Research Article

DOI :10.26650/experimed.1524088 IUP :10.26650/experimed.1524088 Full Text (PDF)

Establishing Age-Gender Specific Reference Values of T and B Lymphocyte Subsets in Healthy Adults Using Flow Cytometry

Fatma Turgut Kaynak, Melek Yanaşık, Suzan Adın Çınar, Sevgi Kalayoğlu Beşışık, Günnur Deniz, Metin Yusuf Gelmez

Objective: Geographical, age and gender differences possibly affect the distribution of lymphocyte subsets. This study aimed to determine the distribution of T and B lymphocytes and their subsets by evaluating a larger healthy adult population in terms of gender and age.

Materials and Methods: Blood samples from 118 healthy adults aged 20-60 years were evaluated using flow cytometry.

Results: B cell subsets were not affected by age and gender differences; however, CD3⁺ and CD4⁺ T cell ratios were higher in women than in men. Gender and age differences did not affect the distribution of CD4⁺ T cell subsets, but increased effector memory CD45RA⁺ CD8⁺ T and decreased naive CD8⁺ T were found among the group aged between 50-60 years. Additionally, regulatory T cells were found at higher levels in individuals aged between 50-60 years. Moreover, high follicular regulatory T cells in men and low follicular helper T cells in women were observed in individuals aged 50-60 years. Advanced age in females may affect the differentiation of CD4⁺ T cells to T helper type 1.

Conclusion: Our results indicate that age and gender differences may affect cell distribution. Our findings may contribute to the clinical evaluation of lymphocyte subset distributions as diagnostic tests performed using flow cytometry for early diagnosis and appropriate treatment of patients.

Keywords: T lymphocytes, B lymphocytes, Flow cytometry, Reference values of lymphocyte subsets

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APA

Turgut Kaynak, F., Yanaşık, M., Adın Çınar, S., Kalayoğlu Beşışık, S., Deniz, G., & Gelmez, M.Y. (2020). Establishing Age-Gender Specific Reference Values of T and B Lymphocyte Subsets in Healthy Adults Using Flow Cytometry. Experimed, 0(0), -. https://doi.org/10.26650/experimed.1524088

AMA

Turgut Kaynak F, Yanaşık M, Adın Çınar S, Kalayoğlu Beşışık S, Deniz G, Gelmez M Y. Establishing Age-Gender Specific Reference Values of T and B Lymphocyte Subsets in Healthy Adults Using Flow Cytometry. Experimed. 2020;0(0):-. https://doi.org/10.26650/experimed.1524088

ABNT

Turgut Kaynak, F.; Yanaşık, M.; Adın Çınar, S.; Kalayoğlu Beşışık, S.; Deniz, G.; Gelmez, M.Y. Establishing Age-Gender Specific Reference Values of T and B Lymphocyte Subsets in Healthy Adults Using Flow Cytometry. Experimed, [Publisher Location], v. 0, n. 0, p. -, 2020.

Chicago: Author-Date Style

Turgut Kaynak, Fatma, and Melek Yanaşık and Suzan Adın Çınar and Sevgi Kalayoğlu Beşışık and Günnur Deniz and Metin Yusuf Gelmez. 2020. “Establishing Age-Gender Specific Reference Values of T and B Lymphocyte Subsets in Healthy Adults Using Flow Cytometry.” Experimed 0, no. 0: -. https://doi.org/10.26650/experimed.1524088

Chicago: Humanities Style

Turgut Kaynak, Fatma, and Melek Yanaşık and Suzan Adın Çınar and Sevgi Kalayoğlu Beşışık and Günnur Deniz and Metin Yusuf Gelmez. “Establishing Age-Gender Specific Reference Values of T and B Lymphocyte Subsets in Healthy Adults Using Flow Cytometry.” Experimed 0, no. 0 (Apr. 2025): -. https://doi.org/10.26650/experimed.1524088

Harvard: Australian Style

Turgut Kaynak, F & Yanaşık, M & Adın Çınar, S & Kalayoğlu Beşışık, S & Deniz, G & Gelmez, MY 2020, 'Establishing Age-Gender Specific Reference Values of T and B Lymphocyte Subsets in Healthy Adults Using Flow Cytometry', Experimed, vol. 0, no. 0, pp. -, viewed 3 Apr. 2025, https://doi.org/10.26650/experimed.1524088

Harvard: Author-Date Style

Turgut Kaynak, F. and Yanaşık, M. and Adın Çınar, S. and Kalayoğlu Beşışık, S. and Deniz, G. and Gelmez, M.Y. (2020) ‘Establishing Age-Gender Specific Reference Values of T and B Lymphocyte Subsets in Healthy Adults Using Flow Cytometry’, Experimed, 0(0), pp. -. https://doi.org/10.26650/experimed.1524088 (3 Apr. 2025).

MLA

Turgut Kaynak, Fatma, and Melek Yanaşık and Suzan Adın Çınar and Sevgi Kalayoğlu Beşışık and Günnur Deniz and Metin Yusuf Gelmez. “Establishing Age-Gender Specific Reference Values of T and B Lymphocyte Subsets in Healthy Adults Using Flow Cytometry.” Experimed, vol. 0, no. 0, 2020, pp. -. [Database Container], https://doi.org/10.26650/experimed.1524088

Vancouver

Turgut Kaynak F, Yanaşık M, Adın Çınar S, Kalayoğlu Beşışık S, Deniz G, Gelmez MY. Establishing Age-Gender Specific Reference Values of T and B Lymphocyte Subsets in Healthy Adults Using Flow Cytometry. Experimed [Internet]. 3 Apr. 2025 [cited 3 Apr. 2025];0(0):-. Available from: https://doi.org/10.26650/experimed.1524088 doi: 10.26650/experimed.1524088

ISNAD

Turgut Kaynak, Fatma - Yanaşık, Melek - Adın Çınar, Suzan - Kalayoğlu Beşışık, Sevgi - Deniz, Günnur - Gelmez, MetinYusuf. “Establishing Age-Gender Specific Reference Values of T and B Lymphocyte Subsets in Healthy Adults Using Flow Cytometry”. Experimed 0/0 (Apr. 2025): -. https://doi.org/10.26650/experimed.1524088

TIMELINE

Submitted	30.07.2024
Accepted	08.10.2024
Published Online	12.03.2025

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