Experimed

SUBMITINFORMATION
EnglishTürkçe

Original Research


DOI :10.26650/experimed.2020.0008   IUP :10.26650/experimed.2020.0008    Full Text (PDF)

Investigation of IL-10 Gene Expression and rs1554286 SNP in Behçet's Disease

Elçin Şehitoğlu Taşar, Elif Uğurel, Erdem Tüzün, Burçak Vural

Objective: Behçet's disease (BD) is a chronic, recurrent and inflammatory disorder characterized by oral and genital aphthous ulcerations, uveitis, skin lesions and skin pathergy reaction. There are many genetic studies focused on determining BD etiology. In this study, we aimed to investigate IL-10 gene expression and rs1554286 single nucleotide polymorphism (SNP) which is located in IL-10 gene. Material and Method: In this study, the blood specimens of BD patients (n=45) and healthy controls (HC) (n=28) were collected for RNA and DNA extraction. We genotyped rs1554286 from the DNA samples (45 BD, 28 HC) using real time PCR. We identified the IL-10 gene expression levels from cDNA samples of patients with sufficient sample amounts (29 patients with BD and 23 HCs). Results: We compared the expression levels of IL-10 gene and also genotyping analysis, and no significant difference was observed between BD patients and HC. Conclusion: In our study, which compared IL-10 gene expression and SNP genotyping between Behçet patients and healthy controls, no difference was found between the two groups. Since this may have occurred due to the low number of samples in our study, studies with higher sample numbers are needed.  

Keywords: Behçet's disease, IL-10, rs1554286
DOI :10.26650/experimed.2020.0008   IUP :10.26650/experimed.2020.0008    Full Text (PDF)

Behçet Hastalığı’nda IL-10 Geni Ekspresyonu ve rs1554286 SNP İncelenmesi

Elçin Şehitoğlu Taşar, Elif Uğurel, Erdem Tüzün, Burçak Vural

Amaç: Behçet hastalığı (BH) genel olarak tekrarlayan oral aftlar, genital ülserasyonlar, deri lezyonları ve üveit ile karakterize olan kronik inflamatuar bir hastalıktır. BH’nın etiyopatogenezini aydınlatmayı hedefleyen pek çok genetik çalışma bulunmaktadır. Bu çalışmada IL-10’da bulunan rs1554286 tek nukleotid polimorfizmi (SNP) ve IL10 gen ekspresyonunun BH’ında incelenmesi amaçlanmıştır. Gereç ve Yöntem: Bu çalışmada DNA ve RNA izolasyonu için Behçet Hastaları (n=45) ve sağlıklı kontrollere (n=28) ait kan örnekleri toplanmış ve rs1554286 SNP genotiplemesi gerçek zamanlı PZR ile çalışılmıştır. Yeterli miktarda RNA elde edilen örneklerden (29 BH, 23 sağlıklı kontrol) cDNA elde edilerek IL-10 gen ekspresyonu yapılmıştır. Bulgular: IL-10 geni ekspresyon ve SNP genotiplemesi sonucunda BH ve sağlıklı kontroller arasında istatistiksel olarak anlamlı bir sonuç bulunmamıştır. Sonuç: Behçet hastaları ve sağlıklı kontroller arasında IL-10 geni ekspresyon ve SNP genotiplemesi karşılaştırması yapılan çalışmamızda iki grup arasında fark saptanmamıştır. Bu durum çalışmamızdaki örneklem sayısının azlığı nedeniyle ortaya çıkmış olabileceğinden daha yüksek örneklem sayılı çalışmalara ihtiyaç duyulmaktadır.

Keywords: Behçet hastalığı, IL-10, rs1554286

PDF View

References

  • 1. Alpsoy E, Aktekin M, Er H, Durusoy C, Yilmaz E. Distribution and frequency of papulopustular lesions in Behçet's disease. Int J Dermatol 1998; 37: 839-43. [CrossRef] google scholar
  • 2. Borlu M. Behçet Hastalığında Etyopatogenez ve Klinik bulgular. Sağlık Bilimleri Dergisi (Journal of Health Sciences) 2007; 16(1): 63-72. google scholar
  • 3. Gül A, Ollier WE, Silman AJ, Worthington J. Behçet's disease: An update on the pathogenesis. Clin Exp Rheumatol. 2001; 19(Suppl. 24): S6-S12. google scholar
  • 4. Karasneh J, Gül A, Ollier WE, Silman AJ, Worthington J. Whole-Genome Screening for Susceptibility Genes in Multicase Families With Behcet's Disease. Arthritis Rheum 2005; 52: 1836-42. [CrossRef] google scholar
  • 5. Mizuki N, Meguro A, Ota M, Ohno S, Shiota T, Kawagoe T, et al., Genome-wide association studies identify IL23R-IL12RB2 and IL10 as Behçet's disease susceptibility loci. Nature Genetetic 2010; 42: 703-7. [CrossRef] google scholar
  • 6. Remmers EF, Cosan F, Kirino Y, Ombrello MJ, Abaci N, Satorius C, Le JM, et al., Genome-wide association study identifies variants in the MHC Class 1, IL 10 and IL 23R-IL12RB2 regions associated with Behcet's disease. Nature Genetic 2010; 42: 698-702. [CrossRef] google scholar
  • 7. Hou S, Yang Z, Du L, Jiang Z, Shu Q, Chen Y, et al. Identification of a susceptibility locus in STAT4 for Behçet's disease in Han Chinese in agenome-wide association study. Arthritis Rheum 2012; 64: 410413. [CrossRef] google scholar
  • 8. Kang EH, Kim S, Young Park M, Choi JY, Choi IA, Kim MJ, et al. Behçet's disease risk association fine-mapped on the IL23R-IL12RB2 intergenic region in Koreans. Arthritis Res Ther 2017; 19: 227. [CrossRef] google scholar
  • 9. Afkari B, Babaloo Z, Dolati S, Khabazi A, Jadidi-Niaragh F, Talei M, et al. Molecular analysis of interleukin-10 gene polymorphisms in patients with Behçet's disease. Immunol Lett 2018; 194: 56-61. [CrossRef] google scholar
  • 10. Alipour S, Nouri M, Khabbazi A, Samadi N, Babaloo Z, Abolhasani S, et al., Hypermethylation of IL-10 gene is responsible for its low mRNA expression in Behçet's disease. J Cell Biochem 2018; 119: 6614-22. [CrossRef] google scholar
  • 11. Yu H, Zheng M, Zhang L, Li H, Zhu Y, Cheng L, Li L, et al. Identification of susceptibility SNPs in IL10 and IL23R-IL12RB2 for Behçet's disease in Han Chinese. J Allergy Clin Immunol 2017; 139: 621-7. [CrossRef] google scholar

