Identification of Peptides Binding Specifically to  Colon Cancer Cells through Phage Display Peptide  Library Screening

Şahin Deniz; Kanlıdere Zeynep; Ekal Barışcan; Abaylı Berkehür; Hayat Yordan; Selçuk Ece

doi:https://dx.doi.org/10.26650/experimed.1500881

Research Article

DOI :10.26650/experimed.1500881 IUP :10.26650/experimed.1500881 Full Text (PDF)

Identification of Peptides Binding Specifically to Colon Cancer Cells through Phage Display Peptide Library Screening

Deniz Şahin, Zeynep Kanlıdere, Barışcan Ekal, Berkehür Abaylı, Yordan Hayat, Ece Selçuk

Objective: Colorectal cancer ranks among the most frequently occurring cancers. Addressing the diagnosis and treatment challenges requires the advancement of alternative approaches that can more precisely differentiate between cancerous and healthy cells.

Materials and Methods: We identified colon cancer cell surface specific 12-mer peptides using subtractive phage display technique. Four rounds of biopanning were conducted using the M13 bacteriophage PhD-12 peptide library with the human colon cancer cell line HCT-116. Subtractive selection involved the use of the human rectal cancer cell line SW837 and the healthy CCD112-CoN cell line. Binding levels of selected phage clones were tested by ELISA-HRP binding assays. Experimentally identified peptide sequences were synthesized by solid-phase chemistry. Peptides were labelled at the N-terminus with a fluorescent dye for visualization of cells by confocal microscopy.

Results: Peptide COL419 (Serine-Valine-Alanine-Histidine-Leucine-Serine-Proline-Histidine-Serine-Threonine-Threonine-Alanine; SVAHLSPHSTTA) can differentiate colon and rectal cancers from healthy cells. In addition, the COL-AA peptide (Histidine-Tyrosine-Proline Threonine-Asparagine-Leucine-Histidine-Tyrosine-Proline-Threonine-Asparagine-Leucine; HYPTNLHYPTNL), which was designed from the amino acid composition analysis of the experimentally selected peptide sequences bound to colon cancer cells and neither rectal cancer cells nor healthy colorectal cells.

Conclusion: The peptides determined specifically target colon cancer cells. These findings enhance our understanding of the differences between these cancer types. The selected peptides have the potential for clinical application in targeted diagnosis and treatment of colon cancer.

Keywords: Colon cancer, peptides, phage display, rectal cancer, targeted approaches

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APA

Şahin, D., Kanlıdere, Z., Ekal, B., Abaylı, B., Hayat, Y., & Selçuk, E. (2024). Identification of Peptides Binding Specifically to Colon Cancer Cells through Phage Display Peptide Library Screening. Experimed, 14(3), 174-182. https://doi.org/10.26650/experimed.1500881

AMA

Şahin D, Kanlıdere Z, Ekal B, Abaylı B, Hayat Y, Selçuk E. Identification of Peptides Binding Specifically to Colon Cancer Cells through Phage Display Peptide Library Screening. Experimed. 2024;14(3):174-182. https://doi.org/10.26650/experimed.1500881

ABNT

Şahin, D.; Kanlıdere, Z.; Ekal, B.; Abaylı, B.; Hayat, Y.; Selçuk, E. Identification of Peptides Binding Specifically to Colon Cancer Cells through Phage Display Peptide Library Screening. Experimed, [Publisher Location], v. 14, n. 3, p. 174-182, 2024.

Chicago: Author-Date Style

Şahin, Deniz, and Zeynep Kanlıdere and Barışcan Ekal and Berkehür Abaylı and Yordan Hayat and Ece Selçuk. 2024. “Identification of Peptides Binding Specifically to Colon Cancer Cells through Phage Display Peptide Library Screening.” Experimed 14, no. 3: 174-182. https://doi.org/10.26650/experimed.1500881

Chicago: Humanities Style

Şahin, Deniz, and Zeynep Kanlıdere and Barışcan Ekal and Berkehür Abaylı and Yordan Hayat and Ece Selçuk. “Identification of Peptides Binding Specifically to Colon Cancer Cells through Phage Display Peptide Library Screening.” Experimed 14, no. 3 (Dec. 2024): 174-182. https://doi.org/10.26650/experimed.1500881

Harvard: Australian Style

Şahin, D & Kanlıdere, Z & Ekal, B & Abaylı, B & Hayat, Y & Selçuk, E 2024, 'Identification of Peptides Binding Specifically to Colon Cancer Cells through Phage Display Peptide Library Screening', Experimed, vol. 14, no. 3, pp. 174-182, viewed 21 Dec. 2024, https://doi.org/10.26650/experimed.1500881

Harvard: Author-Date Style

Şahin, D. and Kanlıdere, Z. and Ekal, B. and Abaylı, B. and Hayat, Y. and Selçuk, E. (2024) ‘Identification of Peptides Binding Specifically to Colon Cancer Cells through Phage Display Peptide Library Screening’, Experimed, 14(3), pp. 174-182. https://doi.org/10.26650/experimed.1500881 (21 Dec. 2024).

MLA

Şahin, Deniz, and Zeynep Kanlıdere and Barışcan Ekal and Berkehür Abaylı and Yordan Hayat and Ece Selçuk. “Identification of Peptides Binding Specifically to Colon Cancer Cells through Phage Display Peptide Library Screening.” Experimed, vol. 14, no. 3, 2024, pp. 174-182. [Database Container], https://doi.org/10.26650/experimed.1500881

Vancouver

Şahin D, Kanlıdere Z, Ekal B, Abaylı B, Hayat Y, Selçuk E. Identification of Peptides Binding Specifically to Colon Cancer Cells through Phage Display Peptide Library Screening. Experimed [Internet]. 21 Dec. 2024 [cited 21 Dec. 2024];14(3):174-182. Available from: https://doi.org/10.26650/experimed.1500881 doi: 10.26650/experimed.1500881

ISNAD

Şahin, Deniz - Kanlıdere, Zeynep - Ekal, Barışcan - Abaylı, Berkehür - Hayat, Yordan - Selçuk, Ece. “Identification of Peptides Binding Specifically to Colon Cancer Cells through Phage Display Peptide Library Screening”. Experimed 14/3 (Dec. 2024): 174-182. https://doi.org/10.26650/experimed.1500881

Volume 14, Issue 32024, P. 174-182

TIMELINE

Submitted	27.06.2024
Accepted	04.10.2024
Published Online	02.12.2024

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