Aquatic Sciences and Engineering

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DOI :10.18864/TJAS201608   IUP :10.18864/TJAS201608    Full Text (PDF)

QUORUM SENSING SYSTEM AND VIRULENCE FACTORS IN Pseudomonas fluorescens FISH PATHOGEN

Nurdan Nurcan, Ayşegül Kubilay

In this study three Pseudomonas fluorescens strains, Gramnegative fish pathogen were used. Previously N-butanoyl-Lhomoserine lacton (BHL) and N-(3 okzododekanoyl)-L-homoserine lacton (OdDHL) signal molecules of production of strains were examined using biosensor strains, Choromobacterium violaceum CV026 strain and Agrobacterium tumefaciens NT1. Pseudomonas aeruginosa PAO1 strain used to as positive control. As a results of this study; P. fluorescens strains did not produce BHL signal molecules using C. violaceum CV026. However, the strains produced OdDHL signal molecules using A. tumafeciens NT1. It was determined production of biofilm, pigment, rhamnolipid, elastase, protease, amilase and hemolysis activity as phenotype tested as virulence factors in the strains

Keywords: P. fluorescens, Quorum sensing system, Virulence factors, Biofilm, Pigment, Rhamnolipid
DOI :10.18864/TJAS201608   IUP :10.18864/TJAS201608    Full Text (PDF)

Pseudomonas fluorescens BALIK PATOJENİNDE ÇEVREYİ ALGILAMA SİSTEMİ VE VİRÜLENS FAKTÖRLERİ

Nurdan Nurcan, Ayşegül Kubilay

Araştırmada Gram negatif bakteriyel balık patojenlerinden Pseudomonas fluorescens’in üç adet suşu kullanılmıştır. Suşlar da öncelikle N-butanoyl-L-homoserin lakton (BHL) ve N- (3-okzododekanoyl)-L-homoserin lakton (OdDHL) sinyal moleküllerinin üretimi, Chromobacterium violaceum CV026 ve Agrobacterium tumafeciens NT1 biyosensör suşları kullanılarak test edilmiştir. Pseudomonas aeruginosa PAO1 suşu pozitif kontrol olarak kullanılmıştır. Sonuç olarak, P. fluorescens suşları, C. violaceum CV026 suşu kullanıldığında BHL sinyal molekülünü üretmediği, bununla birlikte, A. tumafeciens NT1 kullanıldığında, P. fluorescens suşlarının OdDHL sinyal molekülünü ürettiği tespit edilmiştir. P. fluorescens suşları; biyofilm, pigment, ramnolipid, elastaz, amilaz, hemoliz ve proteaz aktiviteleri gibi virülens faktörlerini üretmiştir. 

Keywords: P. fluorescens, Çevreyi algılama sistemi, Virülens faktörleri, Biyofilm, Pigment, Ramnolipid

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APA

Nurcan, N., & Kubilay, A. (2016). QUORUM SENSING SYSTEM AND VIRULENCE FACTORS IN Pseudomonas fluorescens FISH PATHOGEN. Aquatic Sciences and Engineering, 31(2), 68-78. https://doi.org/10.18864/TJAS201608


AMA

Nurcan N, Kubilay A. QUORUM SENSING SYSTEM AND VIRULENCE FACTORS IN Pseudomonas fluorescens FISH PATHOGEN. Aquatic Sciences and Engineering. 2016;31(2):68-78. https://doi.org/10.18864/TJAS201608


ABNT

Nurcan, N.; Kubilay, A. QUORUM SENSING SYSTEM AND VIRULENCE FACTORS IN Pseudomonas fluorescens FISH PATHOGEN. Aquatic Sciences and Engineering, [Publisher Location], v. 31, n. 2, p. 68-78, 2016.


Chicago: Author-Date Style

Nurcan, Nurdan, and Ayşegül Kubilay. 2016. “QUORUM SENSING SYSTEM AND VIRULENCE FACTORS IN Pseudomonas fluorescens FISH PATHOGEN.” Aquatic Sciences and Engineering 31, no. 2: 68-78. https://doi.org/10.18864/TJAS201608


Chicago: Humanities Style

Nurcan, Nurdan, and Ayşegül Kubilay. “QUORUM SENSING SYSTEM AND VIRULENCE FACTORS IN Pseudomonas fluorescens FISH PATHOGEN.” Aquatic Sciences and Engineering 31, no. 2 (Jun. 2025): 68-78. https://doi.org/10.18864/TJAS201608


Harvard: Australian Style

Nurcan, N & Kubilay, A 2016, 'QUORUM SENSING SYSTEM AND VIRULENCE FACTORS IN Pseudomonas fluorescens FISH PATHOGEN', Aquatic Sciences and Engineering, vol. 31, no. 2, pp. 68-78, viewed 5 Jun. 2025, https://doi.org/10.18864/TJAS201608


Harvard: Author-Date Style

Nurcan, N. and Kubilay, A. (2016) ‘QUORUM SENSING SYSTEM AND VIRULENCE FACTORS IN Pseudomonas fluorescens FISH PATHOGEN’, Aquatic Sciences and Engineering, 31(2), pp. 68-78. https://doi.org/10.18864/TJAS201608 (5 Jun. 2025).


MLA

Nurcan, Nurdan, and Ayşegül Kubilay. “QUORUM SENSING SYSTEM AND VIRULENCE FACTORS IN Pseudomonas fluorescens FISH PATHOGEN.” Aquatic Sciences and Engineering, vol. 31, no. 2, 2016, pp. 68-78. [Database Container], https://doi.org/10.18864/TJAS201608


Vancouver

Nurcan N, Kubilay A. QUORUM SENSING SYSTEM AND VIRULENCE FACTORS IN Pseudomonas fluorescens FISH PATHOGEN. Aquatic Sciences and Engineering [Internet]. 5 Jun. 2025 [cited 5 Jun. 2025];31(2):68-78. Available from: https://doi.org/10.18864/TJAS201608 doi: 10.18864/TJAS201608


ISNAD

Nurcan, Nurdan - Kubilay, Ayşegül. “QUORUM SENSING SYSTEM AND VIRULENCE FACTORS IN Pseudomonas fluorescens FISH PATHOGEN”. Aquatic Sciences and Engineering 31/2 (Jun. 2025): 68-78. https://doi.org/10.18864/TJAS201608



TIMELINE


Accepted28.09.2016
Published Online04.12.2016

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