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DOI :10.26650/IstanbulJPharm.2019.19021   IUP :10.26650/IstanbulJPharm.2019.19021    Tam Metin (PDF)

In vitro evaluation of Rheum ribes induced genotoxicity in HepG2 cell lines

Mahmoud Abudayyak

Rheum ribes is a perennial herbaceous plant belonging to the Polygonaceae family that grows more on rocky and gravelly slopes in high altitude areas of Levant and Turkey. Rheum ribes is consumed as food and widely used in folk medicine against nausea, constipation and for different diseases including diabetes and hypertension. Unfortunately, the research on Rheum ribes toxicity is insufficient. In our study, the human hepatocellular carcinoma (HepG2) cell line was used in a cytotoxicity evaluation of Rheum ribes water, methanol and chloroform extracts by MTT and NRU tests. Comet assay was used to investigate the genotoxicity potentials of the plant extracts. Our results show that all extracts cause cell death in a concentration dependent manner at 5-50 mg/mL concentrations. The IC50 values are 14.29-31.94 mg/mL by MTT and 21.15-27.66 mg/mL by NRU assay. The highest concentration (25 mg/mL) of methanol extract causes significant DNA damage (8.7-folds). In conclusion, similar to a lot of plants used in folk medicine the risk of Rheum ribes is still unknown. The uncontrolled use of this plant could cause harm to the patients. Our results indicate the possible cyto- and gentoxicity effects of Rheum ribes, these results should elevate concerns about the safety of Rheum ribes and other folk herbs. 


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DIŞA AKTAR



APA

Abudayyak, M. (2019). In vitro evaluation of Rheum ribes induced genotoxicity in HepG2 cell lines. İstanbul Journal of Pharmacy, 49(3), 132-136. https://doi.org/10.26650/IstanbulJPharm.2019.19021


AMA

Abudayyak M. In vitro evaluation of Rheum ribes induced genotoxicity in HepG2 cell lines. İstanbul Journal of Pharmacy. 2019;49(3):132-136. https://doi.org/10.26650/IstanbulJPharm.2019.19021


ABNT

Abudayyak, M. In vitro evaluation of Rheum ribes induced genotoxicity in HepG2 cell lines. İstanbul Journal of Pharmacy, [Publisher Location], v. 49, n. 3, p. 132-136, 2019.


Chicago: Author-Date Style

Abudayyak, Mahmoud,. 2019. “In vitro evaluation of Rheum ribes induced genotoxicity in HepG2 cell lines.” İstanbul Journal of Pharmacy 49, no. 3: 132-136. https://doi.org/10.26650/IstanbulJPharm.2019.19021


Chicago: Humanities Style

Abudayyak, Mahmoud,. In vitro evaluation of Rheum ribes induced genotoxicity in HepG2 cell lines.” İstanbul Journal of Pharmacy 49, no. 3 (Feb. 2023): 132-136. https://doi.org/10.26650/IstanbulJPharm.2019.19021


Harvard: Australian Style

Abudayyak, M 2019, 'In vitro evaluation of Rheum ribes induced genotoxicity in HepG2 cell lines', İstanbul Journal of Pharmacy, vol. 49, no. 3, pp. 132-136, viewed 4 Feb. 2023, https://doi.org/10.26650/IstanbulJPharm.2019.19021


Harvard: Author-Date Style

Abudayyak, M. (2019) ‘In vitro evaluation of Rheum ribes induced genotoxicity in HepG2 cell lines’, İstanbul Journal of Pharmacy, 49(3), pp. 132-136. https://doi.org/10.26650/IstanbulJPharm.2019.19021 (4 Feb. 2023).


MLA

Abudayyak, Mahmoud,. In vitro evaluation of Rheum ribes induced genotoxicity in HepG2 cell lines.” İstanbul Journal of Pharmacy, vol. 49, no. 3, 2019, pp. 132-136. [Database Container], https://doi.org/10.26650/IstanbulJPharm.2019.19021


Vancouver

Abudayyak M. In vitro evaluation of Rheum ribes induced genotoxicity in HepG2 cell lines. İstanbul Journal of Pharmacy [Internet]. 4 Feb. 2023 [cited 4 Feb. 2023];49(3):132-136. Available from: https://doi.org/10.26650/IstanbulJPharm.2019.19021 doi: 10.26650/IstanbulJPharm.2019.19021


ISNAD

Abudayyak, Mahmoud. In vitro evaluation of Rheum ribes induced genotoxicity in HepG2 cell lines”. İstanbul Journal of Pharmacy 49/3 (Feb. 2023): 132-136. https://doi.org/10.26650/IstanbulJPharm.2019.19021



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Kabul26.07.2019

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