Aquatic Sciences and Engineering

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DOI :10.26650/ASE20241397385   IUP :10.26650/ASE20241397385    Full Text (PDF)

Phenotypic and Genotypic Antibiotic Resistance of Staphylococcus warneri and Staphylococcus pasteuri Isolated from Stuffed Mussels

Dilek Kahraman Yılmaz, Nermin Berik

This study aimed to assess the presence of Staphylococcus species in stuffed mussel samples sold in Çanakkale province and to determine the antibiotic resistance of the isolates. A total of 246 stuffed mussel samples were examined, and two different Staphylococcus (S. warneri and S. pasteuri) were isolated from 12.19% of the samples. Among the Staphylococcus isolates, S. warneri was detected at a rate of 73.33% and S. pasteuri at 26.67%. Bacterial isolates (N=30) were examined for their resistance to amoxicillin/clavulanic acid (30 µg), ampicillin (10 μg), erythromycin (15 µg), clindamycin (10 µg), vancomycin (30 µg), oxacillin (5 µg), tetracycline (30 µg), doxycycline hydrochloride (30 µg), chloramphenicol (30 µg), streptomycin (10 µg), gentamicin (10 µg), kanamycin (30 µg), nalidixic acid (30 µg), ciprofloxacin (5 µg), meropenem (10 µg), imipenem (10 µg), sulfamethox/ trimethoprim (25 µg), cefotaxime (30 µg), cephalothin (30 µg), ceftriaxone (30 µg), and levofloxacin (5 µg) antibiotics. Resistance to amoxicillin/clavulanic acid and erythromycin was found in all S. warneri isolates. In addition, all S. pasteuri isolates were found to be resistant to amoxicillin/ clavulanic acid. In S. warneri isolates, at least three resistance genes (BlaTEM, tetB-6, tetK-8) and up to eight resistance genes (BlaTEM, tetB-6, tetK-8, strA-strB, aphAI-IAB, ermC) were identified. All S. pasteuri isolates exhibited blaTEM, strA-strB, and aphAI-IAB resistance genes. In conclusion, it was determined that antibiotic-resistant S. warneri and/or S. pasteuri contaminates stuffed mussel samples. This study will serve as a valuable resource for enhancing monitoring strategies in stuffed mussel production. Further studies should be conducted to determine whether the products are suitable for food safety. Therefore, it is necessary to clarify the disease effects and mechanisms of the pathogens identified in stuffed mussels. 

Keywords: Stuffed mussel, Mytilus galloprovincialis, S. warneri, S. pasteuri, Public health, antibiotic resistance

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APA

Kahraman Yılmaz, D., & Berik, N. (2024). Phenotypic and Genotypic Antibiotic Resistance of Staphylococcus warneri and Staphylococcus pasteuri Isolated from Stuffed Mussels. Aquatic Sciences and Engineering, 39(3), 172-178. https://doi.org/10.26650/ASE20241397385


AMA

Kahraman Yılmaz D, Berik N. Phenotypic and Genotypic Antibiotic Resistance of Staphylococcus warneri and Staphylococcus pasteuri Isolated from Stuffed Mussels. Aquatic Sciences and Engineering. 2024;39(3):172-178. https://doi.org/10.26650/ASE20241397385


ABNT

Kahraman Yılmaz, D.; Berik, N. Phenotypic and Genotypic Antibiotic Resistance of Staphylococcus warneri and Staphylococcus pasteuri Isolated from Stuffed Mussels. Aquatic Sciences and Engineering, [Publisher Location], v. 39, n. 3, p. 172-178, 2024.


Chicago: Author-Date Style

Kahraman Yılmaz, Dilek, and Nermin Berik. 2024. “Phenotypic and Genotypic Antibiotic Resistance of Staphylococcus warneri and Staphylococcus pasteuri Isolated from Stuffed Mussels.” Aquatic Sciences and Engineering 39, no. 3: 172-178. https://doi.org/10.26650/ASE20241397385


Chicago: Humanities Style

Kahraman Yılmaz, Dilek, and Nermin Berik. “Phenotypic and Genotypic Antibiotic Resistance of Staphylococcus warneri and Staphylococcus pasteuri Isolated from Stuffed Mussels.” Aquatic Sciences and Engineering 39, no. 3 (Dec. 2024): 172-178. https://doi.org/10.26650/ASE20241397385


Harvard: Australian Style

Kahraman Yılmaz, D & Berik, N 2024, 'Phenotypic and Genotypic Antibiotic Resistance of Staphylococcus warneri and Staphylococcus pasteuri Isolated from Stuffed Mussels', Aquatic Sciences and Engineering, vol. 39, no. 3, pp. 172-178, viewed 23 Dec. 2024, https://doi.org/10.26650/ASE20241397385


Harvard: Author-Date Style

Kahraman Yılmaz, D. and Berik, N. (2024) ‘Phenotypic and Genotypic Antibiotic Resistance of Staphylococcus warneri and Staphylococcus pasteuri Isolated from Stuffed Mussels’, Aquatic Sciences and Engineering, 39(3), pp. 172-178. https://doi.org/10.26650/ASE20241397385 (23 Dec. 2024).


MLA

Kahraman Yılmaz, Dilek, and Nermin Berik. “Phenotypic and Genotypic Antibiotic Resistance of Staphylococcus warneri and Staphylococcus pasteuri Isolated from Stuffed Mussels.” Aquatic Sciences and Engineering, vol. 39, no. 3, 2024, pp. 172-178. [Database Container], https://doi.org/10.26650/ASE20241397385


Vancouver

Kahraman Yılmaz D, Berik N. Phenotypic and Genotypic Antibiotic Resistance of Staphylococcus warneri and Staphylococcus pasteuri Isolated from Stuffed Mussels. Aquatic Sciences and Engineering [Internet]. 23 Dec. 2024 [cited 23 Dec. 2024];39(3):172-178. Available from: https://doi.org/10.26650/ASE20241397385 doi: 10.26650/ASE20241397385


ISNAD

Kahraman Yılmaz, Dilek - Berik, Nermin. “Phenotypic and Genotypic Antibiotic Resistance of Staphylococcus warneri and Staphylococcus pasteuri Isolated from Stuffed Mussels”. Aquatic Sciences and Engineering 39/3 (Dec. 2024): 172-178. https://doi.org/10.26650/ASE20241397385



TIMELINE


Submitted28.11.2023
Accepted02.02.2024
Published Online07.06.2024

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