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DOI :10.26650/IstanbulJPharm.2019.19035   IUP :10.26650/IstanbulJPharm.2019.19035    Tam Metin (PDF)

Investigation of antioxidant and cytotoxic activities of Opuntia ficus-indica (L.) Mill. fruit extract

Mehmet Evren OkurNihal KarakaşAyşe Esra KaradağDamla UludağDerya Çiçek Polat

Opuntia ficus-indica fruits are claimed to possess several health-beneficial properties. This study was carried out to determine the phytochemicals as well as to compare antioxidant activity and cell viability of the methanol extract of OFI-fruits. Antioxidant activity was evaluated by various in vitro tests such as DPPH and ABTS. LC-MS and HPLC analyses were then performed. The activity of the OFI-fruit extract on viability of U87-MG (glioblastoma multiform), HT-29 (colon cancer) and human primary fibroblast cells was investigated by determining viable cells on a luciferase based system (Cell TiterGlo; Promega; USA). The results showed that OFI-fruits were a source of antioxidant compounds. Moreover, the extract was found to have rich phenolic-flavonoid contents. Quercetin was found in the extract according to LC-MS and HPLC studies. A remarkable increased cell death in the HT-29 and U87 cell lines was detected in a concentration-dependent manner (1-10mg/mL). Among these cell lines, HT-29 responded to OFI-fruit treatment more sensitively to even the lowest dose (1mg/mL). The results of this study showed that the OFI-fruit has significant antioxidant capacity and leads to strong cytotoxic activity on various cancer cells. Therefore, it can be suggested that OFI-fruits may have chemo-preventive potential and can be studied further to clarify cytotoxic abilities.


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DIŞA AKTAR



APA

Okur, M., Karakaş, N., Karadağ, A., Uludağ, D., & Polat, D. (2019). Investigation of antioxidant and cytotoxic activities of Opuntia ficus-indica (L.) Mill. fruit extract. İstanbul Journal of Pharmacy, 49(3), 154-160. https://doi.org/10.26650/IstanbulJPharm.2019.19035


AMA

Okur M, Karakaş N, Karadağ A, Uludağ D, Polat D. Investigation of antioxidant and cytotoxic activities of Opuntia ficus-indica (L.) Mill. fruit extract. İstanbul Journal of Pharmacy. 2019;49(3):154-160. https://doi.org/10.26650/IstanbulJPharm.2019.19035


ABNT

Okur, M.; Karakaş, N.; Karadağ, A.; Uludağ, D.; Polat, D. Investigation of antioxidant and cytotoxic activities of Opuntia ficus-indica (L.) Mill. fruit extract. İstanbul Journal of Pharmacy, [Publisher Location], v. 49, n. 3, p. 154-160, 2019.


Chicago: Author-Date Style

Okur, Mehmet Evren, and Nihal Karakaş and Ayşe Esra Karadağ and Damla Uludağ and Derya Çiçek Polat. 2019. “Investigation of antioxidant and cytotoxic activities of Opuntia ficus-indica (L.) Mill. fruit extract.” İstanbul Journal of Pharmacy 49, no. 3: 154-160. https://doi.org/10.26650/IstanbulJPharm.2019.19035


Chicago: Humanities Style

Okur, Mehmet Evren, and Nihal Karakaş and Ayşe Esra Karadağ and Damla Uludağ and Derya Çiçek Polat. Investigation of antioxidant and cytotoxic activities of Opuntia ficus-indica (L.) Mill. fruit extract.” İstanbul Journal of Pharmacy 49, no. 3 (Feb. 2023): 154-160. https://doi.org/10.26650/IstanbulJPharm.2019.19035


Harvard: Australian Style

Okur, M & Karakaş, N & Karadağ, A & Uludağ, D & Polat, D 2019, 'Investigation of antioxidant and cytotoxic activities of Opuntia ficus-indica (L.) Mill. fruit extract', İstanbul Journal of Pharmacy, vol. 49, no. 3, pp. 154-160, viewed 4 Feb. 2023, https://doi.org/10.26650/IstanbulJPharm.2019.19035


Harvard: Author-Date Style

Okur, M. and Karakaş, N. and Karadağ, A. and Uludağ, D. and Polat, D. (2019) ‘Investigation of antioxidant and cytotoxic activities of Opuntia ficus-indica (L.) Mill. fruit extract’, İstanbul Journal of Pharmacy, 49(3), pp. 154-160. https://doi.org/10.26650/IstanbulJPharm.2019.19035 (4 Feb. 2023).


MLA

Okur, Mehmet Evren, and Nihal Karakaş and Ayşe Esra Karadağ and Damla Uludağ and Derya Çiçek Polat. Investigation of antioxidant and cytotoxic activities of Opuntia ficus-indica (L.) Mill. fruit extract.” İstanbul Journal of Pharmacy, vol. 49, no. 3, 2019, pp. 154-160. [Database Container], https://doi.org/10.26650/IstanbulJPharm.2019.19035


Vancouver

Okur M, Karakaş N, Karadağ A, Uludağ D, Polat D. Investigation of antioxidant and cytotoxic activities of Opuntia ficus-indica (L.) Mill. fruit extract. İstanbul Journal of Pharmacy [Internet]. 4 Feb. 2023 [cited 4 Feb. 2023];49(3):154-160. Available from: https://doi.org/10.26650/IstanbulJPharm.2019.19035 doi: 10.26650/IstanbulJPharm.2019.19035


ISNAD

Okur, Mehmet Evren - Karakaş, Nihal - Karadağ, Ayşe Esra - Uludağ, Damla - Polat, Derya Çiçek. Investigation of antioxidant and cytotoxic activities of Opuntia ficus-indica (L.) Mill. fruit extract”. İstanbul Journal of Pharmacy 49/3 (Feb. 2023): 154-160. https://doi.org/10.26650/IstanbulJPharm.2019.19035



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Kabul28.08.2019

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