Protective effects of Brenania brieyi (De Wild)  E.M.A.Petit root bark fractions against inflammatory-mediated hemolysis and dyslipidemia in rats

Chukwuma Ifeoma; Nworah Florence; Nwafor Felix; Ezeanyika Lawrence; Ogugua Victor

doi:https://dx.doi.org/10.26650/IstanbulJPharm.2022.1007696

Araştırma Makalesi

DOI :10.26650/IstanbulJPharm.2022.1007696 IUP :10.26650/IstanbulJPharm.2022.1007696 Tam Metin (PDF)

Protective effects of Brenania brieyi (De Wild) E.M.A.Petit root bark fractions against inflammatory-mediated hemolysis and dyslipidemia in rats

Ifeoma Felicia Chukwuma, Victor Onukwube Apeh, Florence Nkechi Nworah, Felix Ifeanyi Nwafor, Lawrence Uchenna Sunday Ezeanyika, Victor Nwadiogo Ogugua

Background and Aims: The inflammatory response, though protective, is the major cause of debilitating diseases when provoked excessively or if left unresolved. Brenania brieyi (De Wild) E.M.A.Petit is widely used in folk medicine for the treatment of inflammatory-related diseases. This study investigated the protective effects of methanol and chloroform root bark fractions of Brenania brieyi on inflammation-induced hemolysis and dyslipidemia. Methods: Anti-inflammatory activity was investigated by inserting 20 mg of autoclaved cotton pellets into forty-five rats randomly distributed into nine groups (n=5), this excluded group 1 (baseline). The extent of hemolysis and dyslipidemia in the inflamed rats was ascertained from hematological parameters, lipid profile, and lipidemic index, while the possible underlying mechanisms of inflammation were determined using standard procedures. Results: Treatment with varying doses of the root bark fractions of B. brieyi elicited a significant (p< 0.05) decrease in granuloma tissue and an increase (p<0.05) in hemoglobin, red and white blood cell count, packed cell volume, and platelets compared with the untreated group 2. A significant (p<0.05) decrease in cholesterol, triacylglycerols, and low-density lipoprotein, and a non-significant (p>0.05) increase in high-density lipoprotein were observed in almost all the test groups compared with group 2. There was a significant restoration of atherogenic and dyslipidemia indices and inhibition of acetic acid-induced vascular permeability, membrane hemolysis, and platelet aggregation in the fraction-treated groups compared with the control. Conclusion: The findings from this study suggest that B. brieyi inhibits exudation and proliferation of granuloma-forming cells and also has the potential to restore the hematological parameters and lipid anomalies to their physiologic state under chronic inflammation. The possible mechanisms of its action could be inhibition of vascular permeability, stabilization of the membrane, or inhibition of platelet aggregation. This justifies the use of the plant in traditional medicine and also demonstrates its potential as a target for the discovery of new anti-inflammatory agents.

Anahtar Kelimeler: Acute toxicity, Anti-inflammatory activity, Brenania brieyi, Chronic inflammation, Hematological parameters, Platelet aggregation, Lipid profile

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DIŞA AKTAR

APA

Chukwuma, I.F., Apeh, V.O., Nworah, F.N., Nwafor, F.I., Ezeanyika, L.U., & Ogugua, V.N. (2022). Protective effects of Brenania brieyi (De Wild) E.M.A.Petit root bark fractions against inflammatory-mediated hemolysis and dyslipidemia in rats. İstanbul Journal of Pharmacy, 52(3), 271-280. https://doi.org/10.26650/IstanbulJPharm.2022.1007696

AMA

Chukwuma I F, Apeh V O, Nworah F N, Nwafor F I, Ezeanyika L U, Ogugua V N. Protective effects of Brenania brieyi (De Wild) E.M.A.Petit root bark fractions against inflammatory-mediated hemolysis and dyslipidemia in rats. İstanbul Journal of Pharmacy. 2022;52(3):271-280. https://doi.org/10.26650/IstanbulJPharm.2022.1007696

ABNT

Chukwuma, I.F.; Apeh, V.O.; Nworah, F.N.; Nwafor, F.I.; Ezeanyika, L.U.; Ogugua, V.N. Protective effects of Brenania brieyi (De Wild) E.M.A.Petit root bark fractions against inflammatory-mediated hemolysis and dyslipidemia in rats. İstanbul Journal of Pharmacy, [Publisher Location], v. 52, n. 3, p. 271-280, 2022.

