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DOI :10.26650/IstanbulJPharm.2022.979195   IUP :10.26650/IstanbulJPharm.2022.979195    Tam Metin (PDF)

Sex-dependent effects of resveratrol and regular exercise on markers related to cellular stress in the hearts and kidneys of rats

Nur Banu BalSevtap HanSuzan Emel UsanmazMecit Orhan UludağEmine Demirel Yılmaz

Background and Aims: Regular exercise and several food supplements are recommended for a healthy lifestyle. In this study, the sex-dependent effects of resveratrol and regular exercise training on cellular stress response were investigated in the hearts and kidneys of rats. Methods: Male and female Wistar rats (3-month-old) were used. Resveratrol (7.5 mg/kg, in the drinking water) and exercise training (for 40 minutes, three days per week) were applied for 6 weeks. The markers related to cellular stress were measured in left ventricle and kidney tissues. Results: Cardiac total antioxidant capacity (TAC) levels were greater in males than females, but no difference was observed in kidney tissue. Exercise training increased both TAC and nitrite levels in the heart in females but not in males. Even cardiac nitrite levels were decreased in males by exercise. Cardiac phosphorylated-protein kinase RNA-like endoplasmic reticulum kinase (p-PERK) expression and renal glucose-regulated protein-78 (GRP78) expression were higher in the control male group compared to the female group. Cardiac tumor necrosis factor-ɑ (TNF-ɑ) expression was higher in females than males but not in kidneys. However, matrix metalloproteinase-2 (MMP-2) expression was higher in males than females in both kidney and cardiac tissues. The phospho-inhibitor κB-ɑ (p-IκB-ɑ) did not change between gender in cardiac tissues but had a decreased level in male kidneys compared to the female control group. Expression of phospho-extracellular signal-regulated kinase (p-ERK) in females and phospho-protein kinase B (p-Akt) in males were lower in kidney tissue. Resveratrol and exercise treatments markedly decreased Akt protein expression in male kidney tissue. Conclusion: Our findings indicate that the markers involved in cellular stress response were affected differently by the resveratrol and exercise treatments considering sex and tissue dependencies.

