Research Article

DOI :10.26650/eor.20241449626 IUP :10.26650/eor.20241449626 Full Text (PDF)

The influence of toluidine blue staining on decision-making for the selection of biopsy sites in oral disorders

Ceyda Gürhan, Betül İlhan, Barış Oğuz Gürses, Gaye Bölükbaşı, Pelin Güneri

Purpose: The aim of this study is to evaluate the influence of toluidine blue (TB) staining on decision-making processes for the identification of biopsy sites in oral lesions.

Material-Methods: Original and TB-stained images of oral mucosal lesions that required histopathological examination were accessed through archives. Twenty dental specialists were asked to select and mark their choice of biopsy sites on both original and TB-stained images using a standard circle selection tool of software. The X and Y coordinates of the center points of selections were calculated by a custom-made image processing algorithm in Matlab. In order to assess the dispersity of the selected centers from the mean center, the distances of the selected points from this average point were calculated using Euclidean distance. Data was analysed using Wilcoxon test.

Results: The study involved original and TB-stained images of 5 suspicious lesions. The histological diagnoses of oral lesions were reported as bening for 3 cases and malignant for 2 cases. 20 dental specialists marked their choice of biopsy site on a total of 200 images. The results revealed that the biopsy selections varied significantly between original and TB-stained images in certain cases. In 60% of cases, specialists showed statistically significant agreement in biopsy site selection on TB-stained images compared to original images (p <0.05)

Conclusion: While the study did not evaluate the accuracy of site selection against a gold standard, the observed consensus among specialists suggests the potential utility of TB staining in enhancing the consistency and objectivity of biopsy site selection for suspicious oral lesions.

Keywords: oral mucosal lesion, tissue biopsy, biopsy site selection, vital staining, toluidine blue

DOI :10.26650/eor.20241449626 IUP :10.26650/eor.20241449626 Full Text (PDF)

Ağız Hastalıklarında Toluidin Mavisi ile Boyamanın Biyopsi Yeri Seçimine Karar Verme Sürecindeki Etkisinin Değerlendirilmesi

Ceyda Gürhan, Betül İlhan, Barış Oğuz Gürses, Gaye Bölükbaşı, Pelin Güneri

Amaç: Bu çalışmanın amacı Toluidin mavisi (TM) ile boyamanın, oral lezyonlarda biyopsi yeri seçimine karar verme sürecindeki etkisini değerlendirmek.

Gereç ve Yöntem: Çalışmaya histopatolojik inceleme gerektiren oral mukozal lezyonların orijinal ve TM ile boyalı arşiv görüntüleri dahil edildi. 20 adet uzman diş hekiminden biyopsi alanı olarak belirledikleri bölgeleri, yazılımın standart yuvarlak seçim aracını kullanarak hem orijinal hem de TM ile boyalı görüntüler üzerinde işaretlemeleri istendi. Uzmanlar tarafından belirlenen biyopsi alanlarının merkez noktalarına ait X ve Y koordinatları Matlab'da özel bir görüntü işleme algoritması ile hesaplandı. Seçilen merkezlerin ortalama merkez etrafındaki dağılımları ve ortalama merkeze olan uzaklıkları Öklid mesafesi kullanılarak belirlendi. Veriler Wilcoxon testi kullanılarak analiz edildi.

Bulgular: Çalışmaya 5 adet şüpheli oral lezyona ait orjinal ve TM ile boyanmış görüntüler dahil edildi. Bu lezyonların 3’ü histopatolojik olarak benign, 2 olgu ise malign olarak rapor edildi. 20 adet uzman diş hekimi toplam 200 adet görüntü üzerinde biyopsi alanı olarak belirledikleri alanları işaratledi. Sonuçlar bazı olgularda orijinal ve TB boyalı görüntüler arasında biyopsi alanı seçimi önemli ölçüde farklılık olduğunu ortaya koydu. Olguların %60'ında, uzmanlar biyopsi yeri seçiminde TM ile boyalı görüntülerde orijinal görüntülere kıyasla istatistiksel olarak anlamlı şekilde fikir birliği sergilediler (p<0.05).

