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DOI :10.26650/experimed.1456373   IUP :10.26650/experimed.1456373    Full Text (PDF)

Virulence Factor of Porphyromonas gingivalis Disrupts Intestinal Oxidative Status and Acetylcholinesterase Activity in Rotenone-Exposed Zebrafish

İpek İşlek, Merih Beler, İsmail Ünal, Derya Cansız, Ebru Emekli Alturfan, Kemal Naci Köse

Objective: Porphyromonas gingivalis (P. gingivalis) is a major pathogenic bacterium in periodontal disease and is associated with neurodegenerative diseases. One of the most destructive endotoxins of P. gingivalis is gingipain. Our objective was to show the impact of gingipain on acetylcholinesterase (AChE) activity and oxidative status in the intestinal tissues of zebrafish exposed to rotenone.

Materials and Methods: Zebrafish were grouped as; control group (C), gingipain-injected group (G), rotenone-exposed group (R), gingipain-injected and rotenone-exposed group (G+R) (n=15). At the end of 4 weeks, spectrophotometric analyses were performed to evaluate the oxidative status and AChE activity in the intestinal tissues.

Results: Intestinal lipid peroxidation (LPO) levels were higher in the G group than in the C group. Gingipain injection significantly reduced the activities of AChE and superoxide dismutase (SOD). In the R group, there were significant elevations in SOD, nitric oxide (NO), glutathione-S-transferase (GST), and AChE activities compared with those in the C group. In the G+R group, LPO, NO, SOD, and GST activities were reduced compared with the R group.

Conclusion: Our results show that gingipain dysregulated AChE activity and the oxidant-antioxidant balance in rotenone-exposed zebrafish, demonstrating its possible role in gut dysbiosis, neuroinflammation, and Parkinson’s disease.

Keywords: Porphyromonas gingivalis, gingipain, zebrafish, rotenone, oxidative stress, acetylcholinesterase

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APA

İşlek, İ., Beler, M., Ünal, İ., Cansız, D., Emekli Alturfan, E., & Köse, K.N. (2024). Virulence Factor of Porphyromonas gingivalis Disrupts Intestinal Oxidative Status and Acetylcholinesterase Activity in Rotenone-Exposed Zebrafish. Experimed, 14(3), 139-145. https://doi.org/10.26650/experimed.1456373


AMA

İşlek İ, Beler M, Ünal İ, Cansız D, Emekli Alturfan E, Köse K N. Virulence Factor of Porphyromonas gingivalis Disrupts Intestinal Oxidative Status and Acetylcholinesterase Activity in Rotenone-Exposed Zebrafish. Experimed. 2024;14(3):139-145. https://doi.org/10.26650/experimed.1456373


ABNT

İşlek, İ.; Beler, M.; Ünal, İ.; Cansız, D.; Emekli Alturfan, E.; Köse, K.N. Virulence Factor of Porphyromonas gingivalis Disrupts Intestinal Oxidative Status and Acetylcholinesterase Activity in Rotenone-Exposed Zebrafish. Experimed, [Publisher Location], v. 14, n. 3, p. 139-145, 2024.


Chicago: Author-Date Style

İşlek, İpek, and Merih Beler and İsmail Ünal and Derya Cansız and Ebru Emekli Alturfan and Kemal Naci Köse. 2024. “Virulence Factor of Porphyromonas gingivalis Disrupts Intestinal Oxidative Status and Acetylcholinesterase Activity in Rotenone-Exposed Zebrafish.” Experimed 14, no. 3: 139-145. https://doi.org/10.26650/experimed.1456373


Chicago: Humanities Style

İşlek, İpek, and Merih Beler and İsmail Ünal and Derya Cansız and Ebru Emekli Alturfan and Kemal Naci Köse. “Virulence Factor of Porphyromonas gingivalis Disrupts Intestinal Oxidative Status and Acetylcholinesterase Activity in Rotenone-Exposed Zebrafish.” Experimed 14, no. 3 (Dec. 2024): 139-145. https://doi.org/10.26650/experimed.1456373


Harvard: Australian Style

İşlek, İ & Beler, M & Ünal, İ & Cansız, D & Emekli Alturfan, E & Köse, KN 2024, 'Virulence Factor of Porphyromonas gingivalis Disrupts Intestinal Oxidative Status and Acetylcholinesterase Activity in Rotenone-Exposed Zebrafish', Experimed, vol. 14, no. 3, pp. 139-145, viewed 21 Dec. 2024, https://doi.org/10.26650/experimed.1456373


Harvard: Author-Date Style

İşlek, İ. and Beler, M. and Ünal, İ. and Cansız, D. and Emekli Alturfan, E. and Köse, K.N. (2024) ‘Virulence Factor of Porphyromonas gingivalis Disrupts Intestinal Oxidative Status and Acetylcholinesterase Activity in Rotenone-Exposed Zebrafish’, Experimed, 14(3), pp. 139-145. https://doi.org/10.26650/experimed.1456373 (21 Dec. 2024).


MLA

İşlek, İpek, and Merih Beler and İsmail Ünal and Derya Cansız and Ebru Emekli Alturfan and Kemal Naci Köse. “Virulence Factor of Porphyromonas gingivalis Disrupts Intestinal Oxidative Status and Acetylcholinesterase Activity in Rotenone-Exposed Zebrafish.” Experimed, vol. 14, no. 3, 2024, pp. 139-145. [Database Container], https://doi.org/10.26650/experimed.1456373


Vancouver

İşlek İ, Beler M, Ünal İ, Cansız D, Emekli Alturfan E, Köse KN. Virulence Factor of Porphyromonas gingivalis Disrupts Intestinal Oxidative Status and Acetylcholinesterase Activity in Rotenone-Exposed Zebrafish. Experimed [Internet]. 21 Dec. 2024 [cited 21 Dec. 2024];14(3):139-145. Available from: https://doi.org/10.26650/experimed.1456373 doi: 10.26650/experimed.1456373


ISNAD

İşlek, İpek - Beler, Merih - Ünal, İsmail - Cansız, Derya - Emekli Alturfan, Ebru - Köse, KemalNaci. “Virulence Factor of Porphyromonas gingivalis Disrupts Intestinal Oxidative Status and Acetylcholinesterase Activity in Rotenone-Exposed Zebrafish”. Experimed 14/3 (Dec. 2024): 139-145. https://doi.org/10.26650/experimed.1456373



TIMELINE


Submitted21.03.2024
Accepted13.05.2024
Published Online19.12.2024

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