Anahtar Kelimeler: Cellular stressSexResveratrolExerciseHeartKidney

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Referanslar

  • Bairey Merz, C.N., Dember, L.M., Ingelfinger, J.R., Vinson, A., ... Star, R.A. (2019). Sex and the kidneys: Current understanding and research opportunities. Nature Reviews Nephrology, 15(12), 776 -783. http://dx.doi.org/10.1038/s41581-019-0208-6. google scholar
  • Bal, N.B., Han, S., Kiremitci, S., Uludag, M.O., & Demirel-Yilmaz, E. (2020). Reversal of deleterious effect of hypertension on the liver by inhibition of endoplasmic reticulum stress. Molecular Biology Reports, 47(3), 2243-2252. http://dx.doi.org/10.1007/s11033-020-05329-2. google scholar
  • Balcı, S.S. & Pepe, H. (2012). Effects of gender, endurance training and acute exhaustive exercise on oxidative stress in the heart and skeletal muscle of the rat. Chinese Journal of Physiology, 55(4), 236 -244. http://dx.doi.org/10.4077/CJP.2012.BAA021. google scholar
  • Baylis, C. (2012). Nitric oxide synthase derangements and hypertension in kidney disease. Current Opinion in Nephrol-ogy and Hypertension, 21(1), 1-6. http://dx.doi.org/ 10.1097/ MNH.0b013e32834d54ca. google scholar
  • Campesi, I., Franconi, F., Seghieri, G. & Meloni, M. (2017). Sex-gen-der-related therapeutic approaches for cardiovascular complica-tions associated with diabetes. Pharmacological Research, 119, 195-207http://dx.doi.org/10.1016/j.phrs.2017.01.023. google scholar
  • Chemaly, E.R., Troncone, L. & Lebeche, D. (2018). SERCA control of cell death and survival. Cell Calcium, 69, 46-61. http://dx.doi. org/10.1016/j.ceca.2017.07.001. google scholar
  • Chen, Y., Zhang, Y., Shan, M., Zhou, Y., Huang, Y. & Shi, L. (2019). Aerobic exercise-induced inhibition of PKCa/CaV1.2 pathway en-hances the vasodilation of mesenteric arteries in hypertension. Archives of Biochemistry and Biophysics,678, 108191. http://dx.doi. org/10.1016/j.abb.2019.108191. google scholar
  • Cheng, C.K., Luo, J.Y., Lau, C.W., Chen, Z.Y., Tian, X.Y. & Huang, Y. (2020). Pharmacological basis and new insights of resveratrol ac-tion in the cardiovascular system. British Journal of Pharmacology, 177(6), 1258-1277. http://dx.doi.org/10.1111/bph.14801. google scholar
  • Chengji, W. & Xianjin, F. (2019). Exercise protects against diabetic cardiomyopathy by the inhibition of the endoplasmic reticulum stress pathway in rats. Journal of Cellular Physiology, 234(2), 16821688. http://dx.doi.org/10.1002/jcp.27038. google scholar
  • Dworatzek, E., Mahmoodzadeh, S., Schubert, C., Westphal, C., . Regitz-Zagrosek V. (2014). Sex differences in exercise-induced physiological myocardial hypertrophy are modulated by oes-trogen receptor beta. Cardiovascular Research, 102(3), 418-428. http://dx.doi.org/10.1093/cvr/cvu065. google scholar
  • Erdely, A., Greenfeld, Z., Wagner, L. & Baylis, C. (2003). Sexual di-morphism in the aging kidney: Effects on injury and nitric ox-ide system. Kidney International, 63(3), 1021-1026. http://dx.doi. org/10.1046/j.1523-1755.2003.00830.x google scholar
  • EUGenMed cardiovascular clinical study group, Regitz-Zagrosek, V., Oertelt-Prigione, S., Prescott, E., . Stangl, V. (2016). Gender in cardiovascular diseases: impact on clinical manifestations, man-agement, and outcomes. European Heart Journal, 37(1), 24-34. http://dx.doi.org/10.1093/eurheartj/ehv598. google scholar
  • Farah, D., Nunes, J., Sartori, M., Dias, DD., . Angelis, K. (2016). exer-cise training prevents cardiovascular derangements induced by fructose overload in developing rats. PLoS One, 8;11(12):e0167291. http://dx.doi.org/10.1371/journal.pone.0167291. google scholar
  • Fiordelisi, A., Iaccarino, G., Morisco, C., Coscioni, E. & Sorriento, D. (2019). NFkappaB is a key player in the crosstalk between inflam-mation and cardiovascular diseases. International Journal of Molec-ular Sciences, 20(7):1599. http://dx.doi.org/10.3390/ijms20071599. google scholar
  • Gliemann, L., Nyberg, M. & Hellsten, Y. (2016). Effects of exercise training and Resveratrol on vascular health in aging. Free Radical Biology and Medicine, 98, 165-176. http://dx.doi.org/10.1016/j.fre-eradbiomed.2016.03.037. google scholar
  • Han, S., Bal, N.B., Sadi, G., Usanmaz, S.E., Uludag, M.O. & Demirel-Yilmaz, E. (2018). The effects of resveratrol and exercise on age and gender-dependent alterations of vascular functions and bio-markers. Experimental Gerontology, 110, 191-201. http://dx.doi. org/10.1016/j.exger.2018.06.009. google scholar
  • Hong, J., Kim, K., Kim, J.H. & Park, Y. (2017). The Role of Endoplas-mic Reticulum Stress in Cardiovascular Disease and Exercise. International Journal of Vascular Medicine 2017, 2049217. http:// dx.doi.org/10.1155/2017/2049217. google scholar
  • Juhasz, B., Mukherjee, S., & Das DK. (2010). Hormetic response of resveratrol against cardioprotection. Experimental & Clinical Cardi-ology, 15(4):e134-e138. google scholar
