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DOI :10.26650/experimed.1500633   IUP :10.26650/experimed.1500633    Full Text (PDF)

Evaluation of the Molecular Effects of the Anticancer Adjuvant Valproic Acid on HEK293T Cells

Hazal Banu Olgun Çelebioğlu, Fatma Yeliz Ekici, Abdullah Yılmaz, Feyza Nur Tuncer

Objective: Valproic acid (VPA) can induce apoptosis while inhibiting proliferation in various cancer cells. This study adopted a novel strategy for investigating the molecular effects of VPA on non-cancerous cells. Building upon our previous work, which examined the effects of VPA in PANC-1 cells, we now turn our attention to HEK293T cells to determine the effect of VPA in non-cancerous cells.

Materials and Methods: HEK293T cells were treated with 2.5 and 5-mM VPA. Flow cytometry measurements were performed on the 24th, 48th, 72nd, and 96th h. Assays for apoptosis and proliferation were conducted using annexin V/ propidium iodide (PI) staining and carboxyfluorescein-succinimidyl ester (CFSE), respectively.

Results: No statistical significance was detected between the two different doses of VPA-treated cells and the controls at any time point. While early apoptosis values remained stable until 72 h in all groups, an increase was monitored in cells subjected to VPA for 96 h.

Conclusion: Preliminary results indicated no proliferative effect of VPA treatment. However, it may induce apoptosis in long-term incubations. Nevertheless, additional doses of VPA at increased concentrations should be administered to explore cytotoxic levels and their impact on proliferation and apoptosis.

Keywords: HEK293T, VPA, apoptosis, proliferation

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APA

Olgun Çelebioğlu, H.B., Ekici, F., Yılmaz, A., & Tuncer, F.N. (2024). Evaluation of the Molecular Effects of the Anticancer Adjuvant Valproic Acid on HEK293T Cells. Experimed, 14(3), 154-160. https://doi.org/10.26650/experimed.1500633


AMA

Olgun Çelebioğlu H B, Ekici F, Yılmaz A, Tuncer F N. Evaluation of the Molecular Effects of the Anticancer Adjuvant Valproic Acid on HEK293T Cells. Experimed. 2024;14(3):154-160. https://doi.org/10.26650/experimed.1500633


ABNT

Olgun Çelebioğlu, H.B.; Ekici, F.; Yılmaz, A.; Tuncer, F.N. Evaluation of the Molecular Effects of the Anticancer Adjuvant Valproic Acid on HEK293T Cells. Experimed, [Publisher Location], v. 14, n. 3, p. 154-160, 2024.


Chicago: Author-Date Style

Olgun Çelebioğlu, Hazal Banu, and Fatma Yeliz Ekici and Abdullah Yılmaz and Feyza Nur Tuncer. 2024. “Evaluation of the Molecular Effects of the Anticancer Adjuvant Valproic Acid on HEK293T Cells.” Experimed 14, no. 3: 154-160. https://doi.org/10.26650/experimed.1500633


Chicago: Humanities Style

Olgun Çelebioğlu, Hazal Banu, and Fatma Yeliz Ekici and Abdullah Yılmaz and Feyza Nur Tuncer. “Evaluation of the Molecular Effects of the Anticancer Adjuvant Valproic Acid on HEK293T Cells.” Experimed 14, no. 3 (Dec. 2024): 154-160. https://doi.org/10.26650/experimed.1500633


Harvard: Australian Style

Olgun Çelebioğlu, HB & Ekici, F & Yılmaz, A & Tuncer, FN 2024, 'Evaluation of the Molecular Effects of the Anticancer Adjuvant Valproic Acid on HEK293T Cells', Experimed, vol. 14, no. 3, pp. 154-160, viewed 21 Dec. 2024, https://doi.org/10.26650/experimed.1500633


Harvard: Author-Date Style

Olgun Çelebioğlu, H.B. and Ekici, F. and Yılmaz, A. and Tuncer, F.N. (2024) ‘Evaluation of the Molecular Effects of the Anticancer Adjuvant Valproic Acid on HEK293T Cells’, Experimed, 14(3), pp. 154-160. https://doi.org/10.26650/experimed.1500633 (21 Dec. 2024).


MLA

Olgun Çelebioğlu, Hazal Banu, and Fatma Yeliz Ekici and Abdullah Yılmaz and Feyza Nur Tuncer. “Evaluation of the Molecular Effects of the Anticancer Adjuvant Valproic Acid on HEK293T Cells.” Experimed, vol. 14, no. 3, 2024, pp. 154-160. [Database Container], https://doi.org/10.26650/experimed.1500633


Vancouver

Olgun Çelebioğlu HB, Ekici F, Yılmaz A, Tuncer FN. Evaluation of the Molecular Effects of the Anticancer Adjuvant Valproic Acid on HEK293T Cells. Experimed [Internet]. 21 Dec. 2024 [cited 21 Dec. 2024];14(3):154-160. Available from: https://doi.org/10.26650/experimed.1500633 doi: 10.26650/experimed.1500633


ISNAD

Olgun Çelebioğlu, HazalBanu - Ekici, Fatma Yeliz - Yılmaz, Abdullah - Tuncer, FeyzaNur. “Evaluation of the Molecular Effects of the Anticancer Adjuvant Valproic Acid on HEK293T Cells”. Experimed 14/3 (Dec. 2024): 154-160. https://doi.org/10.26650/experimed.1500633



TIMELINE


Submitted13.07.2024
Accepted25.11.2024
Published Online29.11.2024

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