İstanbul Journal of Pharmacy

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DOI :10.26650/IstanbulJPharm.2023.1268463   IUP :10.26650/IstanbulJPharm.2023.1268463    Tam Metin (PDF)

The cytotoxic and apoptotic effects of Thymus vulgaris extracts on human breast cancer cell lines

Yasin Çelikok, Leyla Türker Şener, Burcu Bütün, Işıl Albeniz

Background and Aims: Plant extracts are an important source of cytotoxic compounds and have consistently been an interesting field of research. The aim of this study is to investigate the cytotoxic and apoptotic effects of Thymus vulgaris (T. vulgaris) extracts on human breast cancer cell lines.

Methods: This study was carried out using human breast cancer cell lines (MCF-7 and MDA-MB-231) as experimental groups and the healthy human fibroblast cell line (PCS-201-012) as the control group. Petroleum ether and ethanol extracts were obtained from T. vulgaris. The extracts were applied to MCF-7 and MDA-MB-231 human breast cancer cell lines and human breast cancer stem cells. Cytotoxicity studies were performed using the RTCA iCELLigence system (Agilent Technologies), and apoptosis studies were performed using terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labeling (TUNEL) and 4’,6-diamidino-2 phenylindole (DAPI) methods.

Results: The T. vulgaris extracts were found to have concentration-dependent cytotoxic effects on human breast cancer cells. The growth of breast cancer stem cells was also determined to be inhibited when an effective concentration (45 μg/ mL) of the extracts was applied. Lastly, specific morphological changes related to apoptosis were detected in the cells that had been treated with the effective concentration.

Conclusion: The T. vulgaris extracts were found to inhibit the proliferation of human breast cancer cells and human breast cancer stem cells selectively and concentration-dependently via an apoptosis-dependent pathway. The results suggest that the extracts may make promising sources for developing drugs for breast cancer therapy.

Anahtar Kelimeler: DAPI, iCELLigence, MCF-7, MDA-MB-231, Thymus vulgaris, TUNEL

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APA

Çelikok, Y., Türker Şener, L., Bütün, B., & Albeniz, I. (2023). The cytotoxic and apoptotic effects of Thymus vulgaris extracts on human breast cancer cell lines. İstanbul Journal of Pharmacy, 53(2), 186-192. https://doi.org/10.26650/IstanbulJPharm.2023.1268463


AMA

Çelikok Y, Türker Şener L, Bütün B, Albeniz I. The cytotoxic and apoptotic effects of Thymus vulgaris extracts on human breast cancer cell lines. İstanbul Journal of Pharmacy. 2023;53(2):186-192. https://doi.org/10.26650/IstanbulJPharm.2023.1268463


ABNT

Çelikok, Y.; Türker Şener, L.; Bütün, B.; Albeniz, I. The cytotoxic and apoptotic effects of Thymus vulgaris extracts on human breast cancer cell lines. İstanbul Journal of Pharmacy, [Publisher Location], v. 53, n. 2, p. 186-192, 2023.


Chicago: Author-Date Style

Çelikok, Yasin, and Leyla Türker Şener and Burcu Bütün and Işıl Albeniz. 2023. “The cytotoxic and apoptotic effects of Thymus vulgaris extracts on human breast cancer cell lines.” İstanbul Journal of Pharmacy 53, no. 2: 186-192. https://doi.org/10.26650/IstanbulJPharm.2023.1268463


Chicago: Humanities Style

Çelikok, Yasin, and Leyla Türker Şener and Burcu Bütün and Işıl Albeniz. “The cytotoxic and apoptotic effects of Thymus vulgaris extracts on human breast cancer cell lines.” İstanbul Journal of Pharmacy 53, no. 2 (Dec. 2024): 186-192. https://doi.org/10.26650/IstanbulJPharm.2023.1268463


Harvard: Australian Style

Çelikok, Y & Türker Şener, L & Bütün, B & Albeniz, I 2023, 'The cytotoxic and apoptotic effects of Thymus vulgaris extracts on human breast cancer cell lines', İstanbul Journal of Pharmacy, vol. 53, no. 2, pp. 186-192, viewed 9 Dec. 2024, https://doi.org/10.26650/IstanbulJPharm.2023.1268463


Harvard: Author-Date Style

Çelikok, Y. and Türker Şener, L. and Bütün, B. and Albeniz, I. (2023) ‘The cytotoxic and apoptotic effects of Thymus vulgaris extracts on human breast cancer cell lines’, İstanbul Journal of Pharmacy, 53(2), pp. 186-192. https://doi.org/10.26650/IstanbulJPharm.2023.1268463 (9 Dec. 2024).


MLA

Çelikok, Yasin, and Leyla Türker Şener and Burcu Bütün and Işıl Albeniz. “The cytotoxic and apoptotic effects of Thymus vulgaris extracts on human breast cancer cell lines.” İstanbul Journal of Pharmacy, vol. 53, no. 2, 2023, pp. 186-192. [Database Container], https://doi.org/10.26650/IstanbulJPharm.2023.1268463


Vancouver

Çelikok Y, Türker Şener L, Bütün B, Albeniz I. The cytotoxic and apoptotic effects of Thymus vulgaris extracts on human breast cancer cell lines. İstanbul Journal of Pharmacy [Internet]. 9 Dec. 2024 [cited 9 Dec. 2024];53(2):186-192. Available from: https://doi.org/10.26650/IstanbulJPharm.2023.1268463 doi: 10.26650/IstanbulJPharm.2023.1268463


ISNAD

Çelikok, Yasin - Türker Şener, Leyla - Bütün, Burcu - Albeniz, Işıl. “The cytotoxic and apoptotic effects of Thymus vulgaris extracts on human breast cancer cell lines”. İstanbul Journal of Pharmacy 53/2 (Dec. 2024): 186-192. https://doi.org/10.26650/IstanbulJPharm.2023.1268463



ZAMAN ÇİZELGESİ


Gönderim21.03.2023
Kabul11.06.2023
Çevrimiçi Yayınlanma28.08.2023

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