  • Katalinic, V., Modun, D., Music, I. & Boban, M. (2005). Gender dif-ferences in antioxidant capacity of rat tissues determined by 2,2’-azinobis (3-ethylbenzothiazoline 6-sulfonate; ABTS) and ferric reducing antioxidant power (FRAP) assays. Comparative Biochem-istry and Physiology - Part C: Toxicology & Pharmacology, 140(1), 47-52. http://dx.doi.Org/10.1016/j.cca.2005.01.005. google scholar
  • Kosaki, K., Sugaya, T., Ohata, K.,Tanabe, J.,... Ikemori, A. (2019). Renoprotective effects of voluntary running exercise training on aldosterone-induced renal injury in human L-FABP chromo-somal transgenic mice. Hypertension Research, 42(10), 1518-1527. http://dx.doi.org/10.1038/s41440-019-0273-z. google scholar
  • Li, H., Xia, N., & Förstermann, U. (2012). Cardiovascular effects and molecular targets of resveratrol. Nitric Oxide, 26(2), 102-110. https://doi.org/10.1016/j.niox.2011.12.006. google scholar
  • Lin Y, Zhu J, Zhang X, Wang J, . Liu, J. (2016). inhibition of cardio-myocytes hypertrophy by resveratrol is associated with ameliora-tion of endoplasmic reticulum stress. Cellular Physiology and Bio-chemistry, 39(2), 780-789. http://dx.doi.org/10.1159/000447788. google scholar
  • Liu, M., Ning, X., Li, R., Yang, Z., . Qian, Q. (2017). Signalling pathways involved in hypoxia-induced renal fibrosis. Journal of Cellular and Molecular Medicine, 21(7), 1248-1259. http://dx.doi. org/10.1111/jcmm.13060. google scholar
  • Nasiri, M., Ahmadizad, S., Hedayati, M., Zarekar, T., Seydyousefi, M., & Faghfoori Z. (2021). Trans-resveratrol supplement lowers lipid peroxidation responses of exercise in male Wistar rats. Interna-tional Journal for Vitamin and Nutrition Research, 91(5-6):507-512. http://dx.doi.org/ 10.1024/0300-9831/a000654. google scholar
  • Olesen, J., Ringholm, S., Nielsen, M.M., Brandt, C.T., . Pilegaard, H. (2013). Role of PGC-1a in exercise training- and resveratrol-induced prevention of age-associated inflammation. Experimen-tal Gerontology, 48(11), 1274-1284. http://dx.doi.org/10.1016/j. exger.2013.07.015. google scholar
  • Pektaş, M.B., Sadi, G. & Akar, F. (2015). Long-Term Dietary Fruc-tose Causes Gender-Different Metabolic and Vascular Dys-function in Rats: Modulatory Effects of Resveratrol. Cellular Physiology and Biochemistry, 37(4), 1407-1420. http://dx.doi. org/10.1159/000430405. google scholar
  • Rolim, N.P., Medeiros, A., Rosa, K.T., Mattos, K.C., . Brum, P.C. (2007). Exercise training improves the net balance of cardiac Ca2+ handling protein expression in heart failure. Physiological Genomics, 29(3), 246-252. http://dx.doi.org/10.1152/physiolge-nomics.00188.2006. google scholar
  • Saghebjoo, M., Sadeghi-Tabas, S., Saffari, I., Ghane, A2. & Dimau-ro, I. (2019). Sex Differences in antiaging response to short- and long-term high-intensity interval exercise in rat cardiac muscle: Telomerase activity, total antioxidant/oxidant status. Chinese Journal of Physiology, 62(6), 261-266. http://dx.doi.org/10.4103/ CJP.CJP_52_19. google scholar
  • Singh, A.P., Singh, R., Verma, S.S., Rai, V., . Gupta, S.C. (2019). Health benefits of resveratrol: Evidence from clinical studies. Medicinal Research Reviews, 39(5), 1851-1891. http://dx.doi. org/10.1002/med.21565. google scholar
  • Soylemez, S., Gurdal, H., Sepici, A. & Akar, F. (2008). The effect of long-term resveratrol treatment on relaxation to estrogen in aortae from male and female rats: role of nitric oxide and su-peroxide. Vascular Pharmacology, 49(2-3), 97-105. http://dx.doi. org/10.1016/j.vph.2008.06.006 google scholar
  • Soylemez, S., Sepici, A. & Akar, F. (2009). Resveratrol supplementa-tion gender independently improves endothelial reactivity and suppresses superoxide production in healthy rats. Cardiovascu-lar Drugs and Therapy, 23(6), 449-458. http://dx.doi.org/10.1007/ s10557-009-6198-z. google scholar
  • Towbin, H., Staehelin, T. & Gordon, J. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America, 76(9), 43504354. http://dx.doi.org/10.1073/pnas.76.9.4350. google scholar
  • Tung, B.T., Rodriguez-Bies, E., Thanh, H.N., Le-Thi-Thu, H., . Ldpez-Lluch, G. (2015). Organ and tissue-dependent effect of res-veratrol and exercise on antioxidant defenses of old mice. Aging Clinical and Experimental Research, 27(6), 775-783. http://dx.doi. org/10.1007/s40520-015-0366-8. google scholar
  • Usanmaz, S.E. & Demirel-Yilmaz, E. (2008). A microplate based spectrophotometric method for the determination of the total antioxidant capacity of human plasma: Modified cupric reduc-ing ability assay. Fundamental & Clinical Pharmacology, 22(Suppl. 2), 67. google scholar
  • Zhang, J., Wang, X., Vikash, V., Ye, Q., . Dong, W. (2016). ROS and ROS-Mediated cellular signaling. Oxidative Medi-cine and Cellular Longevity, 2016, 4350965. http://dx.doi. org/10.1155/2016/4350965. google scholar
  • Zhang, L., Zhang, Y., Wu, Y., Yu, J., . Shi, L. (2019). Role of the Balance of Akt and MAPK pathways in the exercise-regulated phenotype switching in spontaneously hypertensive rats. Inter-national Journal of Molecular Sciences, 20(22), 5690-5709. http:// dx.doi.org/10.3390/ijms20225690. google scholar

