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DOI :10.26650/experimed.2020.0008   IUP :10.26650/experimed.2020.0008    Full Text (PDF)

Investigation of IL-10 Gene Expression and rs1554286 SNP in Behçet's Disease

Elçin Şehitoğlu Taşar, Elif Uğurel, Erdem Tüzün, Burçak Vural

Objective: Behçet's disease (BD) is a chronic, recurrent and inflammatory disorder characterized by oral and genital aphthous ulcerations, uveitis, skin lesions and skin pathergy reaction. There are many genetic studies focused on determining BD etiology. In this study, we aimed to investigate IL-10 gene expression and rs1554286 single nucleotide polymorphism (SNP) which is located in IL-10 gene. Material and Method: In this study, the blood specimens of BD patients (n=45) and healthy controls (HC) (n=28) were collected for RNA and DNA extraction. We genotyped rs1554286 from the DNA samples (45 BD, 28 HC) using real time PCR. We identified the IL-10 gene expression levels from cDNA samples of patients with sufficient sample amounts (29 patients with BD and 23 HCs). Results: We compared the expression levels of IL-10 gene and also genotyping analysis, and no significant difference was observed between BD patients and HC. Conclusion: In our study, which compared IL-10 gene expression and SNP genotyping between Behçet patients and healthy controls, no difference was found between the two groups. Since this may have occurred due to the low number of samples in our study, studies with higher sample numbers are needed.  

Keywords: Behçet's disease, IL-10, rs1554286
DOI :10.26650/experimed.2020.0008   IUP :10.26650/experimed.2020.0008    Full Text (PDF)

Behçet Hastalığı’nda IL-10 Geni Ekspresyonu ve rs1554286 SNP İncelenmesi

Elçin Şehitoğlu Taşar, Elif Uğurel, Erdem Tüzün, Burçak Vural

Amaç: Behçet hastalığı (BH) genel olarak tekrarlayan oral aftlar, genital ülserasyonlar, deri lezyonları ve üveit ile karakterize olan kronik inflamatuar bir hastalıktır. BH’nın etiyopatogenezini aydınlatmayı hedefleyen pek çok genetik çalışma bulunmaktadır. Bu çalışmada IL-10’da bulunan rs1554286 tek nukleotid polimorfizmi (SNP) ve IL10 gen ekspresyonunun BH’ında incelenmesi amaçlanmıştır. Gereç ve Yöntem: Bu çalışmada DNA ve RNA izolasyonu için Behçet Hastaları (n=45) ve sağlıklı kontrollere (n=28) ait kan örnekleri toplanmış ve rs1554286 SNP genotiplemesi gerçek zamanlı PZR ile çalışılmıştır. Yeterli miktarda RNA elde edilen örneklerden (29 BH, 23 sağlıklı kontrol) cDNA elde edilerek IL-10 gen ekspresyonu yapılmıştır. Bulgular: IL-10 geni ekspresyon ve SNP genotiplemesi sonucunda BH ve sağlıklı kontroller arasında istatistiksel olarak anlamlı bir sonuç bulunmamıştır. Sonuç: Behçet hastaları ve sağlıklı kontroller arasında IL-10 geni ekspresyon ve SNP genotiplemesi karşılaştırması yapılan çalışmamızda iki grup arasında fark saptanmamıştır. Bu durum çalışmamızdaki örneklem sayısının azlığı nedeniyle ortaya çıkmış olabileceğinden daha yüksek örneklem sayılı çalışmalara ihtiyaç duyulmaktadır.

Keywords: Behçet hastalığı, IL-10, rs1554286

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References

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APA

Şehitoğlu Taşar, E., Uğurel, E., Tüzün, E., & Vural, B. (2020). Investigation of IL-10 Gene Expression and rs1554286 SNP in Behçet's Disease. Experimed, 10(1), 25-29. https://doi.org/10.26650/experimed.2020.0008


AMA

Şehitoğlu Taşar E, Uğurel E, Tüzün E, Vural B. Investigation of IL-10 Gene Expression and rs1554286 SNP in Behçet's Disease. Experimed. 2020;10(1):25-29. https://doi.org/10.26650/experimed.2020.0008


ABNT

Şehitoğlu Taşar, E.; Uğurel, E.; Tüzün, E.; Vural, B. Investigation of IL-10 Gene Expression and rs1554286 SNP in Behçet's Disease. Experimed, [Publisher Location], v. 10, n. 1, p. 25-29, 2020.


Chicago: Author-Date Style

Şehitoğlu Taşar, Elçin, and Elif Uğurel and Erdem Tüzün and Burçak Vural. 2020. “Investigation of IL-10 Gene Expression and rs1554286 SNP in Behçet's Disease.” Experimed 10, no. 1: 25-29. https://doi.org/10.26650/experimed.2020.0008


Chicago: Humanities Style

Şehitoğlu Taşar, Elçin, and Elif Uğurel and Erdem Tüzün and Burçak Vural. “Investigation of IL-10 Gene Expression and rs1554286 SNP in Behçet's Disease.” Experimed 10, no. 1 (Dec. 2024): 25-29. https://doi.org/10.26650/experimed.2020.0008


Harvard: Australian Style

Şehitoğlu Taşar, E & Uğurel, E & Tüzün, E & Vural, B 2020, 'Investigation of IL-10 Gene Expression and rs1554286 SNP in Behçet's Disease', Experimed, vol. 10, no. 1, pp. 25-29, viewed 21 Dec. 2024, https://doi.org/10.26650/experimed.2020.0008


Harvard: Author-Date Style

Şehitoğlu Taşar, E. and Uğurel, E. and Tüzün, E. and Vural, B. (2020) ‘Investigation of IL-10 Gene Expression and rs1554286 SNP in Behçet's Disease’, Experimed, 10(1), pp. 25-29. https://doi.org/10.26650/experimed.2020.0008 (21 Dec. 2024).


MLA

Şehitoğlu Taşar, Elçin, and Elif Uğurel and Erdem Tüzün and Burçak Vural. “Investigation of IL-10 Gene Expression and rs1554286 SNP in Behçet's Disease.” Experimed, vol. 10, no. 1, 2020, pp. 25-29. [Database Container], https://doi.org/10.26650/experimed.2020.0008


Vancouver

Şehitoğlu Taşar E, Uğurel E, Tüzün E, Vural B. Investigation of IL-10 Gene Expression and rs1554286 SNP in Behçet's Disease. Experimed [Internet]. 21 Dec. 2024 [cited 21 Dec. 2024];10(1):25-29. Available from: https://doi.org/10.26650/experimed.2020.0008 doi: 10.26650/experimed.2020.0008


ISNAD

Şehitoğlu Taşar, Elçin - Uğurel, Elif - Tüzün, Erdem - Vural, Burçak. “Investigation of IL-10 Gene Expression and rs1554286 SNP in Behçet's Disease”. Experimed 10/1 (Dec. 2024): 25-29. https://doi.org/10.26650/experimed.2020.0008



TIMELINE


Submitted12.03.2020
Accepted27.03.2020
Published Online13.04.2020

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