Chicago: Author-Date Style

Chukwuma, Ifeoma Felicia, and Victor Onukwube Apeh and Florence Nkechi Nworah and Felix Ifeanyi Nwafor and Lawrence Uchenna Sunday Ezeanyika and Victor Nwadiogo Ogugua. 2022. “Protective effects of Brenania brieyi (De Wild) E.M.A.Petit root bark fractions against inflammatory-mediated hemolysis and dyslipidemia in rats.” İstanbul Journal of Pharmacy 52, no. 3: 271-280. https://doi.org/10.26650/IstanbulJPharm.2022.1007696

Chicago: Humanities Style

Chukwuma, Ifeoma Felicia, and Victor Onukwube Apeh and Florence Nkechi Nworah and Felix Ifeanyi Nwafor and Lawrence Uchenna Sunday Ezeanyika and Victor Nwadiogo Ogugua. “Protective effects of Brenania brieyi (De Wild) E.M.A.Petit root bark fractions against inflammatory-mediated hemolysis and dyslipidemia in rats.” İstanbul Journal of Pharmacy 52, no. 3 (Jun. 2023): 271-280. https://doi.org/10.26650/IstanbulJPharm.2022.1007696

Harvard: Australian Style

Chukwuma, IF & Apeh, VO & Nworah, FN & Nwafor, FI & Ezeanyika, LU & Ogugua, VN 2022, 'Protective effects of Brenania brieyi (De Wild) E.M.A.Petit root bark fractions against inflammatory-mediated hemolysis and dyslipidemia in rats', İstanbul Journal of Pharmacy, vol. 52, no. 3, pp. 271-280, viewed 7 Jun. 2023, https://doi.org/10.26650/IstanbulJPharm.2022.1007696

Harvard: Author-Date Style

Chukwuma, I.F. and Apeh, V.O. and Nworah, F.N. and Nwafor, F.I. and Ezeanyika, L.U. and Ogugua, V.N. (2022) ‘Protective effects of Brenania brieyi (De Wild) E.M.A.Petit root bark fractions against inflammatory-mediated hemolysis and dyslipidemia in rats’, İstanbul Journal of Pharmacy, 52(3), pp. 271-280. https://doi.org/10.26650/IstanbulJPharm.2022.1007696 (7 Jun. 2023).

MLA

Chukwuma, Ifeoma Felicia, and Victor Onukwube Apeh and Florence Nkechi Nworah and Felix Ifeanyi Nwafor and Lawrence Uchenna Sunday Ezeanyika and Victor Nwadiogo Ogugua. “Protective effects of Brenania brieyi (De Wild) E.M.A.Petit root bark fractions against inflammatory-mediated hemolysis and dyslipidemia in rats.” İstanbul Journal of Pharmacy, vol. 52, no. 3, 2022, pp. 271-280. [Database Container], https://doi.org/10.26650/IstanbulJPharm.2022.1007696

Vancouver

Chukwuma IF, Apeh VO, Nworah FN, Nwafor FI, Ezeanyika LU, Ogugua VN. Protective effects of Brenania brieyi (De Wild) E.M.A.Petit root bark fractions against inflammatory-mediated hemolysis and dyslipidemia in rats. İstanbul Journal of Pharmacy [Internet]. 7 Jun. 2023 [cited 7 Jun. 2023];52(3):271-280. Available from: https://doi.org/10.26650/IstanbulJPharm.2022.1007696 doi: 10.26650/IstanbulJPharm.2022.1007696

ISNAD

Chukwuma, IfeomaFelicia - Apeh, VictorOnukwube - Nworah, FlorenceNkechi - Nwafor, FelixIfeanyi - Ezeanyika, LawrenceUchenna Sunday - Ogugua, VictorNwadiogo. “Protective effects of Brenania brieyi (De Wild) E.M.A.Petit root bark fractions against inflammatory-mediated hemolysis and dyslipidemia in rats”. İstanbul Journal of Pharmacy 52/3 (Jun. 2023): 271-280. https://doi.org/10.26650/IstanbulJPharm.2022.1007696

Cilt 52, Sayı 32022, S. 271-280

ZAMAN ÇİZELGESİ

Gönderim	12.10.2021
Kabul	22.09.2022
Çevrimiçi Yayınlanma	30.12.2022

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