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DOI :10.26650/eor.20197456   IUP :10.26650/eor.20197456    Full Text (PDF)

Comparison of the effect of postoperative care agents on human gingival fibroblasts: a preliminary study

Uğur Mercan, Z.burçin Gönen, Hasan Salkin, Gül Merve Yalçın ülker, Deniz Gökçe Meral

Purpose 
The aim of this study is to compare effects of postoperative care agents; chlorhexidine, octenidine dihydrochloride and hyaluronic acid on human gingival fibroblasts’ viability, proliferation, apoptosis and migration. 

Material and Methods 
After cell culturing; chlorhexidine, octenidine dihydrochloride and hyaluronic acid solutions were applied on cells and nothing was applied for control group. The cells were monitored to investigate cytotoxicity; the percentage of apoptotic, living and dead cells at the time of 24, 48, and 72 hours (h). A scratch wound assay was performed to detect cell migration and cells were monitored at baseline, at 24 and 48h. 

Results 
At 24h, chlorhexidine showed statistically lower percentage of total apoptotic cells’ than octenidine dihydrochloride (p=0.049), hyaluronic acid (p=0.049) and control (p=0.049). At 48h, hyaluronic acid showed statistically lower percentage than chlorhexidine (p=0.049), and control (p=0.049). All agents were found to have statistically and significantly more cytotoxic than control. However, there was no difference between experimental groups for proliferation rate. Octenidine dihydrochloride showed statistically negative effects on cell migration than chlorhexidine and hyaluronic acid at 24h. Chlorhexidine and hyaluronic acid maintained migration ability of cells than octenidine dihydrochloride at 48h. 

Conclusion 
All agents have similar effects on cell behavior such as viability, apoptosis and cell proliferation. However, octenidine dihydrochloride showed statistically negative effects on migration ability than chlorhexidine and hyaluronic acid. 
Keywords: Chlorhexidine, hyaluronic acid, octenidine dihydrochloride, cell viability, cell migration

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APA

Mercan, U., Gönen, Z., Salkin, H., Yalçın ülker, G.M., & Meral, D.G. (2019). Comparison of the effect of postoperative care agents on human gingival fibroblasts: a preliminary study. European Oral Research, 53(2), 67-73. https://doi.org/10.26650/eor.20197456


AMA

Mercan U, Gönen Z, Salkin H, Yalçın ülker G M, Meral D G. Comparison of the effect of postoperative care agents on human gingival fibroblasts: a preliminary study. European Oral Research. 2019;53(2):67-73. https://doi.org/10.26650/eor.20197456


ABNT

Mercan, U.; Gönen, Z.; Salkin, H.; Yalçın ülker, G.M.; Meral, D.G. Comparison of the effect of postoperative care agents on human gingival fibroblasts: a preliminary study. European Oral Research, [Publisher Location], v. 53, n. 2, p. 67-73, 2019.


Chicago: Author-Date Style

Mercan, Uğur, and Z.burçin Gönen and Hasan Salkin and Gül Merve Yalçın ülker and Deniz Gökçe Meral. 2019. “Comparison of the effect of postoperative care agents on human gingival fibroblasts: a preliminary study.” European Oral Research 53, no. 2: 67-73. https://doi.org/10.26650/eor.20197456


Chicago: Humanities Style

Mercan, Uğur, and Z.burçin Gönen and Hasan Salkin and Gül Merve Yalçın ülker and Deniz Gökçe Meral. “Comparison of the effect of postoperative care agents on human gingival fibroblasts: a preliminary study.” European Oral Research 53, no. 2 (Sep. 2024): 67-73. https://doi.org/10.26650/eor.20197456


Harvard: Australian Style

Mercan, U & Gönen, Z & Salkin, H & Yalçın ülker, GM & Meral, DG 2019, 'Comparison of the effect of postoperative care agents on human gingival fibroblasts: a preliminary study', European Oral Research, vol. 53, no. 2, pp. 67-73, viewed 16 Sep. 2024, https://doi.org/10.26650/eor.20197456


Harvard: Author-Date Style

Mercan, U. and Gönen, Z. and Salkin, H. and Yalçın ülker, G.M. and Meral, D.G. (2019) ‘Comparison of the effect of postoperative care agents on human gingival fibroblasts: a preliminary study’, European Oral Research, 53(2), pp. 67-73. https://doi.org/10.26650/eor.20197456 (16 Sep. 2024).


MLA

Mercan, Uğur, and Z.burçin Gönen and Hasan Salkin and Gül Merve Yalçın ülker and Deniz Gökçe Meral. “Comparison of the effect of postoperative care agents on human gingival fibroblasts: a preliminary study.” European Oral Research, vol. 53, no. 2, 2019, pp. 67-73. [Database Container], https://doi.org/10.26650/eor.20197456


Vancouver

Mercan U, Gönen Z, Salkin H, Yalçın ülker GM, Meral DG. Comparison of the effect of postoperative care agents on human gingival fibroblasts: a preliminary study. European Oral Research [Internet]. 16 Sep. 2024 [cited 16 Sep. 2024];53(2):67-73. Available from: https://doi.org/10.26650/eor.20197456 doi: 10.26650/eor.20197456


ISNAD

Mercan, Uğur - Gönen, Z.burçin - Salkin, Hasan - Yalçın ülker, GülMerve - Meral, DenizGökçe. “Comparison of the effect of postoperative care agents on human gingival fibroblasts: a preliminary study”. European Oral Research 53/2 (Sep. 2024): 67-73. https://doi.org/10.26650/eor.20197456



TIMELINE


Submitted16.01.2018
Accepted25.10.2018
Published Online27.05.2019

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