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DOI :10.26650/experimed.1408284   IUP :10.26650/experimed.1408284    Full Text (PDF)

Cytogenetic and FISH Examination of 3p Abnormalities in Lung Cancer Patients

Narmin Bakhshaliyeva, Ayşe Çırakoğlu, Hürrem Gül Öngen, Esin Bil Tuncay, Yelda Tarkan Argüden

Objective: Deletions or loss of heterozygosity in chromosome 3p are very common in small-cell lung cancer (SCLC) and lung adenocarcinoma (ADC) cases. These are typically found in tumor cells but rarely observed in lymphocytes. This study aimed to evaluate the frequency of 3p deletions and/or abnormalities in the blood of lung cancer patients using conventional cytogenetics and fluorescence in situ hybridization (FISH), by targeting the fragile histidine triad diadenosine triphosphatase (FHIT) gene located at the commonly deleted region of 3p14.2, in lung cancers.

Materials and Methods: The study examined 24 SCLC patients, 30 ADC patients, and 20 healthy controls. It used standard procedures to perform a 72-h lymphocyte culture, G-banding, and FISH.

Results: All patient group cases showed multiple numerical and structural abnormalities, with numerical abnormalities being more prominent and involving all chromosomes. The following two 3p abnormalities were detected in one patient: del(3)(p22) and t(3;5)(p25;q31). FISH showed positive results regarding FHIT deletion in 9 (30%) ADC, and 7 (29%) SCLC patients.

Conclusion: Regardless of the rarity of 3p abnormalities in lymphocytes, a high frequency of chromosomal aberrations may indicate genomic instability. Nevertheless, due to being a time-consuming and expertise-requiring technique, conventional cytogenetics is not recommended for lung cancer monitoring. However, the FISH results suggested that using FISH to examine FHIT gene status in lymphocytes could be a promising biomarker for lung cancer.

Keywords: Small cell lung carcinoma, FISH technique, adenocarcinoma, cytogenetics, chromosome 3, FHIT

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APA

Bakhshaliyeva, N., Çırakoğlu, A., Öngen, H.G., Bil Tuncay, E., & Tarkan Argüden, Y. (2024). Cytogenetic and FISH Examination of 3p Abnormalities in Lung Cancer Patients. Experimed, 14(1), 26-39. https://doi.org/10.26650/experimed.1408284


AMA

Bakhshaliyeva N, Çırakoğlu A, Öngen H G, Bil Tuncay E, Tarkan Argüden Y. Cytogenetic and FISH Examination of 3p Abnormalities in Lung Cancer Patients. Experimed. 2024;14(1):26-39. https://doi.org/10.26650/experimed.1408284


ABNT

Bakhshaliyeva, N.; Çırakoğlu, A.; Öngen, H.G.; Bil Tuncay, E.; Tarkan Argüden, Y. Cytogenetic and FISH Examination of 3p Abnormalities in Lung Cancer Patients. Experimed, [Publisher Location], v. 14, n. 1, p. 26-39, 2024.


Chicago: Author-Date Style

Bakhshaliyeva, Narmin, and Ayşe Çırakoğlu and Hürrem Gül Öngen and Esin Bil Tuncay and Yelda Tarkan Argüden. 2024. “Cytogenetic and FISH Examination of 3p Abnormalities in Lung Cancer Patients.” Experimed 14, no. 1: 26-39. https://doi.org/10.26650/experimed.1408284


Chicago: Humanities Style

Bakhshaliyeva, Narmin, and Ayşe Çırakoğlu and Hürrem Gül Öngen and Esin Bil Tuncay and Yelda Tarkan Argüden. “Cytogenetic and FISH Examination of 3p Abnormalities in Lung Cancer Patients.” Experimed 14, no. 1 (May. 2024): 26-39. https://doi.org/10.26650/experimed.1408284


Harvard: Australian Style

Bakhshaliyeva, N & Çırakoğlu, A & Öngen, HG & Bil Tuncay, E & Tarkan Argüden, Y 2024, 'Cytogenetic and FISH Examination of 3p Abnormalities in Lung Cancer Patients', Experimed, vol. 14, no. 1, pp. 26-39, viewed 3 May. 2024, https://doi.org/10.26650/experimed.1408284


Harvard: Author-Date Style

Bakhshaliyeva, N. and Çırakoğlu, A. and Öngen, H.G. and Bil Tuncay, E. and Tarkan Argüden, Y. (2024) ‘Cytogenetic and FISH Examination of 3p Abnormalities in Lung Cancer Patients’, Experimed, 14(1), pp. 26-39. https://doi.org/10.26650/experimed.1408284 (3 May. 2024).


MLA

Bakhshaliyeva, Narmin, and Ayşe Çırakoğlu and Hürrem Gül Öngen and Esin Bil Tuncay and Yelda Tarkan Argüden. “Cytogenetic and FISH Examination of 3p Abnormalities in Lung Cancer Patients.” Experimed, vol. 14, no. 1, 2024, pp. 26-39. [Database Container], https://doi.org/10.26650/experimed.1408284


Vancouver

Bakhshaliyeva N, Çırakoğlu A, Öngen HG, Bil Tuncay E, Tarkan Argüden Y. Cytogenetic and FISH Examination of 3p Abnormalities in Lung Cancer Patients. Experimed [Internet]. 3 May. 2024 [cited 3 May. 2024];14(1):26-39. Available from: https://doi.org/10.26650/experimed.1408284 doi: 10.26650/experimed.1408284


ISNAD

Bakhshaliyeva, Narmin - Çırakoğlu, Ayşe - Öngen, HürremGül - Bil Tuncay, Esin - Tarkan Argüden, Yelda. “Cytogenetic and FISH Examination of 3p Abnormalities in Lung Cancer Patients”. Experimed 14/1 (May. 2024): 26-39. https://doi.org/10.26650/experimed.1408284



TIMELINE


Submitted23.12.2023
Accepted13.02.2024
Published Online25.03.2024

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