 52, n. 3, p. 311-317, 2022.


Chicago: Author-Date Style

Korucu, Emine Nedime, and Ali Ünlü. 2022. “Effects of vitamin D on proliferation, invasion and energy metabolism of MCF-7 breast cancer cell line.” İstanbul Journal of Pharmacy 52, no. 3: 311-317. https://doi.org/10.26650/IstanbulJPharm.2022.1133531


Chicago: Humanities Style

Korucu, Emine Nedime, and Ali Ünlü. “Effects of vitamin D on proliferation, invasion and energy metabolism of MCF-7 breast cancer cell line.” İstanbul Journal of Pharmacy 52, no. 3 (Jun. 2023): 311-317. https://doi.org/10.26650/IstanbulJPharm.2022.1133531


Harvard: Australian Style

Korucu, EN & Ünlü, A 2022, 'Effects of vitamin D on proliferation, invasion and energy metabolism of MCF-7 breast cancer cell line', İstanbul Journal of Pharmacy, vol. 52, no. 3, pp. 311-317, viewed 9 Jun. 2023, https://doi.org/10.26650/IstanbulJPharm.2022.1133531


Harvard: Author-Date Style

Korucu, E.N. and Ünlü, A. (2022) ‘Effects of vitamin D on proliferation, invasion and energy metabolism of MCF-7 breast cancer cell line’, İstanbul Journal of Pharmacy, 52(3), pp. 311-317. https://doi.org/10.26650/IstanbulJPharm.2022.1133531 (9 Jun. 2023).


MLA

Korucu, Emine Nedime, and Ali Ünlü. “Effects of vitamin D on proliferation, invasion and energy metabolism of MCF-7 breast cancer cell line.” İstanbul Journal of Pharmacy, vol. 52, no. 3, 2022, pp. 311-317. [Database Container], https://doi.org/10.26650/IstanbulJPharm.2022.1133531


Vancouver

Korucu EN, Ünlü A. Effects of vitamin D on proliferation, invasion and energy metabolism of MCF-7 breast cancer cell line. İstanbul Journal of Pharmacy [Internet]. 9 Jun. 2023 [cited 9 Jun. 2023];52(3):311-317. Available from: https://doi.org/10.26650/IstanbulJPharm.2022.1133531 doi: 10.26650/IstanbulJPharm.2022.1133531


ISNAD

Korucu, EmineNedime - Ünlü, Ali. “Effects of vitamin D on proliferation, invasion and energy metabolism of MCF-7 breast cancer cell line”. İstanbul Journal of Pharmacy 52/3 (Jun. 2023): 311-317. https://doi.org/10.26650/IstanbulJPharm.2022.1133531



ZAMAN ÇİZELGESİ


Gönderim21.06.2022
Kabul28.11.2022
Çevrimiçi Yayınlanma30.12.2022

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