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APA

Bal, N.B., Han, S., Usanmaz, S.E., Uludağ, M.O., & Demirel Yılmaz, E. (2022). Sex-dependent effects of resveratrol and regular exercise on markers related to cellular stress in the hearts and kidneys of rats. İstanbul Journal of Pharmacy, 52(1), 20-30. https://doi.org/10.26650/IstanbulJPharm.2022.979195


AMA

Bal N B, Han S, Usanmaz S E, Uludağ M O, Demirel Yılmaz E. Sex-dependent effects of resveratrol and regular exercise on markers related to cellular stress in the hearts and kidneys of rats. İstanbul Journal of Pharmacy. 2022;52(1):20-30. https://doi.org/10.26650/IstanbulJPharm.2022.979195


ABNT

Bal, N.B.; Han, S.; Usanmaz, S.E.; Uludağ, M.O.; Demirel Yılmaz, E. Sex-dependent effects of resveratrol and regular exercise on markers related to cellular stress in the hearts and kidneys of rats. İstanbul Journal of Pharmacy, [Publisher Location], v. 52, n. 1, p. 20-30, 2022.


Chicago: Author-Date Style

Bal, Nur Banu, and Sevtap Han and Suzan Emel Usanmaz and Mecit Orhan Uludağ and Emine Demirel Yılmaz. 2022. “Sex-dependent effects of resveratrol and regular exercise on markers related to cellular stress in the hearts and kidneys of rats.” İstanbul Journal of Pharmacy 52, no. 1: 20-30. https://doi.org/10.26650/IstanbulJPharm.2022.979195


Chicago: Humanities Style

Bal, Nur Banu, and Sevtap Han and Suzan Emel Usanmaz and Mecit Orhan Uludağ and Emine Demirel Yılmaz. Sex-dependent effects of resveratrol and regular exercise on markers related to cellular stress in the hearts and kidneys of rats.” İstanbul Journal of Pharmacy 52, no. 1 (Jul. 2022): 20-30. https://doi.org/10.26650/IstanbulJPharm.2022.979195


Harvard: Australian Style

Bal, NB & Han, S & Usanmaz, SE & Uludağ, MO & Demirel Yılmaz, E 2022, 'Sex-dependent effects of resveratrol and regular exercise on markers related to cellular stress in the hearts and kidneys of rats', İstanbul Journal of Pharmacy, vol. 52, no. 1, pp. 20-30, viewed 4 Jul. 2022, https://doi.org/10.26650/IstanbulJPharm.2022.979195


Harvard: Author-Date Style

Bal, N.B. and Han, S. and Usanmaz, S.E. and Uludağ, M.O. and Demirel Yılmaz, E. (2022) ‘Sex-dependent effects of resveratrol and regular exercise on markers related to cellular stress in the hearts and kidneys of rats’, İstanbul Journal of Pharmacy, 52(1), pp. 20-30. https://doi.org/10.26650/IstanbulJPharm.2022.979195 (4 Jul. 2022).


MLA

Bal, Nur Banu, and Sevtap Han and Suzan Emel Usanmaz and Mecit Orhan Uludağ and Emine Demirel Yılmaz. Sex-dependent effects of resveratrol and regular exercise on markers related to cellular stress in the hearts and kidneys of rats.” İstanbul Journal of Pharmacy, vol. 52, no. 1, 2022, pp. 20-30. [Database Container], https://doi.org/10.26650/IstanbulJPharm.2022.979195


Vancouver

Bal NB, Han S, Usanmaz SE, Uludağ MO, Demirel Yılmaz E. Sex-dependent effects of resveratrol and regular exercise on markers related to cellular stress in the hearts and kidneys of rats. İstanbul Journal of Pharmacy [Internet]. 4 Jul. 2022 [cited 4 Jul. 2022];52(1):20-30. Available from: https://doi.org/10.26650/IstanbulJPharm.2022.979195 doi: 10.26650/IstanbulJPharm.2022.979195


ISNAD

Bal, NurBanu - Han, Sevtap - Usanmaz, SuzanEmel - Uludağ, MecitOrhan - Demirel Yılmaz, Emine. Sex-dependent effects of resveratrol and regular exercise on markers related to cellular stress in the hearts and kidneys of rats”. İstanbul Journal of Pharmacy 52/1 (Jul. 2022): 20-30. https://doi.org/10.26650/IstanbulJPharm.2022.979195



ZAMAN ÇİZELGESİ


Gönderim09.08.2021
Kabul15.02.2022
Çevrimiçi Yayınlanma28.04.2022

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