Biological and Immunological Properties of Mesenchymal Stromal  Cells Derived From Bone Marrow in Childhood Acute Myeloid  Leukemia

Pişkin, İlkay; Özdemir Sancı, Tuba; Köksal, Yasin; Yaralı, H. Neşe; Karaca Kara, Fatma; Tunç, Bahattin; Özgüner, Meltem

doi:https://dx.doi.org/10.26650/experimed.1558871

Research Article

DOI :10.26650/experimed.1558871 IUP :10.26650/experimed.1558871 Full Text (PDF)

Biological and Immunological Properties of Mesenchymal Stromal Cells Derived From Bone Marrow in Childhood Acute Myeloid Leukemia

İlkay Pişkin, Tuba Özdemir Sancı, Yasin Köksal, H. Neşe Yaralı, Fatma Karaca Kara, Bahattin Tunç, Meltem Özgüner

Objective: Pediatric acute myeloid leukemia (AML) is a common form of pediatric leukemia and is characterized by the accumulation of abnormal white blood cells, called blasts, in the bone marrow (BM). The aim of this study was to understand the BM microenvironment by studying the biological and immunological properties of BM-derived mesenchymal stromal cells (MSCs) and mononuclear cells (MNCs) to elucidate the potential role of phytohaemag glutinin in cell viability.

Materials and Methods: BM and peripheral blood samples were obtained from seven pediatric AML patients and seven donors. BM-MSCs and MNCs were isolated and characterized. Population doubling (PD) values, adipogenic and osteogenic differentiation capacity, cell viability, phytohemagglutinin (PHA) assay, and flow cytometry were performed.

Results: Mononucleated cells from peripheral blood of AML patients and donors and T-cell activation markers (CD3+CD69+, CD4+C25+, CD3+HLA-DR+) were measured by flow cytometry (𝜒2=2.184; p=0.823). BM-MSCs were co-cultured with MNCs, and PD values for AML patients were similar to those of donors (z=1.074; p=0.394). It was statistically significant when healthy MNC and healthy MNC PHA(+) groups were compared (p=0.015). When healthy MNC PHA(+) and healthy MSC+AML MNC PHA(+) groups were compared, it was found to be statistically significant (p=0.014).

Conclusion: This interaction is also not unidirectional. This interaction serves as a marker for understanding the immunological effects of AML.

Keywords: Pediatric AML, BM-MSCs, PHA, Cell viability, MNCs

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APA

Pişkin, İ., Özdemir Sancı, T., Köksal, Y., Yaralı, H., Karaca Kara, F., Tunç, B., & Özgüner, M. (2025). Biological and Immunological Properties of Mesenchymal Stromal Cells Derived From Bone Marrow in Childhood Acute Myeloid Leukemia. Experimed, 15(1), 66-76. https://doi.org/10.26650/experimed.1558871

AMA

Pişkin İ, Özdemir Sancı T, Köksal Y, Yaralı H, Karaca Kara F, Tunç B, Özgüner M. Biological and Immunological Properties of Mesenchymal Stromal Cells Derived From Bone Marrow in Childhood Acute Myeloid Leukemia. Experimed. 2025;15(1):66-76. https://doi.org/10.26650/experimed.1558871

ABNT

Pişkin, İ.; Özdemir Sancı, T.; Köksal, Y.; Yaralı, H.; Karaca Kara, F.; Tunç, B.; Özgüner, M. Biological and Immunological Properties of Mesenchymal Stromal Cells Derived From Bone Marrow in Childhood Acute Myeloid Leukemia. Experimed, [Publisher Location], v. 15, n. 1, p. 66-76, 2025.

Chicago: Author-Date Style

Pişkin, İlkay, and Tuba Özdemir Sancı and Yasin Köksal and H. Neşe Yaralı and Fatma Karaca Kara and Bahattin Tunç and Meltem Özgüner. 2025. “Biological and Immunological Properties of Mesenchymal Stromal Cells Derived From Bone Marrow in Childhood Acute Myeloid Leukemia.” Experimed 15, no. 1: 66-76. https://doi.org/10.26650/experimed.1558871

Chicago: Humanities Style

Pişkin, İlkay, and Tuba Özdemir Sancı and Yasin Köksal and H. Neşe Yaralı and Fatma Karaca Kara and Bahattin Tunç and Meltem Özgüner. “Biological and Immunological Properties of Mesenchymal Stromal Cells Derived From Bone Marrow in Childhood Acute Myeloid Leukemia.” Experimed 15, no. 1 (May. 2025): 66-76. https://doi.org/10.26650/experimed.1558871

Harvard: Australian Style

Pişkin, İ & Özdemir Sancı, T & Köksal, Y & Yaralı, H & Karaca Kara, F & Tunç, B & Özgüner, M 2025, 'Biological and Immunological Properties of Mesenchymal Stromal Cells Derived From Bone Marrow in Childhood Acute Myeloid Leukemia', Experimed, vol. 15, no. 1, pp. 66-76, viewed 22 May. 2025, https://doi.org/10.26650/experimed.1558871

Harvard: Author-Date Style

Pişkin, İ. and Özdemir Sancı, T. and Köksal, Y. and Yaralı, H. and Karaca Kara, F. and Tunç, B. and Özgüner, M. (2025) ‘Biological and Immunological Properties of Mesenchymal Stromal Cells Derived From Bone Marrow in Childhood Acute Myeloid Leukemia’, Experimed, 15(1), pp. 66-76. https://doi.org/10.26650/experimed.1558871 (22 May. 2025).

MLA

Pişkin, İlkay, and Tuba Özdemir Sancı and Yasin Köksal and H. Neşe Yaralı and Fatma Karaca Kara and Bahattin Tunç and Meltem Özgüner. “Biological and Immunological Properties of Mesenchymal Stromal Cells Derived From Bone Marrow in Childhood Acute Myeloid Leukemia.” Experimed, vol. 15, no. 1, 2025, pp. 66-76. [Database Container], https://doi.org/10.26650/experimed.1558871

Vancouver

Pişkin İ, Özdemir Sancı T, Köksal Y, Yaralı H, Karaca Kara F, Tunç B, Özgüner M. Biological and Immunological Properties of Mesenchymal Stromal Cells Derived From Bone Marrow in Childhood Acute Myeloid Leukemia. Experimed [Internet]. 22 May. 2025 [cited 22 May. 2025];15(1):66-76. Available from: https://doi.org/10.26650/experimed.1558871 doi: 10.26650/experimed.1558871

ISNAD

Pişkin, İlkay - Özdemir Sancı, Tuba - Köksal, Yasin - Yaralı, H. Neşe - Karaca Kara, Fatma - Tunç, Bahattin - Özgüner, Meltem. “Biological and Immunological Properties of Mesenchymal Stromal Cells Derived From Bone Marrow in Childhood Acute Myeloid Leukemia”. Experimed 15/1 (May. 2025): 66-76. https://doi.org/10.26650/experimed.1558871

Volume 15, Issue 12025, P. 66-76

TIMELINE

Submitted	30.09.2024
Accepted	21.02.2025
Published Online	28.03.2025

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