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DOI :10.26650/IstanbulJPharm.2019.19034   IUP :10.26650/IstanbulJPharm.2019.19034    Tam Metin (PDF)

The cytotoxicity and insecticidal activity of extracts from Delphinium formosum Boiss. & Huet

Burcu Şen UtsukarçiNurhayat TabancaAlden S. EstepÖzlem Akbal DağıstanSonja M. KesslerZehra ÖztürkJames BecnelAlexandra K. KiemerAfife Mat

Delphinium species are well-known toxic plants with diterpenoid alkaloid contents. There has been no previous investigation on the cytotoxicity of Delphinium formosum. The extracts of the different parts of D. formosum, an endemic species in Turkey, were investigated for their cytotoxic activity against the human liver carcinoma cell line (HepG2) and primary human umbilical vein endothelial cells (HUVEC). The cytotoxic effects of twelve extracts and subfractions were determined against HepG2 cells using the MTT assay. The only active extract was applied to the HUVEC as a model for healthy cells. Only one of the alkaloidcontaining extracts from the aerial parts was toxic (IC50=244,9 µg/mL against HepG2 and 144,4 µg/mL against HUVEC), while the root extracts were inactive. The results were improbable although it is predicted secondary metabolites, such as diterpene alkaloids (methyllycaconitine, browniine, lycoctonine, avardharidine, antranoyllycoctonine, delsemine A/B and lycoctonine). Based on previous studies in the literature, the cytotoxic plants were also expected to exhibit insecticidal activity. Therefore, the cytotoxic extract of D. formosum was examined for its adulticidal and larvicidal activity against the yellow fever, dengue fever and the Zika virus vector Aedes aegypti L.


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DIŞA AKTAR



APA

Şen Utsukarçi, B., Tabanca, N., Estep, A., Akbal Dağıstan, Ö., Kessler, S., Öztürk, Z., Becnel, J., Kiemer, A.K., & Mat, A. (2019). The cytotoxicity and insecticidal activity of extracts from Delphinium formosum Boiss. & Huet. İstanbul Journal of Pharmacy, 49(3), 148-153. https://doi.org/10.26650/IstanbulJPharm.2019.19034


AMA

Şen Utsukarçi B, Tabanca N, Estep A, Akbal Dağıstan Ö, Kessler S, Öztürk Z, Becnel J, Kiemer A K, Mat A. The cytotoxicity and insecticidal activity of extracts from Delphinium formosum Boiss. & Huet. İstanbul Journal of Pharmacy. 2019;49(3):148-153. https://doi.org/10.26650/IstanbulJPharm.2019.19034


ABNT

Şen Utsukarçi, B.; Tabanca, N.; Estep, A.; Akbal Dağıstan, Ö.; Kessler, S.; Öztürk, Z.; Becnel, J.; Kiemer, A.K.; Mat, A. The cytotoxicity and insecticidal activity of extracts from Delphinium formosum Boiss. & Huet. İstanbul Journal of Pharmacy, [Publisher Location], v. 49, n. 3, p. 148-153, 2019.


Chicago: Author-Date Style

Şen Utsukarçi, Burcu, and Nurhayat Tabanca and Alden S. Estep and Özlem Akbal Dağıstan and Sonja M. Kessler and Zehra Öztürk and James Becnel and Alexandra K. Kiemer and Afife Mat. 2019. “The cytotoxicity and insecticidal activity of extracts from Delphinium formosum Boiss. & Huet.” İstanbul Journal of Pharmacy 49, no. 3: 148-153. https://doi.org/10.26650/IstanbulJPharm.2019.19034


Chicago: Humanities Style

Şen Utsukarçi, Burcu, and Nurhayat Tabanca and Alden S. Estep and Özlem Akbal Dağıstan and Sonja M. Kessler and Zehra Öztürk and James Becnel and Alexandra K. Kiemer and Afife Mat. The cytotoxicity and insecticidal activity of extracts from Delphinium formosum Boiss. & Huet.” İstanbul Journal of Pharmacy 49, no. 3 (Feb. 2023): 148-153. https://doi.org/10.26650/IstanbulJPharm.2019.19034


Harvard: Australian Style

Şen Utsukarçi, B & Tabanca, N & Estep, A & Akbal Dağıstan, Ö & Kessler, S & Öztürk, Z & Becnel, J & Kiemer, AK & Mat, A 2019, 'The cytotoxicity and insecticidal activity of extracts from Delphinium formosum Boiss. & Huet', İstanbul Journal of Pharmacy, vol. 49, no. 3, pp. 148-153, viewed 4 Feb. 2023, https://doi.org/10.26650/IstanbulJPharm.2019.19034


Harvard: Author-Date Style

Şen Utsukarçi, B. and Tabanca, N. and Estep, A. and Akbal Dağıstan, Ö. and Kessler, S. and Öztürk, Z. and Becnel, J. and Kiemer, A.K. and Mat, A. (2019) ‘The cytotoxicity and insecticidal activity of extracts from Delphinium formosum Boiss. & Huet’, İstanbul Journal of Pharmacy, 49(3), pp. 148-153. https://doi.org/10.26650/IstanbulJPharm.2019.19034 (4 Feb. 2023).


MLA

Şen Utsukarçi, Burcu, and Nurhayat Tabanca and Alden S. Estep and Özlem Akbal Dağıstan and Sonja M. Kessler and Zehra Öztürk and James Becnel and Alexandra K. Kiemer and Afife Mat. The cytotoxicity and insecticidal activity of extracts from Delphinium formosum Boiss. & Huet.” İstanbul Journal of Pharmacy, vol. 49, no. 3, 2019, pp. 148-153. [Database Container], https://doi.org/10.26650/IstanbulJPharm.2019.19034


Vancouver

Şen Utsukarçi B, Tabanca N, Estep A, Akbal Dağıstan Ö, Kessler S, Öztürk Z, Becnel J, Kiemer AK, Mat A. The cytotoxicity and insecticidal activity of extracts from Delphinium formosum Boiss. & Huet. İstanbul Journal of Pharmacy [Internet]. 4 Feb. 2023 [cited 4 Feb. 2023];49(3):148-153. Available from: https://doi.org/10.26650/IstanbulJPharm.2019.19034 doi: 10.26650/IstanbulJPharm.2019.19034


ISNAD

Şen Utsukarçi, Burcu - Tabanca, Nurhayat - Estep, Alden S. - Akbal Dağıstan, Özlem - Kessler, Sonja M. - Öztürk, Zehra - Becnel, James - Kiemer, AlexandraK. - Mat, Afife. The cytotoxicity and insecticidal activity of extracts from Delphinium formosum Boiss. & Huet”. İstanbul Journal of Pharmacy 49/3 (Feb. 2023): 148-153. https://doi.org/10.26650/IstanbulJPharm.2019.19034



ZAMAN ÇİZELGESİ


Kabul30.09.2019

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