Citations

Copy and paste a formatted citation or use one of the options to export in your chosen format


EXPORT



APA

Şehitoğlu Taşar, E., Uğurel, E., Tüzün, E., & Vural, B. (2020). Investigation of IL-10 Gene Expression and rs1554286 SNP in Behçet's Disease. Experimed, 10(1), 25-29. https://doi.org/10.26650/experimed.2020.0008


AMA

Şehitoğlu Taşar E, Uğurel E, Tüzün E, Vural B. Investigation of IL-10 Gene Expression and rs1554286 SNP in Behçet's Disease. Experimed. 2020;10(1):25-29. https://doi.org/10.26650/experimed.2020.0008


ABNT

Şehitoğlu Taşar, E.; Uğurel, E.; Tüzün, E.; Vural, B. Investigation of IL-10 Gene Expression and rs1554286 SNP in Behçet's Disease. Experimed, [Publisher Location], v. 10, n. 1, p. 25-29, 2020.


Chicago: Author-Date Style

Şehitoğlu Taşar, Elçin, and Elif Uğurel and Erdem Tüzün and Burçak Vural. 2020. “Investigation of IL-10 Gene Expression and rs1554286 SNP in Behçet's Disease.” Experimed 10, no. 1: 25-29. https://doi.org/10.26650/experimed.2020.0008


Chicago: Humanities Style

Şehitoğlu Taşar, Elçin, and Elif Uğurel and Erdem Tüzün and Burçak Vural. “Investigation of IL-10 Gene Expression and rs1554286 SNP in Behçet's Disease.” Experimed 10, no. 1 (May. 2024): 25-29. https://doi.org/10.26650/experimed.2020.0008


Harvard: Australian Style

Şehitoğlu Taşar, E & Uğurel, E & Tüzün, E & Vural, B 2020, 'Investigation of IL-10 Gene Expression and rs1554286 SNP in Behçet's Disease', Experimed, vol. 10, no. 1, pp. 25-29, viewed 11 May. 2024, https://doi.org/10.26650/experimed.2020.0008


Harvard: Author-Date Style

Şehitoğlu Taşar, E. and Uğurel, E. and Tüzün, E. and Vural, B. (2020) ‘Investigation of IL-10 Gene Expression and rs1554286 SNP in Behçet's Disease’, Experimed, 10(1), pp. 25-29. https://doi.org/10.26650/experimed.2020.0008 (11 May. 2024).


MLA

Şehitoğlu Taşar, Elçin, and Elif Uğurel and Erdem Tüzün and Burçak Vural. “Investigation of IL-10 Gene Expression and rs1554286 SNP in Behçet's Disease.” Experimed, vol. 10, no. 1, 2020, pp. 25-29. [Database Container], https://doi.org/10.26650/experimed.2020.0008


Vancouver

Şehitoğlu Taşar E, Uğurel E, Tüzün E, Vural B. Investigation of IL-10 Gene Expression and rs1554286 SNP in Behçet's Disease. Experimed [Internet]. 11 May. 2024 [cited 11 May. 2024];10(1):25-29. Available from: https://doi.org/10.26650/experimed.2020.0008 doi: 10.26650/experimed.2020.0008


ISNAD

Şehitoğlu Taşar, Elçin - Uğurel, Elif - Tüzün, Erdem - Vural, Burçak. “Investigation of IL-10 Gene Expression and rs1554286 SNP in Behçet's Disease”. Experimed 10/1 (May. 2024): 25-29. https://doi.org/10.26650/experimed.2020.0008



TIMELINE


Submitted12.03.2020
Accepted27.03.2020
Published Online13.04.2020

LICENCE


Attribution-NonCommercial (CC BY-NC)

This license lets others remix, tweak, and build upon your work non-commercially, and although their new works must also acknowledge you and be non-commercial, they don’t have to license their derivative works on the same terms.


SHARE




Istanbul University Press aims to contribute to the dissemination of ever growing scientific knowledge through publication of high quality scientific journals and books in accordance with the international publishing standards and ethics. Istanbul University Press follows an open access, non-commercial, scholarly publishing.