Sonuç: Bu çalışmada biyopsi yeri seçiminin doğruluğu bir altın standartla karşılaştırılmamış olmakla birlikte, TM ile boyanan lezyonlarda uzmanlar arasında biyopsi alanı seçimine yönelik gözlenen konsensus, şüpheli oral lezyonlarda TM ile boyamanın biyopsi yeri seçiminde tutarlılığı ve objektifliği arttırmadaki potansiyel katkısını ortaya koymaktadır.

Keywords: oral mukozal lezyon, doku biyopsisi, biyopsi yeri seçimi, vital boyama, toluidin mavisi

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APA

Gürhan, C., İlhan, B., Gürses, B.O., Bölükbaşı, G., & Güneri, P. (2024). The influence of toluidine blue staining on decision-making for the selection of biopsy sites in oral disorders. European Oral Research, 0(0), -. https://doi.org/10.26650/eor.20241449626

AMA

Gürhan C, İlhan B, Gürses B O, Bölükbaşı G, Güneri P. The influence of toluidine blue staining on decision-making for the selection of biopsy sites in oral disorders. European Oral Research. 2024;0(0):-. https://doi.org/10.26650/eor.20241449626

ABNT

Gürhan, C.; İlhan, B.; Gürses, B.O.; Bölükbaşı, G.; Güneri, P. The influence of toluidine blue staining on decision-making for the selection of biopsy sites in oral disorders. European Oral Research, [Publisher Location], v. 0, n. 0, p. -, 2024.

Chicago: Author-Date Style

Gürhan, Ceyda, and Betül İlhan and Barış Oğuz Gürses and Gaye Bölükbaşı and Pelin Güneri. 2024. “The influence of toluidine blue staining on decision-making for the selection of biopsy sites in oral disorders.” European Oral Research 0, no. 0: -. https://doi.org/10.26650/eor.20241449626

Chicago: Humanities Style

Gürhan, Ceyda, and Betül İlhan and Barış Oğuz Gürses and Gaye Bölükbaşı and Pelin Güneri. “The influence of toluidine blue staining on decision-making for the selection of biopsy sites in oral disorders.” European Oral Research 0, no. 0 (Dec. 2024): -. https://doi.org/10.26650/eor.20241449626

Harvard: Australian Style

Gürhan, C & İlhan, B & Gürses, BO & Bölükbaşı, G & Güneri, P 2024, 'The influence of toluidine blue staining on decision-making for the selection of biopsy sites in oral disorders', European Oral Research, vol. 0, no. 0, pp. -, viewed 23 Dec. 2024, https://doi.org/10.26650/eor.20241449626

Harvard: Author-Date Style

Gürhan, C. and İlhan, B. and Gürses, B.O. and Bölükbaşı, G. and Güneri, P. (2024) ‘The influence of toluidine blue staining on decision-making for the selection of biopsy sites in oral disorders’, European Oral Research, 0(0), pp. -. https://doi.org/10.26650/eor.20241449626 (23 Dec. 2024).

MLA

Gürhan, Ceyda, and Betül İlhan and Barış Oğuz Gürses and Gaye Bölükbaşı and Pelin Güneri. “The influence of toluidine blue staining on decision-making for the selection of biopsy sites in oral disorders.” European Oral Research, vol. 0, no. 0, 2024, pp. -. [Database Container], https://doi.org/10.26650/eor.20241449626

Vancouver

Gürhan C, İlhan B, Gürses BO, Bölükbaşı G, Güneri P. The influence of toluidine blue staining on decision-making for the selection of biopsy sites in oral disorders. European Oral Research [Internet]. 23 Dec. 2024 [cited 23 Dec. 2024];0(0):-. Available from: https://doi.org/10.26650/eor.20241449626 doi: 10.26650/eor.20241449626

ISNAD

Gürhan, Ceyda - İlhan, Betül - Gürses, BarışOğuz - Bölükbaşı, Gaye - Güneri, Pelin. “The influence of toluidine blue staining on decision-making for the selection of biopsy sites in oral disorders”. European Oral Research 0/0 (Dec. 2024): -. https://doi.org/10.26650/eor.20241449626

TIMELINE

Submitted	09.03.2024
Accepted	16.05.2024
Published Online	16.07.2024

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